Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons.

abstract：:Cranial neural crest (CNC) cells give rise to bone, cartilage, tendons, and ligaments of the vertebrate craniofacial musculoskeletal complex, as well as regulate mesoderm-derived craniofacial muscle development through cell-cell interactions. Using the mouse soft palate as a model, we performed an unbiased single-cell...

journal_title：eLife

authors： Han X,Feng J,Guo T,Loh YE,Yuan Y,Ho TV,Cho CK,Li J,Jing J,Janeckova E,He J,Pei F,Bi J,Song B,Chai Y

更新日期：2021-01-22 00:00:00

abstract：:In plants the dorsoventral boundary of leaves defines an axis of symmetry through the centre of the organ separating the top (dorsal) and bottom (ventral) tissues. Although the positioning of this boundary is critical for leaf morphogenesis, how the boundary is established and how it influences development remains unc...

journal_title：eLife

authors： Caggiano MP,Yu X,Bhatia N,Larsson A,Ram H,Ohno CK,Sappl P,Meyerowitz EM,Jönsson H,Heisler MG

更新日期：2017-09-12 00:00:00

abstract：:We present the development and in vivo application of a feedback-based tracking microscope to follow individual mitochondria in sensory neurons of zebrafish larvae with nanometer precision and millisecond temporal resolution. By combining various technical improvements, we tracked individual mitochondria with unpreced...

journal_title：eLife

authors： Wehnekamp F,Plucińska G,Thong R,Misgeld T,Lamb DC

更新日期：2019-06-10 00:00:00

abstract：:Some cortical neurons receive highly selective thalamocortical (TC) input, but others do not. Here, we examine connectivity of single thalamic neurons (lateral geniculate nucleus, LGN) onto putative fast-spike inhibitory interneurons in layer 4 of rabbit visual cortex. We show that three 'rules' regulate this connecti...

journal_title：eLife

authors： Bereshpolova Y,Hei X,Alonso JM,Swadlow HA

更新日期：2020-12-08 00:00:00

abstract：:Heterochromatin formed by the SUV39 histone methyltransferases represses transcription from repetitive DNA sequences and ensures genomic stability. How SUV39 enzymes localize to their target genomic loci remains unclear. Here, we demonstrate that chromatin-associated RNA contributes to the stable association of SUV39H...

journal_title：eLife

authors： Johnson WL,Yewdell WT,Bell JC,McNulty SM,Duda Z,O'Neill RJ,Sullivan BA,Straight AF

更新日期：2017-08-01 00:00:00

abstract：:In endoplasmic reticulum-associated protein degradation (ERAD), membrane proteins are ubiquitinated, extracted from the membrane, and degraded by the proteasome. The cytosolic ATPase Cdc48 drives extraction by pulling on polyubiquitinated substrates. How hydrophobic transmembrane (TM) segments are moved from the phosp...

journal_title：eLife

authors： Schmidt CC,Vasic V,Stein A

更新日期：2020-06-26 00:00:00

abstract：:C-type inactivation of potassium channels fine-tunes the electrical signaling in excitable cells through an internal timing mechanism that is mediated by a hydrogen bond network in the channels' selectively filter. Previously, we used nonsense suppression to highlight the role of the conserved Trp434-Asp447 indole hyd...

journal_title：eLife

authors： Lueck JD,Mackey AL,Infield DT,Galpin JD,Li J,Roux B,Ahern CA

更新日期：2016-10-06 00:00:00

abstract：:A hallmark of CRISPR-Cas immunity systems is the CRISPR array, a genomic locus consisting of short, repeated sequences ('repeats') interspersed with short, variable sequences ('spacers'). CRISPR arrays are transcribed and processed into individual CRISPR RNAs that each include a single spacer, and direct Cas proteins ...

journal_title：eLife

authors： Stringer AM,Baniulyte G,Lasek-Nesselquist E,Seed KD,Wade JT

更新日期：2020-10-30 00:00:00

abstract：:Many marine animals, ranging from corals to fishes, synchronise reproduction to lunar cycles. In the annelid Platynereis dumerilii, this timing is orchestrated by an endogenous monthly (circalunar) clock entrained by moonlight. Whereas daily (circadian) clocks cause extensive transcriptomic and proteomic changes, the ...

journal_title：eLife

authors： Schenk S,Bannister SC,Sedlazeck FJ,Anrather D,Minh BQ,Bileck A,Hartl M,von Haeseler A,Gerner C,Raible F,Tessmar-Raible K

更新日期：2019-02-15 00:00:00

abstract：:The neuronal DNA-/RNA-binding protein Pur-alpha is a transcription regulator and core factor for mRNA localization. Pur-alpha-deficient mice die after birth with pleiotropic neuronal defects. Here, we report the crystal structure of the DNA-/RNA-binding domain of Pur-alpha in complex with ssDNA. It reveals base-specif...

journal_title：eLife

authors： Weber J,Bao H,Hartlmüller C,Wang Z,Windhager A,Janowski R,Madl T,Jin P,Niessing D

更新日期：2016-01-08 00:00:00

abstract：:K146N is a dominant mutation in human β-cardiac myosin heavy chain, which causes hypertrophic cardiomyopathy. We examined how Drosophila muscle responds to this mutation and integratively analyzed the biochemical, physiological and mechanical foundations of the disease. ATPase assays, actin motility, and indirect flig...

journal_title：eLife

authors： Kronert WA,Bell KM,Viswanathan MC,Melkani GC,Trujillo AS,Huang A,Melkani A,Cammarato A,Swank DM,Bernstein SI

更新日期：2018-08-13 00:00:00

abstract：:In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood. To understand the time-dependent scaling dynamics we treated cultured rat hippocampal cells wi...

journal_title：eLife

authors： Schanzenbächer CT,Langer JD,Schuman EM

更新日期：2018-02-15 00:00:00

abstract：:To effectively interact with their environment, humans must often select actions from multiple incompatible options. Existing theories propose that during motoric response-conflict, inappropriate motor activity is actively (and perhaps non-selectively) suppressed by an inhibitory fronto-basal ganglia mechanism. We her...

journal_title：eLife

authors： Wessel JR,Waller DA,Greenlee JD

更新日期：2019-05-07 00:00:00

abstract：:TMEM16F is activated by elevated intracellular Ca2+, and functions as a small-conductance ion channel and as a phospholipid scramblase. In contrast to its paralogs, the TMEM16A/B calcium-activated chloride channels, mouse TMEM16F has been reported as a cation-, anion-, or non-selective ion channel, without a definite ...

journal_title：eLife

authors： Ye W,Han TW,He M,Jan YN,Jan LY

更新日期：2019-07-18 00:00:00

abstract：:Heterogeneity broadly exists in various cell types both during development and at homeostasis. Investigating heterogeneity is crucial for comprehensively understanding the complexity of ontogeny, dynamics, and function of specific cell types. Traditional bulk-labeling techniques are incompetent to dissect heterogeneit...

journal_title：eLife

authors： He S,Tian Y,Feng S,Wu Y,Shen X,Chen K,He Y,Sun Q,Li X,Xu J,Wen Z,Qu JY

更新日期：2020-01-06 00:00:00

abstract：:A major puzzle in biology is how mammalian sperm maintain the correct swimming direction during various phases of the sexual reproduction process. Whilst chemotaxis may dominate near the ovum, it is unclear which cues guide spermatozoa on their long journey towards the egg. Hypothesized mechanisms range from peristalt...

journal_title：eLife

authors： Kantsler V,Dunkel J,Blayney M,Goldstein RE

更新日期：2014-05-27 00:00:00

abstract：:Coat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, linking them to the outer COPII components that form a cage around the vesicle. Regulated flexibility in coat architect...

journal_title：eLife

authors： Zanetti G,Prinz S,Daum S,Meister A,Schekman R,Bacia K,Briggs JA

更新日期：2013-09-17 00:00:00

abstract：:In most vertebrate neurons, action potentials are triggered at the distal end of the axon initial segment (AIS). Both position and length of the AIS vary across and within neuron types, with activity, development and pathology. What is the impact of AIS geometry on excitability? Direct empirical assessment has proven ...

journal_title：eLife

authors： Goethals S,Brette R

更新日期：2020-03-30 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) have been shown to act as important cell biological regulators including cell fate decisions but are often ignored in human genetics. Combining differential lncRNA expression during neuronal lineage induction with copy number variation morbidity maps of a cohort of children with autism sp...

journal_title：eLife

authors： Ang CE,Ma Q,Wapinski OL,Fan S,Flynn RA,Lee QY,Coe B,Onoguchi M,Olmos VH,Do BT,Dukes-Rimsky L,Xu J,Tanabe K,Wang L,Elling U,Penninger JM,Zhao Y,Qu K,Eichler EE,Srivastava A,Wernig M,Chang HY

更新日期：2019-01-10 00:00:00

abstract：:India comprises much of the persisting global childhood measles mortality. India implemented a mass second-dose measles immunization campaign in 2010. We used interrupted time series and multilevel regression to quantify the campaign's impact on measles mortality using the nationally representative Million Death Study...

journal_title：eLife

authors： Wong BK,Fadel SA,Awasthi S,Khera A,Kumar R,Menon G,Jha P

更新日期：2019-03-05 00:00:00

abstract：:Whether neurons encode information through their spike rates, their activity times or both is an ongoing debate in systems neuroscience. Here, we tested whether humans can discriminate between a pair of temporal odor mixtures (TOMs) composed of the same two components delivered in rapid succession in either one tempor...

journal_title：eLife

authors： Perl O,Nahum N,Belelovsky K,Haddad R

更新日期：2020-02-07 00:00:00

abstract：:Phenazines are natural bacterial antibiotics that can protect crops from disease. However, for most crops it is unknown which producers and specific phenazines are ecologically relevant, and whether phenazine biodegradation can counter their effects. To better understand their ecology, we developed and environmentally...

journal_title：eLife

authors： Dar D,Thomashow LS,Weller DM,Newman DK

更新日期：2020-09-15 00:00:00

abstract：:Most neurogenesis in the mammalian brain is completed embryonically, but in certain areas the production of neurons continues throughout postnatal life. The functional properties of mature postnatally generated neurons often match those of their embryonically produced counterparts. However, we show here that in the ol...

journal_title：eLife

authors： Galliano E,Franzoni E,Breton M,Chand AN,Byrne DJ,Murthy VN,Grubb MS

更新日期：2018-04-20 00:00:00

abstract：:Arm-amputation involves two powerful drivers for brain plasticity-sensory deprivation and altered use. However, research has largely focused on sensory deprivation and maladaptive change. Here we show that adaptive patterns of limb usage after amputation drive cortical plasticity. We report that individuals with conge...

journal_title：eLife

authors： Makin TR,Cramer AO,Scholz J,Hahamy A,Henderson Slater D,Tracey I,Johansen-Berg H

更新日期：2013-11-12 00:00:00

abstract：:Anterograde transport of late endosomes or lysosomes (LE/Lys) is crucial for proper axon growth. However, the role of energetic nutrients has been poorly explored. Malonyl-CoA is a precursor of fatty acids, and its intracellular levels highly fluctuate depending on glucose availability or the energy sensor AMP-activat...

journal_title：eLife

authors： Palomo-Guerrero M,Fadó R,Casas M,Pérez-Montero M,Baena M,Helmer PO,Domínguez JL,Roig A,Serra D,Hayen H,Stenmark H,Raiborg C,Casals N

更新日期：2019-12-23 00:00:00

abstract：:The rhythm generating network for breathing must continuously adjust to changing metabolic and behavioral demands. Here, we examined network-based mechanisms in the mouse preBötzinger complex using substance P, a potent excitatory modulator of breathing frequency and stability, as a tool to dissect network properties ...

journal_title：eLife

authors： Baertsch NA,Ramirez JM

更新日期：2019-12-05 00:00:00

abstract：:Human ascariasis is a major neglected tropical disease caused by the nematode Ascaris lumbricoides. We report a 296 megabase (Mb) reference-quality genome comprised of 17,902 protein-coding genes derived from a single, representative Ascaris worm. An additional 68 worms were collected from 60 human hosts in Kenyan vil...

journal_title：eLife

authors： Easton A,Gao S,Lawton SP,Bennuru S,Khan A,Dahlstrom E,Oliveira RG,Kepha S,Porcella SF,Webster J,Anderson R,Grigg ME,Davis RE,Wang J,Nutman TB

更新日期：2020-11-06 00:00:00

abstract：:Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional equivalents across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role...

journal_title：eLife

authors： Rogala KB,Dynes NJ,Hatzopoulos GN,Yan J,Pong SK,Robinson CV,Deane CM,Gönczy P,Vakonakis I

更新日期：2015-05-29 00:00:00

abstract：:INAD assembles key enzymes of the Drosophila compound eye photo-transduction pathway into a supramolecular complex, supporting efficient and fast light signaling. However, the molecular mechanism that governs the interaction between INAD and NORPA (phospholipase Cβ, PLCβ), a key step for the fast kinetics of the light...

journal_title：eLife

authors： Ye F,Huang Y,Li J,Ma Y,Xie C,Liu Z,Deng X,Wan J,Xue T,Liu W,Zhang M

更新日期：2018-12-10 00:00:00

abstract：:Generating recombinant monoclonal antibodies (R-mAbs) from mAb-producing hybridomas offers numerous advantages that increase the effectiveness, reproducibility, and transparent reporting of research. We report here the generation of a novel resource in the form of a library of recombinant R-mAbs validated for neurosci...

journal_title：eLife

authors： Andrews NP,Boeckman JX,Manning CF,Nguyen JT,Bechtold H,Dumitras C,Gong B,Nguyen K,van der List D,Murray KD,Engebrecht J,Trimmer JS

更新日期：2019-01-22 00:00:00