De-repression of the RAC activator ELMO1 in cancer stem cells drives progression of TGFβ-deficient squamous cell carcinoma from transition zones.

abstract：:In the fruit fly optic lobe, T4 and T5 cells represent the first direction-selective neurons, with T4 cells responding selectively to moving brightness increments (ON) and T5 cells to brightness decrements (OFF). Both T4 and T5 cells comprise four subtypes with directional tuning to one of the four cardinal directions...

journal_title：eLife

authors： Haag J,Mishra A,Borst A

更新日期：2017-08-22 00:00:00

abstract：:Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle...

journal_title：eLife

authors： Sun Q,Zhu X,Qi J,An W,Lan P,Tan D,Chen R,Wang B,Zheng S,Zhang C,Chen X,Zhang W,Chen J,Dong MQ,Ye K

更新日期：2017-02-28 00:00:00

abstract：:Two mammalian genes, Kcnt1 and Kcnt2, encode pore-forming subunits of Na(+)-dependent K(+) (KNa) channels. Progress in understanding KNa channels has been hampered by the absence of specific tools and methods for rigorous KNa identification in native cells. Here, we report the genetic disruption of both Kcnt1 and Kcnt...

journal_title：eLife

authors： Martinez-Espinosa PL,Wu J,Yang C,Gonzalez-Perez V,Zhou H,Liang H,Xia XM,Lingle CJ

更新日期：2015-11-11 00:00:00

abstract：:Metazoan development depends on tightly regulated gene expression programs that instruct progenitor cells to adopt specialized fates. Recent work found that posttranslational modifications, such as monoubiquitylation, can determine cell fate also independently of effects on transcription, yet how monoubiquitylation is...

journal_title：eLife

authors： Werner A,Baur R,Teerikorpi N,Kaya DU,Rape M

更新日期：2018-07-12 00:00:00

abstract：:Mechanisms of the neural circuits that guide mating decisions in male flies are becoming clearer. ...

journal_title：eLife

authors： Campetella F,Sachse S

更新日期：2015-12-23 00:00:00

abstract：:During land plant evolution, determinate spore-bearing axes (retained in extant bryophytes such as mosses) were progressively transformed into indeterminate branching shoots with specialized reproductive axes that form flowers. The LEAFY transcription factor, which is required for the first zygotic cell division in mo...

journal_title：eLife

authors： Plackett AR,Conway SJ,Hewett Hazelton KD,Rabbinowitsch EH,Langdale JA,Di Stilio VS

更新日期：2018-10-24 00:00:00

abstract：:Heterogeneity broadly exists in various cell types both during development and at homeostasis. Investigating heterogeneity is crucial for comprehensively understanding the complexity of ontogeny, dynamics, and function of specific cell types. Traditional bulk-labeling techniques are incompetent to dissect heterogeneit...

journal_title：eLife

authors： He S,Tian Y,Feng S,Wu Y,Shen X,Chen K,He Y,Sun Q,Li X,Xu J,Wen Z,Qu JY

更新日期：2020-01-06 00:00:00

abstract：:Msp1 is a conserved AAA ATPase in budding yeast localized to mitochondria where it prevents accumulation of mistargeted tail-anchored (TA) proteins, including the peroxisomal TA protein Pex15. Msp1 also resides on peroxisomes but it remains unknown how native TA proteins on mitochondria and peroxisomes evade Msp1 surv...

journal_title：eLife

authors： Weir NR,Kamber RA,Martenson JS,Denic V

更新日期：2017-09-14 00:00:00

abstract：:hIAPP fibrils are associated with Type-II Diabetes, but the link of hIAPP structure to islet cell death remains elusive. Here we observe that hIAPP fibrils are cytotoxic to cultured pancreatic β-cells, leading us to determine the structure and cytotoxicity of protein segments composing the amyloid spine of hIAPP. Usin...

journal_title：eLife

authors： Krotee P,Rodriguez JA,Sawaya MR,Cascio D,Reyes FE,Shi D,Hattne J,Nannenga BL,Oskarsson ME,Philipp S,Griner S,Jiang L,Glabe CG,Westermark GT,Gonen T,Eisenberg DS

更新日期：2017-01-03 00:00:00

abstract：:Baboons, members of the genus Papio, comprise six closely related species distributed throughout sub-Saharan Africa and southwest Arabia. The species exhibit more ecological flexibility and a wider range of social systems than many other primates. This article summarizes our current knowledge of the natural history of...

journal_title：eLife

authors： Fischer J,Higham JP,Alberts SC,Barrett L,Beehner JC,Bergman TJ,Carter AJ,Collins A,Elton S,Fagot J,Ferreira da Silva MJ,Hammerschmidt K,Henzi P,Jolly CJ,Knauf S,Kopp GH,Rogers J,Roos C,Ross C,Seyfarth RM,Silk J,

更新日期：2019-11-12 00:00:00

abstract：:Modern neuroscience research often requires the coordination of multiple processes such as stimulus generation, real-time experimental control, as well as behavioral and neural measurements. The technical demands required to simultaneously manage these processes with high temporal fidelity is a barrier that limits the...

journal_title：eLife

authors： Kim B,Kenchappa SC,Sunkara A,Chang TY,Thompson L,Doudlah R,Rosenberg A

更新日期：2019-02-07 00:00:00

abstract：:Although mRNAs are localized in the processes of excitatory neurons, it is still unclear whether interneurons also localize a large population of mRNAs. In addition, the variability in the localized mRNA population within and between cell types is unknown. Here we describe the unbiased transcriptomic characterization ...

journal_title：eLife

authors： Perez JD,Dieck ST,Alvarez-Castelao B,Tushev G,Chan IC,Schuman EM

更新日期：2021-01-06 00:00:00

abstract：:Understanding how genes and experience work in concert to generate phenotypic variability will provide a better understanding of individuality. Here, we considered this in the main olfactory epithelium, a chemosensory structure with over a thousand distinct cell types in mice. We identified a subpopulation of olfactor...

journal_title：eLife

authors： Vihani A,Hu XS,Gundala S,Koyama S,Block E,Matsunami H

更新日期：2020-11-24 00:00:00

abstract：:Canonical transient receptor potential channels (TRPC) are involved in receptor-operated and/or store-operated Ca2+ signaling. Inhibition of TRPCs by small molecules was shown to be promising in treating renal diseases. In cells, the channels are regulated by calmodulin (CaM). Molecular details of both CaM and drug bi...

journal_title：eLife

authors： Vinayagam D,Quentin D,Yu-Strzelczyk J,Sitsel O,Merino F,Stabrin M,Hofnagel O,Yu M,Ledeboer MW,Nagel G,Malojcic G,Raunser S

更新日期：2020-11-25 00:00:00

abstract：:Appropriate generalization of learned responses to new situations is vital for adaptive behavior. We provide a circuit-level account of generalization in the electrosensory lobe (ELL) of weakly electric mormyrid fish. Much is already known in this system about a form of learning in which motor corollary discharge sign...

journal_title：eLife

authors： Dempsey C,Abbott LF,Sawtell NB

更新日期：2019-03-14 00:00:00

abstract：:The faithful segregation of eukaryotic chromosomes in mitosis requires that the genome be duplicated completely prior to anaphase. However, cells with large genomes sometimes fail to complete replication during interphase and instead enter mitosis with regions of incompletely replicated DNA. These regions are processe...

journal_title：eLife

authors： Sonneville R,Bhowmick R,Hoffmann S,Mailand N,Hickson ID,Labib K

更新日期：2019-09-23 00:00:00

abstract：:Calcium is a critical second messenger in neurons that contributes to learning and memory, but how the coordination of action potentials of neuronal ensembles with the hippocampal local field potential (LFP) is reflected in dynamic calcium activity remains unclear. Here, we recorded hippocampal calcium activity with e...

journal_title：eLife

authors： Zhou H,Neville KR,Goldstein N,Kabu S,Kausar N,Ye R,Nguyen TT,Gelwan N,Hyman BT,Gomperts SN

更新日期：2019-03-07 00:00:00

abstract：:Kinesin-1 and cytoplasmic dynein are microtubule (MT) motors that transport intracellular cargoes. It remains unclear how these motors move along MTs densely coated with obstacles of various sizes in the cytoplasm. Here, we tested the ability of single and multiple motors to bypass synthetic obstacles on MTs in vitro....

journal_title：eLife

authors： Ferro LS,Can S,Turner MA,ElShenawy MM,Yildiz A

更新日期：2019-09-09 00:00:00

abstract：:The connection between gene loss and the functional adaptation of retained proteins is still poorly understood. We apply phylogenomics and metabolic modeling to detect bacterial species that are evolving by gene loss, with the finding that Actinomycetaceae genomes from human cavities are undergoing sizable reductions,...

journal_title：eLife

authors： Juárez-Vázquez AL,Edirisinghe JN,Verduzco-Castro EA,Michalska K,Wu C,Noda-García L,Babnigg G,Endres M,Medina-Ruíz S,Santoyo-Flores J,Carrillo-Tripp M,Ton-That H,Joachimiak A,Henry CS,Barona-Gómez F

更新日期：2017-03-31 00:00:00

abstract：:Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic t...

journal_title：eLife

authors： Dolan MJ,Frechter S,Bates AS,Dan C,Huoviala P,Roberts RJ,Schlegel P,Dhawan S,Tabano R,Dionne H,Christoforou C,Close K,Sutcliffe B,Giuliani B,Li F,Costa M,Ihrke G,Meissner GW,Bock DD,Aso Y,Rubin GM,Jefferis GS

更新日期：2019-05-21 00:00:00

abstract：:Although respiratory syncytial virus (RSV) is responsible for more human deaths each year than influenza, its pathogenic mechanisms are poorly understood. Here high-resolution quantitative imaging, bioenergetics measurements and mitochondrial membrane potential- and redox-sensitive dyes are used to define RSV's impact...

journal_title：eLife

authors： Hu M,Schulze KE,Ghildyal R,Henstridge DC,Kolanowski JL,New EJ,Hong Y,Hsu AC,Hansbro PM,Wark PA,Bogoyevitch MA,Jans DA

更新日期：2019-06-27 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) present a functional paradox because they lack stable tertiary structure, but nonetheless play a central role in signaling, utilizing a process known as allostery. Historically, allostery in structured proteins has been interpreted in terms of propagated structural changes that...

journal_title：eLife

authors： Li J,White JT,Saavedra H,Wrabl JO,Motlagh HN,Liu K,Sowers J,Schroer TA,Thompson EB,Hilser VJ

更新日期：2017-10-12 00:00:00

abstract：:Sleep affects brain activity globally, but many cortical sleep waves are spatially confined. Local rhythms serve cortical area-specific sleep needs and functions; however, mechanisms controlling locality are unclear. We identify the thalamic reticular nucleus (TRN) as a source for local, sensory-cortex-specific non-ra...

journal_title：eLife

authors： Fernandez LM,Vantomme G,Osorio-Forero A,Cardis R,Béard E,Lüthi A

更新日期：2018-12-25 00:00:00

abstract：:Dietary magnesium (Mg2+) supplementation can enhance memory in young and aged rats. Memory-enhancing capacity was largely ascribed to increases in hippocampal synaptic density and elevated expression of the NR2B subunit of the NMDA-type glutamate receptor. Here we show that Mg2+ feeding also enhances long-term memory ...

journal_title：eLife

authors： Wu Y,Funato Y,Meschi E,Jovanoski KD,Miki H,Waddell S

更新日期：2020-11-26 00:00:00

abstract：:We determined differential gene expression in response to high glucose in lymphoblastoid cell lines derived from matched individuals with type 1 diabetes with and without retinopathy. Those genes exhibiting the largest difference in glucose response were assessed for association with diabetic retinopathy in a genome-w...

journal_title：eLife

authors： Skol AD,Jung SC,Sokovic AM,Chen S,Fazal S,Sosina O,Borkar PP,Lin A,Sverdlov M,Cao D,Swaroop A,Bebu I,DCCT\/EDIC Study group.,Stranger BE,Grassi MA

更新日期：2020-11-09 00:00:00

abstract：:Calcineurin is responsible for mediating a wide variety of cellular processes in response to dynamic calcium (Ca(2+)) signals, yet the precise mechanisms involved in the spatiotemporal control of calcineurin signaling are poorly understood. Here, we use genetically encoded fluorescent biosensors to directly probe the ...

journal_title：eLife

authors： Mehta S,Aye-Han NN,Ganesan A,Oldach L,Gorshkov K,Zhang J

更新日期：2014-07-23 00:00:00

abstract：:SUMO-modification of nuclear proteins has profound effects on gene expression. However, non-toxic chemical tools that modulate sumoylation in cells are lacking. Here, to identify small molecule sumoylation inhibitors we developed a cell-based screen that focused on the well-sumoylated substrate, human Liver Receptor H...

journal_title：eLife

authors： Suzawa M,Miranda DA,Ramos KA,Ang KK,Faivre EJ,Wilson CG,Caboni L,Arkin MR,Kim YS,Fletterick RJ,Diaz A,Schneekloth JS,Ingraham HA

更新日期：2015-12-11 00:00:00

abstract：:Direct reprogramming of fibroblasts to neurons induces widespread cellular and transcriptional reconfiguration. Here, we characterized global epigenomic changes during the direct reprogramming of mouse fibroblasts to neurons using whole-genome base-resolution DNA methylation (mC) sequencing. We found that the pioneer ...

journal_title：eLife

authors： Luo C,Lee QY,Wapinski O,Castanon R,Nery JR,Mall M,Kareta MS,Cullen SM,Goodell MA,Chang HY,Wernig M,Ecker JR

更新日期：2019-01-15 00:00:00

abstract：:Phosphorylation of eIF2α controls translation initiation by restricting the levels of active eIF2-GTP/Met-tRNAi ternary complexes (TC). This modulates the expression of all eukaryotic mRNAs and contributes to the cellular integrated stress response. Key to controlling the activity of eIF2 are translation factors eIF2B...

journal_title：eLife

authors： Jennings MD,Kershaw CJ,Adomavicius T,Pavitt GD

更新日期：2017-03-18 00:00:00

abstract：:The Sae2/CtIP protein is required for efficient processing of DNA double-strand breaks that initiate homologous recombination in eukaryotic cells. Sae2/CtIP is also important for survival of single-stranded Top1-induced lesions and CtIP is known to associate directly with transcription-associated complexes in mammalia...

journal_title：eLife

authors： Makharashvili N,Arora S,Yin Y,Fu Q,Wen X,Lee JH,Kao CH,Leung JW,Miller KM,Paull TT

更新日期：2018-12-07 00:00:00