Abstract:
:Postsynaptic compartments can be specifically modulated during various forms of synaptic plasticity, but it is unclear whether this precision is shared at presynaptic terminals. Presynaptic homeostatic plasticity (PHP) stabilizes neurotransmission at the Drosophila neuromuscular junction, where a retrograde enhancement of presynaptic neurotransmitter release compensates for diminished postsynaptic receptor functionality. To test the specificity of PHP induction and expression, we have developed a genetic manipulation to reduce postsynaptic receptor expression at one of the two muscles innervated by a single motor neuron. We find that PHP can be induced and expressed at a subset of synapses, over both acute and chronic time scales, without influencing transmission at adjacent release sites. Further, homeostatic modulations to CaMKII, vesicle pools, and functional release sites are compartmentalized and do not spread to neighboring pre- or post-synaptic structures. Thus, both PHP induction and expression mechanisms are locally transmitted and restricted to specific synaptic compartments.
journal_name
Elifejournal_title
eLifeauthors
Li X,Goel P,Chen C,Angajala V,Chen X,Dickman DKdoi
10.7554/eLife.34338subject
Has Abstractpub_date
2018-04-05 00:00:00issn
2050-084Xpii
34338journal_volume
7pub_type
杂志文章相关文献
eLife文献大全abstract::Our understanding of the antigen presentation pathway has recently been enhanced with the identification that the tapasin-related protein TAPBPR is a second major histocompatibility complex (MHC) class I-specific chaperone. We sought to determine whether, like tapasin, TAPBPR can also influence MHC class I peptide sel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.09617
更新日期:2015-10-06 00:00:00
abstract::The Hippo-YAP pathway is a central regulator of cell contact inhibition, proliferation and death. There are conflicting reports regarding the role of Angiomotin (Amot) in regulating this pathway. While some studies suggest a YAP-inhibitory function other studies indicate Amot is required for YAP activity. Here, we des...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23966
更新日期:2017-05-03 00:00:00
abstract::In most vertebrate neurons, action potentials are triggered at the distal end of the axon initial segment (AIS). Both position and length of the AIS vary across and within neuron types, with activity, development and pathology. What is the impact of AIS geometry on excitability? Direct empirical assessment has proven ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53432
更新日期:2020-03-30 00:00:00
abstract::While host immune receptors detect pathogen-associated molecular patterns to activate immunity, pathogens attempt to deregulate host immunity through secreted effectors. Fungi employ LysM effectors to prevent recognition of cell wall-derived chitin by host immune receptors, although the mechanism to compete for chitin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00790
更新日期:2013-07-02 00:00:00
abstract::Antiviral effectors such as natural killer (NK) cells have impaired functions in chronic hepatitis B (CHB) patients. The molecular mechanism responsible for this dysfunction remains poorly characterised. We show that decreased cytokine production capacity of peripheral NK cells from CHB patients was associated with re...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60095
更新日期:2021-01-28 00:00:00
abstract::Voltage-gated sodium channels are targets for a range of pharmaceutical drugs developed for the treatment of neurological diseases. Cannabidiol (CBD), the non-psychoactive compound isolated from cannabis plants, was recently approved for treatment of two types of epilepsy associated with sodium channel mutations. This...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58593
更新日期:2020-10-22 00:00:00
abstract::Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional equivalents across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07410
更新日期:2015-05-29 00:00:00
abstract:: ...
journal_title:eLife
pub_type: 已发布勘误
doi:10.7554/eLife.66683
更新日期:2021-01-25 00:00:00
abstract::Cells can avoid the effects of so-called 'nonsense' mutations by several methods, including a newly discovered mechanism driven by microRNA molecules. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.04300
更新日期:2014-09-09 00:00:00
abstract::A transcription factor that regulates skeleton formation in sea urchin embryos has evolved a new domain that is essential for this process. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.33792
更新日期:2018-01-08 00:00:00
abstract::Homologous recombination is widespread and catalyzes evolution. Nonetheless, its existence in animal mitochondrial DNA is questioned. We designed selections for recombination between co-resident mitochondrial genomes in various heteroplasmic Drosophila lines. In four experimental settings, recombinant genomes became t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07247
更新日期:2015-08-03 00:00:00
abstract::Synaptic adhesion molecules regulate synapse development and function. However, whether and how presynaptic adhesion molecules regulate postsynaptic NMDAR function remains largely unclear. Presynaptic LAR family receptor tyrosine phosphatases (LAR-RPTPs) regulate synapse development through mechanisms that include tra...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54224
更新日期:2020-03-06 00:00:00
abstract::Rising and more variable global temperatures pose a challenge for biodiversity, with reproduction and fertility being especially sensitive to heat. Here, we assessed the potential for thermal adaptation in sperm and egg function using Tribolium flour beetles, a warm-temperate-tropical insect model. Following temperatu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49452
更新日期:2019-10-01 00:00:00
abstract::During mitosis chromosomes reorganise into highly compact, rod-shaped forms, thought to consist of consecutive chromatin loops around a central protein scaffold. Condensin complexes are involved in chromatin compaction, but the contribution of other chromatin proteins, DNA sequence and histone modifications is less un...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57212
更新日期:2020-09-11 00:00:00
abstract::In hypoxic stress conditions, glycolysis enzymes assemble into singular cytoplasmic granules called glycolytic (G) bodies. G body formation in yeast correlates with increased glucose consumption and cell survival. However, the physical properties and organizing principles that define G body formation are unclear. We d...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.48480
更新日期:2020-04-16 00:00:00
abstract::Disruption of the sumoylation/desumoylation equilibrium is associated with several disease states such as cancer and infections, however the mechanisms regulating the global SUMO balance remain poorly defined. Here, we show that infection by Shigella flexneri, the causative agent of human bacillary dysentery, switches...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27444
更新日期:2017-12-12 00:00:00
abstract::Hepatitis B virus (HBV) is an important but difficult to study human pathogen. Most basics of the hepadnaviral life-cycle were unraveled using duck HBV (DHBV) as a model although DHBV has a capsid protein (CP) comprising ~260 rather than ~180 amino acids. Here we present high-resolution structures of several DHBV caps...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57277
更新日期:2020-08-14 00:00:00
abstract::Viral replicase recruitment and long-range RNA interactions are essential for RNA virus replication, yet the mechanism of their interplay remains elusive. Flaviviruses include numerous important human pathogens, e.g., dengue virus (DENV) and Zika virus (ZIKV). Here, we revealed a highly conserved, conformation-tunable...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17636
更新日期:2016-10-01 00:00:00
abstract::Clinical trials are currently underway to assess the efficacy of forniceal deep brain stimulation (DBS) for improvement of memory in Alzheimer's patients, and forniceal DBS has been shown to improve learning and memory in a mouse model of Rett syndrome (RTT), an intellectual disability disorder caused by loss-of-funct...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.34031
更新日期:2018-03-23 00:00:00
abstract::The human insular cortex is a heterogeneous brain structure which plays an integrative role in guiding behavior. The cytoarchitectonic organization of the human insula has been investigated over the last century using postmortem brains but there has been little progress in noninvasive in vivo mapping of its microstruc...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53470
更新日期:2020-06-04 00:00:00
abstract::Selective packaging of HIV-1 genomic RNA (gRNA) requires the presence of a cis-acting RNA element called the 'packaging signal' (Ψ). However, the mechanism by which Ψ promotes selective packaging of the gRNA is not well understood. We used fluorescence correlation spectroscopy and quenching data to monitor the binding...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27055
更新日期:2017-07-20 00:00:00
abstract::Asymmetric disassembly of the synaptonemal complex (SC) is crucial for proper meiotic chromosome segregation. However, the signaling mechanisms that directly regulate this process are poorly understood. Here we show that the mammalian Rho GEF homolog, ECT-2, functions through the conserved RAS/ERK MAP kinase signaling...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12039
更新日期:2016-02-27 00:00:00
abstract::Systemic oxygen restriction (SOR) is prevalent in numerous clinical conditions, including chronic obstructive pulmonary disease (COPD), and is associated with increased susceptibility to viral infections. However, the influence of SOR on T cell immunity remains uncharacterized. Here we show the detrimental effect of h...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56612
更新日期:2020-11-23 00:00:00
abstract::Development of specialized cells and structures in the heart is regulated by spatially -restricted molecular pathways. Disruptions in these pathways can cause severe congenital cardiac malformations or functional defects. To better understand these pathways and how they regulate cardiac development we used tomo-seq, c...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.31515
更新日期:2018-02-05 00:00:00
abstract::In 2015, as part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Kandela et al., 2015) that described how we intended to replicate selected experiments from the paper "BET bromodomain inhibition as a therapeutic strategy to target c-Myc" (Delmore et al., 2011). Here we report the resu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21253
更新日期:2017-01-19 00:00:00
abstract::Most funding agencies rely on peer review to evaluate grant applications and proposals, but research into the use of this process by funding agencies has been limited. Here we explore if two changes to the organization of peer review for proposals submitted to various funding actions by the European Union has an influ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59338
更新日期:2021-01-13 00:00:00
abstract::CRISPR-based homing gene drives have sparked both enthusiasm and deep concerns due to their potential for genetically altering entire species. This raises the question about our ability to prevent the unintended spread of such drives from the laboratory into a natural population. Here, we experimentally demonstrate th...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41439
更新日期:2019-01-22 00:00:00
abstract::Multicellular development is driven by regulatory programs that orchestrate the transcription of protein-coding and noncoding genes. To decipher this genomic regulatory code, and to investigate the developmental relevance of noncoding transcription, we compared genome-wide promoter activity throughout embryogenesis in...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.29005
更新日期:2017-12-20 00:00:00
abstract::Here, we describe the case of a COVID-19 patient who developed recurring ventilator-associated pneumonia caused by Pseudomonas aeruginosa that acquired increasing levels of antimicrobial resistance (AMR) in response to treatment. Metagenomic analysis revealed the AMR genotype, while immunological analysis revealed mas...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.63430
更新日期:2020-12-17 00:00:00
abstract::A heat exchange mechanism in the head of kissing bugs helps to prevent stress and regulate their temperature while they feed on warm blood. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.33035
更新日期:2017-11-21 00:00:00