Actin filaments target the oligomeric maturation of the dynamin GTPase Drp1 to mitochondrial fission sites.

Abstract:

:While the dynamin GTPase Drp1 plays a critical role during mitochondrial fission, mechanisms controlling its recruitment to fission sites are unclear. A current assumption is that cytosolic Drp1 is recruited directly to fission sites immediately prior to fission. Using live-cell microscopy, we find evidence for a different model, progressive maturation of Drp1 oligomers on mitochondria through incorporation of smaller mitochondrially-bound Drp1 units. Maturation of a stable Drp1 oligomer does not forcibly lead to fission. Drp1 oligomers also translocate directionally along mitochondria. Ionomycin, a calcium ionophore, causes rapid mitochondrial accumulation of actin filaments followed by Drp1 accumulation at the fission site, and increases fission rate. Inhibiting actin polymerization, myosin IIA, or the formin INF2 reduces both un-stimulated and ionomycin-induced Drp1 accumulation and mitochondrial fission. Actin filaments bind purified Drp1 and increase GTPase activity in a manner that is synergistic with the mitochondrial protein Mff, suggesting a role for direct Drp1/actin interaction. We propose that Drp1 is in dynamic equilibrium on mitochondria in a fission-independent manner, and that fission factors such as actin filaments target productive oligomerization to fission sites.

journal_name

Elife

journal_title

eLife

authors

Ji WK,Hatch AL,Merrill RA,Strack S,Higgs HN

doi

10.7554/eLife.11553

subject

Has Abstract

pub_date

2015-11-26 00:00:00

pages

e11553

issn

2050-084X

journal_volume

4

pub_type

杂志文章

相关文献

eLife文献大全
  • Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio).

    abstract::The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here, we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.28158

    authors: Cong L,Wang Z,Chai Y,Hang W,Shang C,Yang W,Bai L,Du J,Wang K,Wen Q

    更新日期:2017-09-20 00:00:00

  • Insulin signaling controls neurotransmission via the 4eBP-dependent modification of the exocytotic machinery.

    abstract::Altered insulin signaling has been linked to widespread nervous system dysfunction including cognitive dysfunction, neuropathy and susceptibility to neurodegenerative disease. However, knowledge of the cellular mechanisms underlying the effects of insulin on neuronal function is incomplete. Here, we show that cell aut...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.16807

    authors: Mahoney RE,Azpurua J,Eaton BA

    更新日期:2016-08-15 00:00:00

  • Revisiting the role of Dcc in visual system development with a novel eye clearing method.

    abstract::The Deleted in Colorectal Carcinoma (Dcc) receptor plays a critical role in optic nerve development. Whilst Dcc is expressed postnatally in the eye, its function remains unknown as Dcc knockouts die at birth. To circumvent this drawback, we generated an eye-specific Dcc mutant. To study the organization of the retina ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.51275

    authors: Vigouroux RJ,Cesar Q,Chédotal A,Nguyen-Ba-Charvet KT

    更新日期:2020-02-25 00:00:00

  • The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals.

    abstract::Myosins play essential roles in the development and function of auditory organs and multiple myosin genes are associated with hereditary forms of deafness. Using a forward genetic screen in Drosophila, we identified an E3 ligase, Ubr3, as an essential gene for auditory organ development. Ubr3 negatively regulates the ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.15258

    authors: Li T,Giagtzoglou N,Eberl DF,Jaiswal SN,Cai T,Godt D,Groves AK,Bellen HJ

    更新日期:2016-06-22 00:00:00

  • Small conductance Ca2+-activated K+ channels induce the firing pause periods during the activation of Drosophila nociceptive neurons.

    abstract::In Drosophila larvae, Class IV sensory neurons respond to noxious thermal stimuli and provoke heat avoidance behavior. Previously, we showed that the activated neurons displayed characteristic fluctuations of firing rates, which consisted of repetitive high-frequency spike trains and subsequent pause periods, and we p...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.29754

    authors: Onodera K,Baba S,Murakami A,Uemura T,Usui T

    更新日期:2017-10-16 00:00:00

  • Clearance of beta-amyloid is facilitated by apolipoprotein E and circulating high-density lipoproteins in bioengineered human vessels.

    abstract::Amyloid plaques, consisting of deposited beta-amyloid (Aβ), are a neuropathological hallmark of Alzheimer's Disease (AD). Cerebral vessels play a major role in AD, as Aβ is cleared from the brain by pathways involving the cerebrovasculature, most AD patients have cerebrovascular amyloid (cerebral amyloid angiopathy (C...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.29595

    authors: Robert J,Button EB,Yuen B,Gilmour M,Kang K,Bahrabadi A,Stukas S,Zhao W,Kulic I,Wellington CL

    更新日期:2017-10-10 00:00:00

  • Ready, set, differentiate!

    abstract::The expression of E-Cadherin, a protein best known for its role in cell adhesion, regulates the onset of embryonic differentiation. ...

    journal_title:eLife

    pub_type: 评论,杂志文章

    doi:10.7554/eLife.01839

    authors: Sancho M,Rodríguez TA

    更新日期:2013-12-17 00:00:00

  • Sensitivity and kinetics of signal transmission at the first visual synapse differentially impact visually-guided behavior.

    abstract::In the retina, synaptic transmission between photoreceptors and downstream ON-bipolar neurons (ON-BCs) is mediated by a GPCR pathway, which plays an essential role in vision. However, the mechanisms that control signal transmission at this synapse and its relevance to behavior remain poorly understood. In this study w...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.06358

    authors: Sarria I,Pahlberg J,Cao Y,Kolesnikov AV,Kefalov VJ,Sampath AP,Martemyanov KA

    更新日期:2015-04-16 00:00:00

  • Mutual inhibition among postmitotic neurons regulates robustness of brain wiring in Drosophila.

    abstract::Brain connectivity maps display a delicate balance between individual variation and stereotypy, suggesting the existence of dedicated mechanisms that simultaneously permit and limit individual variation. We show that during the development of the Drosophila central nervous system, mutual inhibition among groups of nei...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.00337

    authors: Langen M,Koch M,Yan J,De Geest N,Erfurth ML,Pfeiffer BD,Schmucker D,Moreau Y,Hassan BA

    更新日期:2013-03-05 00:00:00

  • The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.

    abstract::Mammalian HMG-CoA reductase (HMGCR), the rate-limiting enzyme of the cholesterol biosynthetic pathway and the therapeutic target of statins, is post-transcriptionally regulated by sterol-accelerated degradation. Under cholesterol-replete conditions, HMGCR is ubiquitinated and degraded, but the identity of the E3 ubiqu...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.40009

    authors: Menzies SA,Volkmar N,van den Boomen DJ,Timms RT,Dickson AS,Nathan JA,Lehner PJ

    更新日期:2018-12-13 00:00:00

  • Sex-specific effects of cooperative breeding and colonial nesting on prosociality in corvids.

    abstract::The investigation of prosocial behavior is of particular interest from an evolutionary perspective. Comparisons of prosociality across non-human animal species have, however, so far largely focused on primates, and their interpretation is hampered by the diversity of paradigms and procedures used. Here, we present the...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.58139

    authors: Horn L,Bugnyar T,Griesser M,Hengl M,Izawa EI,Oortwijn T,Rössler C,Scheer C,Schiestl M,Suyama M,Taylor AH,Vanhooland LC,von Bayern AM,Zürcher Y,Massen JJ

    更新日期:2020-10-20 00:00:00

  • TORC2-dependent protein kinase Ypk1 phosphorylates ceramide synthase to stimulate synthesis of complex sphingolipids.

    abstract::Plasma membrane lipid composition must be maintained during growth and under environmental insult. In yeast, signaling mediated by TOR Complex 2 (TORC2)-dependent protein kinase Ypk1 controls lipid abundance and distribution in response to membrane stress. Ypk1, among other actions, alleviates negative regulation of L...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.03779

    authors: Muir A,Ramachandran S,Roelants FM,Timmons G,Thorner J

    更新日期:2014-10-03 00:00:00

  • Time- and polarity-dependent proteomic changes associated with homeostatic scaling at central synapses.

    abstract::In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood. To understand the time-dependent scaling dynamics we treated cultured rat hippocampal cells wi...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.33322

    authors: Schanzenbächer CT,Langer JD,Schuman EM

    更新日期:2018-02-15 00:00:00

  • Evolutionary dynamics of incubation periods.

    abstract::The incubation period for typhoid, polio, measles, leukemia and many other diseases follows a right-skewed, approximately lognormal distribution. Although this pattern was discovered more than sixty years ago, it remains an open question to explain its ubiquity. Here, we propose an explanation based on evolutionary dy...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.30212

    authors: Ottino-Loffler B,Scott JG,Strogatz SH

    更新日期:2017-12-21 00:00:00

  • Regulation of signaling directionality revealed by 3D snapshots of a kinase:regulator complex in action.

    abstract::Two-component systems (TCS) are protein machineries that enable cells to respond to input signals. Histidine kinases (HK) are the sensory component, transferring information toward downstream response regulators (RR). HKs transfer phosphoryl groups to their specific RRs, but also dephosphorylate them, overall ensuring...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.21422

    authors: Trajtenberg F,Imelio JA,Machado MR,Larrieux N,Marti MA,Obal G,Mechaly AE,Buschiazzo A

    更新日期:2016-12-12 00:00:00

  • Loss of Ptpn11 (Shp2) drives satellite cells into quiescence.

    abstract::The equilibrium between proliferation and quiescence of myogenic progenitor and stem cells is tightly regulated to ensure appropriate skeletal muscle growth and repair. The non-receptor tyrosine phosphatase Ptpn11 (Shp2) is an important transducer of growth factor and cytokine signals. Here we combined complex genetic...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.21552

    authors: Griger J,Schneider R,Lahmann I,Schöwel V,Keller C,Spuler S,Nazare M,Birchmeier C

    更新日期:2017-05-02 00:00:00

  • The secrets of success.

    abstract::Imaging sperm as they travel through the female reproductive tract has revealed new details about fertilization at the molecular level. ...

    journal_title:eLife

    pub_type: 评论,杂志文章

    doi:10.7554/eLife.64379

    authors: Komondor KM,Carlson AE

    更新日期:2020-12-02 00:00:00

  • Ageing compromises mouse thymus function and remodels epithelial cell differentiation.

    abstract::Ageing is characterised by cellular senescence, leading to imbalanced tissue maintenance, cell death and compromised organ function. This is first observed in the thymus, the primary lymphoid organ that generates and selects T cells. However, the molecular and cellular mechanisms underpinning these ageing processes re...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.56221

    authors: Baran-Gale J,Morgan MD,Maio S,Dhalla F,Calvo-Asensio I,Deadman ME,Handel AE,Maynard A,Chen S,Green F,Sit RV,Neff NF,Darmanis S,Tan W,May AP,Marioni JC,Ponting CP,Holländer GA

    更新日期:2020-08-25 00:00:00

  • Epimutations are associated with CHROMOMETHYLASE 3-induced de novo DNA methylation.

    abstract::In many plant species, a subset of transcribed genes are characterized by strictly CG-context DNA methylation, referred to as gene body methylation (gbM). The mechanisms that establish gbM are unclear, yet flowering plant species naturally without gbM lack the DNA methyltransferase, CMT3, which maintains CHG (H = A, C...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.47891

    authors: Wendte JM,Zhang Y,Ji L,Shi X,Hazarika RR,Shahryary Y,Johannes F,Schmitz RJ

    更新日期:2019-07-29 00:00:00

  • Long-term potentiation is independent of the C-tail of the GluA1 AMPA receptor subunit.

    abstract::We tested the proposal that the C-terminal domain (CTD) of the AMPAR subunit GluA1 is required for LTP. We found that a knock-in mouse lacking the CTD of GluA1 expresses normal LTP and spatial memory, assayed by the Morris water maze. Our results support a model in which LTP generates synaptic slots, which capture pas...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.58042

    authors: Díaz-Alonso J,Morishita W,Incontro S,Simms J,Holtzman J,Gill M,Mucke L,Malenka RC,Nicoll RA

    更新日期:2020-08-24 00:00:00

  • Cellular aspect ratio and cell division mechanics underlie the patterning of cell progeny in diverse mammalian epithelia.

    abstract::Cell division is essential to expand, shape, and replenish epithelia. In the adult small intestine, cells from a common progenitor intermix with other lineages, whereas cell progeny in many other epithelia form contiguous patches. The mechanisms that generate these distinct patterns of progeny are poorly understood. U...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.36739

    authors: McKinley KL,Stuurman N,Royer LA,Schartner C,Castillo-Azofeifa D,Delling M,Klein OD,Vale RD

    更新日期:2018-06-13 00:00:00

  • Problems on the back of an envelope.

    abstract::Claims that magnetic fields can be used to manipulate biological systems contradict some basic laws of physics. ...

    journal_title:eLife

    pub_type: 评论,杂志文章

    doi:10.7554/eLife.19569

    authors: Anikeeva P,Jasanoff A

    更新日期:2016-09-08 00:00:00

  • Structural organization of a major neuronal G protein regulator, the RGS7-Gβ5-R7BP complex.

    abstract::Signaling by the G-protein-coupled receptors (GPCRs) plays fundamental role in a vast number of essential physiological functions. Precise control of GPCR signaling requires action of regulators of G protein signaling (RGS) proteins that deactivate heterotrimeric G proteins. RGS proteins are elaborately regulated and ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.42150

    authors: Patil DN,Rangarajan ES,Novick SJ,Pascal BD,Kojetin DJ,Griffin PR,Izard T,Martemyanov KA

    更新日期:2018-12-12 00:00:00

  • Temporal dynamics and developmental memory of 3D chromatin architecture at Hox gene loci.

    abstract::Hox genes are essential regulators of embryonic development. Their step-wise transcriptional activation follows their genomic topology and the various states of activation are subsequently memorized into domains of progressively overlapping gene products. We have analyzed the 3D chromatin organization of Hox clusters ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.02557

    authors: Noordermeer D,Leleu M,Schorderet P,Joye E,Chabaud F,Duboule D

    更新日期:2014-04-29 00:00:00

  • Bugs battle stress from hot blood.

    abstract::A heat exchange mechanism in the head of kissing bugs helps to prevent stress and regulate their temperature while they feed on warm blood. ...

    journal_title:eLife

    pub_type: 评论,杂志文章

    doi:10.7554/eLife.33035

    authors: Benoit JB,Denlinger DL

    更新日期:2017-11-21 00:00:00

  • Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages.

    abstract::Macrophages are highly plastic cells with critical roles in immunity, cancer, and tissue homeostasis, but how these distinct cellular fates are triggered by environmental cues is poorly understood. To uncover how primary murine macrophages respond to bacterial pathogens, we globally assessed changes in post-translatio...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.51461

    authors: Budzik JM,Swaney DL,Jimenez-Morales D,Johnson JR,Garelis NE,Repasy T,Roberts AW,Popov LM,Parry TJ,Pratt D,Ideker T,Krogan NJ,Cox JS

    更新日期:2020-01-17 00:00:00

  • Mature oligodendrocytes bordering lesions limit demyelination and favor myelin repair via heparan sulfate production.

    abstract::Myelin destruction is followed by resident glia activation and mobilization of endogenous progenitors (OPC) which participate in myelin repair. Here we show that in response to demyelination, mature oligodendrocytes (OLG) bordering the lesion express Ndst1, a key enzyme for heparan sulfates (HS) synthesis. Ndst1+ OLG ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.51735

    authors: Macchi M,Magalon K,Zimmer C,Peeva E,El Waly B,Brousse B,Jaekel S,Grobe K,Kiefer F,Williams A,Cayre M,Durbec P

    更新日期:2020-06-09 00:00:00

  • Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse.

    abstract::Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans after silicone oil (S...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.45881

    authors: Zhang J,Li L,Huang H,Fang F,Webber HC,Zhuang P,Liu L,Dalal R,Tang PH,Mahajan VB,Sun Y,Li S,Zhang M,Goldberg JL,Hu Y

    更新日期:2019-05-15 00:00:00

  • The single-cell eQTLGen consortium.

    abstract::In recent years, functional genomics approaches combining genetic information with bulk RNA-sequencing data have identified the downstream expression effects of disease-associated genetic risk factors through so-called expression quantitative trait locus (eQTL) analysis. Single-cell RNA-sequencing creates enormous opp...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.52155

    authors: van der Wijst M,de Vries DH,Groot HE,Trynka G,Hon CC,Bonder MJ,Stegle O,Nawijn MC,Idaghdour Y,van der Harst P,Ye CJ,Powell J,Theis FJ,Mahfouz A,Heinig M,Franke L

    更新日期:2020-03-09 00:00:00

  • Supervised mutational signatures for obesity and other tissue-specific etiological factors in cancer.

    abstract::Determining the etiologic basis of the mutations that are responsible for cancer is one of the fundamental challenges in modern cancer research. Different mutational processes induce different types of DNA mutations, providing 'mutational signatures' that have led to key insights into cancer etiology. The most widely ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.61082

    authors: Afsari B,Kuo A,Zhang Y,Li L,Lahouel K,Danilova L,Favorov A,Rosenquist TA,Grollman AP,Kinzler KW,Cope L,Vogelstein B,Tomasetti C

    更新日期:2021-01-25 00:00:00