A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes.

abstract：:T follicular helper cells (Tfh) are critical for the longevity and quality of antibody-mediated protection against infection. Yet few signaling pathways have been identified to be unique solely to Tfh development. ROQUIN is a post-transcriptional repressor of T cells, acting through its ROQ domain to destabilize mRNA ...

journal_title：eLife

authors： Ramiscal RR,Parish IA,Lee-Young RS,Babon JJ,Blagih J,Pratama A,Martin J,Hawley N,Cappello JY,Nieto PF,Ellyard JI,Kershaw NJ,Sweet RA,Goodnow CC,Jones RG,Febbraio MA,Vinuesa CG,Athanasopoulos V

更新日期：2015-10-23 00:00:00

abstract：:Cytoplasmic dyneins are motor proteins in the AAA+ superfamily that transport cellular cargos toward microtubule minus-ends. Recently, ciliobrevins were reported as selective cell-permeable inhibitors of cytoplasmic dyneins. As is often true for first-in-class inhibitors, the use of ciliobrevins has in part been limit...

journal_title：eLife

authors： Steinman JB,Santarossa CC,Miller RM,Yu LS,Serpinskaya AS,Furukawa H,Morimoto S,Tanaka Y,Nishitani M,Asano M,Zalyte R,Ondrus AE,Johnson AG,Ye F,Nachury MV,Fukase Y,Aso K,Foley MA,Gelfand VI,Chen JK,Carter AP,Kapo

更新日期：2017-05-19 00:00:00

abstract：:Uromodulin is the most abundant protein in the urine. It is exclusively produced by renal epithelial cells and it plays key roles in kidney function and disease. Uromodulin mainly exerts its function as an extracellular matrix whose assembly depends on a conserved, specific proteolytic cleavage leading to conformation...

journal_title：eLife

authors： Brunati M,Perucca S,Han L,Cattaneo A,Consolato F,Andolfo A,Schaeffer C,Olinger E,Peng J,Santambrogio S,Perrier R,Li S,Bokhove M,Bachi A,Hummler E,Devuyst O,Wu Q,Jovine L,Rampoldi L

更新日期：2015-12-17 00:00:00

abstract：:Habit formation is a behavioral adaptation that automates routine actions. Habitual behavior correlates with broad reconfigurations of dorsolateral striatal (DLS) circuit properties that increase gain and shift pathway timing. The mechanism(s) for these circuit adaptations are unknown and could be responsible for habi...

journal_title：eLife

authors： O'Hare JK,Li H,Kim N,Gaidis E,Ade K,Beck J,Yin H,Calakos N

更新日期：2017-09-05 00:00:00

abstract：:Using computer simulations, we generate cell-specific 3D chromosomal structures and compare them to recently published chromatin structures obtained through microscopy. We demonstrate using machine learning and polymer physics simulations that epigenetic information can be used to predict the structural ensembles of m...

journal_title：eLife

authors： Cheng RR,Contessoto VG,Lieberman Aiden E,Wolynes PG,Di Pierro M,Onuchic JN

更新日期：2020-10-13 00:00:00

abstract：:Animals can effortlessly adapt their behavior by generalizing from past aversive experiences, allowing to avoid harm in novel situations. We studied how visual information was sampled by eye-movements during this process called fear generalization, using faces organized along a circular two-dimensional perceptual cont...

journal_title：eLife

authors： Kampermann L,Wilming N,Alink A,Büchel C,Onat S

更新日期：2019-10-22 00:00:00

abstract：:Membrane transporters of the RND superfamily confer multidrug resistance to pathogenic bacteria, and are essential for cholesterol metabolism and embryonic development in humans. We use high-resolution X-ray crystallography and computational methods to delineate the mechanism of the homotrimeric RND-type proton/drug a...

journal_title：eLife

authors： Eicher T,Seeger MA,Anselmi C,Zhou W,Brandstätter L,Verrey F,Diederichs K,Faraldo-Gómez JD,Pos KM

更新日期：2014-09-19 00:00:00

abstract：:Experience-dependent modifications of synaptic connections are thought to change patterns of network activities and stimulus tuning with learning. However, only a few studies explored how synaptic plasticity shapes the response dynamics of cortical circuits. Here, we investigated the mechanism underlying sharpening of...

journal_title：eLife

authors： Lim S

更新日期：2019-08-08 00:00:00

abstract：:Context-dependent memories may guide adaptive behavior relaying in previous experience while updating stored information through reconsolidation. Retrieval can be triggered by partial and shared cues. When the cue is presented, the most relevant memory should be updated. In a contextual version of the object recogniti...

journal_title：eLife

authors： Morici JF,Miranda M,Gallo FT,Zanoni B,Bekinschtein P,Weisstaub NV

更新日期：2018-05-02 00:00:00

abstract：:Slo2 potassium channels play important roles in neuronal function, and their mutations in humans may cause epilepsies and cognitive defects. However, it is largely unknown how Slo2 is regulated by other proteins. Here we show that the function of C. elegans Slo2 (SLO-2) depends on adr-1, a gene important to RNA editin...

journal_title：eLife

authors： Niu LG,Liu P,Wang ZW,Chen B

更新日期：2020-04-21 00:00:00

abstract：:During sleep, the brain experiences large fluctuations in blood volume and altered coupling between neural and vascular signals. ...

journal_title：eLife

authors： Williams SD,Lewis LD

更新日期：2020-12-23 00:00:00

abstract：:To move the body, the brain must precisely coordinate patterns of activity among diverse populations of motor neurons. Here, we use in vivo calcium imaging, electrophysiology, and behavior to understand how genetically-identified motor neurons control flexion of the fruit fly tibia. We find that leg motor neurons exhi...

journal_title：eLife

authors： Azevedo AW,Dickinson ES,Gurung P,Venkatasubramanian L,Mann RS,Tuthill JC

更新日期：2020-06-03 00:00:00

abstract：:RNA-protein (RNP) granules have been proposed to assemble by forming solid RNA/protein aggregates or through phase separation into a liquid RNA/protein phase. Which model describes RNP granules in living cells is still unclear. In this study, we analyze P bodies in budding yeast and find that they have liquid-like pro...

journal_title：eLife

authors： Kroschwald S,Maharana S,Mateju D,Malinovska L,Nüske E,Poser I,Richter D,Alberti S

更新日期：2015-08-04 00:00:00

abstract：:A major challenge in neuroscience is to longitudinally monitor whole brain activity across multiple spatial scales in the same animal. Functional UltraSound (fUS) is an emerging technology that offers images of cerebral blood volume over large brain portions. Here we show for the first time its capability to resolve t...

journal_title：eLife

authors： Bimbard C,Demene C,Girard C,Radtke-Schuller S,Shamma S,Tanter M,Boubenec Y

更新日期：2018-06-28 00:00:00

abstract：:Hepatitis B virus (HBV) is an important but difficult to study human pathogen. Most basics of the hepadnaviral life-cycle were unraveled using duck HBV (DHBV) as a model although DHBV has a capsid protein (CP) comprising ~260 rather than ~180 amino acids. Here we present high-resolution structures of several DHBV caps...

journal_title：eLife

authors： Makbul C,Nassal M,Böttcher B

更新日期：2020-08-14 00:00:00

abstract：:A small molecule called Sm4 can disrupt interactions involving a transcription factor called Sox18, while having little impact on other members of the SoxF family. ...

journal_title：eLife

authors： Kim I,Koh GY

更新日期：2017-01-31 00:00:00

abstract：:Gene transcription can be activated by decreasing the duration of RNA polymerase II pausing in the promoter-proximal region, but how this is achieved remains unclear. Here we use a 'multi-omics' approach to demonstrate that the duration of polymerase pausing generally limits the productive frequency of transcription i...

journal_title：eLife

authors： Gressel S,Schwalb B,Decker TM,Qin W,Leonhardt H,Eick D,Cramer P

更新日期：2017-10-10 00:00:00

abstract：:Bacteria of the genus Shigella cause shigellosis, a severe gastrointestinal disease that is a major cause of diarrhea-associated mortality in humans. Mice are highly resistant to Shigella and the lack of a tractable physiological model of shigellosis has impeded our understanding of this important human disease. Here,...

journal_title：eLife

authors： Mitchell PS,Roncaioli JL,Turcotte EA,Goers L,Chavez RA,Lee AY,Lesser CF,Rauch I,Vance RE

更新日期：2020-10-19 00:00:00

abstract：:Though neurotransmitters are essential elements in neuronal signal transduction, techniques for in vivo analysis are still limited. Here, we describe an organic electrochemical transistor array (OECT-array) technique for monitoring catecholamine neurotransmitters (CA-NTs) in rat brains. The OECT-array is an active sen...

journal_title：eLife

authors： Xie K,Wang N,Lin X,Wang Z,Zhao X,Fang P,Yue H,Kim J,Luo J,Cui S,Yan F,Shi P

更新日期：2020-02-11 00:00:00

abstract：:The expression of E-Cadherin, a protein best known for its role in cell adhesion, regulates the onset of embryonic differentiation. ...

journal_title：eLife

authors： Sancho M,Rodríguez TA

更新日期：2013-12-17 00:00:00

abstract：:Signaling molecules activate distinct patterns of gene expression to coordinate embryogenesis, but how spatiotemporal expression diversity is generated is an open question. In zebrafish, a BMP signaling gradient patterns the dorsal-ventral axis. We systematically identified target genes responding to BMP and found tha...

journal_title：eLife

authors： Rogers KW,ElGamacy M,Jordan BM,Müller P

更新日期：2020-11-11 00:00:00

abstract：:Baboons, members of the genus Papio, comprise six closely related species distributed throughout sub-Saharan Africa and southwest Arabia. The species exhibit more ecological flexibility and a wider range of social systems than many other primates. This article summarizes our current knowledge of the natural history of...

journal_title：eLife

authors： Fischer J,Higham JP,Alberts SC,Barrett L,Beehner JC,Bergman TJ,Carter AJ,Collins A,Elton S,Fagot J,Ferreira da Silva MJ,Hammerschmidt K,Henzi P,Jolly CJ,Knauf S,Kopp GH,Rogers J,Roos C,Ross C,Seyfarth RM,Silk J,

更新日期：2019-11-12 00:00:00

abstract：:In animal-based biomedical research, both the sex and the age of the animals studied affect disease phenotypes by modifying their susceptibility, presentation and response to treatment. The accurate reporting of experimental methods and materials, including the sex and age of animals, is essential so that other resear...

journal_title：eLife

authors： Flórez-Vargas O,Brass A,Karystianis G,Bramhall M,Stevens R,Cruickshank S,Nenadic G

更新日期：2016-03-03 00:00:00

abstract：:Humans employ different strategies when making decisions. Previous research has reported reduced reliance on model-based strategies with aging, but it remains unclear whether this is due to cognitive or motivational factors. Moreover, it is not clear how aging affects the metacontrol of decision making, that is the dy...

journal_title：eLife

authors： Bolenz F,Kool W,Reiter AM,Eppinger B

更新日期：2019-08-09 00:00:00

abstract：:Actin dynamics generate forces to deform the membrane and overcome the cell's high turgor pressure during clathrin-mediated endocytosis (CME) in yeast, but precise molecular details are still unresolved. Our previous models predicted that actin filaments of the endocytic meshwork continually polymerize and disassemble...

journal_title：eLife

authors： Lacy MM,Baddeley D,Berro J

更新日期：2019-12-19 00:00:00

abstract：:To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we developed a lysine-targeted enrichable cross-linker containing a biotin tag for affinity purification, a chemical cleavage site to separate cross-linked peptides away from biotin after enrichment, and a spacer arm that can be label...

journal_title：eLife

authors： Tan D,Li Q,Zhang MJ,Liu C,Ma C,Zhang P,Ding YH,Fan SB,Tao L,Yang B,Li X,Ma S,Liu J,Feng B,Liu X,Wang HW,He SM,Gao N,Ye K,Dong MQ,Lei X

更新日期：2016-03-08 00:00:00

abstract：:The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...

journal_title：eLife

authors： Lischinsky JE,Sokolowski K,Li P,Esumi S,Kamal Y,Goodrich M,Oboti L,Hammond TR,Krishnamoorthy M,Feldman D,Huntsman M,Liu J,Corbin JG

更新日期：2017-03-13 00:00:00

abstract：:The basal ganglia are known to be involved in the planning, execution and control of gripping force and movement vigour. Here we aim to define the nature of the basal ganglia control signal for force and to decode gripping force based on local field potential (LFP) activities recorded from the subthalamic nucleus (STN...

journal_title：eLife

authors： Tan H,Pogosyan A,Ashkan K,Green AL,Aziz T,Foltynie T,Limousin P,Zrinzo L,Hariz M,Brown P

更新日期：2016-11-18 00:00:00

abstract：:Genome-wide association studies identified the BIN1 locus as a leading modulator of genetic risk in Alzheimer's disease (AD). One limitation in understanding BIN1's contribution to AD is its unknown function in the brain. AD-associated BIN1 variants are generally noncoding and likely change expression. Here, we determ...

journal_title：eLife

authors： Voskobiynyk Y,Roth JR,Cochran JN,Rush T,Carullo NV,Mesina JS,Waqas M,Vollmer RM,Day JJ,McMahon LL,Roberson ED

更新日期：2020-07-13 00:00:00

abstract：:Organisms possessing genetic codes with unassigned codons raise the question of how cellular machinery resolves such codons and how this could impact horizontal gene transfer. Here, we use a genomically recoded Escherichia coli to examine how organisms address translation at unassigned UAG codons, which obstruct propa...

journal_title：eLife

authors： Ma NJ,Hemez CF,Barber KW,Rinehart J,Isaacs FJ

更新日期：2018-10-30 00:00:00