Attention stabilizes the shared gain of V4 populations.

abstract：:TCF/LEF factors are ancient context-dependent enhancer-binding proteins that are activated by β-catenin following Wnt signaling. They control embryonic development and adult stem cell compartments, and their dysregulation often causes cancer. β-catenin-dependent transcription relies on the NPF motif of Pygo proteins. ...

journal_title：eLife

authors： Fiedler M,Graeb M,Mieszczanek J,Rutherford TJ,Johnson CM,Bienz M

更新日期：2015-08-27 00:00:00

abstract：:Drug treatment of 3D cancer spheroids more accurately reflects in vivo therapeutic responses compared to adherent culture studies. In EGFR-mutated lung adenocarcinoma, EGFR-TKIs show enhanced efficacy in spheroid cultures. Simultaneous inhibition of multiple parallel RTKs further enhances EGFR-TKI effectiveness. We sh...

journal_title：eLife

authors： Theard PL,Sheffels E,Sealover NE,Linke AJ,Pratico DJ,Kortum RL

更新日期：2020-09-08 00:00:00

abstract：:Termination of messenger RNA translation in Bacteria and Archaea is initiated by release factors (RFs) 1 or 2 recognizing a stop codon in the ribosomal A site and releasing the peptide from the P-site transfer RNA. After release, RF-dissociation is facilitated by the G-protein RF3. Structures of ribosomal complexes wi...

journal_title：eLife

authors： Pallesen J,Hashem Y,Korkmaz G,Koripella RK,Huang C,Ehrenberg M,Sanyal S,Frank J

更新日期：2013-06-04 00:00:00

abstract：:Energy metabolism is essential for T cell function. However, how persistent antigenic stimulation affects T cell metabolism is unknown. Here, we report that long-term in vivo antigenic exposure induced a specific deficit in numerous metabolic enzymes. Accordingly, T cells exhibited low basal glycolytic flux and limite...

journal_title：eLife

authors： Bettonville M,d'Aria S,Weatherly K,Porporato PE,Zhang J,Bousbata S,Sonveaux P,Braun MY

更新日期：2018-06-18 00:00:00

abstract：:Chemosensory neurons extract information about chemical cues from the environment. How is the activity in these sensory neurons transformed into behavior? Using Caenorhabditis elegans, we map a novel sensory neuron circuit motif that encodes odor concentration. Primary neurons, AWC(ON) and AWA, directly detect the foo...

journal_title：eLife

authors： Leinwand SG,Yang CJ,Bazopoulou D,Chronis N,Srinivasan J,Chalasani SH

更新日期：2015-09-22 00:00:00

abstract：:Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease. Although rigorous efforts identified the presence of 'cancer stem cells (CSCs)' in PDAC and molecular markers for them, stem cell dynamics in vivo have not been clearly demonstrated. Here we focused on Doublecortin-like kinase 1 (Dclk1), known as a CSC ...

journal_title：eLife

authors： Maruno T,Fukuda A,Goto N,Tsuda M,Ikuta K,Hiramatsu Y,Ogawa S,Nakanishi Y,Yamaga Y,Yoshioka T,Takaori K,Uemoto S,Saur D,Chiba T,Seno H

更新日期：2021-01-04 00:00:00

abstract：:Knowledge of the host factors required for norovirus replication has been hindered by the challenges associated with culturing human noroviruses. We have combined proteomic analysis of the viral translation and replication complexes with a CRISPR screen, to identify host factors required for norovirus infection. The c...

journal_title：eLife

authors： Hosmillo M,Lu J,McAllaster MR,Eaglesham JB,Wang X,Emmott E,Domingues P,Chaudhry Y,Fitzmaurice TJ,Tung MK,Panas MD,McInerney G,Locker N,Wilen CB,Goodfellow IG

更新日期：2019-08-12 00:00:00

abstract：:Patterns of spatial positioning of individuals within microbial communities are often critical to community function. However, understanding patterning in natural communities is hampered by the multitude of cell-cell and cell-environment interactions as well as environmental variability. Here, through simulations and ...

journal_title：eLife

authors： Momeni B,Brileya KA,Fields MW,Shou W

更新日期：2013-01-22 00:00:00

abstract：:Nucleolar protein interacting with the FHA domain of pKi-67 (NIFK) is a Ki-67-interacting protein. However, its precise function in cancer remains largely uninvestigated. Here we show the clinical significance and metastatic mechanism of NIFK in lung cancer. NIFK expression is clinically associated with poor prognosis...

journal_title：eLife

authors： Lin TC,Su CY,Wu PY,Lai TC,Pan WA,Jan YH,Chang YC,Yeh CT,Chen CL,Ger LP,Chang HT,Yang CJ,Huang MS,Liu YP,Lin YF,Shyy JY,Tsai MD,Hsiao M

更新日期：2016-03-17 00:00:00

abstract：:We collated contact tracing data from COVID-19 clusters in Singapore and Tianjin, China and estimated the extent of pre-symptomatic transmission by estimating incubation periods and serial intervals. The mean incubation periods accounting for intermediate cases were 4.91 days (95%CI 4.35, 5.69) and 7.54 (95%CI 6.76, 8...

journal_title：eLife

authors： Tindale LC,Stockdale JE,Coombe M,Garlock ES,Lau WYV,Saraswat M,Zhang L,Chen D,Wallinga J,Colijn C

更新日期：2020-06-22 00:00:00

abstract：:How Mycobacterium tuberculosis (Mtb) rewires macrophage energy metabolism to facilitate survival is poorly characterized. Here, we used extracellular flux analysis to simultaneously measure the rates of glycolysis and respiration in real time. Mtb infection induced a quiescent energy phenotype in human monocyte-derive...

journal_title：eLife

authors： Cumming BM,Addicott KW,Adamson JH,Steyn AJ

更新日期：2018-11-16 00:00:00

abstract：:Systemic blood pressure is determined, in part, by arterial smooth muscle cells (myocytes). Several Transient Receptor Potential (TRP) channels are proposed to be expressed in arterial myocytes, but it is unclear if these proteins control physiological blood pressure and contribute to hypertension in vivo. We generate...

journal_title：eLife

authors： Bulley S,Fernández-Peña C,Hasan R,Leo MD,Muralidharan P,Mackay CE,Evanson KW,Moreira-Junior L,Mata-Daboin A,Burris SK,Wang Q,Kuruvilla KP,Jaggar JH

更新日期：2018-12-04 00:00:00

abstract：:Establishing how neurocircuit activation causes particular behaviors requires modulating the activity of specific neurons. Here, we demonstrate that magnetothermal genetic stimulation provides tetherless deep brain activation sufficient to evoke motor behavior in awake mice. The approach uses alternating magnetic fiel...

journal_title：eLife

authors： Munshi R,Qadri SM,Zhang Q,Castellanos Rubio I,Del Pino P,Pralle A

更新日期：2017-08-15 00:00:00

abstract：:Prion diseases are caused by PrPSc, a self-replicating pathologically misfolded protein that exerts toxicity predominantly in the brain. The administration of PrPSc causes a robust, reproducible and specific disease manifestation. Here, we have applied a combination of translating ribosome affinity purification and ri...

journal_title：eLife

authors： Scheckel C,Imeri M,Schwarz P,Aguzzi A

更新日期：2020-09-22 00:00:00

abstract：:Humans and animals construct internal models of their environment in order to select appropriate courses of action. The representation of uncertainty about the current state of the environment is a key feature of these models that controls the rate of learning as well as directly affecting choice behaviour. To maintai...

journal_title：eLife

authors： Muller TH,Mars RB,Behrens TE,O'Reilly JX

更新日期：2019-02-28 00:00:00

abstract：:Sudden changes in the level of a coenzyme called NADPH might be the underlying cause of aging in cells. ...

journal_title：eLife

authors： Barral Y

更新日期：2013-02-05 00:00:00

abstract：:Bioluminescence is a powerful biological signal that scientists have repurposed as a reporter for gene expression in plants and animals. However, there are downsides associated with the need to provide a substrate to these reporters, including its high cost and non-uniform tissue penetration. In this work we reconstit...

journal_title：eLife

authors： Khakhar A,Starker CG,Chamness JC,Lee N,Stokke S,Wang C,Swanson R,Rizvi F,Imaizumi T,Voytas DF

更新日期：2020-03-25 00:00:00

abstract：:In many plant species, a subset of transcribed genes are characterized by strictly CG-context DNA methylation, referred to as gene body methylation (gbM). The mechanisms that establish gbM are unclear, yet flowering plant species naturally without gbM lack the DNA methyltransferase, CMT3, which maintains CHG (H = A, C...

journal_title：eLife

authors： Wendte JM,Zhang Y,Ji L,Shi X,Hazarika RR,Shahryary Y,Johannes F,Schmitz RJ

更新日期：2019-07-29 00:00:00

abstract：:Though neurotransmitters are essential elements in neuronal signal transduction, techniques for in vivo analysis are still limited. Here, we describe an organic electrochemical transistor array (OECT-array) technique for monitoring catecholamine neurotransmitters (CA-NTs) in rat brains. The OECT-array is an active sen...

journal_title：eLife

authors： Xie K,Wang N,Lin X,Wang Z,Zhao X,Fang P,Yue H,Kim J,Luo J,Cui S,Yan F,Shi P

更新日期：2020-02-11 00:00:00

abstract：:Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remai...

journal_title：eLife

authors： Teves SS,An L,Bhargava-Shah A,Xie L,Darzacq X,Tjian R

更新日期：2018-06-25 00:00:00

abstract：:The structure of fitness landscapes is critical for understanding adaptive protein evolution. Previous empirical studies on fitness landscapes were confined to either the neighborhood around the wild type sequence, involving mostly single and double mutants, or a combinatorially complete subgraph involving only two am...

journal_title：eLife

authors： Wu NC,Dai L,Olson CA,Lloyd-Smith JO,Sun R

更新日期：2016-07-08 00:00:00

abstract：:The circadian transcriptional repressors cryptochrome 1 (Cry1) and 2 (Cry2) evolved from photolyases, bacterial light-activated DNA repair enzymes. In this study, we report that while they have lost DNA repair activity, Cry1/2 adapted to protect genomic integrity by responding to DNA damage through posttranslational m...

journal_title：eLife

authors： Papp SJ,Huber AL,Jordan SD,Kriebs A,Nguyen M,Moresco JJ,Yates JR,Lamia KA

更新日期：2015-03-10 00:00:00

abstract：:Complexin regulates spontaneous and activates Ca(2+)-triggered neurotransmitter release, yet the molecular mechanisms are still unclear. Here we performed single molecule fluorescence resonance energy transfer experiments and uncovered two conformations of complexin-1 bound to the ternary SNARE complex. In the cis con...

journal_title：eLife

authors： Choi UB,Zhao M,Zhang Y,Lai Y,Brunger AT

更新日期：2016-06-02 00:00:00

abstract：:Intermittent hypoxia (IH) is the predominant pathophysiological disturbance in obstructive sleep apnea (OSA), known to be independently associated with cardiovascular diseases. However, the effect of IH on cardiac fibrosis and molecular events involved in this process are unclear. Here, we tested IH in angiotensin II ...

journal_title：eLife

authors： Bao Q,Zhang B,Suo Y,Liu C,Yang Q,Zhang K,Yuan M,Yuan M,Zhang Y,Li G

更新日期：2020-01-14 00:00:00

abstract：:Plasticity can put evolution on repeat if development causes species to generate similar morphologies in similar environments. Anolis lizards offer the opportunity to put this role of developmental plasticity to the test. Following colonization of the four Greater Antillean islands, Anolis lizards independently and re...

journal_title：eLife

authors： Feiner N,Jackson IS,Munch KL,Radersma R,Uller T

更新日期：2020-08-13 00:00:00

abstract：:Skeletal muscle degeneration is a complication arising from a variety of chronic diseases including advanced cancer. Pro-inflammatory cytokine TNF-α plays a pivotal role in mediating cancer-related skeletal muscle degeneration. Here, we show a novel function for retinoblastoma protein (Rb), where Rb causes sarcomeric ...

journal_title：eLife

authors： Araki K,Kawauchi K,Hirata H,Yamamoto M,Taya Y

更新日期：2013-12-03 00:00:00

abstract：:Voltage-gated sodium channels are targets for a range of pharmaceutical drugs developed for the treatment of neurological diseases. Cannabidiol (CBD), the non-psychoactive compound isolated from cannabis plants, was recently approved for treatment of two types of epilepsy associated with sodium channel mutations. This...

journal_title：eLife

authors： Sait LG,Sula A,Ghovanloo MR,Hollingworth D,Ruben PC,Wallace BA

更新日期：2020-10-22 00:00:00

abstract：:Most evolutionary thinking is based on the notion of fitness and related ideas such as fitness landscapes and evolutionary optima. Nevertheless, it is often unclear what fitness actually is, and its meaning often depends on the context. Here we argue that fitness should not be a basal ingredient in verbal or mathemati...

journal_title：eLife

authors： Doebeli M,Ispolatov Y,Simon B

更新日期：2017-02-15 00:00:00

abstract：:Microbes are embedded in complex communities where they engage in a wide array of intra- and inter-specific interactions. The extent to which these interactions drive or impede microbiome diversity is not well understood. Historically, two contrasting hypotheses have been suggested to explain how species interactions ...

journal_title：eLife

authors： Madi N,Vos M,Murall CL,Legendre P,Shapiro BJ

更新日期：2020-11-20 00:00:00

abstract：:Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans after silicone oil (S...

journal_title：eLife

authors： Zhang J,Li L,Huang H,Fang F,Webber HC,Zhuang P,Liu L,Dalal R,Tang PH,Mahajan VB,Sun Y,Li S,Zhang M,Goldberg JL,Hu Y

更新日期：2019-05-15 00:00:00