Distinct regulation of dopamine D2S and D2L autoreceptor signaling by calcium.

abstract：:Dephosphorylation of eukaryotic translation initiation factor 2a (eIF2a) restores protein synthesis at the waning of stress responses and requires a PP1 catalytic subunit and a regulatory subunit, PPP1R15A/GADD34 or PPP1R15B/CReP. Surprisingly, PPP1R15-PP1 binary complexes reconstituted in vitro lacked substrate selec...

journal_title：eLife

authors： Chen R,Rato C,Yan Y,Crespillo-Casado A,Clarke HJ,Harding HP,Marciniak SJ,Read RJ,Ron D

更新日期：2015-03-16 00:00:00

abstract：:Hippo signalling integrates diverse stimuli related to epithelial architecture to regulate tissue growth and cell fate decisions. The Hippo kinase cascade represses the growth-promoting transcription co-activator Yorkie. The FERM protein Expanded is one of the main upstream Hippo signalling regulators in Drosophila as...

journal_title：eLife

authors： Fulford AD,Holder MV,Frith D,Snijders AP,Tapon N,Ribeiro PS

更新日期：2019-09-30 00:00:00

abstract：:While the dynamin GTPase Drp1 plays a critical role during mitochondrial fission, mechanisms controlling its recruitment to fission sites are unclear. A current assumption is that cytosolic Drp1 is recruited directly to fission sites immediately prior to fission. Using live-cell microscopy, we find evidence for a diff...

journal_title：eLife

authors： Ji WK,Hatch AL,Merrill RA,Strack S,Higgs HN

更新日期：2015-11-26 00:00:00

abstract：:Journals are exploring new approaches to peer review in order to reduce bias, increase transparency and respond to author preferences. Funders are also getting involved. ...

journal_title：eLife

authors： Rodgers P

更新日期：2017-09-29 00:00:00

abstract：:Presence of multiple copies of the microtubule-binding NDC80 complex is an evolutionary conserved feature of kinetochores, points of attachment of chromosomes to spindle microtubules. This may enable multivalent attachments to microtubules, with implications that remain unexplored. Using recombinant human kinetochore ...

journal_title：eLife

authors： Volkov VA,Huis In 't Veld PJ,Dogterom M,Musacchio A

更新日期：2018-04-09 00:00:00

abstract：:Transient receptor potential (TRP) ion channels in peripheral sensory neurons are functionally regulated by hydrolysis of the phosphoinositide PI(4,5)P2 and changes in the level of protein kinase mediated phosphorylation following activation of various G protein coupled receptors. We now show that the activity of TRPM...

journal_title：eLife

authors： Quallo T,Alkhatib O,Gentry C,Andersson DA,Bevan S

更新日期：2017-08-15 00:00:00

abstract：:Amyloid plaques, consisting of deposited beta-amyloid (Aβ), are a neuropathological hallmark of Alzheimer's Disease (AD). Cerebral vessels play a major role in AD, as Aβ is cleared from the brain by pathways involving the cerebrovasculature, most AD patients have cerebrovascular amyloid (cerebral amyloid angiopathy (C...

journal_title：eLife

authors： Robert J,Button EB,Yuen B,Gilmour M,Kang K,Bahrabadi A,Stukas S,Zhao W,Kulic I,Wellington CL

更新日期：2017-10-10 00:00:00

abstract：:Numerous brain regions have been shown to have neural correlates of gradually accumulating evidence for decision-making, but the causal roles of these regions in decisions driven by accumulation of evidence have yet to be determined. Here, in rats performing an auditory evidence accumulation task, we inactivated the f...

journal_title：eLife

authors： Erlich JC,Brunton BW,Duan CA,Hanks TD,Brody CD

更新日期：2015-04-14 00:00:00

abstract：:Improving the peer review process, overcoming the limitations of print journals and providing open access to the very best work in the life and biomedical sciences are three highlights of our first year. ...

journal_title：eLife

authors： Schekman R,Watt FM,Weigel D

更新日期：2013-10-15 00:00:00

abstract：:Swi2/Snf2 ATPases remodel substrates such as nucleosomes and transcription complexes to control a wide range of DNA-associated processes, but detailed structural information on the ATP-dependent remodeling reactions is largely absent. The single subunit remodeler Mot1 (modifier of transcription 1) dissociates TATA box...

journal_title：eLife

authors： Butryn A,Schuller JM,Stoehr G,Runge-Wollmann P,Förster F,Auble DT,Hopfner KP

更新日期：2015-08-10 00:00:00

abstract：:A strain of budding yeast that contains one large chromosome reveals how the telomere capping complex CST maintains linear but not circular chromosomes. ...

journal_title：eLife

authors： Nugent C,Sugimoto K

更新日期：2020-08-05 00:00:00

abstract：:Tissue elongation and its control by spatiotemporal signals is a major developmental question. Currently, it is thought that Drosophila ovarian follicular epithelium elongation requires the planar polarization of the basal domain cytoskeleton and of the extra-cellular matrix, associated with a dynamic process of rotat...

journal_title：eLife

authors： Alégot H,Pouchin P,Bardot O,Mirouse V

更新日期：2018-02-08 00:00:00

abstract：:Humans can run without falling down, usually despite uneven terrain or occasional pushes. Even without such external perturbations, intrinsic sources like sensorimotor noise perturb the running motion incessantly, making each step variable. Here, using simple and generalizable models, we show that even such small step...

journal_title：eLife

authors： Seethapathi N,Srinivasan M

更新日期：2019-03-19 00:00:00

abstract：:The Maternal Embryonic Leucine Zipper Kinase (MELK) has been identified as a promising therapeutic target in multiple cancer types. MELK over-expression is associated with aggressive disease, and MELK has been implicated in numerous cancer-related processes, including chemotherapy resistance, stem cell renewal, and tu...

journal_title：eLife

authors： Giuliano CJ,Lin A,Smith JC,Palladino AC,Sheltzer JM

更新日期：2018-02-08 00:00:00

abstract：:Mitochondria play important roles in cellular processes and disease, yet little is known about how the transcriptional regime of the mitochondrial genome varies across individuals and tissues. By analyzing >11,000 RNA-sequencing libraries across 36 tissue/cell types, we find considerable variation in mitochondrial-enc...

journal_title：eLife

authors： Ali AT,Boehme L,Carbajosa G,Seitan VC,Small KS,Hodgkinson A

更新日期：2019-02-18 00:00:00

abstract：:Signals delivered by costimulatory molecules are implicated in driving T cell expansion. The requirements for these signals, however, vary from dispensable to essential in different infections. We examined the underlying mechanisms of this differential T cell costimulation dependence and found that the viral context d...

journal_title：eLife

authors： Welten SP,Redeker A,Franken KL,Oduro JD,Ossendorp F,Čičin-Šain L,Melief CJ,Aichele P,Arens R

更新日期：2015-08-11 00:00:00

abstract：:MyD88 is the main adaptor molecule for TLR and IL-1R family members. Here, we demonstrated that T-cell intrinsic MyD88 signaling is required for proliferation, protection from apoptosis and expression of activation/memory genes during infection with the intracellular parasite Trypanosoma cruzi, as evidenced by transcr...

journal_title：eLife

authors： Oliveira AC,Gomes-Neto JF,Barbosa CD,Granato A,Reis BS,Santos BM,Fucs R,Canto FB,Nakaya HI,Nóbrega A,Bellio M

更新日期：2017-09-12 00:00:00

abstract：:Establishing a link between the nonsense-mediated decay pathway and a gene associated with programmed cell death could explain why this pathway is essential in most, but not all, eukaryotes. ...

journal_title：eLife

authors： Saveanu C,Jacquier A

更新日期：2016-04-27 00:00:00

abstract：:Telomerase extends telomere sequences at chromosomal ends to protect genomic DNA. During this process it must select the correct nucleotide from a pool of nucleotides with various sugars and base pairing properties, which is critically important for the proper capping of telomeric sequences by shelterin. Unfortunately...

journal_title：eLife

authors： Schaich MA,Sanford SL,Welfer GA,Johnson SA,Khoang TH,Opresko PL,Freudenthal BD

更新日期：2020-06-05 00:00:00

abstract：:Peptidoglycan (PG) is the main component of bacterial cell walls and the target for many antibiotics. PG biosynthesis is tightly coordinated with cell wall growth and turnover, and many of these control activities depend upon PASTA-domain containing eukaryotic-like serine/threonine protein kinases (PASTA-eSTK) that se...

journal_title：eLife

authors： Wamp S,Rutter ZJ,Rismondo J,Jennings CE,Möller L,Lewis RJ,Halbedel S

更新日期：2020-05-29 00:00:00

abstract：:Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease. Although rigorous efforts identified the presence of 'cancer stem cells (CSCs)' in PDAC and molecular markers for them, stem cell dynamics in vivo have not been clearly demonstrated. Here we focused on Doublecortin-like kinase 1 (Dclk1), known as a CSC ...

journal_title：eLife

authors： Maruno T,Fukuda A,Goto N,Tsuda M,Ikuta K,Hiramatsu Y,Ogawa S,Nakanishi Y,Yamaga Y,Yoshioka T,Takaori K,Uemoto S,Saur D,Chiba T,Seno H

更新日期：2021-01-04 00:00:00

abstract：:Cancer cells usually exhibit aberrant cell signaling and metabolic reprogramming. However, mechanisms of crosstalk between these processes remain elusive. Here, we show that in an in vivo tumor model expressing oncogenic Drosophila Homeodomain-interacting protein kinase (Hipk), tumor cells display elevated aerobic gly...

journal_title：eLife

authors： Wong KKL,Liao JZ,Verheyen EM

更新日期：2019-07-01 00:00:00

abstract：:Conventional Ly6Chi monocytes have developmental plasticity for a spectrum of differentiated phagocytes. Here we show, using conditional deletion strategies in a mouse model of Toll-like receptor (TLR) 7-induced inflammation, that the spectrum of developmental cell fates of Ly6Chi monocytes, and the resultant inflamma...

journal_title：eLife

authors： Gamrekelashvili J,Kapanadze T,Sablotny S,Ratiu C,Dastagir K,Lochner M,Karbach S,Wenzel P,Sitnow A,Fleig S,Sparwasser T,Kalinke U,Holzmann B,Haller H,Limbourg FP

更新日期：2020-07-29 00:00:00

abstract：:Msp1 is a conserved AAA ATPase in budding yeast localized to mitochondria where it prevents accumulation of mistargeted tail-anchored (TA) proteins, including the peroxisomal TA protein Pex15. Msp1 also resides on peroxisomes but it remains unknown how native TA proteins on mitochondria and peroxisomes evade Msp1 surv...

journal_title：eLife

authors： Weir NR,Kamber RA,Martenson JS,Denic V

更新日期：2017-09-14 00:00:00

abstract：:Human patients carrying PAPP-A2 inactivating mutations have low bone mineral density. The underlying mechanisms for this reduced calcification are poorly understood. Using a zebrafish model, we report that Papp-aa regulates bone calcification by promoting Ca2+-transporting epithelial cell (ionocyte) quiescence-prolife...

journal_title：eLife

authors： Liu C,Li S,Noer PR,Kjaer-Sorensen K,Juhl AK,Goldstein A,Ke C,Oxvig C,Duan C

更新日期：2020-04-16 00:00:00

abstract：:Understanding temporal dynamics of COVID-19 symptoms could provide fine-grained resolution to guide clinical decision-making. Here, we use deep neural networks over an institution-wide platform for the augmented curation of clinical notes from 77,167 patients subjected to COVID-19 PCR testing. By contrasting Electroni...

journal_title：eLife

authors： Wagner T,Shweta F,Murugadoss K,Awasthi S,Venkatakrishnan AJ,Bade S,Puranik A,Kang M,Pickering BW,O'Horo JC,Bauer PR,Razonable RR,Vergidis P,Temesgen Z,Rizza S,Mahmood M,Wilson WR,Challener D,Anand P,Liebers M,Doct

更新日期：2020-07-07 00:00:00

abstract：:Establishing how neurocircuit activation causes particular behaviors requires modulating the activity of specific neurons. Here, we demonstrate that magnetothermal genetic stimulation provides tetherless deep brain activation sufficient to evoke motor behavior in awake mice. The approach uses alternating magnetic fiel...

journal_title：eLife

authors： Munshi R,Qadri SM,Zhang Q,Castellanos Rubio I,Del Pino P,Pralle A

更新日期：2017-08-15 00:00:00

abstract：:Neurotransmitter release requires formation of trans-SNARE complexes between the synaptic vesicle and plasma membranes, which likely underlies synaptic vesicle priming to a release-ready state. It is unknown whether Munc18-1, Munc13-1, complexin-1 and synaptotagmin-1 are important for priming because they mediate tran...

journal_title：eLife

authors： Prinslow EA,Stepien KP,Pan YZ,Xu J,Rizo J

更新日期：2019-01-18 00:00:00

abstract：:The Sae2/CtIP protein is required for efficient processing of DNA double-strand breaks that initiate homologous recombination in eukaryotic cells. Sae2/CtIP is also important for survival of single-stranded Top1-induced lesions and CtIP is known to associate directly with transcription-associated complexes in mammalia...

journal_title：eLife

authors： Makharashvili N,Arora S,Yin Y,Fu Q,Wen X,Lee JH,Kao CH,Leung JW,Miller KM,Paull TT

更新日期：2018-12-07 00:00:00

abstract：:We determined whether the mutations found in ovarian cancers could be identified in the patients' ovarian cyst fluids. Tumor-specific mutations were detectable in the cyst fluids of 19 of 23 (83%) borderline tumors, 10 of 13 (77%) type I cancers, and 18 of 18 (100%) type II cancers. In contrast, no mutations were foun...

journal_title：eLife

authors： Wang Y,Sundfeldt K,Mateoiu C,Shih IeM,Kurman RJ,Schaefer J,Silliman N,Kinde I,Springer S,Foote M,Kristjansdottir B,James N,Kinzler KW,Papadopoulos N,Diaz LA,Vogelstein B

更新日期：2016-07-15 00:00:00