Abstract:
:Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease. Although rigorous efforts identified the presence of 'cancer stem cells (CSCs)' in PDAC and molecular markers for them, stem cell dynamics in vivo have not been clearly demonstrated. Here we focused on Doublecortin-like kinase 1 (Dclk1), known as a CSC marker of PDAC. Using genetic lineage tracing with a dual-recombinase system and live imaging, we showed that Dclk1+ tumor cells continuously provided progeny cells within pancreatic intraepithelial neoplasia, primary and metastatic PDAC, and PDAC-derived spheroids in vivo and in vitro. Furthermore, genes associated with CSC and epithelial mesenchymal transition were enriched in mouse Dclk1+ and human DCLK1-high PDAC cells. Thus, we provided direct functional evidence for the stem cell activity of Dclk1+ cells in vivo, revealing the essential roles of Dclk1+ cells in expansion of pancreatic neoplasia in all progressive stages.
journal_name
Elifejournal_title
eLifeauthors
Maruno T,Fukuda A,Goto N,Tsuda M,Ikuta K,Hiramatsu Y,Ogawa S,Nakanishi Y,Yamaga Y,Yoshioka T,Takaori K,Uemoto S,Saur D,Chiba T,Seno Hdoi
10.7554/eLife.55117subject
Has Abstractpub_date
2021-01-04 00:00:00issn
2050-084Xpii
55117journal_volume
10pub_type
杂志文章相关文献
eLife文献大全abstract::Fruit fly neuroblasts can either self-renew, rest or take on a specialized form, depending on the levels of a protein called Prospero. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04869
更新日期:2014-10-29 00:00:00
abstract::Actin filaments assemble inside the nucleus in response to multiple cellular perturbations, including heat shock, protein misfolding, integrin engagement, and serum stimulation. We find that DNA damage also generates nuclear actin filaments-detectable by phalloidin and live-cell actin probes-with three characteristic ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07735
更新日期:2015-08-19 00:00:00
abstract::Many species, including rats, are sensitive to social signals and their valuation is important in social learning. Here we introduce a task that investigates if mutual reward delivery in male rats can drive associative learning. We found that when actor rats have fully learned a stimulus-self-reward association, addin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60755
更新日期:2020-10-01 00:00:00
abstract::During translation elongation, the ribosome ratchets along its mRNA template, incorporating each new amino acid and translocating from one codon to the next. The elongation cycle requires dramatic structural rearrangements of the ribosome. We show here that deep sequencing of ribosome-protected mRNA fragments reveals ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01257
更新日期:2014-05-09 00:00:00
abstract::Determining the etiologic basis of the mutations that are responsible for cancer is one of the fundamental challenges in modern cancer research. Different mutational processes induce different types of DNA mutations, providing 'mutational signatures' that have led to key insights into cancer etiology. The most widely ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.61082
更新日期:2021-01-25 00:00:00
abstract::Cerebral cortical architecture at birth encodes regionally differential dendritic arborization and synaptic formation. It underlies behavioral emergence of 2-year-olds. Brain changes in 0-2 years are most dynamic across the lifespan. Effective prediction of future behavior with brain microstructure at birth will revea...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58116
更新日期:2020-12-22 00:00:00
abstract::Beige/brite adipocytes are induced within white adipose tissues (WAT) and, when activated, consume glucose and fatty acids to produce heat. Classically, two stimuli have been used to trigger a beiging response: cold temperatures and β3-adrenergic receptor (Adrb3) agonists. These two beiging triggers have been used int...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30329
更新日期:2017-10-11 00:00:00
abstract::Lipoprotein lipase (LPL) undergoes spontaneous inactivation via global unfolding and this unfolding is prevented by GPIHBP1 (Mysling et al., 2016). We now show: (1) that ANGPTL4 inactivates LPL by catalyzing the unfolding of its hydrolase domain; (2) that binding to GPIHBP1 renders LPL largely refractory to this inhib...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20958
更新日期:2016-12-08 00:00:00
abstract::Unfolded protein responses (UPRs) safeguard cellular function during proteotoxic stress and aging. In a previous paper (Lehrbach and Ruvkun, 2016) we showed that the ER-associated SKN-1A/Nrf1 transcription factor activates proteasome subunit expression in response to proteasome dysfunction, but it was not established ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44425
更新日期:2019-04-11 00:00:00
abstract::Establishing a link between the nonsense-mediated decay pathway and a gene associated with programmed cell death could explain why this pathway is essential in most, but not all, eukaryotes. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.16076
更新日期:2016-04-27 00:00:00
abstract::Transposable elements (TEs), the movement of which can damage the genome, are epigenetically silenced in eukaryotes. Intriguingly, TEs are activated in the sperm companion cell - vegetative cell (VC) - of the flowering plant Arabidopsis thaliana. However, the extent and mechanism of this activation are unknown. Here w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.42530
更新日期:2019-05-28 00:00:00
abstract::The Escherichia coli DNA replication machinery has been used as a road map to uncover design rules that enable DNA duplication with high efficiency and fidelity. Although the enzymatic activities of the replicative DNA Pol III are well understood, its dynamics within the replisome are not. Here, we test the accepted v...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23932
更新日期:2017-04-22 00:00:00
abstract::Heterogeneity broadly exists in various cell types both during development and at homeostasis. Investigating heterogeneity is crucial for comprehensively understanding the complexity of ontogeny, dynamics, and function of specific cell types. Traditional bulk-labeling techniques are incompetent to dissect heterogeneit...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52024
更新日期:2020-01-06 00:00:00
abstract::Apical constriction is a widely utilized cell shape change linked to folding, bending and invagination of polarized epithelia. It remains unclear how apical constriction is regulated spatiotemporally during tissue invagination and how this cellular process contributes to tube formation in different developmental conte...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22235
更新日期:2017-03-06 00:00:00
abstract::Amyloid plaques, consisting of deposited beta-amyloid (Aβ), are a neuropathological hallmark of Alzheimer's Disease (AD). Cerebral vessels play a major role in AD, as Aβ is cleared from the brain by pathways involving the cerebrovasculature, most AD patients have cerebrovascular amyloid (cerebral amyloid angiopathy (C...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.29595
更新日期:2017-10-10 00:00:00
abstract::Kinesin super family protein 2A (KIF2A), an ATP-dependent microtubule (MT) destabilizer, regulates cell migration, axon elongation, and pruning in the developing nervous system. KIF2A mutations have recently been identified in patients with malformed cortical development. However, postnatal KIF2A is continuously expre...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30935
更新日期:2018-01-09 00:00:00
abstract::We collated contact tracing data from COVID-19 clusters in Singapore and Tianjin, China and estimated the extent of pre-symptomatic transmission by estimating incubation periods and serial intervals. The mean incubation periods accounting for intermediate cases were 4.91 days (95%CI 4.35, 5.69) and 7.54 (95%CI 6.76, 8...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57149
更新日期:2020-06-22 00:00:00
abstract::Numerous links exist between co-transcriptional RNA processing and the transcribing RNAPII. In particular, pre-mRNA splicing was reported to be associated with slowed RNAPII elongation. Here, we identify a site of ubiquitination (K1246) in the catalytic subunit of RNAPII close to the DNA entry path. Ubiquitination was...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27082
更新日期:2017-10-13 00:00:00
abstract::Current models successfully describe the auditory cortical response to natural sounds with a set of spectro-temporal features. However, these models have hardly been linked to the ill-understood neurobiological changes that occur in the aging auditory cortex. Modelling the hemodynamic response to a rich natural sound ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.55300
更新日期:2020-07-03 00:00:00
abstract::Perturbation of neuronal activity is key to understanding the brain's functional properties, however, intervention studies typically perturb neurons in a nonspecific manner. Recent optogenetics techniques have enabled patterned perturbations, in which specific patterns of activity can be invoked in identified target n...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52757
更新日期:2020-02-19 00:00:00
abstract::Zebra finches communicate with each other in ways that are more complex than previously thought. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11665
更新日期:2015-10-21 00:00:00
abstract::Being exposed to cannabinoids in the womb has different consequences for male and female rats. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.41229
更新日期:2018-09-28 00:00:00
abstract::GPIHBP1, a GPI-anchored protein of capillary endothelial cells, binds lipoprotein lipase (LPL) within the subendothelial spaces and shuttles it to the capillary lumen. GPIHBP1-bound LPL is essential for the margination of triglyceride-rich lipoproteins (TRLs) along capillaries, allowing the lipolytic processing of TRL...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47178
更新日期:2019-06-06 00:00:00
abstract::How circuits assemble starting from stem cells is a fundamental question in developmental neurobiology. We test the hypothesis that, in neuronal stem cells, temporal transcription factors predictably control neuronal terminal features and circuit assembly. Using the Drosophila motor system, we manipulate expression of...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46089
更新日期:2019-09-10 00:00:00
abstract::Brief periods of sleep loss have long-lasting consequences such as impaired memory consolidation. Structural changes in synaptic connectivity have been proposed as a substrate of memory storage. Here, we examine the impact of brief periods of sleep deprivation on dendritic structure. In mice, we find that five hours o...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.13424
更新日期:2016-08-23 00:00:00
abstract::Dietary magnesium (Mg2+) supplementation can enhance memory in young and aged rats. Memory-enhancing capacity was largely ascribed to increases in hippocampal synaptic density and elevated expression of the NR2B subunit of the NMDA-type glutamate receptor. Here we show that Mg2+ feeding also enhances long-term memory ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.61339
更新日期:2020-11-26 00:00:00
abstract::Human patients carrying PAPP-A2 inactivating mutations have low bone mineral density. The underlying mechanisms for this reduced calcification are poorly understood. Using a zebrafish model, we report that Papp-aa regulates bone calcification by promoting Ca2+-transporting epithelial cell (ionocyte) quiescence-prolife...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52322
更新日期:2020-04-16 00:00:00
abstract::The spinal cord has the capacity to coordinate motor activities such as locomotion. Following spinal transection, functional activity can be regained, to a degree, following motor training. To identify microcircuits involved in this recovery, we studied a population of mouse spinal interneurons known to receive direct...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21715
更新日期:2016-12-15 00:00:00
abstract::Phenazines are natural bacterial antibiotics that can protect crops from disease. However, for most crops it is unknown which producers and specific phenazines are ecologically relevant, and whether phenazine biodegradation can counter their effects. To better understand their ecology, we developed and environmentally...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59726
更新日期:2020-09-15 00:00:00
abstract::Altered insulin signaling has been linked to widespread nervous system dysfunction including cognitive dysfunction, neuropathy and susceptibility to neurodegenerative disease. However, knowledge of the cellular mechanisms underlying the effects of insulin on neuronal function is incomplete. Here, we show that cell aut...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16807
更新日期:2016-08-15 00:00:00