Abstract:
:Evolutionary changes in transcription networks are an important source of diversity across species, yet the quantitative consequences of network evolution have rarely been studied. Here we consider the transcriptional 'rewiring' of the three GAL genes that encode the enzymes needed for cells to convert galactose to glucose. In Saccharomyces cerevisiae, the transcriptional regulator Gal4 binds and activates these genes. In the human pathogen Candida albicans (which last shared a common ancestor with S. cerevisiae some 300 million years ago), we show that different regulators, Rtg1 and Rtg3, activate the three GAL genes. Using single-cell dynamics and RNA-sequencing, we demonstrate that although the overall logic of regulation is the same in both species-the GAL genes are induced by galactose-there are major differences in both the quantitative response of these genes to galactose and in the position of these genes in the overall transcription network structure of the two species.
journal_name
Elifejournal_title
eLifeauthors
Dalal CK,Zuleta IA,Mitchell KF,Andes DR,El-Samad H,Johnson ADdoi
10.7554/eLife.18981subject
Has Abstractpub_date
2016-09-10 00:00:00issn
2050-084Xjournal_volume
5pub_type
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