Abstract:
:Optical imaging has become a powerful tool for studying brains in vivo. The opacity of adult brains makes microendoscopy, with an optical probe such as a gradient index (GRIN) lens embedded into brain tissue to provide optical relay, the method of choice for imaging neurons and neural activity in deeply buried brain structures. Incorporating a Bessel focus scanning module into two-photon fluorescence microendoscopy, we extended the excitation focus axially and improved its lateral resolution. Scanning the Bessel focus in 2D, we imaged volumes of neurons at high-throughput while resolving fine structures such as synaptic terminals. We applied this approach to the volumetric anatomical imaging of dendritic spines and axonal boutons in the mouse hippocampus, and functional imaging of GABAergic neurons in the mouse lateral hypothalamus in vivo.
journal_name
Elifejournal_title
eLifeauthors
Meng G,Liang Y,Sarsfield S,Jiang WC,Lu R,Dudman JT,Aponte Y,Ji Ndoi
10.7554/eLife.40805subject
Has Abstractpub_date
2019-01-04 00:00:00issn
2050-084Xpii
40805journal_volume
8pub_type
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