Atomic mutagenesis in ion channels with engineered stoichiometry.

abstract：:More than half of long-term brain tumor survivors develop irreversible cognitive decline that severely affect their quality of life. However, there is no pre-clinical model that allows long-term assessment of cognition, and there is no treatment which ameliorates cognitive deficits in patients. Here, we report a novel...

journal_title：eLife

authors： Feng X,Liu S,Chen D,Rosi S,Gupta N

更新日期：2018-11-13 00:00:00

abstract：:Cardiac conduction defects decrease life expectancy in myotonic dystrophy type 1 (DM1), a CTG repeat disorder involving misbalance between two RNA binding factors, MBNL1 and CELF1. However, how DM1 condition translates into conduction disorders remains poorly understood. Here we simulated MBNL1 and CELF1 misbalance in...

journal_title：eLife

authors： Auxerre-Plantié E,Nakamori M,Renaud Y,Huguet A,Choquet C,Dondi C,Miquerol L,Takahashi MP,Gourdon G,Junion G,Jagla T,Zmojdzian M,Jagla K

更新日期：2019-12-12 00:00:00

abstract：:Predicting and constraining RNA virus evolution require understanding the molecular factors that define the mutational landscape accessible to these pathogens. RNA viruses typically have high mutation rates, resulting in frequent production of protein variants with compromised biophysical properties. Their evolution i...

journal_title：eLife

authors： Phillips AM,Gonzalez LO,Nekongo EE,Ponomarenko AI,McHugh SM,Butty VL,Levine SS,Lin YS,Mirny LA,Shoulders MD

更新日期：2017-09-26 00:00:00

abstract：:In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches ...

journal_title：eLife

authors： Yin Y,Dominska M,Yim E,Petes TD

更新日期：2017-07-17 00:00:00

abstract：:Two gene variants provide different levels of protection against sleeping sickness, but this comes with an increased risk of developing chronic kidney disease. ...

journal_title：eLife

authors： Kruzel-Davila E,Skorecki K

更新日期：2017-07-03 00:00:00

abstract：:Circadian rhythms are biological oscillations that schedule daily changes in physiology. Outside the laboratory, circadian clocks do not generally free-run but are driven by daily cues whose timing varies with the seasons. The principles that determine how circadian clocks align to these external cycles are not well u...

journal_title：eLife

authors： Leypunskiy E,Lin J,Yoo H,Lee U,Dinner AR,Rust MJ

更新日期：2017-07-07 00:00:00

abstract：: ...

journal_title：eLife

authors： DeAngelis BD,Zavatone-Veth JA,Clark DA

更新日期：2020-12-04 00:00:00

abstract：:Macrophages derive from multiple sources of hematopoietic progenitors. Most macrophages require colony-stimulating factor 1 receptor (CSF1R), but some macrophages persist in the absence of CSF1R. Here, we analyzed mpeg1:GFP-expressing macrophages in csf1r-deficient zebrafish and report that embryonic macrophages emerg...

journal_title：eLife

authors： Kuil LE,Oosterhof N,Ferrero G,Mikulášová T,Hason M,Dekker J,Rovira M,van der Linde HC,van Strien PM,de Pater E,Schaaf G,Bindels EM,Wittamer V,van Ham TJ

更新日期：2020-05-05 00:00:00

abstract：:Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature of this pro-inflammatory response in mammals is the complex remodeling of cellular metabolism towards aerobic glycolysis. Although the function of bactericidal macrophages is highly con...

journal_title：eLife

authors： Krejčová G,Danielová A,Nedbalová P,Kazek M,Strych L,Chawla G,Tennessen JM,Lieskovská J,Jindra M,Doležal T,Bajgar A

更新日期：2019-10-14 00:00:00

abstract：:Brief periods of sleep loss have long-lasting consequences such as impaired memory consolidation. Structural changes in synaptic connectivity have been proposed as a substrate of memory storage. Here, we examine the impact of brief periods of sleep deprivation on dendritic structure. In mice, we find that five hours o...

journal_title：eLife

authors： Havekes R,Park AJ,Tudor JC,Luczak VG,Hansen RT,Ferri SL,Bruinenberg VM,Poplawski SG,Day JP,Aton SJ,Radwańska K,Meerlo P,Houslay MD,Baillie GS,Abel T

更新日期：2016-08-23 00:00:00

abstract：:Cell-cell communication in multicellular organisms depends on the dynamic and reversible phosphorylation of protein tyrosine residues. The receptor-linked protein tyrosine phosphatases (RPTPs) receive cues from the extracellular environment and are well placed to influence cell signaling. However, the direct events do...

journal_title：eLife

authors： Fearnley GW,Young KA,Edgar JR,Antrobus R,Hay IM,Liang WC,Martinez-Martin N,Lin W,Deane JE,Sharpe HJ

更新日期：2019-03-29 00:00:00

abstract：:WW domain tandem-containing proteins such as KIBRA, YAP, and MAGI play critical roles in cell growth and polarity via binding to and positioning target proteins in specific subcellular regions. An immense disparity exists between promiscuity of WW domain-mediated target bindings and specific roles of WW domain protein...

journal_title：eLife

authors： Lin Z,Yang Z,Xie R,Ji Z,Guan K,Zhang M

更新日期：2019-09-05 00:00:00

abstract：:Evolutionary changes in transcription networks are an important source of diversity across species, yet the quantitative consequences of network evolution have rarely been studied. Here we consider the transcriptional 'rewiring' of the three GAL genes that encode the enzymes needed for cells to convert galactose to gl...

journal_title：eLife

authors： Dalal CK,Zuleta IA,Mitchell KF,Andes DR,El-Samad H,Johnson AD

更新日期：2016-09-10 00:00:00

abstract：:The roles of long non-coding RNAs (lncRNAs) in regulating cancer and stem cells are being increasingly appreciated. Its diverse mechanisms provide the regulatory network with a bigger repertoire to increase complexity. Here we report a novel LncRNA, Lnc34a, that is enriched in colon cancer stem cells (CCSCs) and initi...

journal_title：eLife

authors： Wang L,Bu P,Ai Y,Srinivasan T,Chen HJ,Xiang K,Lipkin SM,Shen X

更新日期：2016-04-14 00:00:00

abstract：:The Sae2/CtIP protein is required for efficient processing of DNA double-strand breaks that initiate homologous recombination in eukaryotic cells. Sae2/CtIP is also important for survival of single-stranded Top1-induced lesions and CtIP is known to associate directly with transcription-associated complexes in mammalia...

journal_title：eLife

authors： Makharashvili N,Arora S,Yin Y,Fu Q,Wen X,Lee JH,Kao CH,Leung JW,Miller KM,Paull TT

更新日期：2018-12-07 00:00:00

abstract：:Antiviral effectors such as natural killer (NK) cells have impaired functions in chronic hepatitis B (CHB) patients. The molecular mechanism responsible for this dysfunction remains poorly characterised. We show that decreased cytokine production capacity of peripheral NK cells from CHB patients was associated with re...

journal_title：eLife

authors： Marotel M,Villard M,Drouillard A,Tout I,Besson L,Allatif O,Pujol M,Rocca Y,Ainouze M,Roblot G,Viel S,Gomez M,Loustaud V,Alain S,Durantel D,Walzer T,Hasan U,Marçais A

更新日期：2021-01-28 00:00:00

abstract：:Synaptic adhesion molecules regulate synapse development and function. However, whether and how presynaptic adhesion molecules regulate postsynaptic NMDAR function remains largely unclear. Presynaptic LAR family receptor tyrosine phosphatases (LAR-RPTPs) regulate synapse development through mechanisms that include tra...

journal_title：eLife

authors： Kim K,Shin W,Kang M,Lee S,Kim D,Kang R,Jung Y,Cho Y,Yang E,Kim H,Bae YC,Kim E

更新日期：2020-03-06 00:00:00

abstract：:The COVID-19 pandemic has resulted in school closures and distancing requirements that have disrupted both work and family life for many. Concerns exist that these disruptions caused by the pandemic may not have influenced men and women researchers equally. Many medical journals have published papers on the pandemic, ...

journal_title：eLife

authors： Andersen JP,Nielsen MW,Simone NL,Lewiss RE,Jagsi R

更新日期：2020-06-15 00:00:00

abstract：:Being exposed to cannabinoids in the womb has different consequences for male and female rats. ...

journal_title：eLife

authors： Musardo S,Bellone C

更新日期：2018-09-28 00:00:00

abstract：:The neuronal DNA-/RNA-binding protein Pur-alpha is a transcription regulator and core factor for mRNA localization. Pur-alpha-deficient mice die after birth with pleiotropic neuronal defects. Here, we report the crystal structure of the DNA-/RNA-binding domain of Pur-alpha in complex with ssDNA. It reveals base-specif...

journal_title：eLife

authors： Weber J,Bao H,Hartlmüller C,Wang Z,Windhager A,Janowski R,Madl T,Jin P,Niessing D

更新日期：2016-01-08 00:00:00

abstract：:Although seasonality is widespread and can cause fluctuations in the intensity and direction of natural selection, we have little information about the consequences of seasonal fitness trade-offs for population dynamics. Here we exposed populations of Drosophila melanogaster to repeated seasonal changes in resources a...

journal_title：eLife

authors： Betini GS,McAdam AG,Griswold CK,Norris DR

更新日期：2017-02-06 00:00:00

abstract：:Axons span extreme distances and are subject to significant stretch deformations during limb movements or sudden head movements, especially during impacts. Yet, axon biomechanics, and its relation to the ultrastructure that allows axons to withstand mechanical stress, is poorly understood. Using a custom developed for...

journal_title：eLife

authors： Dubey S,Bhembre N,Bodas S,Veer S,Ghose A,Callan-Jones A,Pullarkat P

更新日期：2020-04-08 00:00:00

abstract：:Islet amyloidosis by IAPP contributes to pancreatic β-cell death in diabetes, but the nature of toxic IAPP species remains elusive. Using concurrent time-resolved biophysical and biological measurements, we define the toxic species produced during IAPP amyloid formation and link their properties to induction of rat IN...

journal_title：eLife

authors： Abedini A,Plesner A,Cao P,Ridgway Z,Zhang J,Tu LH,Middleton CT,Chao B,Sartori DJ,Meng F,Wang H,Wong AG,Zanni MT,Verchere CB,Raleigh DP,Schmidt AM

更新日期：2016-05-23 00:00:00

abstract：:The investigation of prosocial behavior is of particular interest from an evolutionary perspective. Comparisons of prosociality across non-human animal species have, however, so far largely focused on primates, and their interpretation is hampered by the diversity of paradigms and procedures used. Here, we present the...

journal_title：eLife

authors： Horn L,Bugnyar T,Griesser M,Hengl M,Izawa EI,Oortwijn T,Rössler C,Scheer C,Schiestl M,Suyama M,Taylor AH,Vanhooland LC,von Bayern AM,Zürcher Y,Massen JJ

更新日期：2020-10-20 00:00:00

abstract：:A protein called P36 holds the key to how different species of malaria parasite invade liver cells. ...

journal_title：eLife

authors： Billker O

更新日期：2017-06-14 00:00:00

abstract：:Nutritional regulation by ants emerges from a distributed process: food is collected by a small fraction of workers, stored within the crops of individuals, and spread via local ant-to-ant interactions. The precise individual-level underpinnings of this collective regulation have remained unclear mainly due to difficu...

journal_title：eLife

authors： Greenwald EE,Baltiansky L,Feinerman O

更新日期：2018-03-06 00:00:00

abstract：:Using FIB-SEM we report the entire synaptic connectome of glomerulus VA1v of the right antennal lobe in Drosophila melanogaster. Within the glomerulus we densely reconstructed all neurons, including hitherto elusive local interneurons. The fruitless-positive, sexually dimorphic VA1v included >11,140 presynaptic sites ...

journal_title：eLife

authors： Horne JA,Langille C,McLin S,Wiederman M,Lu Z,Xu CS,Plaza SM,Scheffer LK,Hess HF,Meinertzhagen IA

更新日期：2018-11-01 00:00:00

abstract：:We have previously demonstrated that checkpoint kinase 2 (CHK2) is a critical negative regulator of androgen receptor (AR) transcriptional activity, prostate cancer (PCa) cell growth, and androgen sensitivity. We have now uncovered that the AR directly interacts with CHK2 and ionizing radiation (IR) increases this int...

journal_title：eLife

authors： Ta HQ,Dworak N,Ivey ML,Roller DG,Gioeli D

更新日期：2020-06-24 00:00:00

abstract：:Behavior relies on the ability of sensory systems to infer properties of the environment from incoming stimuli. The accuracy of inference depends on the fidelity with which behaviorally relevant properties of stimuli are encoded in neural responses. High-fidelity encodings can be metabolically costly, but low-fidelity...

journal_title：eLife

authors： Młynarski WF,Hermundstad AM

更新日期：2018-07-10 00:00:00

abstract：:Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactio...

journal_title：eLife

authors： Bawa S,Brooks DS,Neville KE,Tipping M,Sagar MA,Kollhoff JA,Chawla G,Geisbrecht BV,Tennessen JM,Eliceiri KW,Geisbrecht ER

更新日期：2020-03-30 00:00:00