Kinesin and dynein use distinct mechanisms to bypass obstacles.

abstract：:Msp1 is a conserved AAA ATPase in budding yeast localized to mitochondria where it prevents accumulation of mistargeted tail-anchored (TA) proteins, including the peroxisomal TA protein Pex15. Msp1 also resides on peroxisomes but it remains unknown how native TA proteins on mitochondria and peroxisomes evade Msp1 surv...

journal_title：eLife

authors： Weir NR,Kamber RA,Martenson JS,Denic V

更新日期：2017-09-14 00:00:00

abstract：:Cell-type-specific 3D organization of the genome is unrecognizable during mitosis. It remains unclear how essential positional information is transmitted through cell division such that a daughter cell recapitulates the spatial genome organization of the parent. Lamina-associated domains (LADs) are regions of repressi...

journal_title：eLife

authors： Poleshko A,Smith CL,Nguyen SC,Sivaramakrishnan P,Wong KG,Murray JI,Lakadamyali M,Joyce EF,Jain R,Epstein JA

更新日期：2019-10-01 00:00:00

abstract：:Seed dormancy is a complex life history trait that determines the timing of germination and is crucial for local adaptation. Genetic studies of dormancy are challenging, because the trait is highly plastic and strongly influenced by the maternal environment. Using a combination of statistical and experimental approach...

journal_title：eLife

authors： Kerdaffrec E,Filiault DL,Korte A,Sasaki E,Nizhynska V,Seren Ü,Nordborg M

更新日期：2016-12-14 00:00:00

abstract：:Grid cells in the brain respond when an animal occupies a periodic lattice of 'grid fields' during navigation. Grids are organized in modules with different periodicity. We propose that the grid system implements a hierarchical code for space that economizes the number of neurons required to encode location with a giv...

journal_title：eLife

authors： Wei XX,Prentice J,Balasubramanian V

更新日期：2015-09-03 00:00:00

abstract：:CRISPR-based homing gene drives have sparked both enthusiasm and deep concerns due to their potential for genetically altering entire species. This raises the question about our ability to prevent the unintended spread of such drives from the laboratory into a natural population. Here, we experimentally demonstrate th...

journal_title：eLife

authors： Champer J,Chung J,Lee YL,Liu C,Yang E,Wen Z,Clark AG,Messer PW

更新日期：2019-01-22 00:00:00

abstract：:We aimed to study time trends and levels of mean total cholesterol and lipid fractions, and dyslipidaemias prevalence in Latin America and the Caribbean (LAC). Systematic-review and meta-analysis of population-based studies in which lipid (total cholesterol [TC; 86 studies; 168,553 people], HDL-Cholesterol [HDL-C; 84 ...

journal_title：eLife

authors： Carrillo-Larco RM,Benites-Moya CJ,Anza-Ramirez C,Albitres-Flores L,Sánchez-Velazco D,Pacheco-Barrios N,Bernabe-Ortiz A

更新日期：2020-08-18 00:00:00

abstract：:To initiate DNA replication, cells first load an MCM helicase double hexamer at origins in a reaction requiring ORC, Cdc6, and Cdt1, also called pre-replicative complex (pre-RC) assembly. The essential mechanistic role of Cdc6 ATP hydrolysis in this reaction is still incompletely understood. Here, we show that althoug...

journal_title：eLife

authors： Chang F,Riera A,Evrin C,Sun J,Li H,Speck C,Weinreich M

更新日期：2015-08-25 00:00:00

abstract：:During sleep, the brain experiences large fluctuations in blood volume and altered coupling between neural and vascular signals. ...

journal_title：eLife

authors： Williams SD,Lewis LD

更新日期：2020-12-23 00:00:00

abstract：:SUMO-modification of nuclear proteins has profound effects on gene expression. However, non-toxic chemical tools that modulate sumoylation in cells are lacking. Here, to identify small molecule sumoylation inhibitors we developed a cell-based screen that focused on the well-sumoylated substrate, human Liver Receptor H...

journal_title：eLife

authors： Suzawa M,Miranda DA,Ramos KA,Ang KK,Faivre EJ,Wilson CG,Caboni L,Arkin MR,Kim YS,Fletterick RJ,Diaz A,Schneekloth JS,Ingraham HA

更新日期：2015-12-11 00:00:00

abstract：:Physiological adaptation to proteotoxic stress in the endoplasmic reticulum (ER) requires retrotranslocation of misfolded proteins into the cytoplasm for ubiquitination and elimination by ER-associated degradation (ERAD). A surprising paradox emerging from recent studies is that ubiquitin ligases (E3s) and deubiquitin...

journal_title：eLife

authors： Liu Y,Soetandyo N,Lee JG,Liu L,Xu Y,Clemons WM Jr,Ye Y

更新日期：2014-01-01 00:00:00

abstract：:The integrity of the nuclear membranes coupled to the selective barrier of nuclear pore complexes (NPCs) are essential for the segregation of nucleoplasm and cytoplasm. Mechanical membrane disruption or perturbation to NPC assembly triggers an ESCRT-dependent surveillance system that seals nuclear pores: how these por...

journal_title：eLife

authors： Thaller DJ,Allegretti M,Borah S,Ronchi P,Beck M,Lusk CP

更新日期：2019-04-03 00:00:00

abstract：:Antiviral development is plagued by drug resistance and genetic barriers to resistance are needed. For HIV and hepatitis C virus (HCV), combination therapy has proved life-saving. The targets of direct-acting antivirals for HCV infection are NS3/4A protease, NS5A phosphoprotein and NS5B polymerase. Differential visual...

journal_title：eLife

authors： van Buuren N,Tellinghuisen TL,Richardson CD,Kirkegaard K

更新日期：2018-03-28 00:00:00

abstract：:Vesicle fusion is mediated by an assembly of SNARE proteins between opposing membranes, but it is unknown whether transmembrane domains (TMDs) of SNARE proteins serve mechanistic functions that go beyond passive anchoring of the force-generating SNAREpin to the fusing membranes. Here, we show that conformational flexi...

journal_title：eLife

authors： Dhara M,Yarzagaray A,Makke M,Schindeldecker B,Schwarz Y,Shaaban A,Sharma S,Böckmann RA,Lindau M,Mohrmann R,Bruns D

更新日期：2016-06-25 00:00:00

abstract：:Cells respond to changes in their environment through signaling networks that modulate cytoskeleton and membrane organization to coordinate cell-cycle progression, polarized cell growth and multicellular development. Here, we define a novel regulatory mechanism by which the motor activity and function of the fission y...

journal_title：eLife

authors： Baker K,Gyamfi IA,Mashanov GI,Molloy JE,Geeves MA,Mulvihill DP

更新日期：2019-09-30 00:00:00

abstract：:Cortical plasticity is fundamental to motor recovery following cortical perturbation. However, it is still unclear how this plasticity is induced at a functional circuit level. Here, we investigated motor recovery and underlying neural plasticity upon optogenetic suppression of a cortical area for eye movement. Using ...

journal_title：eLife

authors： Sato TR,Itokazu T,Osaki H,Ohtake M,Yamamoto T,Sohya K,Maki T,Sato TK

更新日期：2019-11-05 00:00:00

abstract：:Determining the etiologic basis of the mutations that are responsible for cancer is one of the fundamental challenges in modern cancer research. Different mutational processes induce different types of DNA mutations, providing 'mutational signatures' that have led to key insights into cancer etiology. The most widely ...

journal_title：eLife

authors： Afsari B,Kuo A,Zhang Y,Li L,Lahouel K,Danilova L,Favorov A,Rosenquist TA,Grollman AP,Kinzler KW,Cope L,Vogelstein B,Tomasetti C

更新日期：2021-01-25 00:00:00

abstract：:Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease. Although rigorous efforts identified the presence of 'cancer stem cells (CSCs)' in PDAC and molecular markers for them, stem cell dynamics in vivo have not been clearly demonstrated. Here we focused on Doublecortin-like kinase 1 (Dclk1), known as a CSC ...

journal_title：eLife

authors： Maruno T,Fukuda A,Goto N,Tsuda M,Ikuta K,Hiramatsu Y,Ogawa S,Nakanishi Y,Yamaga Y,Yoshioka T,Takaori K,Uemoto S,Saur D,Chiba T,Seno H

更新日期：2021-01-04 00:00:00

abstract：:IRE1α is an endoplasmic reticulum (ER) localized endonuclease activated by misfolded proteins in the ER. Previously, we demonstrated that IRE1α forms a complex with the Sec61 translocon, to which its substrate XBP1u mRNA is recruited for cleavage during ER stress (Plumb et al., 2015). Here, we probe IRE1α complexes in...

journal_title：eLife

authors： Sundaram A,Plumb R,Appathurai S,Mariappan M

更新日期：2017-05-15 00:00:00

abstract：:Most funding agencies rely on peer review to evaluate grant applications and proposals, but research into the use of this process by funding agencies has been limited. Here we explore if two changes to the organization of peer review for proposals submitted to various funding actions by the European Union has an influ...

journal_title：eLife

authors： Pina DG,Buljan I,Hren D,Marušić A

更新日期：2021-01-13 00:00:00

abstract：:Current models successfully describe the auditory cortical response to natural sounds with a set of spectro-temporal features. However, these models have hardly been linked to the ill-understood neurobiological changes that occur in the aging auditory cortex. Modelling the hemodynamic response to a rich natural sound ...

journal_title：eLife

authors： Erb J,Schmitt LM,Obleser J

更新日期：2020-07-03 00:00:00

abstract：:T follicular helper cells (Tfh) are critical for the longevity and quality of antibody-mediated protection against infection. Yet few signaling pathways have been identified to be unique solely to Tfh development. ROQUIN is a post-transcriptional repressor of T cells, acting through its ROQ domain to destabilize mRNA ...

journal_title：eLife

authors： Ramiscal RR,Parish IA,Lee-Young RS,Babon JJ,Blagih J,Pratama A,Martin J,Hawley N,Cappello JY,Nieto PF,Ellyard JI,Kershaw NJ,Sweet RA,Goodnow CC,Jones RG,Febbraio MA,Vinuesa CG,Athanasopoulos V

更新日期：2015-10-23 00:00:00

abstract：:Most cellular features have a range of states, but understanding the mechanisms responsible for interspecific divergence is a challenge for evolutionary cell biology. Models are developed for the distribution of mean phenotypes likely to evolve under the joint forces of mutation and genetic drift in the face of consta...

journal_title：eLife

authors： Lynch M

更新日期：2018-06-21 00:00:00

abstract：:Evolutionary changes in transcription networks are an important source of diversity across species, yet the quantitative consequences of network evolution have rarely been studied. Here we consider the transcriptional 'rewiring' of the three GAL genes that encode the enzymes needed for cells to convert galactose to gl...

journal_title：eLife

authors： Dalal CK,Zuleta IA,Mitchell KF,Andes DR,El-Samad H,Johnson AD

更新日期：2016-09-10 00:00:00

abstract：:The asymmetric outer membrane (OM) of Gram-negative bacteria functions as a selective permeability barrier to the environment. Perturbations to OM lipid asymmetry sensitize the cell to antibiotics. As such, mechanisms involved in lipid asymmetry are fundamental to our understanding of OM lipid homeostasis. One such me...

journal_title：eLife

authors： Powers MJ,Simpson BW,Trent MS

更新日期：2020-09-03 00:00:00

abstract：:Rapid diagnostic tests (RDTs) have transformed malaria diagnosis. The most prevalent P. falciparum RDTs detect histidine-rich protein 2 (PfHRP2). However, pfhrp2 gene deletions yielding false-negative RDTs, first reported in South America in 2010, have been confirmed in Africa and Asia. We developed a mathematical mod...

journal_title：eLife

authors： Watson OJ,Slater HC,Verity R,Parr JB,Mwandagalirwa MK,Tshefu A,Meshnick SR,Ghani AC

更新日期：2017-08-24 00:00:00

abstract：:Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature of this pro-inflammatory response in mammals is the complex remodeling of cellular metabolism towards aerobic glycolysis. Although the function of bactericidal macrophages is highly con...

journal_title：eLife

authors： Krejčová G,Danielová A,Nedbalová P,Kazek M,Strych L,Chawla G,Tennessen JM,Lieskovská J,Jindra M,Doležal T,Bajgar A

更新日期：2019-10-14 00:00:00

abstract：:We recently reported that the C2AB portion of Synaptotagmin 1 (Syt1) could self-assemble into Ca(2+)-sensitive ring-like oligomers on membranes, which could potentially regulate neurotransmitter release. Here we report that analogous ring-like oligomers assemble from the C2AB domains of other Syt isoforms (Syt2, Syt7,...

journal_title：eLife

authors： Zanetti MN,Bello OD,Wang J,Coleman J,Cai Y,Sindelar CV,Rothman JE,Krishnakumar SS

更新日期：2016-07-19 00:00:00

abstract：:We previously showed in Drosophila melanogaster embryos that low-affinity Ultrabithorax (Ubx)-responsive shavenbaby (svb) enhancers drive expression using localized transcriptional environments and that active svb enhancers on different chromosomes tended to colocalize (Tsai et al., 2017). Here, we test the hypothesis...

journal_title：eLife

authors： Tsai A,Alves MR,Crocker J

更新日期：2019-07-11 00:00:00

abstract：:Antagonistic receptive field surrounds are a near-universal property of early sensory processing. A key assumption in many models for retinal ganglion cell encoding is that receptive field surrounds are added only to the fully formed center signal. But anatomical and functional observations indicate that surrounds are...

journal_title：eLife

authors： Turner MH,Schwartz GW,Rieke F

更新日期：2018-09-06 00:00:00

abstract：:Delineating the basic cellular components of cortical inhibitory circuits remains a fundamental issue in order to understand their specific contributions to microcircuit function. It is still unclear how current classifications of cortical interneuron subtypes relate to biological processes such as their developmental...

journal_title：eLife

authors： Niquille M,Limoni G,Markopoulos F,Cadilhac C,Prados J,Holtmaat A,Dayer A

更新日期：2018-03-20 00:00:00