The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion.

abstract：:Previously, we showed that serum and monocytes from patients with CF exhibit an enhanced NLRP3-inflammasome signature with increased IL-18, IL-1β, caspase-1 activity and ASC speck release (Scambler et al. eLife 2019). Here we show that CFTR modulators down regulate this exaggerated proinflammatory response following L...

journal_title：eLife

authors： Jarosz-Griffiths HH,Scambler T,Wong CH,Lara-Reyna S,Holbrook J,Martinon F,Savic S,Whitaker P,Etherington C,Spoletini G,Clifton I,Mehta A,McDermott MF,Peckham D

更新日期：2020-03-02 00:00:00

abstract：:In the developing fruit fly brain, a protein called Trithorax increases the number of neural cells produced from a single stem cell, in part by regulating the transcription of the target genes buttonhead and pointed. ...

journal_title：eLife

authors： Jiang Y,Reichert H

更新日期：2014-11-06 00:00:00

abstract：:Wnt plays important role during development and in various diseases. Because Wnts are lipidated and highly hydrophobic, they can only be purified in the presence of detergents, limiting their use in various in vitro and in vivo assays. We purified N-terminally tagged recombinant Wnt3a secreted from cells and accidenta...

journal_title：eLife

authors： Mihara E,Hirai H,Yamamoto H,Tamura-Kawakami K,Matano M,Kikuchi A,Sato T,Takagi J

更新日期：2016-02-23 00:00:00

abstract：:The Sae2/CtIP protein is required for efficient processing of DNA double-strand breaks that initiate homologous recombination in eukaryotic cells. Sae2/CtIP is also important for survival of single-stranded Top1-induced lesions and CtIP is known to associate directly with transcription-associated complexes in mammalia...

journal_title：eLife

authors： Makharashvili N,Arora S,Yin Y,Fu Q,Wen X,Lee JH,Kao CH,Leung JW,Miller KM,Paull TT

更新日期：2018-12-07 00:00:00

abstract：:IRE1α is an endoplasmic reticulum (ER) localized endonuclease activated by misfolded proteins in the ER. Previously, we demonstrated that IRE1α forms a complex with the Sec61 translocon, to which its substrate XBP1u mRNA is recruited for cleavage during ER stress (Plumb et al., 2015). Here, we probe IRE1α complexes in...

journal_title：eLife

authors： Sundaram A,Plumb R,Appathurai S,Mariappan M

更新日期：2017-05-15 00:00:00

abstract：:Peptidoglycan (PG) is the main component of bacterial cell walls and the target for many antibiotics. PG biosynthesis is tightly coordinated with cell wall growth and turnover, and many of these control activities depend upon PASTA-domain containing eukaryotic-like serine/threonine protein kinases (PASTA-eSTK) that se...

journal_title：eLife

authors： Wamp S,Rutter ZJ,Rismondo J,Jennings CE,Möller L,Lewis RJ,Halbedel S

更新日期：2020-05-29 00:00:00

abstract：:Seasonal influenza virus A/H3N2 is a major cause of death globally. Vaccination remains the most effective preventative. Rapid mutation of hemagglutinin allows viruses to escape adaptive immunity. This antigenic drift necessitates regular vaccine updates. Effective vaccine strains need to represent H3N2 populations ci...

journal_title：eLife

authors： Huddleston J,Barnes JR,Rowe T,Xu X,Kondor R,Wentworth DE,Whittaker L,Ermetal B,Daniels RS,McCauley JW,Fujisaki S,Nakamura K,Kishida N,Watanabe S,Hasegawa H,Barr I,Subbarao K,Barrat-Charlaix P,Neher RA,Bedford T

更新日期：2020-09-02 00:00:00

abstract：:Khoomei is a unique singing style originating from the republic of Tuva in central Asia. Singers produce two pitches simultaneously: a booming low-frequency rumble alongside a hovering high-pitched whistle-like tone. The biomechanics of this biphonation are not well-understood. Here, we use sound analysis, dynamic mag...

journal_title：eLife

authors： Bergevin C,Narayan C,Williams J,Mhatre N,Steeves JK,Bernstein JG,Story B

更新日期：2020-02-17 00:00:00

abstract：:Plasmodium falciparum causes malaria in humans with over 450,000 deaths annually. The asexual blood stage involves invasion of erythrocytes by merozoites, in which they grow and divide to release daughter merozoites, which in turn invade new erythrocytes perpetuating the cycle responsible for malaria. A key step in me...

journal_title：eLife

authors： Chen L,Xu Y,Wong W,Thompson JK,Healer J,Goddard-Borger ED,Lawrence MC,Cowman AF

更新日期：2017-02-14 00:00:00

abstract：:β-Propellers arise through the amplification of a supersecondary structure element called a blade. This process produces toroids of between four and twelve repeats, which are almost always arranged sequentially in a single polypeptide chain. We found that new propellers evolve continuously by amplification from single...

journal_title：eLife

authors： Afanasieva E,Chaudhuri I,Martin J,Hertle E,Ursinus A,Alva V,Hartmann MD,Lupas AN

更新日期：2019-10-15 00:00:00

abstract：:The precise pattern of motor neuron (MN) activation is essential for the execution of motor actions; however, the molecular mechanisms that give rise to specific patterns of MN activity are largely unknown. Phrenic MNs integrate multiple inputs to mediate inspiratory activity during breathing and are constrained to fi...

journal_title：eLife

authors： Vagnozzi AN,Garg K,Dewitz C,Moore MT,Cregg JM,Jeannotte L,Zampieri N,Landmesser LT,Philippidou P

更新日期：2020-01-16 00:00:00

abstract：:The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...

journal_title：eLife

authors： Lischinsky JE,Sokolowski K,Li P,Esumi S,Kamal Y,Goodrich M,Oboti L,Hammond TR,Krishnamoorthy M,Feldman D,Huntsman M,Liu J,Corbin JG

更新日期：2017-03-13 00:00:00

abstract：:The straight-tusked elephants Palaeoloxodon spp. were widespread across Eurasia during the Pleistocene. Phylogenetic reconstructions using morphological traits have grouped them with Asian elephants (Elephas maximus), and many paleontologists place Palaeoloxodon within Elephas. Here, we report the recovery of full mit...

journal_title：eLife

authors： Meyer M,Palkopoulou E,Baleka S,Stiller M,Penkman KEH,Alt KW,Ishida Y,Mania D,Mallick S,Meijer T,Meller H,Nagel S,Nickel B,Ostritz S,Rohland N,Schauer K,Schüler T,Roca AL,Reich D,Shapiro B,Hofreiter M

更新日期：2017-06-06 00:00:00

abstract：:Striatal dysfunction plays an important role in dystonia, but the striatal cell types that contribute to abnormal movements are poorly defined. We demonstrate that conditional deletion of the DYT1 dystonia protein torsinA in embryonic progenitors of forebrain cholinergic and GABAergic neurons causes dystonic-like twis...

journal_title：eLife

authors： Pappas SS,Darr K,Holley SM,Cepeda C,Mabrouk OS,Wong JM,LeWitt TM,Paudel R,Houlden H,Kennedy RT,Levine MS,Dauer WT

更新日期：2015-06-08 00:00:00

abstract：:In the retina, synaptic transmission between photoreceptors and downstream ON-bipolar neurons (ON-BCs) is mediated by a GPCR pathway, which plays an essential role in vision. However, the mechanisms that control signal transmission at this synapse and its relevance to behavior remain poorly understood. In this study w...

journal_title：eLife

authors： Sarria I,Pahlberg J,Cao Y,Kolesnikov AV,Kefalov VJ,Sampath AP,Martemyanov KA

更新日期：2015-04-16 00:00:00

abstract：:Even when we are at rest, our spinal cords show spontaneous, yet well organised, fluctuations of activity that might reflect sensory and motor networks. ...

journal_title：eLife

authors： Eippert F,Tracey I

更新日期：2014-08-05 00:00:00

abstract：:When a human catches a ball, they estimate future target location based on the current trajectory. How animals, small and large, encode such predictive processes at the single neuron level is unknown. Here we describe small target-selective neurons in predatory dragonflies that exhibit localized enhanced sensitivity f...

journal_title：eLife

authors： Wiederman SD,Fabian JM,Dunbier JR,O'Carroll DC

更新日期：2017-07-25 00:00:00

abstract：:Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remai...

journal_title：eLife

authors： Teves SS,An L,Bhargava-Shah A,Xie L,Darzacq X,Tjian R

更新日期：2018-06-25 00:00:00

abstract：:The eukaryotic replisome assembles around the CMG helicase, which stably associates with DNA replication forks throughout elongation. When replication terminates, CMG is ubiquitylated on its Mcm7 subunit and disassembled by the Cdc48/p97 ATPase. Until now, the regulation that restricts CMG ubiquitylation to terminatio...

journal_title：eLife

authors： Deegan TD,Mukherjee PP,Fujisawa R,Polo Rivera C,Labib K

更新日期：2020-08-17 00:00:00

abstract：:Overproduction of immunoglobulin light chains leads to systemic amyloidosis, a lethal disease characterized by the formation of amyloid fibrils in patients' tissues. Excess light chains are in equilibrium between dimers and less stable monomers which can undergo irreversible aggregation to the amyloid state. The dimer...

journal_title：eLife

authors： Brumshtein B,Esswein SR,Salwinski L,Phillips ML,Ly AT,Cascio D,Sawaya MR,Eisenberg DS

更新日期：2015-11-18 00:00:00

abstract：:To elucidate new functions of sphingosine (Sph), we demonstrate that the spontaneous elevation of intracellular Sph levels via caged Sph leads to a significant and transient calcium release from acidic stores that is independent of sphingosine 1-phosphate, extracellular and ER calcium levels. This photo-induced Sph-dr...

journal_title：eLife

authors： Höglinger D,Haberkant P,Aguilera-Romero A,Riezman H,Porter FD,Platt FM,Galione A,Schultz C

更新日期：2015-11-27 00:00:00

abstract：:In bacteria, multicellular behaviors are regulated by cell-cell signaling through the exchange of both diffusible and contact-dependent signals. In a multicellular context, Myxococcus cells can share outer membrane (OM) materials by an unknown mechanism involving the traAB genes and gliding motility. Using live imagin...

journal_title：eLife

authors： Ducret A,Fleuchot B,Bergam P,Mignot T

更新日期：2013-07-23 00:00:00

abstract：:CpG islands are gene regulatory elements associated with the majority of mammalian promoters, yet how they regulate gene expression remains poorly understood. Here, we identify FBXL19 as a CpG island-binding protein in mouse embryonic stem (ES) cells and show that it associates with the CDK-Mediator complex. We discov...

journal_title：eLife

authors： Dimitrova E,Kondo T,Feldmann A,Nakayama M,Koseki Y,Konietzny R,Kessler BM,Koseki H,Klose RJ

更新日期：2018-05-29 00:00:00

abstract：:Sex-specific synaptic connectivity is beginning to emerge as a remarkable, but little explored feature of animal brains. We describe here a novel mechanism that promotes sexually dimorphic neuronal function and synaptic connectivity in the nervous system of the nematode Caenorhabditis elegans. We demonstrate that a ph...

journal_title：eLife

authors： Bayer EA,Stecky RC,Neal L,Katsamba PS,Ahlsen G,Balaji V,Hoppe T,Shapiro L,Oren-Suissa M,Hobert O

更新日期：2020-10-06 00:00:00

abstract：:Disruption of the sumoylation/desumoylation equilibrium is associated with several disease states such as cancer and infections, however the mechanisms regulating the global SUMO balance remain poorly defined. Here, we show that infection by Shigella flexneri, the causative agent of human bacillary dysentery, switches...

journal_title：eLife

authors： Lapaquette P,Fritah S,Lhocine N,Andrieux A,Nigro G,Mounier J,Sansonetti P,Dejean A

更新日期：2017-12-12 00:00:00

abstract：:Direct activation of the human phospholipase C-γ isozymes (PLC-γ1, -γ2) by tyrosine phosphorylation is fundamental to the control of diverse biological processes, including chemotaxis, platelet aggregation, and adaptive immunity. In turn, aberrant activation of PLC-γ1 and PLC-γ2 is implicated in inflammation, autoimmu...

journal_title：eLife

authors： Hajicek N,Keith NC,Siraliev-Perez E,Temple BR,Huang W,Zhang Q,Harden TK,Sondek J

更新日期：2019-12-31 00:00:00

abstract：:Cells can avoid the effects of so-called 'nonsense' mutations by several methods, including a newly discovered mechanism driven by microRNA molecules. ...

journal_title：eLife

authors： Jopling CL

更新日期：2014-09-09 00:00:00

abstract：:Human ascariasis is a major neglected tropical disease caused by the nematode Ascaris lumbricoides. We report a 296 megabase (Mb) reference-quality genome comprised of 17,902 protein-coding genes derived from a single, representative Ascaris worm. An additional 68 worms were collected from 60 human hosts in Kenyan vil...

journal_title：eLife

authors： Easton A,Gao S,Lawton SP,Bennuru S,Khan A,Dahlstrom E,Oliveira RG,Kepha S,Porcella SF,Webster J,Anderson R,Grigg ME,Davis RE,Wang J,Nutman TB

更新日期：2020-11-06 00:00:00

abstract：:Most funding agencies rely on peer review to evaluate grant applications and proposals, but research into the use of this process by funding agencies has been limited. Here we explore if two changes to the organization of peer review for proposals submitted to various funding actions by the European Union has an influ...

journal_title：eLife

authors： Pina DG,Buljan I,Hren D,Marušić A

更新日期：2021-01-13 00:00:00

abstract：:Feedback inhibitory motifs are thought to be important for pattern separation across species. How feedback circuits may implement pattern separation of biologically plausible, temporally structured input in mammals is, however, poorly understood. We have quantitatively determined key properties of netfeedback inhibiti...

journal_title：eLife

authors： Braganza O,Mueller-Komorowska D,Kelly T,Beck H

更新日期：2020-02-20 00:00:00