Casein kinase 1 family proteins promote Slimb-dependent Expanded degradation.

abstract：:The growth of preprints in the life sciences has been reported widely and is driving policy changes for journals and funders, but little quantitative information has been published about preprint usage. Here, we report how we collected and analyzed data on all 37,648 preprints uploaded to bioRxiv.org, the largest biol...

journal_title：eLife

authors： Abdill RJ,Blekhman R

更新日期：2019-04-24 00:00:00

abstract：:Anxiety results in sub-optimal motor learning, but the precise mechanisms through which this effect occurs remain unknown. Using a motor sequence learning paradigm with separate phases for initial exploration and reward-based learning, we show that anxiety states in humans impair learning by attenuating the update of ...

journal_title：eLife

authors： Sporn S,Hein T,Herrojo Ruiz M

更新日期：2020-05-19 00:00:00

abstract：:Ten countries have reported pfhrp2/pfhrp3 gene deletions since the first observation of pfhrp2-deleted parasites in 2012. In a previous study (Watson et al., 2017), we characterised the drivers selecting for pfhrp2/3 deletions and mapped the regions in Africa with the greatest selection pressure. In February 2018, the...

journal_title：eLife

authors： Watson OJ,Verity R,Ghani AC,Garske T,Cunningham J,Tshefu A,Mwandagalirwa MK,Meshnick SR,Parr JB,Slater HC

更新日期：2019-05-02 00:00:00

abstract：:A polymorphism in the autophagy gene Atg16l1 is associated with susceptibility to inflammatory bowel disease (IBD); however, it remains unclear how autophagy contributes to intestinal immune homeostasis. Here, we demonstrate that autophagy is essential for maintenance of balanced CD4(+) T cell responses in the intesti...

journal_title：eLife

authors： Kabat AM,Harrison OJ,Riffelmacher T,Moghaddam AE,Pearson CF,Laing A,Abeler-Dörner L,Forman SP,Grencis RK,Sattentau Q,Simon AK,Pott J,Maloy KJ

更新日期：2016-02-24 00:00:00

abstract：:Macrophage activation/polarization to distinct functional states is critically supported by metabolic shifts. How polarizing signals coordinate metabolic and functional reprogramming, and the potential implications for control of macrophage activation, remains poorly understood. Here we show that IL-4 signaling co-opt...

journal_title：eLife

authors： Covarrubias AJ,Aksoylar HI,Yu J,Snyder NW,Worth AJ,Iyer SS,Wang J,Ben-Sahra I,Byles V,Polynne-Stapornkul T,Espinosa EC,Lamming D,Manning BD,Zhang Y,Blair IA,Horng T

更新日期：2016-02-19 00:00:00

abstract：:Mitochondria are dynamic organelles that must precisely control their protein composition according to cellular energy demand. Although nuclear-encoded mRNAs can be localized to the mitochondrial surface, the importance of this localization is unclear. As yeast switch to respiratory metabolism, there is an increase in...

journal_title：eLife

authors： Tsuboi T,Viana MP,Xu F,Yu J,Chanchani R,Arceo XG,Tutucci E,Choi J,Chen YS,Singer RH,Rafelski SM,Zid BM

更新日期：2020-08-07 00:00:00

abstract：:TCF/LEF factors are ancient context-dependent enhancer-binding proteins that are activated by β-catenin following Wnt signaling. They control embryonic development and adult stem cell compartments, and their dysregulation often causes cancer. β-catenin-dependent transcription relies on the NPF motif of Pygo proteins. ...

journal_title：eLife

authors： Fiedler M,Graeb M,Mieszczanek J,Rutherford TJ,Johnson CM,Bienz M

更新日期：2015-08-27 00:00:00

abstract：:Numerous brain regions have been shown to have neural correlates of gradually accumulating evidence for decision-making, but the causal roles of these regions in decisions driven by accumulation of evidence have yet to be determined. Here, in rats performing an auditory evidence accumulation task, we inactivated the f...

journal_title：eLife

authors： Erlich JC,Brunton BW,Duan CA,Hanks TD,Brody CD

更新日期：2015-04-14 00:00:00

abstract：:The investigation of prosocial behavior is of particular interest from an evolutionary perspective. Comparisons of prosociality across non-human animal species have, however, so far largely focused on primates, and their interpretation is hampered by the diversity of paradigms and procedures used. Here, we present the...

journal_title：eLife

authors： Horn L,Bugnyar T,Griesser M,Hengl M,Izawa EI,Oortwijn T,Rössler C,Scheer C,Schiestl M,Suyama M,Taylor AH,Vanhooland LC,von Bayern AM,Zürcher Y,Massen JJ

更新日期：2020-10-20 00:00:00

abstract：:Homologous recombination is widespread and catalyzes evolution. Nonetheless, its existence in animal mitochondrial DNA is questioned. We designed selections for recombination between co-resident mitochondrial genomes in various heteroplasmic Drosophila lines. In four experimental settings, recombinant genomes became t...

journal_title：eLife

authors： Ma H,O'Farrell PH

更新日期：2015-08-03 00:00:00

abstract：:Variation in the presentation of hereditary immunodeficiencies may be explained by genetic or environmental factors. Patients with mutations in HOIL1 (RBCK1) present with amylopectinosis-associated myopathy with or without hyper-inflammation and immunodeficiency. We report that barrier-raised HOIL-1-deficient mice exh...

journal_title：eLife

authors： MacDuff DA,Reese TA,Kimmey JM,Weiss LA,Song C,Zhang X,Kambal A,Duan E,Carrero JA,Boisson B,Laplantine E,Israel A,Picard C,Colonna M,Edelson BT,Sibley LD,Stallings CL,Casanova JL,Iwai K,Virgin HW

更新日期：2015-01-20 00:00:00

abstract：:We determined differential gene expression in response to high glucose in lymphoblastoid cell lines derived from matched individuals with type 1 diabetes with and without retinopathy. Those genes exhibiting the largest difference in glucose response were assessed for association with diabetic retinopathy in a genome-w...

journal_title：eLife

authors： Skol AD,Jung SC,Sokovic AM,Chen S,Fazal S,Sosina O,Borkar PP,Lin A,Sverdlov M,Cao D,Swaroop A,Bebu I,DCCT\/EDIC Study group.,Stranger BE,Grassi MA

更新日期：2020-11-09 00:00:00

abstract：:Lamina-associated polypeptide 1 (LAP1) resides at the nuclear envelope and interacts with Torsins, poorly understood endoplasmic reticulum (ER)-localized AAA+ ATPases, through a conserved, perinuclear domain. We determined the crystal structure of the perinuclear domain of human LAP1. LAP1 possesses an atypical AAA+ f...

journal_title：eLife

authors： Sosa BA,Demircioglu FE,Chen JZ,Ingram J,Ploegh HL,Schwartz TU

更新日期：2014-08-22 00:00:00

abstract：:The production of action sequences is a fundamental aspect of motor skills. To examine whether primary motor cortex (M1) is involved in maintenance of sequential movements, we trained two monkeys (Cebus apella) to perform two sequential reaching tasks. In one task, sequential movements were instructed by visual cues, ...

journal_title：eLife

authors： Ohbayashi M

更新日期：2020-02-10 00:00:00

abstract：:The training of PhD students and early-career scientists is largely an apprenticeship in which the trainee associates with an expert to become an independent scientist. But when is a PhD student ready to graduate, a postdoctoral scholar ready for an independent position, or an early-career scientist ready for advanced...

journal_title：eLife

authors： Verderame MF,Freedman VH,Kozlowski LM,McCormack WT

更新日期：2018-05-31 00:00:00

abstract：:Viral replicase recruitment and long-range RNA interactions are essential for RNA virus replication, yet the mechanism of their interplay remains elusive. Flaviviruses include numerous important human pathogens, e.g., dengue virus (DENV) and Zika virus (ZIKV). Here, we revealed a highly conserved, conformation-tunable...

journal_title：eLife

authors： Liu ZY,Li XF,Jiang T,Deng YQ,Ye Q,Zhao H,Yu JY,Qin CF

更新日期：2016-10-01 00:00:00

abstract：:During early embryogenesis, cells must exit pluripotency and commit to multiple lineages in all germ-layers. How this transition is operated in vivo is poorly understood. Here, we report that MEK1 and the Nanog-related transcription factor Ventx2 coordinate this transition. MEK1 was required to make Xenopus pluripoten...

journal_title：eLife

authors： Scerbo P,Marchal L,Kodjabachian L

更新日期：2017-06-27 00:00:00

abstract：:Key to CRISPR-Cas adaptive immunity is maintaining an ongoing record of invading nucleic acids, a process carried out by the Cas1-Cas2 complex that integrates short segments of foreign genetic material (spacers) into the CRISPR locus. It is hypothesized that Cas1 evolved from casposases, a novel class of transposases....

journal_title：eLife

authors： Hickman AB,Kailasan S,Genzor P,Haase AD,Dyda F

更新日期：2020-01-08 00:00:00

abstract：:Tumors are composed of many different cell types including cancer cells, fibroblasts, and immune cells. Dissecting functional metabolic differences between cell types within a mixed population can be challenging due to the rapid turnover of metabolites relative to the time needed to isolate cells. To overcome this cha...

journal_title：eLife

authors： Lau AN,Li Z,Danai LV,Westermark AM,Darnell AM,Ferreira R,Gocheva V,Sivanand S,Lien EC,Sapp KM,Mayers JR,Biffi G,Chin CR,Davidson SM,Tuveson DA,Jacks T,Matheson NJ,Yilmaz O,Vander Heiden MG

更新日期：2020-07-10 00:00:00

abstract：:Deinococcus radiodurans (DR) survives in the presence of hundreds of double-stranded DNA (dsDNA) breaks by efficiently repairing such breaks. RecO, a protein that is essential for the extreme radioresistance of DR, is one of the major recombination mediator proteins in the RecA-loading process in the RecFOR pathway. H...

journal_title：eLife

authors： Hwang J,Kim JY,Kim C,Park S,Joo S,Kim SK,Lee NK

更新日期：2020-04-16 00:00:00

abstract：:A key challenge in antibiotic stewardship is figuring out how to use antibiotics therapeutically without promoting the evolution of antibiotic resistance. Here, we demonstrate proof of concept for an adjunctive therapy that allows intravenous antibiotic treatment without driving the evolution and onward transmission o...

journal_title：eLife

authors： Morley VJ,Kinnear CL,Sim DG,Olson SN,Jackson LM,Hansen E,Usher GA,Showalter SA,Pai MP,Woods RJ,Read AF

更新日期：2020-12-01 00:00:00

abstract：:Previously, we identified ISRIB as a potent inhibitor of the integrated stress response (ISR) and showed that ISRIB makes cells resistant to the effects of eIF2α phosphorylation and enhances long-term memory in rodents (Sidrauski et al., 2013). Here, we show by genome-wide in vivo ribosome profiling that translation o...

journal_title：eLife

authors： Sidrauski C,McGeachy AM,Ingolia NT,Walter P

更新日期：2015-02-26 00:00:00

abstract：:Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and rea...

journal_title：eLife

authors： Rodrigo Albors A,Tazaki A,Rost F,Nowoshilow S,Chara O,Tanaka EM

更新日期：2015-11-14 00:00:00

abstract：:Loss of polarity correlates with progression of epithelial cancers, but how plasma membrane misorganization drives oncogenic transcriptional events remains unclear. The polarity regulators of the Drosophila Scribble (Scrib) module are potent tumor suppressors and provide a model for mechanistic investigation. RNA prof...

journal_title：eLife

authors： Bunker BD,Nellimoottil TT,Boileau RM,Classen AK,Bilder D

更新日期：2015-02-26 00:00:00

abstract：:Aging is characterized by extensive metabolic reprogramming. To identify metabolic pathways associated with aging, we analyzed age-dependent changes in the metabolomes of long-lived Drosophila melanogaster. Among the metabolites that changed, levels of tyrosine were increased with age in long-lived flies. We demonstra...

journal_title：eLife

authors： Parkhitko AA,Ramesh D,Wang L,Leshchiner D,Filine E,Binari R,Olsen AL,Asara JM,Cracan V,Rabinowitz JD,Brockmann A,Perrimon N

更新日期：2020-12-15 00:00:00

abstract：:Neuroligins (NLGNs) are postsynaptic cell adhesion molecules that interact trans-synaptically with neurexins to mediate synapse development and function. NLGN2 is only at inhibitory synapses while NLGN3 is at both excitatory and inhibitory synapses. We found that NLGN3 function at inhibitory synapses in rat CA1 depend...

journal_title：eLife

authors： Nguyen QA,Horn ME,Nicoll RA

更新日期：2016-11-02 00:00:00

abstract：:Previous studies had shown that the integration of genome wide expression profiles, in metabolic tissues, with genetic and phenotypic variance, provided valuable insight into the underlying molecular mechanisms. We used RNA-Seq to characterize hypothalamic transcriptome in 99 inbred strains of mice from the Hybrid Mou...

journal_title：eLife

authors： Hasin-Brumshtein Y,Khan AH,Hormozdiari F,Pan C,Parks BW,Petyuk VA,Piehowski PD,Brümmer A,Pellegrini M,Xiao X,Eskin E,Smith RD,Lusis AJ,Smith DJ

更新日期：2016-09-13 00:00:00

abstract：:Cancer prevention is predicted to result in more positive therapeutic outcomes than post-diagnostic interventions, and so may be a viable option for future personalized medicine. ...

journal_title：eLife

authors： Komarova NL

更新日期：2015-08-05 00:00:00

abstract：:Voltage-gated sodium channels are targets for a range of pharmaceutical drugs developed for the treatment of neurological diseases. Cannabidiol (CBD), the non-psychoactive compound isolated from cannabis plants, was recently approved for treatment of two types of epilepsy associated with sodium channel mutations. This...

journal_title：eLife

authors： Sait LG,Sula A,Ghovanloo MR,Hollingworth D,Ruben PC,Wallace BA

更新日期：2020-10-22 00:00:00

abstract：:Ligand binding stabilizes different G protein-coupled receptor states via a complex allosteric process that is not completely understood. Here, we have derived free energy landscapes describing activation of the β2 adrenergic receptor bound to ligands with different efficacy profiles using enhanced sampling molecular ...

journal_title：eLife

authors： Fleetwood O,Carlsson J,Delemotte L

更新日期：2021-01-28 00:00:00