Isoform-specific roles for AKT in affective behavior, spatial memory, and extinction related to psychiatric disorders.

abstract：:As the staff of the MRC Laboratory of Molecular Biology settle into their new building in Cambridge, its director Hugh Pelham explains the challenges of living up to its prestigious past. ...

journal_title：eLife

authors： Pelham H

更新日期：2013-05-28 00:00:00

abstract：:When a T cell and an antigen-presenting cell form an immunological synapse, rapid dynein-driven translocation of the centrosome toward the contact site leads to reorganization of microtubules and associated organelles. Currently, little is known about how the regulation of microtubule dynamics contributes to this proc...

journal_title：eLife

authors： Hooikaas PJ,Damstra HG,Gros OJ,van Riel WE,Martin M,Smits YT,van Loosdregt J,Kapitein LC,Berger F,Akhmanova A

更新日期：2020-12-21 00:00:00

abstract：:Myelin destruction is followed by resident glia activation and mobilization of endogenous progenitors (OPC) which participate in myelin repair. Here we show that in response to demyelination, mature oligodendrocytes (OLG) bordering the lesion express Ndst1, a key enzyme for heparan sulfates (HS) synthesis. Ndst1+ OLG ...

journal_title：eLife

authors： Macchi M,Magalon K,Zimmer C,Peeva E,El Waly B,Brousse B,Jaekel S,Grobe K,Kiefer F,Williams A,Cayre M,Durbec P

更新日期：2020-06-09 00:00:00

abstract：:Different coding strategies are used to represent odor information at various stages of the mammalian olfactory system. A temporal latency code represents odor identity in olfactory bulb (OB), but this temporal information is discarded in piriform cortex (PCx) where odor identity is instead encoded through ensemble me...

journal_title：eLife

authors： Stern M,Bolding KA,Abbott LF,Franks KM

更新日期：2018-03-29 00:00:00

abstract：:The time it takes a sound to travel from source to ear differs between the ears and creates an interaural delay. It varies systematically with spatial direction and is generally modeled as a pure time delay, independent of frequency. In acoustical recordings, we found that interaural delay varies with frequency at a f...

journal_title：eLife

authors： Benichoux V,Fontaine B,Franken TP,Karino S,Joris PX,Brette R

更新日期：2015-04-27 00:00:00

abstract：:The characteristics of goal-directed actions tend to resemble those of previously executed actions, but it is unclear whether such effects depend strictly on action history, or also reflect context-dependent processes related to predictive motor planning. Here we manipulated the time available to initiate movements af...

journal_title：eLife

authors： Marinovic W,Poh E,de Rugy A,Carroll TJ

更新日期：2017-10-23 00:00:00

abstract：:Engineered molecules based on human cytokines have potential uses in research and medicine. ...

journal_title：eLife

authors： Villarino A,O'Shea JJ

更新日期：2017-05-12 00:00:00

abstract：:The neural control of sugar consumption is critical for normal metabolism. In contrast to sugar-sensing taste neurons that promote consumption, we identify a taste neuron that limits sucrose consumption in Drosophila. Silencing of the neuron increases sucrose feeding; optogenetic activation decreases it. The feeding i...

journal_title：eLife

authors： Joseph RM,Sun JS,Tam E,Carlson JR

更新日期：2017-03-23 00:00:00

abstract：:The actomyosin ring generates force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organization and the mechanism of contractility is not well understood. We quantified actin filament order in human cells using fluorescence polarization microscopy and found that cleavage furrow ingression...

journal_title：eLife

authors： Spira F,Cuylen-Haering S,Mehta S,Samwer M,Reversat A,Verma A,Oldenbourg R,Sixt M,Gerlich DW

更新日期：2017-11-06 00:00:00

abstract：:Genetic analyses of 15 species of yeast have shed new light on the divergence of gene regulation during evolution, with significant changes occurring after an event in which a whole genome was duplicated. ...

journal_title：eLife

authors： Ragan MA

更新日期：2013-06-18 00:00:00

abstract：:Early mouse development is accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2), which is essential for embryonic development. Here we show that genome-wide accumulation of H3K9me2 is crucial for postimplantation development, and coincides with r...

journal_title：eLife

authors： Zylicz JJ,Dietmann S,Günesdogan U,Hackett JA,Cougot D,Lee C,Surani MA

更新日期：2015-11-09 00:00:00

abstract：:Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased in...

journal_title：eLife

authors： Wales P,Schuberth CE,Aufschnaiter R,Fels J,García-Aguilar I,Janning A,Dlugos CP,Schäfer-Herte M,Klingner C,Wälte M,Kuhlmann J,Menis E,Hockaday Kang L,Maier KC,Hou W,Russo A,Higgs HN,Pavenstädt H,Vogl T,Roth J,Qual

更新日期：2016-12-06 00:00:00

abstract：:Newly born cells either continue to proliferate or exit the cell division cycle. This decision involves delaying expression of Cyclin E that promotes DNA replication. ORC1, the Origin Recognition Complex (ORC) large subunit, is inherited into newly born cells after it binds to condensing chromosomes during the precedi...

journal_title：eLife

authors： Hossain M,Stillman B

更新日期：2016-07-26 00:00:00

abstract：:Bacterial flagella mediate host-microbe interactions through tissue tropism during colonization, as well as by activating immune responses. The flagellar shaft of some bacteria, including several human pathogens, is encased in a membranous sheath of unknown function. While it has been hypothesized that the sheath may ...

journal_title：eLife

authors： Brennan CA,Hunt JR,Kremer N,Krasity BC,Apicella MA,McFall-Ngai MJ,Ruby EG

更新日期：2014-03-04 00:00:00

abstract：:Recurrent somatic mutations of H3F3A in aggressive pediatric high-grade gliomas generate K27M or G34R/V mutant histone H3.3. H3.3-G34R/V mutants are common in tumors with mutations in p53 and ATRX, an H3.3-specific chromatin remodeler. To gain insight into the role of H3-G34R, we generated fission yeast that express o...

journal_title：eLife

authors： Yadav RK,Jablonowski CM,Fernandez AG,Lowe BR,Henry RA,Finkelstein D,Barnum KJ,Pidoux AL,Kuo YM,Huang J,O'Connell MJ,Andrews AJ,Onar-Thomas A,Allshire RC,Partridge JF

更新日期：2017-07-18 00:00:00

abstract：:In endoplasmic reticulum-associated protein degradation (ERAD), membrane proteins are ubiquitinated, extracted from the membrane, and degraded by the proteasome. The cytosolic ATPase Cdc48 drives extraction by pulling on polyubiquitinated substrates. How hydrophobic transmembrane (TM) segments are moved from the phosp...

journal_title：eLife

authors： Schmidt CC,Vasic V,Stein A

更新日期：2020-06-26 00:00:00

abstract：:Previously, we showed that serum and monocytes from patients with CF exhibit an enhanced NLRP3-inflammasome signature with increased IL-18, IL-1β, caspase-1 activity and ASC speck release (Scambler et al. eLife 2019). Here we show that CFTR modulators down regulate this exaggerated proinflammatory response following L...

journal_title：eLife

authors： Jarosz-Griffiths HH,Scambler T,Wong CH,Lara-Reyna S,Holbrook J,Martinon F,Savic S,Whitaker P,Etherington C,Spoletini G,Clifton I,Mehta A,McDermott MF,Peckham D

更新日期：2020-03-02 00:00:00

abstract：:We present in vivo single-cell FRET measurements in the Escherichia coli chemotaxis system that reveal pervasive signaling variability, both across cells in isogenic populations and within individual cells over time. We quantify cell-to-cell variability of adaptation, ligand response, as well as steady-state output le...

journal_title：eLife

authors： Keegstra JM,Kamino K,Anquez F,Lazova MD,Emonet T,Shimizu TS

更新日期：2017-12-12 00:00:00

abstract：:Smoothened (Smo) inhibition by Patched (Ptch) is central to Hedgehog (Hh) signaling. Ptch, a proton driven antiporter, is required for Smo inhibition via an unknown mechanism. Hh ligand binding to Ptch reverses this inhibition and activated Smo initiates the Hh response. To determine whether Ptch inhibits Smo strictly...

journal_title：eLife

authors： Roberts B,Casillas C,Alfaro AC,Jägers C,Roelink H

更新日期：2016-08-23 00:00:00

abstract：:Gene transcription can be activated by decreasing the duration of RNA polymerase II pausing in the promoter-proximal region, but how this is achieved remains unclear. Here we use a 'multi-omics' approach to demonstrate that the duration of polymerase pausing generally limits the productive frequency of transcription i...

journal_title：eLife

authors： Gressel S,Schwalb B,Decker TM,Qin W,Leonhardt H,Eick D,Cramer P

更新日期：2017-10-10 00:00:00

abstract：:Bacteria of the genus Shigella cause shigellosis, a severe gastrointestinal disease that is a major cause of diarrhea-associated mortality in humans. Mice are highly resistant to Shigella and the lack of a tractable physiological model of shigellosis has impeded our understanding of this important human disease. Here,...

journal_title：eLife

authors： Mitchell PS,Roncaioli JL,Turcotte EA,Goers L,Chavez RA,Lee AY,Lesser CF,Rauch I,Vance RE

更新日期：2020-10-19 00:00:00

abstract：:The gut microbiota of patients recently diagnosed with rheumatoid arthritis is enriched in microbes belonging to the Prevotella genus. ...

journal_title：eLife

authors： Mathis D

更新日期：2013-11-05 00:00:00

abstract：:The clone size distribution of the human naive T-cell receptor (TCR) repertoire is an important determinant of adaptive immunity. We estimated the abundance of TCR sequences in samples of naive T cells from blood using an accurate quantitative sequencing protocol. We observe most TCR sequences only once, consistent wi...

journal_title：eLife

authors： de Greef PC,Oakes T,Gerritsen B,Ismail M,Heather JM,Hermsen R,Chain B,de Boer RJ

更新日期：2020-03-18 00:00:00

abstract：:Huntington disease (HD) is a neurological disorder caused by polyglutamine expansions in mutated Huntingtin (mHtt) proteins, rendering them prone to form inclusion bodies (IB). We report that in yeast, such IB formation is a factor-dependent process subjected to age-related decline. A genome-wide, high-content imaging...

journal_title：eLife

authors： Yang J,Hao X,Cao X,Liu B,Nyström T

更新日期：2016-04-01 00:00:00

abstract：:We recently reported that the C2AB portion of Synaptotagmin 1 (Syt1) could self-assemble into Ca(2+)-sensitive ring-like oligomers on membranes, which could potentially regulate neurotransmitter release. Here we report that analogous ring-like oligomers assemble from the C2AB domains of other Syt isoforms (Syt2, Syt7,...

journal_title：eLife

authors： Zanetti MN,Bello OD,Wang J,Coleman J,Cai Y,Sindelar CV,Rothman JE,Krishnakumar SS

更新日期：2016-07-19 00:00:00

abstract：:Two-dimensional (2D) human skeletal muscle fiber cultures are ill-equipped to support the contractile properties of maturing muscle fibers. This limits their application to the study of adult human neuromuscular junction (NMJ) development, a process requiring maturation of muscle fibers in the presence of motor neuron...

journal_title：eLife

authors： Afshar Bakooshli M,Lippmann ES,Mulcahy B,Iyer N,Nguyen CT,Tung K,Stewart BA,van den Dorpel H,Fuehrmann T,Shoichet M,Bigot A,Pegoraro E,Ahn H,Ginsberg H,Zhen M,Ashton RS,Gilbert PM

更新日期：2019-05-14 00:00:00

abstract：:Mitochondrial division, essential for survival in mammals, is enhanced by an inter-organellar process involving ER tubules encircling and constricting mitochondria. The force for constriction is thought to involve actin polymerization by the ER-anchored isoform of the formin protein inverted formin 2 (INF2). Unknown i...

journal_title：eLife

authors： Manor U,Bartholomew S,Golani G,Christenson E,Kozlov M,Higgs H,Spudich J,Lippincott-Schwartz J

更新日期：2015-08-25 00:00:00

abstract：:Protein aggregation appears to originate from partially unfolded conformations that are sampled through stochastic fluctuations of the native protein. It has been a challenge to characterize these fluctuations, under native like conditions. Here, the conformational dynamics of the full-length (23-231) mouse prion prot...

journal_title：eLife

authors： Goluguri RR,Sen S,Udgaonkar J

更新日期：2019-04-26 00:00:00

abstract：:Jellyfish, with their tetraradial symmetry, offer a novel paradigm for addressing patterning mechanisms during regeneration. Here we show that an interplay between mechanical forces, cell migration and proliferation allows jellyfish fragments to regain shape and functionality rapidly, notably by efficient restoration ...

journal_title：eLife

authors： Sinigaglia C,Peron S,Eichelbrenner J,Chevalier S,Steger J,Barreau C,Houliston E,Leclère L

更新日期：2020-09-07 00:00:00

abstract：:The brain has an impressive ability to withstand neural damage. Diseases that kill neurons can go unnoticed for years, and incomplete brain lesions or silencing of neurons often fail to produce any behavioral effect. How does the brain compensate for such damage, and what are the limits of this compensation? We propos...

journal_title：eLife

authors： Barrett DG,Denève S,Machens CK

更新日期：2016-12-09 00:00:00