Data-driven reduction of dendritic morphologies with preserved dendro-somatic responses.

abstract：:Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease involving cytotoxic conformations of Cu, Zn superoxide dismutase (SOD1). A major challenge in understanding ALS disease pathology has been the identification and atomic-level characterization of these conformers. Here, we use a combination ...

journal_title：eLife

authors： Sekhar A,Rumfeldt JA,Broom HR,Doyle CM,Bouvignies G,Meiering EM,Kay LE

更新日期：2015-06-23 00:00:00

abstract：:Bacterial cells utilize monitoring substrates, which undergo force-sensitive translation elongation arrest, to feedback-regulate a Sec-related gene. Vibrio alginolyticus VemP controls the expression of SecD/F that stimulates a late step of translocation by undergoing export-regulated elongation arrest. Here, we attemp...

journal_title：eLife

authors： Miyazaki R,Akiyama Y,Mori H

更新日期：2020-12-15 00:00:00

abstract：:Correct neuronal development requires tailored neurite outgrowth. Neurite outgrowth is driven in part by microtubule-sliding - the transport of microtubules along each other. We have recently demonstrated that a 'mitotic' kinesin-6 (Pavarotti in Drosophila) effectively inhibits microtubule-sliding and neurite outgrowt...

journal_title：eLife

authors： Norkett R,Del Castillo U,Lu W,Gelfand VI

更新日期：2020-02-05 00:00:00

abstract：:Development of specialized cells and structures in the heart is regulated by spatially -restricted molecular pathways. Disruptions in these pathways can cause severe congenital cardiac malformations or functional defects. To better understand these pathways and how they regulate cardiac development we used tomo-seq, c...

journal_title：eLife

authors： Burkhard SB,Bakkers J

更新日期：2018-02-05 00:00:00

abstract：:Optical imaging has become a powerful tool for studying brains in vivo. The opacity of adult brains makes microendoscopy, with an optical probe such as a gradient index (GRIN) lens embedded into brain tissue to provide optical relay, the method of choice for imaging neurons and neural activity in deeply buried brain s...

journal_title：eLife

authors： Meng G,Liang Y,Sarsfield S,Jiang WC,Lu R,Dudman JT,Aponte Y,Ji N

更新日期：2019-01-04 00:00:00

abstract：:V-ATPase is an energy converting enzyme, coupling ATP hydrolysis/synthesis in the hydrophilic V1 domain, with proton flow through the Vo membrane domain, via rotation of the central rotor complex relative to the surrounding stator apparatus. Upon dissociation from the V1 domain, the Vo domain of the eukaryotic V-ATPas...

journal_title：eLife

authors： Kishikawa JI,Nakanishi A,Furuta A,Kato T,Namba K,Tamakoshi M,Mitsuoka K,Yokoyama K

更新日期：2020-07-08 00:00:00

abstract：:The IFNλ family of interferons controls the spread of viruses in the upper respiratory tract and transmission between mice. ...

journal_title：eLife

authors： Wack A

更新日期：2018-05-24 00:00:00

abstract：:Proneural bHLH proteins are transcriptional regulators of neural fate specification. Extra macrochaetae (Emc) forms inactive heterodimers with both proneural bHLH proteins and their bHLH partners (represented in Drosophila by Daughterless). It is generally thought that varying levels of Emc define a prepattern that de...

journal_title：eLife

authors： Li K,Baker NE

更新日期：2018-04-24 00:00:00

abstract：:Calcineurin is responsible for mediating a wide variety of cellular processes in response to dynamic calcium (Ca(2+)) signals, yet the precise mechanisms involved in the spatiotemporal control of calcineurin signaling are poorly understood. Here, we use genetically encoded fluorescent biosensors to directly probe the ...

journal_title：eLife

authors： Mehta S,Aye-Han NN,Ganesan A,Oldach L,Gorshkov K,Zhang J

更新日期：2014-07-23 00:00:00

abstract：:Cardiac conduction defects decrease life expectancy in myotonic dystrophy type 1 (DM1), a CTG repeat disorder involving misbalance between two RNA binding factors, MBNL1 and CELF1. However, how DM1 condition translates into conduction disorders remains poorly understood. Here we simulated MBNL1 and CELF1 misbalance in...

journal_title：eLife

authors： Auxerre-Plantié E,Nakamori M,Renaud Y,Huguet A,Choquet C,Dondi C,Miquerol L,Takahashi MP,Gourdon G,Junion G,Jagla T,Zmojdzian M,Jagla K

更新日期：2019-12-12 00:00:00

abstract：:Heterogeneity broadly exists in various cell types both during development and at homeostasis. Investigating heterogeneity is crucial for comprehensively understanding the complexity of ontogeny, dynamics, and function of specific cell types. Traditional bulk-labeling techniques are incompetent to dissect heterogeneit...

journal_title：eLife

authors： He S,Tian Y,Feng S,Wu Y,Shen X,Chen K,He Y,Sun Q,Li X,Xu J,Wen Z,Qu JY

更新日期：2020-01-06 00:00:00

abstract：:The asymmetric outer membrane (OM) of Gram-negative bacteria functions as a selective permeability barrier to the environment. Perturbations to OM lipid asymmetry sensitize the cell to antibiotics. As such, mechanisms involved in lipid asymmetry are fundamental to our understanding of OM lipid homeostasis. One such me...

journal_title：eLife

authors： Powers MJ,Simpson BW,Trent MS

更新日期：2020-09-03 00:00:00

abstract：:Conventional Ly6Chi monocytes have developmental plasticity for a spectrum of differentiated phagocytes. Here we show, using conditional deletion strategies in a mouse model of Toll-like receptor (TLR) 7-induced inflammation, that the spectrum of developmental cell fates of Ly6Chi monocytes, and the resultant inflamma...

journal_title：eLife

authors： Gamrekelashvili J,Kapanadze T,Sablotny S,Ratiu C,Dastagir K,Lochner M,Karbach S,Wenzel P,Sitnow A,Fleig S,Sparwasser T,Kalinke U,Holzmann B,Haller H,Limbourg FP

更新日期：2020-07-29 00:00:00

abstract：:Negative regulators of adult neurogenesis are of particular interest as targets to enhance neuronal repair, but few have yet been identified. Planarians can regenerate their entire CNS using pluripotent adult stem cells, and this process is robustly regulated to ensure that new neurons are produced in proper abundance...

journal_title：eLife

authors： Karge A,Bonar NA,Wood S,Petersen CP

更新日期：2020-01-20 00:00:00

abstract：:The nematode Caenorhabditis elegans possesses a simple embryonic nervous system with few enough neurons that the growth of each cell could be followed to provide a systems-level view of development. However, studies of single cell development have largely been conducted in fixed or pre-twitching live embryos, because ...

journal_title：eLife

authors： Christensen RP,Bokinsky A,Santella A,Wu Y,Marquina-Solis J,Guo M,Kovacevic I,Kumar A,Winter PW,Tashakkori N,McCreedy E,Liu H,McAuliffe M,Mohler W,Colón-Ramos DA,Bao Z,Shroff H

更新日期：2015-12-03 00:00:00

abstract：:Massed synchronised neuronal firing is detrimental to information processing. When networks of task-irrelevant neurons fire in unison, they mask the signal generated by task-critical neurons. On a macroscopic level, such synchronisation can contribute to alpha/beta (8-30 Hz) oscillations. Reducing the amplitude of the...

journal_title：eLife

authors： Griffiths BJ,Mayhew SD,Mullinger KJ,Jorge J,Charest I,Wimber M,Hanslmayr S

更新日期：2019-11-29 00:00:00

abstract：:Faithful chromosome segregation in all eukaryotes relies on centromeres, the chromosomal sites that recruit kinetochore proteins and mediate spindle attachment during cell division. The centromeric histone H3 variant, CenH3, is the defining chromatin component of centromeres in most eukaryotes, including animals, fung...

journal_title：eLife

authors： Drinnenberg IA,deYoung D,Henikoff S,Malik HS

更新日期：2014-09-23 00:00:00

abstract：:Eukaryotic kinetochores connect spindlemicrotubules to chromosomal centromeres. A group of proteins called the Ctf19 complex (Ctf19c) in yeast and the constitutive centromere associated network (CCAN) in other organisms creates the foundation of a kinetochore. The Ctf19c/CCAN influences the timing of kinetochore assem...

journal_title：eLife

authors： Hinshaw SM,Harrison SC

更新日期：2019-02-14 00:00:00

abstract：:The neuronal DNA-/RNA-binding protein Pur-alpha is a transcription regulator and core factor for mRNA localization. Pur-alpha-deficient mice die after birth with pleiotropic neuronal defects. Here, we report the crystal structure of the DNA-/RNA-binding domain of Pur-alpha in complex with ssDNA. It reveals base-specif...

journal_title：eLife

authors： Weber J,Bao H,Hartlmüller C,Wang Z,Windhager A,Janowski R,Madl T,Jin P,Niessing D

更新日期：2016-01-08 00:00:00

abstract：:Viral replicase recruitment and long-range RNA interactions are essential for RNA virus replication, yet the mechanism of their interplay remains elusive. Flaviviruses include numerous important human pathogens, e.g., dengue virus (DENV) and Zika virus (ZIKV). Here, we revealed a highly conserved, conformation-tunable...

journal_title：eLife

authors： Liu ZY,Li XF,Jiang T,Deng YQ,Ye Q,Zhao H,Yu JY,Qin CF

更新日期：2016-10-01 00:00:00

abstract：:Combining clonal analysis with a computational agent based model, we investigate how tissue-specific stem cells for neural retina (NR) and retinal pigmented epithelium (RPE) of the teleost medaka (Oryzias latipes) coordinate their growth rates. NR cell division timing is less variable, consistent with an upstream role...

journal_title：eLife

authors： Tsingos E,Höckendorf B,Sütterlin T,Kirchmaier S,Grabe N,Centanin L,Wittbrodt J

更新日期：2019-03-26 00:00:00

abstract：:Epicardial cells on the heart's surface give rise to coronary artery smooth muscle cells (caSMCs) located deep in the myocardium. However, the differentiation steps between epicardial cells and caSMCs are unknown as are the final maturation signals at coronary arteries. Here, we use clonal analysis and lineage tracing...

journal_title：eLife

authors： Volz KS,Jacobs AH,Chen HI,Poduri A,McKay AS,Riordan DP,Kofler N,Kitajewski J,Weissman I,Red-Horse K

更新日期：2015-10-19 00:00:00

abstract：:INAD assembles key enzymes of the Drosophila compound eye photo-transduction pathway into a supramolecular complex, supporting efficient and fast light signaling. However, the molecular mechanism that governs the interaction between INAD and NORPA (phospholipase Cβ, PLCβ), a key step for the fast kinetics of the light...

journal_title：eLife

authors： Ye F,Huang Y,Li J,Ma Y,Xie C,Liu Z,Deng X,Wan J,Xue T,Liu W,Zhang M

更新日期：2018-12-10 00:00:00

abstract：:Using a common analysis pipeline to compare data from three major lineages of complex eukaryotes reveals that transcription seems to evolve at a common rate. ...

journal_title：eLife

authors： Odom DT

更新日期：2016-02-11 00:00:00

abstract：:Dietary magnesium (Mg2+) supplementation can enhance memory in young and aged rats. Memory-enhancing capacity was largely ascribed to increases in hippocampal synaptic density and elevated expression of the NR2B subunit of the NMDA-type glutamate receptor. Here we show that Mg2+ feeding also enhances long-term memory ...

journal_title：eLife

authors： Wu Y,Funato Y,Meschi E,Jovanoski KD,Miki H,Waddell S

更新日期：2020-11-26 00:00:00

abstract：:Predicting and constraining RNA virus evolution require understanding the molecular factors that define the mutational landscape accessible to these pathogens. RNA viruses typically have high mutation rates, resulting in frequent production of protein variants with compromised biophysical properties. Their evolution i...

journal_title：eLife

authors： Phillips AM,Gonzalez LO,Nekongo EE,Ponomarenko AI,McHugh SM,Butty VL,Levine SS,Lin YS,Mirny LA,Shoulders MD

更新日期：2017-09-26 00:00:00

abstract：:Two distinct mechanisms for primordial germ cell (PGC) specification are observed within Bilatera: early determination by maternal factors or late induction by zygotic cues. Here we investigate the molecular basis for PGC specification in Nematostella, a representative pre-bilaterian animal where PGCs arise as paired ...

journal_title：eLife

authors： Chen CY,McKinney SA,Ellington LR,Gibson MC

更新日期：2020-09-24 00:00:00

abstract：:Using knowledge of the structure of the world to infer value is at the heart of model-based reasoning and relies on a circuit that includes the orbitofrontal cortex (OFC). Some accounts link this to the representation of biological significance or value by neurons in OFC, while other models focus on the representation...

journal_title：eLife

authors： Sadacca BF,Wied HM,Lopatina N,Saini GK,Nemirovsky D,Schoenbaum G

更新日期：2018-03-07 00:00:00

abstract：:Two gene variants provide different levels of protection against sleeping sickness, but this comes with an increased risk of developing chronic kidney disease. ...

journal_title：eLife

authors： Kruzel-Davila E,Skorecki K

更新日期：2017-07-03 00:00:00

abstract：:Human pluripotent stem cell (hPSC)-derived neural organoids display unprecedented emergent properties. Yet in contrast to the singular neuroepithelial tube from which the entire central nervous system (CNS) develops in vivo, current organoid protocols yield tissues with multiple neuroepithelial units, a.k.a. neural ro...

journal_title：eLife

authors： Knight GT,Lundin BF,Iyer N,Ashton LM,Sethares WA,Willett RM,Ashton RS

更新日期：2018-10-29 00:00:00