Abstract:
:β-Propellers arise through the amplification of a supersecondary structure element called a blade. This process produces toroids of between four and twelve repeats, which are almost always arranged sequentially in a single polypeptide chain. We found that new propellers evolve continuously by amplification from single blades. We therefore investigated whether such nascent propellers can fold as homo-oligomers before they have been fully amplified within a single chain. One- to six-bladed building blocks derived from two seven-bladed WD40 propellers yielded stable homo-oligomers with six to nine blades, depending on the size of the building block. High-resolution structures for tetramers of two blades, trimers of three blades, and dimers of four and five blades, respectively, show structurally diverse propellers and include a novel fold, highlighting the inherent flexibility of the WD40 blade. Our data support the hypothesis that subdomain-sized fragments can provide structural versatility in the evolution of new proteins.
journal_name
Elifejournal_title
eLifeauthors
Afanasieva E,Chaudhuri I,Martin J,Hertle E,Ursinus A,Alva V,Hartmann MD,Lupas ANdoi
10.7554/eLife.49853subject
Has Abstractpub_date
2019-10-15 00:00:00issn
2050-084Xpii
49853journal_volume
8pub_type
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