相关文献
eLife文献大全abstract::Protein aggregation appears to originate from partially unfolded conformations that are sampled through stochastic fluctuations of the native protein. It has been a challenge to characterize these fluctuations, under native like conditions. Here, the conformational dynamics of the full-length (23-231) mouse prion prot...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44766
更新日期:2019-04-26 00:00:00
abstract::Despite employing diverse molecular mechanisms, many different cell signaling systems avoid losing information by transmitting it in a linear manner. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.41894
更新日期:2018-10-25 00:00:00
abstract::Understanding genome to phenotype linkages has been greatly enabled by genomic sequencing. However, most genome analysis is typically confined to the nuclear genome. We conducted a metabolomic QTL analysis on a reciprocal RIL population structured to examine how variation in the organelle genomes affects phenotypic va...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00776
更新日期:2013-10-08 00:00:00
abstract::The nematode Caenorhabditis elegans possesses a simple embryonic nervous system with few enough neurons that the growth of each cell could be followed to provide a systems-level view of development. However, studies of single cell development have largely been conducted in fixed or pre-twitching live embryos, because ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10070
更新日期:2015-12-03 00:00:00
abstract::There has been a longstanding debate on whether original fear memory is inhibited or erased after extinction. One possibility that reconciles this uncertainty is that the inhibition and erasure mechanisms are engaged in different phases (early or late) of extinction. In this study, using single-session extinction trai...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25224
更新日期:2017-07-03 00:00:00
abstract::Mitochondrial dysfunction is associated with activation of the integrated stress response (ISR) but the underlying triggers remain unclear. We systematically combined acute mitochondrial inhibitors with genetic tools for compartment-specific NADH oxidation to trace mechanisms linking different forms of mitochondrial d...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49178
更新日期:2020-05-28 00:00:00
abstract::Although mRNAs are localized in the processes of excitatory neurons, it is still unclear whether interneurons also localize a large population of mRNAs. In addition, the variability in the localized mRNA population within and between cell types is unknown. Here we describe the unbiased transcriptomic characterization ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.63092
更新日期:2021-01-06 00:00:00
abstract::We present the development and in vivo application of a feedback-based tracking microscope to follow individual mitochondria in sensory neurons of zebrafish larvae with nanometer precision and millisecond temporal resolution. By combining various technical improvements, we tracked individual mitochondria with unpreced...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46059
更新日期:2019-06-10 00:00:00
abstract::Bacterial cells utilize monitoring substrates, which undergo force-sensitive translation elongation arrest, to feedback-regulate a Sec-related gene. Vibrio alginolyticus VemP controls the expression of SecD/F that stimulates a late step of translocation by undergoing export-regulated elongation arrest. Here, we attemp...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.62623
更新日期:2020-12-15 00:00:00
abstract::Being exposed to cannabinoids in the womb has different consequences for male and female rats. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.41229
更新日期:2018-09-28 00:00:00
abstract::RNA molecules cause the proteins involved in the formation of germ granules to coalesce into liquid droplets. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.24106
更新日期:2017-01-24 00:00:00
abstract::Perturbation of neuronal activity is key to understanding the brain's functional properties, however, intervention studies typically perturb neurons in a nonspecific manner. Recent optogenetics techniques have enabled patterned perturbations, in which specific patterns of activity can be invoked in identified target n...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52757
更新日期:2020-02-19 00:00:00
abstract::Aberrant expression of cancer genes and non-canonical RNA species is a hallmark of cancer. However, the mechanisms driving such atypical gene expression programs are incompletely understood. Here, our transcriptional profiling of a cohort of 50 primary clear cell renal cell carcinoma (ccRCC) samples from The Cancer Ge...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.09214
更新日期:2015-11-17 00:00:00
abstract::Physiological adaptation to proteotoxic stress in the endoplasmic reticulum (ER) requires retrotranslocation of misfolded proteins into the cytoplasm for ubiquitination and elimination by ER-associated degradation (ERAD). A surprising paradox emerging from recent studies is that ubiquitin ligases (E3s) and deubiquitin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01369
更新日期:2014-01-01 00:00:00
abstract::The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed 'Opto-CRAC') that selectively ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10024
更新日期:2015-12-08 00:00:00
abstract::CRISPR-based homing gene drives have sparked both enthusiasm and deep concerns due to their potential for genetically altering entire species. This raises the question about our ability to prevent the unintended spread of such drives from the laboratory into a natural population. Here, we experimentally demonstrate th...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41439
更新日期:2019-01-22 00:00:00
abstract::Chemosensory neurons extract information about chemical cues from the environment. How is the activity in these sensory neurons transformed into behavior? Using Caenorhabditis elegans, we map a novel sensory neuron circuit motif that encodes odor concentration. Primary neurons, AWC(ON) and AWA, directly detect the foo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10181
更新日期:2015-09-22 00:00:00
abstract::Proteins can self-organize into spatial patterns via non-linear dynamic interactions on cellular membranes. Modelling and simulations have shown that small GTPases can generate patterns by coupling guanine nucleotide exchange factors (GEF) to effectors, generating a positive feedback of GTPase activation and membrane ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54434
更新日期:2020-06-08 00:00:00
abstract::Using a clever combination of genetic and biochemical tools researchers have shown that a transcription factor called USF1 has a central role in determining how mutations of the Clock gene manifest themselves in the behaviour of different mouse strains. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.00659
更新日期:2013-04-09 00:00:00
abstract::During inflammation, serum amyloid A proteins transport retinol to infected tissues. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.04246
更新日期:2014-09-02 00:00:00
abstract::Innate lymphoid cells (ILCs) contribute to host defence and tissue repair but can induce immunopathology. Recent work has revealed tissue-specific roles for ILCs; however, the question of how a small population has large effects on immune homeostasis remains unclear. We identify two mechanisms that ILC3s utilise to ex...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10066
更新日期:2016-01-18 00:00:00
abstract::Altered insulin signaling has been linked to widespread nervous system dysfunction including cognitive dysfunction, neuropathy and susceptibility to neurodegenerative disease. However, knowledge of the cellular mechanisms underlying the effects of insulin on neuronal function is incomplete. Here, we show that cell aut...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16807
更新日期:2016-08-15 00:00:00
abstract::Hybrid sterility is one of the earliest postzygotic isolating mechanisms to evolve between two recently diverged species. Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha, which mate to form viable hybrid diploids that effici...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.02630
更新日期:2014-06-24 00:00:00
abstract::Variation in the presence or absence of transposable elements (TEs) is a major source of genetic variation between individuals. Here, we identified 23,095 TE presence/absence variants between 216 Arabidopsis accessions. Most TE variants were rare, and we find these rare variants associated with local extremes of gene ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20777
更新日期:2016-12-02 00:00:00
abstract::Cortical plasticity is fundamental to motor recovery following cortical perturbation. However, it is still unclear how this plasticity is induced at a functional circuit level. Here, we investigated motor recovery and underlying neural plasticity upon optogenetic suppression of a cortical area for eye movement. Using ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50855
更新日期:2019-11-05 00:00:00
abstract::While specific mutations allow organisms to adapt to stressful environments, most changes in an organism's DNA negatively impact fitness. The mutation rate is therefore strictly regulated and often considered a slowly-evolving parameter. In contrast, we demonstrate an unexpected flexibility in cellular mutation rates ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22939
更新日期:2017-05-02 00:00:00
abstract::The β-catenin transcriptional coregulator is involved in various biological and pathological processes; however, its requirements in hematopoietic cells remain controversial. We re-targeted the Ctnnb1 gene locus to generate a true β-catenin-null mutant mouse strain. Ablation of β-catenin alone, or in combination with ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.55360
更新日期:2020-08-21 00:00:00
abstract::Recent work draws attention to community-community encounters ('coalescence') as likely an important factor shaping natural ecosystems. This work builds on MacArthur's classic model of competitive coexistence to investigate such community-level competition in a minimal theoretical setting. It is shown that the ability...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15747
更新日期:2016-06-16 00:00:00
abstract::Goal-directed behavior involves distributed neuronal circuits in the mammalian brain, including diverse regions of neocortex. However, the cellular basis of long-range cortico-cortical signaling during goal-directed behavior is poorly understood. Here, we recorded membrane potential of excitatory layer 2/3 pyramidal n...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15798
更新日期:2016-06-21 00:00:00
abstract::Adult-born neurons are continually produced in the dentate gyrus but it is unclear whether synaptic integration of new neurons affects the pre-existing circuit. Here we investigated how manipulating neurogenesis in adult mice alters excitatory synaptic transmission to mature dentate neurons. Enhancing neurogenesis by ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19886
更新日期:2017-01-30 00:00:00