Molecular safeguarding of CRISPR gene drive experiments.

abstract：:How living systems break symmetry in an organized manner is a fundamental question in biology. In wild-type Caenorhabditis elegans zygotes, symmetry breaking during anterior-posterior axis specification is guided by centrosomes, resulting in anterior-directed cortical flows and a single posterior PAR-2 domain. We unco...

journal_title：eLife

authors： Klinkert K,Levernier N,Gross P,Gentili C,von Tobel L,Pierron M,Busso C,Herrman S,Grill SW,Kruse K,Gönczy P

更新日期：2019-02-26 00:00:00

abstract：:The vascular pathogen Verticillium dahliae infects the roots of plants to cause Verticillium wilt. The molecular mechanisms underlying V. dahliae virulence and host resistance remain elusive. Here, we demonstrate that a secretory protein, VdSCP41, functions as an intracellular effector that promotes V. dahliae virulen...

journal_title：eLife

authors： Qin J,Wang K,Sun L,Xing H,Wang S,Li L,Chen S,Guo HS,Zhang J

更新日期：2018-05-14 00:00:00

abstract：:New data on the relationship between two proteins, cyclin D and Rb, suggest that we need to re-evaluate our understanding of how cells enter into the cell cycle. ...

journal_title：eLife

authors： Bertoli C,de Bruin RA

更新日期：2014-07-01 00:00:00

abstract：:This study examined records of 2566 consecutive COVID-19 patients at five Massachusetts hospitals and sought to predict level-of-care requirements based on clinical and laboratory data. Several classification methods were applied and compared against standard pneumonia severity scores. The need for hospitalization, IC...

journal_title：eLife

authors： Hao B,Sotudian S,Wang T,Xu T,Hu Y,Gaitanidis A,Breen K,Velmahos GC,Paschalidis IC

更新日期：2020-10-12 00:00:00

abstract：:SUMO1-conjugation of proteins at neuronal synapses is considered to be a major post-translational regulatory process in nerve cell and synapse function, but the published evidence for SUMO1-conjugation at synapses is contradictory. We employed multiple genetic mouse models for stringently controlled biochemical and im...

journal_title：eLife

authors： Daniel JA,Cooper BH,Palvimo JJ,Zhang FP,Brose N,Tirard M

更新日期：2017-06-09 00:00:00

abstract：:The restoration of homeostasis after tissue damage relies on proper spatial-temporal control of damage-induced apoptosis and compensatory proliferation. In Drosophila imaginal discs these processes are coordinated by the stress response pathway JNK. We demonstrate that JNK signaling induces a dose-dependent extension ...

journal_title：eLife

authors： Cosolo A,Jaiswal J,Csordás G,Grass I,Uhlirova M,Classen AK

更新日期：2019-02-08 00:00:00

abstract：:Cell-cell communication in multicellular organisms depends on the dynamic and reversible phosphorylation of protein tyrosine residues. The receptor-linked protein tyrosine phosphatases (RPTPs) receive cues from the extracellular environment and are well placed to influence cell signaling. However, the direct events do...

journal_title：eLife

authors： Fearnley GW,Young KA,Edgar JR,Antrobus R,Hay IM,Liang WC,Martinez-Martin N,Lin W,Deane JE,Sharpe HJ

更新日期：2019-03-29 00:00:00

abstract：:Hybrid sterility is one of the earliest postzygotic isolating mechanisms to evolve between two recently diverged species. Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha, which mate to form viable hybrid diploids that effici...

journal_title：eLife

authors： Zanders SE,Eickbush MT,Yu JS,Kang JW,Fowler KR,Smith GR,Malik HS

更新日期：2014-06-24 00:00:00

abstract：:Experiments in which monkeys have to select or estimate the location of a target are revealing more about the role of the dorsal premotor cortex in decision making. ...

journal_title：eLife

authors： Stuphorn V

更新日期：2016-07-15 00:00:00

abstract：:Rhythmic actions benefit from synchronization with external events. Auditory-paced finger tapping studies indicate the two cerebral hemispheres preferentially control different rhythms. It is unclear whether left-lateralized processing of faster rhythms and right-lateralized processing of slower rhythms bases upon hem...

journal_title：eLife

authors： Pflug A,Gompf F,Muthuraman M,Groppa S,Kell CA

更新日期：2019-11-07 00:00:00

abstract：:Myelin, made by oligodendrocytes, is essential for rapid information transfer in the central nervous system. Oligodendrocyte precursors (OPs) receive glutamatergic synaptic input from axons but how this affects their development is unclear. Murine OPs in white matter express AMPA receptor (AMPAR) subunits GluA2, GluA3...

journal_title：eLife

authors： Kougioumtzidou E,Shimizu T,Hamilton NB,Tohyama K,Sprengel R,Monyer H,Attwell D,Richardson WD

更新日期：2017-06-13 00:00:00

abstract：:Entry and extrusion of cations are essential processes in living cells. In alkaliphilic prokaryotes, high external pH activates voltage-gated sodium channels (Nav), which allows Na(+) to enter and be used as substrate for cation/proton antiporters responsible for cytoplasmic pH homeostasis. Here, we describe a new mem...

journal_title：eLife

authors： DeCaen PG,Takahashi Y,Krulwich TA,Ito M,Clapham DE

更新日期：2014-11-11 00:00:00

abstract：:Beige/brite adipocytes are induced within white adipose tissues (WAT) and, when activated, consume glucose and fatty acids to produce heat. Classically, two stimuli have been used to trigger a beiging response: cold temperatures and β3-adrenergic receptor (Adrb3) agonists. These two beiging triggers have been used int...

journal_title：eLife

authors： Jiang Y,Berry DC,Graff JM

更新日期：2017-10-11 00:00:00

abstract：:Most evolutionary thinking is based on the notion of fitness and related ideas such as fitness landscapes and evolutionary optima. Nevertheless, it is often unclear what fitness actually is, and its meaning often depends on the context. Here we argue that fitness should not be a basal ingredient in verbal or mathemati...

journal_title：eLife

authors： Doebeli M,Ispolatov Y,Simon B

更新日期：2017-02-15 00:00:00

abstract：:The connection between gene loss and the functional adaptation of retained proteins is still poorly understood. We apply phylogenomics and metabolic modeling to detect bacterial species that are evolving by gene loss, with the finding that Actinomycetaceae genomes from human cavities are undergoing sizable reductions,...

journal_title：eLife

authors： Juárez-Vázquez AL,Edirisinghe JN,Verduzco-Castro EA,Michalska K,Wu C,Noda-García L,Babnigg G,Endres M,Medina-Ruíz S,Santoyo-Flores J,Carrillo-Tripp M,Ton-That H,Joachimiak A,Henry CS,Barona-Gómez F

更新日期：2017-03-31 00:00:00

abstract：:Mitochondria support synaptic transmission through production of ATP, sequestration of calcium, synthesis of glutamate, and other vital functions. Surprisingly, less than 50% of hippocampal CA1 presynaptic boutons contain mitochondria, raising the question of whether synapses without mitochondria can sustain changes i...

journal_title：eLife

authors： Smith HL,Bourne JN,Cao G,Chirillo MA,Ostroff LE,Watson DJ,Harris KM

更新日期：2016-12-19 00:00:00

abstract：:Hippo signalling integrates diverse stimuli related to epithelial architecture to regulate tissue growth and cell fate decisions. The Hippo kinase cascade represses the growth-promoting transcription co-activator Yorkie. The FERM protein Expanded is one of the main upstream Hippo signalling regulators in Drosophila as...

journal_title：eLife

authors： Fulford AD,Holder MV,Frith D,Snijders AP,Tapon N,Ribeiro PS

更新日期：2019-09-30 00:00:00

abstract：:Auditory processing depends upon inhibitory signaling by interneurons, even at its earliest stages in the ventral cochlear nucleus (VCN). Remarkably, to date only a single subtype of inhibitory neuron has been documented in the VCN, a projection neuron termed the D-stellate cell. With the use of a transgenic mouse lin...

journal_title：eLife

authors： Ngodup T,Romero GE,Trussell LO

更新日期：2020-11-03 00:00:00

abstract：:Periodontal disease is an age-associated disorder clinically defined by periodontal bone loss, inflammation of the specialized tissues that surround and support the tooth, and microbiome dysbiosis. Currently, there is no therapy for reversing periodontal disease, and treatment is generally restricted to preventive mea...

journal_title：eLife

authors： An JY,Kerns KA,Ouellette A,Robinson L,Morris HD,Kaczorowski C,Park SI,Mekvanich T,Kang A,McLean JS,Cox TC,Kaeberlein M

更新日期：2020-04-28 00:00:00

abstract：:Two recently discovered transcription factors stop cells from dividing when plants face extreme heat and DNA damage. ...

journal_title：eLife

authors： Eekhout T,De Veylder L

更新日期：2019-04-09 00:00:00

abstract：:Experiments on flies suggest that a gain-of-function mechanism in a protein called CSPɑ contributes to the progressive brain disease CLN4. ...

journal_title：eLife

authors： Berryer MH,Kosmaczewski SG,Barrett LE

更新日期：2019-12-06 00:00:00

abstract：:Previously, we identified ISRIB as a potent inhibitor of the integrated stress response (ISR) and showed that ISRIB makes cells resistant to the effects of eIF2α phosphorylation and enhances long-term memory in rodents (Sidrauski et al., 2013). Here, we show by genome-wide in vivo ribosome profiling that translation o...

journal_title：eLife

authors： Sidrauski C,McGeachy AM,Ingolia NT,Walter P

更新日期：2015-02-26 00:00:00

abstract：:Our understanding of nonlinear stimulus transformations by neural circuits is hindered by the lack of comprehensive yet interpretable computational modeling frameworks. Here, we propose a data-driven approach based on deep neural networks to directly model arbitrarily nonlinear stimulus-response mappings. Reformulatin...

journal_title：eLife

authors： Keshishian M,Akbari H,Khalighinejad B,Herrero JL,Mehta AD,Mesgarani N

更新日期：2020-06-26 00:00:00

abstract：:Although several sleep-regulating neuronal populations have been identified, little is known about how they interact with each other to control sleep/wake states. We previously identified neuropeptide VF (NPVF) and the hypothalamic neurons that produce it as a sleep-promoting system (Lee et al., 2017). Here we show us...

journal_title：eLife

authors： Lee DA,Oikonomou G,Cammidge T,Andreev A,Hong Y,Hurley H,Prober DA

更新日期：2020-12-18 00:00:00

abstract：:Cells respond to changes in their environment through signaling networks that modulate cytoskeleton and membrane organization to coordinate cell-cycle progression, polarized cell growth and multicellular development. Here, we define a novel regulatory mechanism by which the motor activity and function of the fission y...

journal_title：eLife

authors： Baker K,Gyamfi IA,Mashanov GI,Molloy JE,Geeves MA,Mulvihill DP

更新日期：2019-09-30 00:00:00

abstract：:Using a clever combination of genetic and biochemical tools researchers have shown that a transcription factor called USF1 has a central role in determining how mutations of the Clock gene manifest themselves in the behaviour of different mouse strains. ...

journal_title：eLife

authors： Demarque M,Schibler U

更新日期：2013-04-09 00:00:00

abstract：:A study of 3,500 children in the UK shows that data on socioeconomic background and previous educational achievements can better predict how students will perform at school than genetic data. ...

journal_title：eLife

authors： Janssens C

更新日期：2020-03-13 00:00:00

abstract：:Dendrites shape information flow in neurons. Yet, there is little consensus on the level of spatial complexity at which they operate. Through carefully chosen parameter fits, solvable in the least-squares sense, we obtain accurate reduced compartmental models at any level of complexity. We show that (back-propagating)...

journal_title：eLife

authors： Wybo WA,Jordan J,Ellenberger B,Marti Mengual U,Nevian T,Senn W

更新日期：2021-01-26 00:00:00

abstract：:Human SEMAPHORIN 5A (SEMA5A) is an autism susceptibility gene; however, its function in brain development is unknown. In this study, we show that mouse Sema5A negatively regulates synaptogenesis in early, developmentally born, hippocampal dentate granule cells (GCs). Sema5A is strongly expressed by GCs and regulates d...

journal_title：eLife

authors： Duan Y,Wang SH,Song J,Mironova Y,Ming GL,Kolodkin AL,Giger RJ

更新日期：2014-10-14 00:00:00

abstract：:The permeability barrier of nuclear pore complexes (NPCs) controls bulk nucleocytoplasmic exchange. It consists of nucleoporin domains rich in phenylalanine-glycine motifs (FG domains). As a bottom-up nanoscale model for the permeability barrier, we have used planar films produced with three different end-grafted FG d...

journal_title：eLife

authors： Zahn R,Osmanović D,Ehret S,Araya Callis C,Frey S,Stewart M,You C,Görlich D,Hoogenboom BW,Richter RP

更新日期：2016-04-08 00:00:00