Multiple factors maintain assembled trans-SNARE complexes in the presence of NSF and αSNAP.

abstract：:How producers of public goods persist in microbial communities is a major question in evolutionary biology. Cooperation is evolutionarily unstable, since cheating strains can reproduce quicker and take over. Spatial structure has been shown to be a robust mechanism for the evolution of cooperation. Here we study how s...

journal_title：eLife

authors： Uppal G,Vural DC

更新日期：2018-05-22 00:00:00

abstract：:Systemic oxygen restriction (SOR) is prevalent in numerous clinical conditions, including chronic obstructive pulmonary disease (COPD), and is associated with increased susceptibility to viral infections. However, the influence of SOR on T cell immunity remains uncharacterized. Here we show the detrimental effect of h...

journal_title：eLife

authors： Saragovi A,Abramovich I,Omar I,Arbib E,Toker O,Gottlieb E,Berger M

更新日期：2020-11-23 00:00:00

abstract：:Voltage-gated sodium channels are targets for a range of pharmaceutical drugs developed for the treatment of neurological diseases. Cannabidiol (CBD), the non-psychoactive compound isolated from cannabis plants, was recently approved for treatment of two types of epilepsy associated with sodium channel mutations. This...

journal_title：eLife

authors： Sait LG,Sula A,Ghovanloo MR,Hollingworth D,Ruben PC,Wallace BA

更新日期：2020-10-22 00:00:00

abstract：:Strong kinetochore-microtubule attachments are essential for faithful segregation of sister chromatids during mitosis. The Dam1 and Ndc80 complexes are the main microtubule binding components of the Saccharomyces cerevisiae kinetochore. Cooperation between these two complexes enhances kinetochore-microtubule coupling ...

journal_title：eLife

authors： Kim JO,Zelter A,Umbreit NT,Bollozos A,Riffle M,Johnson R,MacCoss MJ,Asbury CL,Davis TN

更新日期：2017-02-13 00:00:00

abstract：:Squamous cell carcinomas occurring at transition zones are highly malignant tumors with poor prognosis. The identity of the cell population and the signaling pathways involved in the progression of transition zone squamous cell carcinoma are poorly understood, hence representing limited options for targeted therapies....

journal_title：eLife

authors： McCauley HA,Chevrier V,Birnbaum D,Guasch G

更新日期：2017-02-21 00:00:00

abstract：:New studies examine how the different sub-structures in the cerebellum are organized to receive information during complex behavioral tasks. ...

journal_title：eLife

authors： Holloway AL,Lerner TN

更新日期：2019-11-14 00:00:00

abstract：:Precise and efficient manipulation of genes is crucial for understanding the molecular mechanisms that govern human hematopoiesis and for developing novel therapies for diseases of the blood and immune system. Current methods do not enable precise engineering of complex genotypes that can be easily tracked in a mixed ...

journal_title：eLife

authors： Bak RO,Dever DP,Reinisch A,Cruz Hernandez D,Majeti R,Porteus MH

更新日期：2017-09-28 00:00:00

abstract：:Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which forms stable membrane-associated nodes at mid-cell. Pom1 gradients rel...

journal_title：eLife

authors： Gerganova V,Floderer C,Archetti A,Michon L,Carlini L,Reichler T,Manley S,Martin SG

更新日期：2019-05-03 00:00:00

abstract：:Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered,...

journal_title：eLife

authors： Song X,Shan H,Zhu Y,Hu S,Xue L,Chen Y,Ding W,Niu T,Gu J,Ouyang S,Shen QT,Liu ZJ

更新日期：2019-07-10 00:00:00

abstract：:Sex-specific synaptic connectivity is beginning to emerge as a remarkable, but little explored feature of animal brains. We describe here a novel mechanism that promotes sexually dimorphic neuronal function and synaptic connectivity in the nervous system of the nematode Caenorhabditis elegans. We demonstrate that a ph...

journal_title：eLife

authors： Bayer EA,Stecky RC,Neal L,Katsamba PS,Ahlsen G,Balaji V,Hoppe T,Shapiro L,Oren-Suissa M,Hobert O

更新日期：2020-10-06 00:00:00

abstract：:Although the wiring of the cerebellar cortex appears to be uniform, the neurons in this region of the brain behave more differently from each other than previously thought. ...

journal_title：eLife

authors： Albergaria C,Carey MR

更新日期：2014-06-10 00:00:00

abstract：:Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remai...

journal_title：eLife

authors： Teves SS,An L,Bhargava-Shah A,Xie L,Darzacq X,Tjian R

更新日期：2018-06-25 00:00:00

abstract：:Genetic techniques have shed new light on the organization of the neurons in the ventral nervous system of the fruit fly. ...

journal_title：eLife

authors： Lovick JK,Omoto JJ,Hartenstein V

更新日期：2015-08-28 00:00:00

abstract：:Myelin, made by oligodendrocytes, is essential for rapid information transfer in the central nervous system. Oligodendrocyte precursors (OPs) receive glutamatergic synaptic input from axons but how this affects their development is unclear. Murine OPs in white matter express AMPA receptor (AMPAR) subunits GluA2, GluA3...

journal_title：eLife

authors： Kougioumtzidou E,Shimizu T,Hamilton NB,Tohyama K,Sprengel R,Monyer H,Attwell D,Richardson WD

更新日期：2017-06-13 00:00:00

abstract：:Nerve tissue contains a high density of chemical synapses, about 1 per µm3 in the mammalian cerebral cortex. Thus, even for small blocks of nerve tissue, dense connectomic mapping requires the identification of millions to billions of synapses. While the focus of connectomic data analysis has been on neurite reconstru...

journal_title：eLife

authors： Staffler B,Berning M,Boergens KM,Gour A,Smagt PV,Helmstaedter M

更新日期：2017-07-14 00:00:00

abstract：:Slo2 potassium channels play important roles in neuronal function, and their mutations in humans may cause epilepsies and cognitive defects. However, it is largely unknown how Slo2 is regulated by other proteins. Here we show that the function of C. elegans Slo2 (SLO-2) depends on adr-1, a gene important to RNA editin...

journal_title：eLife

authors： Niu LG,Liu P,Wang ZW,Chen B

更新日期：2020-04-21 00:00:00

abstract：:Faithful chromosome segregation in all eukaryotes relies on centromeres, the chromosomal sites that recruit kinetochore proteins and mediate spindle attachment during cell division. The centromeric histone H3 variant, CenH3, is the defining chromatin component of centromeres in most eukaryotes, including animals, fung...

journal_title：eLife

authors： Drinnenberg IA,deYoung D,Henikoff S,Malik HS

更新日期：2014-09-23 00:00:00

abstract：:Postmitotic tissues are incapable of replacing damaged cells through proliferation, but need to rely on buffering mechanisms to prevent tissue disintegration. By constitutively activating the Ras/MAPK-pathway via RasV12-overexpression in the postmitotic salivary glands of Drosophila larvae, we overrode the glands adap...

journal_title：eLife

authors： Krautz R,Khalili D,Theopold U

更新日期：2020-12-30 00:00:00

abstract：:In the developing fruit fly brain, a protein called Trithorax increases the number of neural cells produced from a single stem cell, in part by regulating the transcription of the target genes buttonhead and pointed. ...

journal_title：eLife

authors： Jiang Y,Reichert H

更新日期：2014-11-06 00:00:00

abstract：:Cells respond to protein misfolding and aggregation in the cytosol by adjusting gene transcription and a number of post-transcriptional processes. In parallel to functional reactions, cellular structure changes as well; however, the mechanisms underlying the early adaptation of cellular compartments to cytosolic prote...

journal_title：eLife

authors： Kopp Y,Lang WH,Schuster TB,Martínez-Limón A,Hofbauer HF,Ernst R,Calloni G,Vabulas RM

更新日期：2017-11-01 00:00:00

abstract：:We have previously demonstrated that checkpoint kinase 2 (CHK2) is a critical negative regulator of androgen receptor (AR) transcriptional activity, prostate cancer (PCa) cell growth, and androgen sensitivity. We have now uncovered that the AR directly interacts with CHK2 and ionizing radiation (IR) increases this int...

journal_title：eLife

authors： Ta HQ,Dworak N,Ivey ML,Roller DG,Gioeli D

更新日期：2020-06-24 00:00:00

abstract：:Systemic blood pressure is determined, in part, by arterial smooth muscle cells (myocytes). Several Transient Receptor Potential (TRP) channels are proposed to be expressed in arterial myocytes, but it is unclear if these proteins control physiological blood pressure and contribute to hypertension in vivo. We generate...

journal_title：eLife

authors： Bulley S,Fernández-Peña C,Hasan R,Leo MD,Muralidharan P,Mackay CE,Evanson KW,Moreira-Junior L,Mata-Daboin A,Burris SK,Wang Q,Kuruvilla KP,Jaggar JH

更新日期：2018-12-04 00:00:00

abstract：:Transparent reporting is essential for the critical evaluation of studies. However, the reporting of statistical methods for studies in the biomedical sciences is often limited. This systematic review examines the quality of reporting for two statistical tests, t-tests and ANOVA, for papers published in a selection of...

journal_title：eLife

authors： Weissgerber TL,Garcia-Valencia O,Garovic VD,Milic NM,Winham SJ

更新日期：2018-12-21 00:00:00

abstract：:Methyl-coenzyme M reductase (MCR), found in strictly anaerobic methanogenic and methanotrophic archaea, catalyzes the reversible production and consumption of the potent greenhouse gas methane. The α subunit of MCR (McrA) contains several unusual post-translational modifications, including a rare thioamidation of glyc...

journal_title：eLife

authors： Nayak DD,Mahanta N,Mitchell DA,Metcalf WW

更新日期：2017-09-07 00:00:00

abstract：:The Sae2/CtIP protein is required for efficient processing of DNA double-strand breaks that initiate homologous recombination in eukaryotic cells. Sae2/CtIP is also important for survival of single-stranded Top1-induced lesions and CtIP is known to associate directly with transcription-associated complexes in mammalia...

journal_title：eLife

authors： Makharashvili N,Arora S,Yin Y,Fu Q,Wen X,Lee JH,Kao CH,Leung JW,Miller KM,Paull TT

更新日期：2018-12-07 00:00:00

abstract：:In animal-based biomedical research, both the sex and the age of the animals studied affect disease phenotypes by modifying their susceptibility, presentation and response to treatment. The accurate reporting of experimental methods and materials, including the sex and age of animals, is essential so that other resear...

journal_title：eLife

authors： Flórez-Vargas O,Brass A,Karystianis G,Bramhall M,Stevens R,Cruickshank S,Nenadic G

更新日期：2016-03-03 00:00:00

abstract：:A polymorphism in the autophagy gene Atg16l1 is associated with susceptibility to inflammatory bowel disease (IBD); however, it remains unclear how autophagy contributes to intestinal immune homeostasis. Here, we demonstrate that autophagy is essential for maintenance of balanced CD4(+) T cell responses in the intesti...

journal_title：eLife

authors： Kabat AM,Harrison OJ,Riffelmacher T,Moghaddam AE,Pearson CF,Laing A,Abeler-Dörner L,Forman SP,Grencis RK,Sattentau Q,Simon AK,Pott J,Maloy KJ

更新日期：2016-02-24 00:00:00

abstract：:Direct reprogramming of fibroblasts to neurons induces widespread cellular and transcriptional reconfiguration. Here, we characterized global epigenomic changes during the direct reprogramming of mouse fibroblasts to neurons using whole-genome base-resolution DNA methylation (mC) sequencing. We found that the pioneer ...

journal_title：eLife

authors： Luo C,Lee QY,Wapinski O,Castanon R,Nery JR,Mall M,Kareta MS,Cullen SM,Goodell MA,Chang HY,Wernig M,Ecker JR

更新日期：2019-01-15 00:00:00

abstract：:Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactio...

journal_title：eLife

authors： Bawa S,Brooks DS,Neville KE,Tipping M,Sagar MA,Kollhoff JA,Chawla G,Geisbrecht BV,Tennessen JM,Eliceiri KW,Geisbrecht ER

更新日期：2020-03-30 00:00:00

abstract：:Animals host multi-species microbial communities (microbiomes) whose properties may result from inter-species interactions; however, current understanding of host-microbiome interactions derives mostly from studies in which elucidation of microbe-microbe interactions is difficult. In exploring how Drosophila melanogas...

journal_title：eLife

authors： Fischer CN,Trautman EP,Crawford JM,Stabb EV,Handelsman J,Broderick NA

更新日期：2017-01-09 00:00:00