The dyskerin ribonucleoprotein complex as an OCT4/SOX2 coactivator in embryonic stem cells.

abstract：:The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...

journal_title：eLife

authors： Lischinsky JE,Sokolowski K,Li P,Esumi S,Kamal Y,Goodrich M,Oboti L,Hammond TR,Krishnamoorthy M,Feldman D,Huntsman M,Liu J,Corbin JG

更新日期：2017-03-13 00:00:00

abstract：:Bone destruction relies on interactions between bone and immune cells. Bone-resorbing osteoclasts (OCLs) were recently identified as innate immune cells activating T cells toward tolerance or inflammation. Thus, pathological bone destruction not only relies on increased osteoclast differentiation, but also on the pres...

journal_title：eLife

authors： Madel MB,Ibáñez L,Ciucci T,Halper J,Rouleau M,Boutin A,Hue C,Duroux-Richard I,Apparailly F,Garchon HJ,Wakkach A,Blin-Wakkach C

更新日期：2020-05-13 00:00:00

abstract：:Most funding agencies rely on peer review to evaluate grant applications and proposals, but research into the use of this process by funding agencies has been limited. Here we explore if two changes to the organization of peer review for proposals submitted to various funding actions by the European Union has an influ...

journal_title：eLife

authors： Pina DG,Buljan I,Hren D,Marušić A

更新日期：2021-01-13 00:00:00

abstract：:Actin, spectrin, and associated molecules form a periodic sub-membrane lattice structure in axons. How this membrane skeleton is developed and why it preferentially forms in axons are unknown. Here, we studied the developmental mechanism of this lattice structure. We found that this structure emerged early during axon...

journal_title：eLife

authors： Zhong G,He J,Zhou R,Lorenzo D,Babcock HP,Bennett V,Zhuang X

更新日期：2014-12-23 00:00:00

abstract：:Selective packaging of HIV-1 genomic RNA (gRNA) requires the presence of a cis-acting RNA element called the 'packaging signal' (Ψ). However, the mechanism by which Ψ promotes selective packaging of the gRNA is not well understood. We used fluorescence correlation spectroscopy and quenching data to monitor the binding...

journal_title：eLife

authors： Comas-Garcia M,Datta SA,Baker L,Varma R,Gudla PR,Rein A

更新日期：2017-07-20 00:00:00

abstract：:Binary fission has been well studied in rod-shaped bacteria, but the mechanisms underlying cell division in spherical bacteria are poorly understood. Rod-shaped bacteria harbor regulatory proteins that place and remodel the division machinery during cytokinesis. In the spherical human pathogen Staphylococcus aureus, w...

journal_title：eLife

authors： Eswara PJ,Brzozowski RS,Viola MG,Graham G,Spanoudis C,Trebino C,Jha J,Aubee JI,Thompson KM,Camberg JL,Ramamurthi KS

更新日期：2018-10-02 00:00:00

abstract：:We discovered that Enterococcus faecium (E. faecium), a ubiquitous commensal bacterium, and its secreted peptidoglycan hydrolase (SagA) were sufficient to enhance intestinal barrier function and pathogen tolerance, but the precise biochemical mechanism was unknown. Here we show E. faecium has unique peptidoglycan comp...

journal_title：eLife

authors： Kim B,Wang YC,Hespen CW,Espinosa J,Salje J,Rangan KJ,Oren DA,Kang JY,Pedicord VA,Hang HC

更新日期：2019-04-10 00:00:00

abstract：:Secondary metabolites have a great potential as pharmaceuticals, but there are only a few examples where regulation of gene cluster expression has been correlated with ecological and physiological relevance for the producer. Here, signals, mediators, and biological effects of terrein production were studied in the fun...

journal_title：eLife

authors： Gressler M,Meyer F,Heine D,Hortschansky P,Hertweck C,Brock M

更新日期：2015-07-14 00:00:00

abstract：:TMEM16A is a ligand-gated anion channel that is activated by intracellular Ca2+. This channel comprises two independent pores and closely apposed Ca2+ binding sites that are contained within each subunit of a homodimeric protein. Previously we characterized the influence of positively charged pore-lining residues on a...

journal_title：eLife

authors： Lam AK,Dutzler R

更新日期：2018-10-12 00:00:00

abstract：:The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucleosome, constrains t...

journal_title：eLife

authors： Isaac RS,Jiang F,Doudna JA,Lim WA,Narlikar GJ,Almeida R

更新日期：2016-04-28 00:00:00

abstract：:The inferior colliculus (IC) is the major midbrain auditory integration center, where virtually all ascending auditory inputs converge. Although the IC has been extensively studied for sound processing, little is known about the neural activity of the IC in moving subjects, as frequently happens in natural hearing con...

journal_title：eLife

authors： Yang Y,Lee J,Kim G

更新日期：2020-01-28 00:00:00

abstract：:In the developing fruit fly brain, a protein called Trithorax increases the number of neural cells produced from a single stem cell, in part by regulating the transcription of the target genes buttonhead and pointed. ...

journal_title：eLife

authors： Jiang Y,Reichert H

更新日期：2014-11-06 00:00:00

abstract：:Two recently discovered transcription factors stop cells from dividing when plants face extreme heat and DNA damage. ...

journal_title：eLife

authors： Eekhout T,De Veylder L

更新日期：2019-04-09 00:00:00

abstract：:The training of PhD students and early-career scientists is largely an apprenticeship in which the trainee associates with an expert to become an independent scientist. But when is a PhD student ready to graduate, a postdoctoral scholar ready for an independent position, or an early-career scientist ready for advanced...

journal_title：eLife

authors： Verderame MF,Freedman VH,Kozlowski LM,McCormack WT

更新日期：2018-05-31 00:00:00

abstract：:Numerous brain regions have been shown to have neural correlates of gradually accumulating evidence for decision-making, but the causal roles of these regions in decisions driven by accumulation of evidence have yet to be determined. Here, in rats performing an auditory evidence accumulation task, we inactivated the f...

journal_title：eLife

authors： Erlich JC,Brunton BW,Duan CA,Hanks TD,Brody CD

更新日期：2015-04-14 00:00:00

abstract：:Genome sequence data from a range of animal species are raising questions about the origins of glutamate receptors. ...

journal_title：eLife

authors： Mayer ML,Jegla T

更新日期：2018-12-28 00:00:00

abstract：:A hallmark of CRISPR-Cas immunity systems is the CRISPR array, a genomic locus consisting of short, repeated sequences ('repeats') interspersed with short, variable sequences ('spacers'). CRISPR arrays are transcribed and processed into individual CRISPR RNAs that each include a single spacer, and direct Cas proteins ...

journal_title：eLife

authors： Stringer AM,Baniulyte G,Lasek-Nesselquist E,Seed KD,Wade JT

更新日期：2020-10-30 00:00:00

abstract：:Rod and cone photoreceptors are highly similar in many respects but they have important functional and molecular differences. Here, we investigate genome-wide patterns of DNA methylation and chromatin accessibility in mouse rods and cones and correlate differences in these features with gene expression, histone marks,...

journal_title：eLife

authors： Mo A,Luo C,Davis FP,Mukamel EA,Henry GL,Nery JR,Urich MA,Picard S,Lister R,Eddy SR,Beer MA,Ecker JR,Nathans J

更新日期：2016-03-07 00:00:00

abstract：:In 2015, as part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Kandela et al., 2015) that described how we intended to replicate selected experiments from the paper "BET bromodomain inhibition as a therapeutic strategy to target c-Myc" (Delmore et al., 2011). Here we report the resu...

journal_title：eLife

authors： Aird F,Kandela I,Mantis C,Reproducibility Project: Cancer Biology.

更新日期：2017-01-19 00:00:00

abstract：:Genome-wide association studies identified the BIN1 locus as a leading modulator of genetic risk in Alzheimer's disease (AD). One limitation in understanding BIN1's contribution to AD is its unknown function in the brain. AD-associated BIN1 variants are generally noncoding and likely change expression. Here, we determ...

journal_title：eLife

authors： Voskobiynyk Y,Roth JR,Cochran JN,Rush T,Carullo NV,Mesina JS,Waqas M,Vollmer RM,Day JJ,McMahon LL,Roberson ED

更新日期：2020-07-13 00:00:00

abstract：:Postsynaptic compartments can be specifically modulated during various forms of synaptic plasticity, but it is unclear whether this precision is shared at presynaptic terminals. Presynaptic homeostatic plasticity (PHP) stabilizes neurotransmission at the Drosophila neuromuscular junction, where a retrograde enhancemen...

journal_title：eLife

authors： Li X,Goel P,Chen C,Angajala V,Chen X,Dickman DK

更新日期：2018-04-05 00:00:00

abstract：:Comprehensively elucidating the molecular mechanisms of human immunodeficiency virus type 1 (HIV-1) latency is a priority to achieve a functional cure. As current 'shock' agents failed to efficiently reactivate the latent reservoir, it is important to discover new targets for developing more efficient latency-reversin...

journal_title：eLife

authors： Ma X,Yang T,Luo Y,Wu L,Jiang Y,Song Z,Pan T,Liu B,Liu G,Liu J,Yu F,He Z,Zhang W,Yang J,Liang L,Guan Y,Zhang X,Li L,Cai W,Tang X,Gao S,Deng K,Zhang H

更新日期：2019-01-17 00:00:00

abstract：:Exhaustion of stem cells is a hallmark of aging. In the Drosophila testis, dedifferentiated germline stem cells (GSCs) derived from spermatogonia increase during lifespan, leading to the model that dedifferentiation counteracts the decline of GSCs in aged males. To test this, we blocked dedifferentiation by mis-expres...

journal_title：eLife

authors： Herrera SC,Bach EA

更新日期：2018-07-09 00:00:00

abstract：:Myelin destruction is followed by resident glia activation and mobilization of endogenous progenitors (OPC) which participate in myelin repair. Here we show that in response to demyelination, mature oligodendrocytes (OLG) bordering the lesion express Ndst1, a key enzyme for heparan sulfates (HS) synthesis. Ndst1+ OLG ...

journal_title：eLife

authors： Macchi M,Magalon K,Zimmer C,Peeva E,El Waly B,Brousse B,Jaekel S,Grobe K,Kiefer F,Williams A,Cayre M,Durbec P

更新日期：2020-06-09 00:00:00

abstract：:The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here, we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our ...

journal_title：eLife

authors： Cong L,Wang Z,Chai Y,Hang W,Shang C,Yang W,Bai L,Du J,Wang K,Wen Q

更新日期：2017-09-20 00:00:00

abstract：:A new source of progenitor cells can now be used to study hidden aspects of human lung development and pediatric lung disease. ...

journal_title：eLife

authors： Waghray A,Rajagopal J

更新日期：2017-08-14 00:00:00

abstract：:Wnt plays important role during development and in various diseases. Because Wnts are lipidated and highly hydrophobic, they can only be purified in the presence of detergents, limiting their use in various in vitro and in vivo assays. We purified N-terminally tagged recombinant Wnt3a secreted from cells and accidenta...

journal_title：eLife

authors： Mihara E,Hirai H,Yamamoto H,Tamura-Kawakami K,Matano M,Kikuchi A,Sato T,Takagi J

更新日期：2016-02-23 00:00:00

abstract：:Unfavorable redox conditions in the endoplasmic reticulum (ER) can decrease the capacity for protein secretion, altering vital cell functions. While systems to manage reductive stress are well-established, how cells cope with an overly oxidizing ER remains largely undefined. In previous work (Wang et al., 2014), we de...

journal_title：eLife

authors： Siegenthaler KD,Pareja KA,Wang J,Sevier CS

更新日期：2017-03-03 00:00:00

abstract：:The investigation of prosocial behavior is of particular interest from an evolutionary perspective. Comparisons of prosociality across non-human animal species have, however, so far largely focused on primates, and their interpretation is hampered by the diversity of paradigms and procedures used. Here, we present the...

journal_title：eLife

authors： Horn L,Bugnyar T,Griesser M,Hengl M,Izawa EI,Oortwijn T,Rössler C,Scheer C,Schiestl M,Suyama M,Taylor AH,Vanhooland LC,von Bayern AM,Zürcher Y,Massen JJ

更新日期：2020-10-20 00:00:00

abstract：:Cells use molecular circuits to interpret and respond to extracellular cues, such as hormones and cytokines, which are often released in a temporally varying fashion. In this study, we combine microfluidics, time-lapse microscopy, and computational modeling to investigate how the type I interferon (IFN)-responsive reg...

journal_title：eLife

authors： Mudla A,Jiang Y,Arimoto KI,Xu B,Rajesh A,Ryan AP,Wang W,Daugherty MD,Zhang DE,Hao N

更新日期：2020-09-18 00:00:00