Abstract:
:Pioneer factors such as Zelda (Zld) help initiate zygotic transcription in Drosophila early embryos, but whether other factors support this dynamic process is unclear. Odd-paired (Opa), a zinc-finger transcription factor expressed at cellularization, controls the transition of genes from pair-rule to segmental patterns along the anterior-posterior axis. Finding that Opa also regulates expression through enhancer sog_Distal along the dorso-ventral axis, we hypothesized Opa's role is more general. Chromatin-immunoprecipitation (ChIP-seq) confirmed its in vivo binding to sog_Distal but also identified widespread binding throughout the genome, comparable to Zld. Furthermore, chromatin assays (ATAC-seq) demonstrate that Opa, like Zld, influences chromatin accessibility genome-wide at cellularization, suggesting both are pioneer factors with common as well as distinct targets. Lastly, embryos lacking opa exhibit widespread, late patterning defects spanning both axes. Collectively, these data suggest Opa is a general timing factor and likely late-acting pioneer factor that drives a secondary wave of zygotic gene expression.
journal_name
Elifejournal_title
eLifeauthors
Koromila T,Gao F,Iwasaki Y,He P,Pachter L,Gergen JP,Stathopoulos Adoi
10.7554/eLife.59610subject
Has Abstractpub_date
2020-07-23 00:00:00issn
2050-084Xpii
59610journal_volume
9pub_type
评论,杂志文章相关文献
eLife文献大全abstract::Post-transcriptional control by small regulatory RNA (sRNA) is critical for rapid adaptive processes. sRNAs can directly modulate mRNA degradation in Proteobacteria without interfering with translation. However, Firmicutes have a fundamentally different set of ribonucleases for mRNA degradation and whether sRNAs can r...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23602
更新日期:2017-04-24 00:00:00
abstract::The enteric nervous system controls a variety of gastrointestinal functions including intestinal motility. The minimal neuronal circuit necessary to direct peristalsis is well-characterized but several intestinal regions display also other motility patterns for which the underlying circuits and connectivity schemes th...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.42914
更新日期:2019-02-12 00:00:00
abstract::We present a reanalysis of the stochastic model of organelle production and show that the equilibrium distributions for the organelle numbers predicted by this model can be readily calculated in three different scenarios. These three distributions can be identified as standard distributions, and the corresponding exac...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10167
更新日期:2015-12-22 00:00:00
abstract::The NuRD complex subunit CHD4 is essential for fusion-positive rhabdomyosarcoma (FP-RMS) survival, but the mechanisms underlying this dependency are not understood. Here, a NuRD-specific CRISPR screen demonstrates that FP-RMS is particularly sensitive to CHD4 amongst the NuRD members. Mechanistically, NuRD complex con...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54993
更新日期:2020-08-03 00:00:00
abstract::In humans, large conductance voltage- and calcium-dependent potassium (BK) channels are regulated allosterically by transmembrane voltage and intracellular Ca2+. Divalent cation binding sites reside within the gating ring formed by two Regulator of Conductance of Potassium (RCK) domains per subunit. Using patch-clamp ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40664
更新日期:2018-12-11 00:00:00
abstract::New studies examine how the different sub-structures in the cerebellum are organized to receive information during complex behavioral tasks. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.52631
更新日期:2019-11-14 00:00:00
abstract::We reveal a novel mechanism that explains how preparatory activity can evolve in motor-related cortical areas without prematurely inducing movement. The smooth eye movement region of the frontal eye fields (FEFSEM) is a critical node in the neural circuit controlling smooth pursuit eye movement. Preparatory activity e...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50962
更新日期:2020-02-21 00:00:00
abstract::Neural circuits that control copulation in male flies have been identified. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.22215
更新日期:2016-11-15 00:00:00
abstract::Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22086
更新日期:2017-02-28 00:00:00
abstract::Direct activation of the human phospholipase C-γ isozymes (PLC-γ1, -γ2) by tyrosine phosphorylation is fundamental to the control of diverse biological processes, including chemotaxis, platelet aggregation, and adaptive immunity. In turn, aberrant activation of PLC-γ1 and PLC-γ2 is implicated in inflammation, autoimmu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51700
更新日期:2019-12-31 00:00:00
abstract::RNA molecules cause the proteins involved in the formation of germ granules to coalesce into liquid droplets. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.24106
更新日期:2017-01-24 00:00:00
abstract::We previously showed in Drosophila melanogaster embryos that low-affinity Ultrabithorax (Ubx)-responsive shavenbaby (svb) enhancers drive expression using localized transcriptional environments and that active svb enhancers on different chromosomes tended to colocalize (Tsai et al., 2017). Here, we test the hypothesis...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45325
更新日期:2019-07-11 00:00:00
abstract::Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remai...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.35621
更新日期:2018-06-25 00:00:00
abstract::Calcium is a critical second messenger in neurons that contributes to learning and memory, but how the coordination of action potentials of neuronal ensembles with the hippocampal local field potential (LFP) is reflected in dynamic calcium activity remains unclear. Here, we recorded hippocampal calcium activity with e...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.39777
更新日期:2019-03-07 00:00:00
abstract::WNK1, a kinase that controls kidney salt homeostasis, also regulates adhesion and migration in CD4+ T cells. Wnk1 is highly expressed in thymocytes, and since migration is important for thymocyte maturation, we investigated a role for WNK1 in mouse thymocyte development. We find that WNK1 is required for the transitio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56934
更新日期:2020-10-14 00:00:00
abstract::Dorsal/ventral (DV) patterning of the sea urchin embryo relies on a ventrally-localized organizer expressing Nodal, a pivotal regulator of the DV gene regulatory network. However, the inceptive mechanisms imposing the symmetry-breaking are incompletely understood. In Paracentrotus lividus, the Hbox12 homeodomain-conta...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04664
更新日期:2014-12-02 00:00:00
abstract::In eukaryotes, most integral membrane proteins are synthesized, integrated into the membrane, and folded properly in the endoplasmic reticulum (ER). We screened the mutants affecting rhabdomeric expression of rhodopsin 1 (Rh1) in the Drosophila photoreceptors and found that dPob/EMC3, EMC1, and EMC8/9, Drosophila homo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.06306
更新日期:2015-02-26 00:00:00
abstract::Human pluripotent stem cell (hPSC)-derived neural organoids display unprecedented emergent properties. Yet in contrast to the singular neuroepithelial tube from which the entire central nervous system (CNS) develops in vivo, current organoid protocols yield tissues with multiple neuroepithelial units, a.k.a. neural ro...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.37549
更新日期:2018-10-29 00:00:00
abstract::Psychological theories of suicide suggest that certain traits may reduce aversion to physical threat and increase the probability of transitioning from suicidal ideation to action. Here, we investigated whether blunted sensitivity to bodily signals is associated with suicidal action by comparing individuals with a his...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51593
更新日期:2020-04-07 00:00:00
abstract::The neural control of sugar consumption is critical for normal metabolism. In contrast to sugar-sensing taste neurons that promote consumption, we identify a taste neuron that limits sucrose consumption in Drosophila. Silencing of the neuron increases sucrose feeding; optogenetic activation decreases it. The feeding i...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.24992
更新日期:2017-03-23 00:00:00
abstract::Stem cells are maintained in a specialized microenvironment called niche but the nature of stem cell niche remains poorly defined in many systems. Here we demonstrate that intestinal epithelium-derived BMP serves as a niche signal for intestinal stem cell (ISC) self-renewal in Drosophila adult midgut. We find that BMP...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01857
更新日期:2014-03-11 00:00:00
abstract::Postmitotic tissues are incapable of replacing damaged cells through proliferation, but need to rely on buffering mechanisms to prevent tissue disintegration. By constitutively activating the Ras/MAPK-pathway via RasV12-overexpression in the postmitotic salivary glands of Drosophila larvae, we overrode the glands adap...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.64919
更新日期:2020-12-30 00:00:00
abstract::The basolateral amygdala (BLA) plays a vital role in associating sensory stimuli with salient valence information. Excitatory principal neurons (PNs) undergo plastic changes to encode this association; however, local BLA inhibitory interneurons (INs) gate PN plasticity via feedforward inhibition (FFI). Despite literat...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50601
更新日期:2020-01-09 00:00:00
abstract::Pervasive expression of circular RNA is a recently discovered feature of eukaryotic gene expression programs, yet its function remains largely unknown. The presumed biogenesis of these RNAs involves a non-canonical 'backsplicing' event. Recent studies in mammalian cell culture posit that backsplicing is facilitated by...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07540
更新日期:2015-06-09 00:00:00
abstract::Autoinducers are small signaling molecules that mediate intercellular communication in microbial populations and trigger coordinated gene expression via 'quorum sensing'. Elucidating the mechanisms that control autoinducer production is, thus, pertinent to understanding collective microbial behavior, such as virulence...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25773
更新日期:2017-07-25 00:00:00
abstract::Aberrant expression of cancer genes and non-canonical RNA species is a hallmark of cancer. However, the mechanisms driving such atypical gene expression programs are incompletely understood. Here, our transcriptional profiling of a cohort of 50 primary clear cell renal cell carcinoma (ccRCC) samples from The Cancer Ge...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.09214
更新日期:2015-11-17 00:00:00
abstract::This study examined records of 2566 consecutive COVID-19 patients at five Massachusetts hospitals and sought to predict level-of-care requirements based on clinical and laboratory data. Several classification methods were applied and compared against standard pneumonia severity scores. The need for hospitalization, IC...
journal_title:eLife
pub_type: 杂志文章,多中心研究
doi:10.7554/eLife.60519
更新日期:2020-10-12 00:00:00
abstract::Signaling by the G-protein-coupled receptors (GPCRs) plays fundamental role in a vast number of essential physiological functions. Precise control of GPCR signaling requires action of regulators of G protein signaling (RGS) proteins that deactivate heterotrimeric G proteins. RGS proteins are elaborately regulated and ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.42150
更新日期:2018-12-12 00:00:00
abstract::Transient receptor potential (TRP) ion channels in peripheral sensory neurons are functionally regulated by hydrolysis of the phosphoinositide PI(4,5)P2 and changes in the level of protein kinase mediated phosphorylation following activation of various G protein coupled receptors. We now show that the activity of TRPM...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26138
更新日期:2017-08-15 00:00:00
abstract::The anterior cingulate cortex (ACC) encodes information supporting mnemonic and cognitive processes. We show here that a rat's position can be decoded with high spatiotemporal resolution from ACC activity. ACC neurons encoded the current state of the animal and task, except for brief excursions that sometimes occurred...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.29793
更新日期:2018-04-17 00:00:00