Interfering with transmission.

abstract：:Organisms possessing genetic codes with unassigned codons raise the question of how cellular machinery resolves such codons and how this could impact horizontal gene transfer. Here, we use a genomically recoded Escherichia coli to examine how organisms address translation at unassigned UAG codons, which obstruct propa...

journal_title：eLife

authors： Ma NJ,Hemez CF,Barber KW,Rinehart J,Isaacs FJ

更新日期：2018-10-30 00:00:00

abstract：:Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that degrades aberrant mRNAs and also regulates the expression of a wide range of physiological transcripts. RUVBL1 and RUVBL2 AAA-ATPases form an hetero-hexameric ring that is part of several macromolecular complexes such as INO80, SWR1, and R2TP. Interesti...

journal_title：eLife

authors： López-Perrote A,Hug N,González-Corpas A,Rodríguez CF,Serna M,García-Martín C,Boskovic J,Fernandez-Leiro R,Caceres JF,Llorca O

更新日期：2020-11-18 00:00:00

abstract：:Arm-amputation involves two powerful drivers for brain plasticity-sensory deprivation and altered use. However, research has largely focused on sensory deprivation and maladaptive change. Here we show that adaptive patterns of limb usage after amputation drive cortical plasticity. We report that individuals with conge...

journal_title：eLife

authors： Makin TR,Cramer AO,Scholz J,Hahamy A,Henderson Slater D,Tracey I,Johansen-Berg H

更新日期：2013-11-12 00:00:00

abstract：:We analyzed 2898 scientific papers published between 1995 and 2017 in which two or more authors shared the first author position. For papers in which the first and second authors made equal contributions, mixed-gender combinations were most frequent, followed by male-male and then female-female author combinations. Fo...

journal_title：eLife

authors： Broderick NA,Casadevall A

更新日期：2019-01-29 00:00:00

abstract：:Skeletal muscle degeneration is a complication arising from a variety of chronic diseases including advanced cancer. Pro-inflammatory cytokine TNF-α plays a pivotal role in mediating cancer-related skeletal muscle degeneration. Here, we show a novel function for retinoblastoma protein (Rb), where Rb causes sarcomeric ...

journal_title：eLife

authors： Araki K,Kawauchi K,Hirata H,Yamamoto M,Taya Y

更新日期：2013-12-03 00:00:00

abstract：:Lipoprotein lipase (LPL) undergoes spontaneous inactivation via global unfolding and this unfolding is prevented by GPIHBP1 (Mysling et al., 2016). We now show: (1) that ANGPTL4 inactivates LPL by catalyzing the unfolding of its hydrolase domain; (2) that binding to GPIHBP1 renders LPL largely refractory to this inhib...

journal_title：eLife

authors： Mysling S,Kristensen KK,Larsson M,Kovrov O,Bensadouen A,Jørgensen TJ,Olivecrona G,Young SG,Ploug M

更新日期：2016-12-08 00:00:00

abstract：:Amyloid plaques, consisting of deposited beta-amyloid (Aβ), are a neuropathological hallmark of Alzheimer's Disease (AD). Cerebral vessels play a major role in AD, as Aβ is cleared from the brain by pathways involving the cerebrovasculature, most AD patients have cerebrovascular amyloid (cerebral amyloid angiopathy (C...

journal_title：eLife

authors： Robert J,Button EB,Yuen B,Gilmour M,Kang K,Bahrabadi A,Stukas S,Zhao W,Kulic I,Wellington CL

更新日期：2017-10-10 00:00:00

abstract：:Even when we are at rest, our spinal cords show spontaneous, yet well organised, fluctuations of activity that might reflect sensory and motor networks. ...

journal_title：eLife

authors： Eippert F,Tracey I

更新日期：2014-08-05 00:00:00

abstract：:New ages for flowstone, sediments and fossil bones from the Dinaledi Chamber are presented. We combined optically stimulated luminescence dating of sediments with U-Th and palaeomagnetic analyses of flowstones to establish that all sediments containing Homo naledi fossils can be allocated to a single stratigraphic ent...

journal_title：eLife

authors： Dirks PH,Roberts EM,Hilbert-Wolf H,Kramers JD,Hawks J,Dosseto A,Duval M,Elliott M,Evans M,Grün R,Hellstrom J,Herries AI,Joannes-Boyau R,Makhubela TV,Placzek CJ,Robbins J,Spandler C,Wiersma J,Woodhead J,Berger LR

更新日期：2017-05-09 00:00:00

abstract：:Recent advances in single-cell transcriptomics techniques have opened the door to the study of gene regulatory networks (GRNs) at the single-cell level. Here, we studied the GRNs controlling the emergence of hematopoietic stem and progenitor cells from mouse embryonic endothelium using a combination of single-cell tra...

journal_title：eLife

authors： Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

更新日期：2018-03-20 00:00:00

abstract：:Studies in different animal model systems have revealed the impact of odors on immune cells; however, any understanding on why and how odors control cellular immunity remained unclear. We find that Drosophila employ an olfactory-immune cross-talk to tune a specific cell type, the lamellocytes, from hematopoietic-proge...

journal_title：eLife

authors： Madhwal S,Shin M,Kapoor A,Goyal M,Joshi MK,Ur Rehman PM,Gor K,Shim J,Mukherjee T

更新日期：2020-12-29 00:00:00

abstract：:We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B suggests that the N...

journal_title：eLife

authors： Padavannil A,Sarkar P,Kim SJ,Cagatay T,Jiou J,Brautigam CA,Tomchick DR,Sali A,D'Arcy S,Chook YM

更新日期：2019-03-11 00:00:00

abstract：:Synaptic positions underlie precise circuit connectivity. Synaptic positions can be established during embryogenesis and sustained during growth. The mechanisms that sustain synaptic specificity during allometric growth are largely unknown. We performed forward genetic screens in C. elegans for regulators of this proc...

journal_title：eLife

authors： Fan J,Ji T,Wang K,Huang J,Wang M,Manning L,Dong X,Shi Y,Zhang X,Shao Z,Colón-Ramos DA

更新日期：2020-04-07 00:00:00

abstract：:Rapid and flexible interpretation of conflicting sensory inputs in the context of current goals is a critical component of cognitive control that is orchestrated by frontal cortex. The relative roles of distinct subregions within frontal cortex are poorly understood. To examine the dynamics underlying cognitive contro...

journal_title：eLife

authors： Tang H,Yu HY,Chou CC,Crone NE,Madsen JR,Anderson WS,Kreiman G

更新日期：2016-02-18 00:00:00

abstract：:PKMζ is a persistently active PKC isoform proposed to maintain late-LTP and long-term memory. But late-LTP and memory are maintained without PKMζ in PKMζ-null mice. Two hypotheses can account for these findings. First, PKMζ is unimportant for LTP or memory. Second, PKMζ is essential for late-LTP and long-term memory i...

journal_title：eLife

authors： Tsokas P,Hsieh C,Yao Y,Lesburguères E,Wallace EJC,Tcherepanov A,Jothianandan D,Hartley BR,Pan L,Rivard B,Farese RV,Sajan MP,Bergold PJ,Hernández AI,Cottrell JE,Shouval HZ,Fenton AA,Sacktor TC

更新日期：2016-05-17 00:00:00

abstract：:The receptor protein Dectin-1 recognizes structures found on cancerous cells, and then triggers an anti-tumor immune response. ...

journal_title：eLife

authors： Okabe Y,Medzhitov R

更新日期：2014-09-24 00:00:00

abstract：:While specific mutations allow organisms to adapt to stressful environments, most changes in an organism's DNA negatively impact fitness. The mutation rate is therefore strictly regulated and often considered a slowly-evolving parameter. In contrast, we demonstrate an unexpected flexibility in cellular mutation rates ...

journal_title：eLife

authors： Swings T,Van den Bergh B,Wuyts S,Oeyen E,Voordeckers K,Verstrepen KJ,Fauvart M,Verstraeten N,Michiels J

更新日期：2017-05-02 00:00:00

abstract：:Glucocorticoid receptors (GR) have diverse functions relevant to maintenance of homeostasis and adaptation to environmental challenges. Understanding the importance of tissue-specific GR function in physiology and behavior has been hampered by near-ubiquitous localization in brain and body. Here we use CRISPR/Cas9 gen...

journal_title：eLife

authors： Scheimann JR,Moloney RD,Mahbod P,Morano RL,Fitzgerald M,Hoskins O,Packard BA,Cotella EM,Hu YC,Herman JP

更新日期：2019-07-22 00:00:00

abstract：: ...

journal_title：eLife

authors： MacKay CE,Leo MD,Fernández-Peña C,Hasan R,Yin W,Mata-Daboin A,Bulley S,Gammons J,Mancarella S,Jaggar JH

更新日期：2020-06-30 00:00:00

abstract：:Protein synthesis ends when a ribosome reaches an mRNA stop codon. Release factors (RFs) decode the stop codon, hydrolyze peptidyl-tRNA to release the nascent protein, and then dissociate to allow ribosome recycling. To visualize termination by RF2, we resolved a cryo-EM ensemble of E. coli 70S•RF2 structures at up to...

journal_title：eLife

authors： Svidritskiy E,Demo G,Loveland AB,Xu C,Korostelev AA

更新日期：2019-09-12 00:00:00

abstract：:How Mycobacterium tuberculosis (Mtb) rewires macrophage energy metabolism to facilitate survival is poorly characterized. Here, we used extracellular flux analysis to simultaneously measure the rates of glycolysis and respiration in real time. Mtb infection induced a quiescent energy phenotype in human monocyte-derive...

journal_title：eLife

authors： Cumming BM,Addicott KW,Adamson JH,Steyn AJ

更新日期：2018-11-16 00:00:00

abstract：:Sex-specific synaptic connectivity is beginning to emerge as a remarkable, but little explored feature of animal brains. We describe here a novel mechanism that promotes sexually dimorphic neuronal function and synaptic connectivity in the nervous system of the nematode Caenorhabditis elegans. We demonstrate that a ph...

journal_title：eLife

authors： Bayer EA,Stecky RC,Neal L,Katsamba PS,Ahlsen G,Balaji V,Hoppe T,Shapiro L,Oren-Suissa M,Hobert O

更新日期：2020-10-06 00:00:00

abstract：:Neural circuits that control copulation in male flies have been identified. ...

journal_title：eLife

authors： Levine J

更新日期：2016-11-15 00:00:00

abstract：:Unrepaired DNA damage during embryonic development can be potentially inherited by a large population of cells. However, the quality control mechanisms that minimize the contribution of damaged cells to developing embryos remain poorly understood. Here, we uncovered an ATR- and CHK1-mediated transcriptional response t...

journal_title：eLife

authors： Atashpaz S,Samadi Shams S,Gonzalez JM,Sebestyén E,Arghavanifard N,Gnocchi A,Albers E,Minardi S,Faga G,Soffientini P,Allievi E,Cancila V,Bachi A,Fernández-Capetillo Ó,Tripodo C,Ferrari F,López-Contreras AJ,Costanzo V

更新日期：2020-03-12 00:00:00

abstract：:Cerebral cortical architecture at birth encodes regionally differential dendritic arborization and synaptic formation. It underlies behavioral emergence of 2-year-olds. Brain changes in 0-2 years are most dynamic across the lifespan. Effective prediction of future behavior with brain microstructure at birth will revea...

journal_title：eLife

authors： Ouyang M,Peng Q,Jeon T,Heyne R,Chalak L,Huang H

更新日期：2020-12-22 00:00:00

abstract：:Little is known about venom in young developmental stages of animals. The appearance of toxins and stinging cells during early embryonic stages in the sea anemone Nematostella vectensis suggests that venom is already expressed in eggs and larvae of this species. Here, we harness transcriptomic, biochemical and transge...

journal_title：eLife

authors： Columbus-Shenkar YY,Sachkova MY,Macrander J,Fridrich A,Modepalli V,Reitzel AM,Sunagar K,Moran Y

更新日期：2018-02-09 00:00:00

abstract：:Cytoplasmic dyneins are motor proteins in the AAA+ superfamily that transport cellular cargos toward microtubule minus-ends. Recently, ciliobrevins were reported as selective cell-permeable inhibitors of cytoplasmic dyneins. As is often true for first-in-class inhibitors, the use of ciliobrevins has in part been limit...

journal_title：eLife

authors： Steinman JB,Santarossa CC,Miller RM,Yu LS,Serpinskaya AS,Furukawa H,Morimoto S,Tanaka Y,Nishitani M,Asano M,Zalyte R,Ondrus AE,Johnson AG,Ye F,Nachury MV,Fukase Y,Aso K,Foley MA,Gelfand VI,Chen JK,Carter AP,Kapo

更新日期：2017-05-19 00:00:00

abstract：:Pervasive expression of circular RNA is a recently discovered feature of eukaryotic gene expression programs, yet its function remains largely unknown. The presumed biogenesis of these RNAs involves a non-canonical 'backsplicing' event. Recent studies in mammalian cell culture posit that backsplicing is facilitated by...

journal_title：eLife

authors： Barrett SP,Wang PL,Salzman J

更新日期：2015-06-09 00:00:00

abstract：:Sex-optimal diets have different effects on gene expression in female and male flies. ...

journal_title：eLife

authors： Flatt T

更新日期：2019-10-01 00:00:00

abstract：:Evolution persistently undermines vector control programs through insecticide resistance. Here we propose a novel strategy which instead exploits evolution to generate and sustain new control tools. Effective spatial repellents are needed to keep vectors out of houses. Our approach generates such new repellents by com...

journal_title：eLife

authors： Lynch PA,Boots M

更新日期：2016-10-25 00:00:00