Fly model sheds light on brain disease.

abstract：:Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understood. Using biochemical and single-molecule Förster resonance energy transfer (smFRET) exp...

journal_title：eLife

authors： Bao C,Loerch S,Ling C,Korostelev AA,Grigorieff N,Ermolenko DN

更新日期：2020-05-19 00:00:00

abstract：:Ki-67 and RepoMan have key roles during mitotic exit. Previously, we showed that Ki-67 organizes the mitotic chromosome periphery and recruits protein phosphatase 1 (PP1) to chromatin at anaphase onset, in a similar manner as RepoMan (Booth et al., 2014). Here we show how Ki-67 and RepoMan form mitotic exit phosphatas...

journal_title：eLife

authors： Kumar GS,Gokhan E,De Munter S,Bollen M,Vagnarelli P,Peti W,Page R

更新日期：2016-08-30 00:00:00

abstract：:Presence of multiple copies of the microtubule-binding NDC80 complex is an evolutionary conserved feature of kinetochores, points of attachment of chromosomes to spindle microtubules. This may enable multivalent attachments to microtubules, with implications that remain unexplored. Using recombinant human kinetochore ...

journal_title：eLife

authors： Volkov VA,Huis In 't Veld PJ,Dogterom M,Musacchio A

更新日期：2018-04-09 00:00:00

abstract：:Gene copy number alterations, tumor cell stemness, and the development of platinum chemotherapy resistance contribute to high-grade serous ovarian cancer (HGSOC) recurrence. Stem phenotypes involving Wnt-β-catenin, aldehyde dehydrogenase activities, intrinsic platinum resistance, and tumorsphere formation are here ass...

journal_title：eLife

authors： Diaz Osterman CJ,Ozmadenci D,Kleinschmidt EG,Taylor KN,Barrie AM,Jiang S,Bean LM,Sulzmaier FJ,Jean C,Tancioni I,Anderson K,Uryu S,Cordasco EA,Li J,Chen XL,Fu G,Ojalill M,Rappu P,Heino J,Mark AM,Xu G,Fisch KM,K

更新日期：2019-09-03 00:00:00

abstract：:The emergence of subpopulations that perform distinct metabolic roles has been observed in populations of genetically identical bacteria. ...

journal_title：eLife

authors： Dal Co A,Brannon C,Ackermann M

更新日期：2018-06-29 00:00:00

abstract：:Establishing a link between the nonsense-mediated decay pathway and a gene associated with programmed cell death could explain why this pathway is essential in most, but not all, eukaryotes. ...

journal_title：eLife

authors： Saveanu C,Jacquier A

更新日期：2016-04-27 00:00:00

abstract：:Single-molecule imaging experiments have shed new light on the methods used by the enzyme RecA to align single- and double-stranded DNA so that double-strand breaks can be repaired. ...

journal_title：eLife

authors： Gibb B,Greene EC

更新日期：2012-12-13 00:00:00

abstract：:Pattern completion, or the ability to retrieve stable neural activity patterns from noisy or partial cues, is a fundamental feature of memory. Theoretical studies indicate that recurrently connected auto-associative or discrete attractor networks can perform this process. Although pattern completion and attractor dyna...

journal_title：eLife

authors： Bolding KA,Nagappan S,Han BX,Wang F,Franks KM

更新日期：2020-07-14 00:00:00

abstract：:To initiate DNA replication, cells first load an MCM helicase double hexamer at origins in a reaction requiring ORC, Cdc6, and Cdt1, also called pre-replicative complex (pre-RC) assembly. The essential mechanistic role of Cdc6 ATP hydrolysis in this reaction is still incompletely understood. Here, we show that althoug...

journal_title：eLife

authors： Chang F,Riera A,Evrin C,Sun J,Li H,Speck C,Weinreich M

更新日期：2015-08-25 00:00:00

abstract：:Temporary circuits amplify spontaneous activity in the visual system of neonatal rats. ...

journal_title：eLife

authors： Fogerson PM,Huguenard JR

更新日期：2016-10-11 00:00:00

abstract：:Mitochondrial division, essential for survival in mammals, is enhanced by an inter-organellar process involving ER tubules encircling and constricting mitochondria. The force for constriction is thought to involve actin polymerization by the ER-anchored isoform of the formin protein inverted formin 2 (INF2). Unknown i...

journal_title：eLife

authors： Manor U,Bartholomew S,Golani G,Christenson E,Kozlov M,Higgs H,Spudich J,Lippincott-Schwartz J

更新日期：2015-08-25 00:00:00

abstract：:The Fluc family of F(-) ion channels protects prokaryotes and lower eukaryotes from the toxicity of environmental F(-). In bacteria, these channels are built as dual-topology dimers whereby the two subunits assemble in antiparallel transmembrane orientation. Recent crystal structures suggested that Fluc channels conta...

journal_title：eLife

authors： Last NB,Kolmakova-Partensky L,Shane T,Miller C

更新日期：2016-07-23 00:00:00

abstract：:Polypyrimidine-tract binding protein PTBP1 can repress splicing during the exon definition phase of spliceosome assembly, but the assembly steps leading to an exon definition complex (EDC) and how PTBP1 might modulate them are not clear. We found that PTBP1 binding in the flanking introns allowed normal U2AF and U1 sn...

journal_title：eLife

authors： Wongpalee SP,Vashisht A,Sharma S,Chui D,Wohlschlegel JA,Black DL

更新日期：2016-11-24 00:00:00

abstract：:MyD88 is the main adaptor molecule for TLR and IL-1R family members. Here, we demonstrated that T-cell intrinsic MyD88 signaling is required for proliferation, protection from apoptosis and expression of activation/memory genes during infection with the intracellular parasite Trypanosoma cruzi, as evidenced by transcr...

journal_title：eLife

authors： Oliveira AC,Gomes-Neto JF,Barbosa CD,Granato A,Reis BS,Santos BM,Fucs R,Canto FB,Nakaya HI,Nóbrega A,Bellio M

更新日期：2017-09-12 00:00:00

abstract：:Recent findings indicate a high level of specialization at the level of microcircuits and cell populations within brain structures with regards to the control of fear and anxiety. The hippocampus, however, has been treated as a unitary structure in anxiety and fear research despite mounting evidence that different hip...

journal_title：eLife

authors： Engin E,Smith KS,Gao Y,Nagy D,Foster RA,Tsvetkov E,Keist R,Crestani F,Fritschy JM,Bolshakov VY,Hajos M,Heldt SA,Rudolph U

更新日期：2016-03-14 00:00:00

abstract：:Developing neurons of the peripheral nervous system reach their targets via cues that support directional growth, a process known as axon guidance. In investigating how sympathetic axons reach the heart in mice, we discovered that a combination of guidance cues are employed in sequence to refine axon outgrowth, a proc...

journal_title：eLife

authors： Poltavski DM,Colombier P,Hu J,Duron A,Black BL,Makita T

更新日期：2019-02-08 00:00:00

abstract：:We present in vivo single-cell FRET measurements in the Escherichia coli chemotaxis system that reveal pervasive signaling variability, both across cells in isogenic populations and within individual cells over time. We quantify cell-to-cell variability of adaptation, ligand response, as well as steady-state output le...

journal_title：eLife

authors： Keegstra JM,Kamino K,Anquez F,Lazova MD,Emonet T,Shimizu TS

更新日期：2017-12-12 00:00:00

abstract：:Antibiotics are widely used in the treatment of bacterial infections. Although known for their microbicidal activity, antibiotics may also interfere with the host's immune system. Here, we analyzed the effects of bedaquiline (BDQ), an inhibitor of the mycobacterial ATP synthase, on human macrophages. Genome-wide gene ...

journal_title：eLife

authors： Giraud-Gatineau A,Coya JM,Maure A,Biton A,Thomson M,Bernard EM,Marrec J,Gutierrez MG,Larrouy-Maumus G,Brosch R,Gicquel B,Tailleux L

更新日期：2020-05-04 00:00:00

abstract：:Voltage-gated sodium channels are targets for a range of pharmaceutical drugs developed for the treatment of neurological diseases. Cannabidiol (CBD), the non-psychoactive compound isolated from cannabis plants, was recently approved for treatment of two types of epilepsy associated with sodium channel mutations. This...

journal_title：eLife

authors： Sait LG,Sula A,Ghovanloo MR,Hollingworth D,Ruben PC,Wallace BA

更新日期：2020-10-22 00:00:00

abstract：:AKT is implicated in neurological disorders. AKT has three isoforms, AKT1/AKT2/AKT3, with brain cell type-specific expression that may differentially influence behavior. Therefore, we examined single Akt isoform, conditional brain-specific Akt1, and double Akt1/3 mutant mice in behaviors relevant to neuropsychiatric d...

journal_title：eLife

authors： Wong H,Levenga J,LaPlante L,Keller B,Cooper-Sansone A,Borski C,Milstead R,Ehringer M,Hoeffer C

更新日期：2020-12-16 00:00:00

abstract：:Daily rhythms of food anticipatory activity (FAA) are regulated independently of the suprachiasmatic nucleus, which mediates entrainment of rhythms to light, but the neural circuits that establish FAA remain elusive. In this study, we show that mice lacking the dopamine D1 receptor (D1R KO mice) manifest greatly reduc...

journal_title：eLife

authors： Gallardo CM,Darvas M,Oviatt M,Chang CH,Michalik M,Huddy TF,Meyer EE,Shuster SA,Aguayo A,Hill EM,Kiani K,Ikpeazu J,Martinez JS,Purpura M,Smit AN,Patton DF,Mistlberger RE,Palmiter RD,Steele AD

更新日期：2014-09-12 00:00:00

abstract：:Spatial and temporal cues are required to specify neuronal diversity, but how these cues are integrated in neural progenitors remains unknown. Drosophila progenitors (neuroblasts) are a good model: they are individually identifiable with relevant spatial and temporal transcription factors known. Here we test whether s...

journal_title：eLife

authors： Sen SQ,Chanchani S,Southall TD,Doe CQ

更新日期：2019-01-29 00:00:00

abstract：:Being exposed to cannabinoids in the womb has different consequences for male and female rats. ...

journal_title：eLife

authors： Musardo S,Bellone C

更新日期：2018-09-28 00:00:00

abstract：:Armor plate changes in sticklebacks are a classic example of repeated adaptive evolution. Previous studies identified ectodysplasin (EDA) gene as the major locus controlling recurrent plate loss in freshwater fish, though the causative DNA alterations were not known. Here we show that freshwater EDA alleles have cis-a...

journal_title：eLife

authors： O'Brown NM,Summers BR,Jones FC,Brady SD,Kingsley DM

更新日期：2015-01-28 00:00:00

abstract：:D2 autoreceptors regulate dopamine release throughout the brain. Two isoforms of the D2 receptor, D2S and D2L, are expressed in midbrain dopamine neurons. Differential roles of these isoforms as autoreceptors are poorly understood. By virally expressing the isoforms in dopamine neurons of D2 receptor knockout mice, th...

journal_title：eLife

authors： Gantz SC,Robinson BG,Buck DC,Bunzow JR,Neve RL,Williams JT,Neve KA

更新日期：2015-08-26 00:00:00

abstract：:Kinesin-1 and cytoplasmic dynein are microtubule (MT) motors that transport intracellular cargoes. It remains unclear how these motors move along MTs densely coated with obstacles of various sizes in the cytoplasm. Here, we tested the ability of single and multiple motors to bypass synthetic obstacles on MTs in vitro....

journal_title：eLife

authors： Ferro LS,Can S,Turner MA,ElShenawy MM,Yildiz A

更新日期：2019-09-09 00:00:00

abstract：:The speed at which a cell fate decision in nematode worms evolves is due to the number of genes that control the decision, rather than to a high mutation rate. ...

journal_title：eLife

authors： Yang B,Rifkin SA

更新日期：2020-10-14 00:00:00

abstract：:Positive-strand RNA viruses, the largest genetic class of viruses, include numerous important pathogens such as Zika virus. These viruses replicate their RNA genomes in novel, membrane-bounded mini-organelles, but the organization of viral proteins and RNAs in these compartments has been largely unknown. We used cryo-...

journal_title：eLife

authors： Ertel KJ,Benefield D,Castaño-Diez D,Pennington JG,Horswill M,den Boon JA,Otegui MS,Ahlquist P

更新日期：2017-06-27 00:00:00

abstract：:During mitosis chromosomes reorganise into highly compact, rod-shaped forms, thought to consist of consecutive chromatin loops around a central protein scaffold. Condensin complexes are involved in chromatin compaction, but the contribution of other chromatin proteins, DNA sequence and histone modifications is less un...

journal_title：eLife

authors： Fitz-James MH,Tong P,Pidoux AL,Ozadam H,Yang L,White SA,Dekker J,Allshire RC

更新日期：2020-09-11 00:00:00

abstract：:Genome-wide association analyses have uncovered multiple genomic regions associated with T2D, but identification of the causal variants at these remains a challenge. There is growing interest in the potential of deep learning models - which predict epigenome features from DNA sequence - to support inference concerning...

journal_title：eLife

authors： Wesolowska-Andersen A,Zhuo Yu G,Nylander V,Abaitua F,Thurner M,Torres JM,Mahajan A,Gloyn AL,McCarthy MI

更新日期：2020-01-27 00:00:00