The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment.

abstract：:Genetic techniques have shed new light on the organization of the neurons in the ventral nervous system of the fruit fly. ...

journal_title：eLife

authors： Lovick JK,Omoto JJ,Hartenstein V

更新日期：2015-08-28 00:00:00

abstract：:Synaptic positions underlie precise circuit connectivity. Synaptic positions can be established during embryogenesis and sustained during growth. The mechanisms that sustain synaptic specificity during allometric growth are largely unknown. We performed forward genetic screens in C. elegans for regulators of this proc...

journal_title：eLife

authors： Fan J,Ji T,Wang K,Huang J,Wang M,Manning L,Dong X,Shi Y,Zhang X,Shao Z,Colón-Ramos DA

更新日期：2020-04-07 00:00:00

abstract：:This study examined records of 2566 consecutive COVID-19 patients at five Massachusetts hospitals and sought to predict level-of-care requirements based on clinical and laboratory data. Several classification methods were applied and compared against standard pneumonia severity scores. The need for hospitalization, IC...

journal_title：eLife

authors： Hao B,Sotudian S,Wang T,Xu T,Hu Y,Gaitanidis A,Breen K,Velmahos GC,Paschalidis IC

更新日期：2020-10-12 00:00:00

abstract：:Recently, a small-molecule communication mechanism was discovered in a range of Bacillus-infecting bacteriophages, which these temperate phages use to inform their lysis-lysogeny decision. We present a mathematical model of the ecological and evolutionary dynamics of such viral communication, and show that a communica...

journal_title：eLife

authors： Doekes HM,Mulder GA,Hermsen R

更新日期：2021-01-18 00:00:00

abstract：:Vesicle fusion is mediated by an assembly of SNARE proteins between opposing membranes, but it is unknown whether transmembrane domains (TMDs) of SNARE proteins serve mechanistic functions that go beyond passive anchoring of the force-generating SNAREpin to the fusing membranes. Here, we show that conformational flexi...

journal_title：eLife

authors： Dhara M,Yarzagaray A,Makke M,Schindeldecker B,Schwarz Y,Shaaban A,Sharma S,Böckmann RA,Lindau M,Mohrmann R,Bruns D

更新日期：2016-06-25 00:00:00

abstract：:The mechanisms underlying axon regeneration in mature neurons are relevant to the understanding of normal nervous system maintenance and for developing therapeutic strategies for injury. Here, we report novel pathways in axon regeneration, identified by extending our previous function-based screen using the C. elegans...

journal_title：eLife

authors： Kim KW,Tang NH,Piggott CA,Andrusiak MG,Park S,Zhu M,Kurup N,Cherra SJ 3rd,Wu Z,Chisholm AD,Jin Y

更新日期：2018-11-21 00:00:00

abstract：:Replication-dependent (RD) core histone mRNA produced during S-phase is the only known metazoan protein-coding mRNA presenting a 3' stem-loop instead of the otherwise universal polyA tail. A metallo β-lactamase (MBL) fold enzyme, cleavage and polyadenylation specificity factor 73 (CPSF73), is proposed to be the sole e...

journal_title：eLife

authors： Pettinati I,Grzechnik P,Ribeiro de Almeida C,Brem J,McDonough MA,Dhir S,Proudfoot NJ,Schofield CJ

更新日期：2018-12-03 00:00:00

abstract：:TMEM16A is a ligand-gated anion channel that is activated by intracellular Ca2+. This channel comprises two independent pores and closely apposed Ca2+ binding sites that are contained within each subunit of a homodimeric protein. Previously we characterized the influence of positively charged pore-lining residues on a...

journal_title：eLife

authors： Lam AK,Dutzler R

更新日期：2018-10-12 00:00:00

abstract：:Rising and more variable global temperatures pose a challenge for biodiversity, with reproduction and fertility being especially sensitive to heat. Here, we assessed the potential for thermal adaptation in sperm and egg function using Tribolium flour beetles, a warm-temperate-tropical insect model. Following temperatu...

journal_title：eLife

authors： Vasudeva R,Sutter A,Sales K,Dickinson ME,Lumley AJ,Gage MJ

更新日期：2019-10-01 00:00:00

abstract：:White adipose tissue (WAT) inflammation contributes to the development of insulin resistance in obesity. While the role of adipose tissue macrophage (ATM) pro-inflammatory signalling in the development of insulin resistance has been established, it is less clear how WAT inflammation is initiated. Here, we show that AT...

journal_title：eLife

authors： Petkevicius K,Virtue S,Bidault G,Jenkins B,Çubuk C,Morgantini C,Aouadi M,Dopazo J,Serlie MJ,Koulman A,Vidal-Puig A

更新日期：2019-08-16 00:00:00

abstract：:Mitochondria play important roles in cellular processes and disease, yet little is known about how the transcriptional regime of the mitochondrial genome varies across individuals and tissues. By analyzing >11,000 RNA-sequencing libraries across 36 tissue/cell types, we find considerable variation in mitochondrial-enc...

journal_title：eLife

authors： Ali AT,Boehme L,Carbajosa G,Seitan VC,Small KS,Hodgkinson A

更新日期：2019-02-18 00:00:00

abstract：:How different helicase families with a conserved catalytic 'helicase core' evolved to function on varied RNA and DNA substrates by diverse mechanisms remains unclear. In this study, we used Mss116, a yeast DEAD-box protein that utilizes ATP to locally unwind dsRNA, to investigate helicase specificity and mechanism. Ou...

journal_title：eLife

authors： Mallam AL,Sidote DJ,Lambowitz AM

更新日期：2014-12-12 00:00:00

abstract：:A key hallmark of visual perceptual awareness is robustness to instabilities arising from unnoticeable eye and eyelid movements. In previous human intracranial (iEEG) work (Golan et al., 2016) we found that excitatory broadband high-frequency activity transients, driven by eye blinks, are suppressed in higher-level bu...

journal_title：eLife

authors： Golan T,Davidesco I,Meshulam M,Groppe DM,Mégevand P,Yeagle EM,Goldfinger MS,Harel M,Melloni L,Schroeder CE,Deouell LY,Mehta AD,Malach R

更新日期：2017-08-29 00:00:00

abstract：:Pervasive expression of circular RNA is a recently discovered feature of eukaryotic gene expression programs, yet its function remains largely unknown. The presumed biogenesis of these RNAs involves a non-canonical 'backsplicing' event. Recent studies in mammalian cell culture posit that backsplicing is facilitated by...

journal_title：eLife

authors： Barrett SP,Wang PL,Salzman J

更新日期：2015-06-09 00:00:00

abstract：:Neurons coding for head-direction are crucial for spatial navigation. Here we explored the cellular basis of head-direction coding in the rat dorsal presubiculum (PreS). We found that layer2 is composed of two principal cell populations (calbindin-positive and calbindin-negative neurons) which targeted the contralater...

journal_title：eLife

authors： Preston-Ferrer P,Coletta S,Frey M,Burgalossi A

更新日期：2016-06-10 00:00:00

abstract：:The orbitofrontal cortex (OFC) is critical for updating reward-directed behaviours flexibly when outcomes are devalued or when task contingencies are reversed. Failure to update behaviour in outcome devaluation and reversal learning procedures are considered canonical deficits following OFC lesions in non-human primat...

journal_title：eLife

authors： Panayi MC,Killcross S

更新日期：2018-07-25 00:00:00

abstract：:The neural correlates of consciousness are typically sought by comparing the overall brain responses to perceived and unperceived stimuli. However, this comparison may be contaminated by non-specific attention, alerting, performance, and reporting confounds. Here, we pursue a novel approach, tracking the neuronal codi...

journal_title：eLife

authors： Salti M,Monto S,Charles L,King JR,Parkkonen L,Dehaene S

更新日期：2015-05-21 00:00:00

abstract：:The clathrin adaptor complex AP2 is thought to be an obligate heterotetramer. We identify null mutations in the α subunit of AP2 in the nematode Caenorhabditis elegans. α-adaptin mutants are viable and the remaining μ2/β hemicomplex retains some function. Conversely, in μ2 mutants, the alpha/sigma2 hemicomplex is loca...

journal_title：eLife

authors： Gu M,Liu Q,Watanabe S,Sun L,Hollopeter G,Grant BD,Jorgensen EM

更新日期：2013-03-05 00:00:00

abstract：:Pioneer factors such as Zelda (Zld) help initiate zygotic transcription in Drosophila early embryos, but whether other factors support this dynamic process is unclear. Odd-paired (Opa), a zinc-finger transcription factor expressed at cellularization, controls the transition of genes from pair-rule to segmental pattern...

journal_title：eLife

authors： Koromila T,Gao F,Iwasaki Y,He P,Pachter L,Gergen JP,Stathopoulos A

更新日期：2020-07-23 00:00:00

abstract：:A mathematical model shows how the shape of early multicellular organisms may have helped cells evolve specialized roles. ...

journal_title：eLife

authors： Staps M,Tarnita C

更新日期：2020-11-03 00:00:00

abstract：:The receptor protein Dectin-1 recognizes structures found on cancerous cells, and then triggers an anti-tumor immune response. ...

journal_title：eLife

authors： Okabe Y,Medzhitov R

更新日期：2014-09-24 00:00:00

abstract：:Humans and other animals base important decisions on estimates of number, and intraparietal cortex is thought to provide a crucial substrate of this ability. However, it remains debated whether an independent neuronal processing mechanism underlies this 'number sense', or whether number is instead judged indirectly on...

journal_title：eLife

authors： Castaldi E,Piazza M,Dehaene S,Vignaud A,Eger E

更新日期：2019-07-24 00:00:00

abstract：:Although several sleep-regulating neuronal populations have been identified, little is known about how they interact with each other to control sleep/wake states. We previously identified neuropeptide VF (NPVF) and the hypothalamic neurons that produce it as a sleep-promoting system (Lee et al., 2017). Here we show us...

journal_title：eLife

authors： Lee DA,Oikonomou G,Cammidge T,Andreev A,Hong Y,Hurley H,Prober DA

更新日期：2020-12-18 00:00:00

abstract：:Temporary circuits amplify spontaneous activity in the visual system of neonatal rats. ...

journal_title：eLife

authors： Fogerson PM,Huguenard JR

更新日期：2016-10-11 00:00:00

abstract：:Meibum lipids form a lipid layer on the outermost side of the tear film and function to prevent water evaporation and reduce surface tension. (O-Acyl)-ω-hydroxy fatty acids (OAHFAs), a subclass of these lipids, are thought to be involved in connecting the lipid and aqueous layers in tears, although their actual functi...

journal_title：eLife

authors： Miyamoto M,Sassa T,Sawai M,Kihara A

更新日期：2020-04-07 00:00:00

abstract：:Fruit fly neuroblasts can either self-renew, rest or take on a specialized form, depending on the levels of a protein called Prospero. ...

journal_title：eLife

authors： Pollarolo G,Gonzalez C

更新日期：2014-10-29 00:00:00

abstract：:Group B Streptococcus (GBS) is the leading cause of invasive bacterial neonatal infections. Late-onset diseases (LOD) occur between 7 and 89 days of life and are largely due to the CC17 GBS hypervirulent clone. We studied the impact of estradiol (E2) and progesterone (P4), which impregnate the fetus during pregnancy, ...

journal_title：eLife

authors： Hays C,Touak G,Bouaboud A,Fouet A,Guignot J,Poyart C,Tazi A

更新日期：2019-11-11 00:00:00

abstract：:In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches ...

journal_title：eLife

authors： Yin Y,Dominska M,Yim E,Petes TD

更新日期：2017-07-17 00:00:00

abstract：:The pressure for every research article to tell a clear story often leads researchers in the life sciences to exclude experiments that 'did not work' when they write up their results. However, this practice can lead to reporting bias if the decisions about which experiments to exclude are taken after data have been co...

journal_title：eLife

authors： Neves K,Amaral OB

更新日期：2020-05-22 00:00:00

abstract：:Experience-dependent modifications of synaptic connections are thought to change patterns of network activities and stimulus tuning with learning. However, only a few studies explored how synaptic plasticity shapes the response dynamics of cortical circuits. Here, we investigated the mechanism underlying sharpening of...

journal_title：eLife

authors： Lim S

更新日期：2019-08-08 00:00:00