相关文献
eLife文献大全abstract::The nematode Caenorhabditis elegans possesses a simple embryonic nervous system with few enough neurons that the growth of each cell could be followed to provide a systems-level view of development. However, studies of single cell development have largely been conducted in fixed or pre-twitching live embryos, because ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10070
更新日期:2015-12-03 00:00:00
abstract::AMPARs control fast synaptic communication between neurons and their function relies on auxiliary subunits, which importantly modulate channel properties. Although it has been suggested that AMPARs can bind to TARPs with variable stoichiometry, little is known about the effect that this stoichiometry exerts on certain...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53946
更新日期:2020-05-26 00:00:00
abstract::Existing in vitro models of human skeletal muscle cannot recapitulate the organization and function of native muscle, limiting their use in physiological and pharmacological studies. Here, we demonstrate engineering of electrically and chemically responsive, contractile human muscle tissues ('myobundles') using primar...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04885
更新日期:2015-01-09 00:00:00
abstract::The Hippo-YAP pathway is a central regulator of cell contact inhibition, proliferation and death. There are conflicting reports regarding the role of Angiomotin (Amot) in regulating this pathway. While some studies suggest a YAP-inhibitory function other studies indicate Amot is required for YAP activity. Here, we des...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23966
更新日期:2017-05-03 00:00:00
abstract::Bacterial flagella mediate host-microbe interactions through tissue tropism during colonization, as well as by activating immune responses. The flagellar shaft of some bacteria, including several human pathogens, is encased in a membranous sheath of unknown function. While it has been hypothesized that the sheath may ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01579
更新日期:2014-03-04 00:00:00
abstract::C-type inactivation of potassium channels fine-tunes the electrical signaling in excitable cells through an internal timing mechanism that is mediated by a hydrogen bond network in the channels' selectively filter. Previously, we used nonsense suppression to highlight the role of the conserved Trp434-Asp447 indole hyd...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18976
更新日期:2016-10-06 00:00:00
abstract::Microtubules are nucleated from specific locations at precise times in the cell cycle. However, the factors that constitute these microtubule nucleation pathways and their mode of action still need to be identified. Using purified Xenopus laevis proteins we biochemically reconstitute branching microtubule nucleation, ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49797
更新日期:2020-01-14 00:00:00
abstract::Peptidoglycan (PG) is the main component of bacterial cell walls and the target for many antibiotics. PG biosynthesis is tightly coordinated with cell wall growth and turnover, and many of these control activities depend upon PASTA-domain containing eukaryotic-like serine/threonine protein kinases (PASTA-eSTK) that se...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56048
更新日期:2020-05-29 00:00:00
abstract::The NuRD complex subunit CHD4 is essential for fusion-positive rhabdomyosarcoma (FP-RMS) survival, but the mechanisms underlying this dependency are not understood. Here, a NuRD-specific CRISPR screen demonstrates that FP-RMS is particularly sensitive to CHD4 amongst the NuRD members. Mechanistically, NuRD complex con...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54993
更新日期:2020-08-03 00:00:00
abstract::Dietary restriction increases the longevity of many organisms, but the cell signaling and organellar mechanisms underlying this capability are unclear. We demonstrate that to permit long-term survival in response to sudden glucose depletion, yeast cells activate lipid-droplet (LD) consumption through micro-lipophagy (...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21690
更新日期:2017-04-10 00:00:00
abstract::Spatial and temporal cues are required to specify neuronal diversity, but how these cues are integrated in neural progenitors remains unknown. Drosophila progenitors (neuroblasts) are a good model: they are individually identifiable with relevant spatial and temporal transcription factors known. Here we test whether s...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44036
更新日期:2019-01-29 00:00:00
abstract::During cell locomotion and endocytosis, membrane-tethered WASP proteins stimulate actin filament nucleation by the Arp2/3 complex. This process generates highly branched arrays of filaments that grow toward the membrane to which they are tethered, a conflict that seemingly would restrict filament growth. Using three-c...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01008
更新日期:2013-09-03 00:00:00
abstract::Cognitive functions such as working memory (WM) are emergent properties of large-scale network interactions. Synchronisation of oscillatory activity might contribute to WM by enabling the coordination of long-range processes. However, causal evidence for the way oscillatory activity shapes network dynamics and behavio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22001
更新日期:2017-03-14 00:00:00
abstract::The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21012
更新日期:2017-03-13 00:00:00
abstract::Orphans are genes restricted to a single phylogenetic lineage and emerge at high rates. While this predicts an accumulation of genes, the gene number has remained remarkably constant through evolution. This paradox has not yet been resolved. Because orphan genes have been mainly analyzed over long evolutionary time sc...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01311
更新日期:2014-02-19 00:00:00
abstract::Striatal dysfunction plays an important role in dystonia, but the striatal cell types that contribute to abnormal movements are poorly defined. We demonstrate that conditional deletion of the DYT1 dystonia protein torsinA in embryonic progenitors of forebrain cholinergic and GABAergic neurons causes dystonic-like twis...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.08352
更新日期:2015-06-08 00:00:00
abstract::SUMO-modification of nuclear proteins has profound effects on gene expression. However, non-toxic chemical tools that modulate sumoylation in cells are lacking. Here, to identify small molecule sumoylation inhibitors we developed a cell-based screen that focused on the well-sumoylated substrate, human Liver Receptor H...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.09003
更新日期:2015-12-11 00:00:00
abstract::Single-molecule imaging experiments have shed new light on the methods used by the enzyme RecA to align single- and double-stranded DNA so that double-strand breaks can be repaired. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.00347
更新日期:2012-12-13 00:00:00
abstract::The rhythm generating network for breathing must continuously adjust to changing metabolic and behavioral demands. Here, we examined network-based mechanisms in the mouse preBötzinger complex using substance P, a potent excitatory modulator of breathing frequency and stability, as a tool to dissect network properties ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51350
更新日期:2019-12-05 00:00:00
abstract::Two distinct mechanisms for primordial germ cell (PGC) specification are observed within Bilatera: early determination by maternal factors or late induction by zygotic cues. Here we investigate the molecular basis for PGC specification in Nematostella, a representative pre-bilaterian animal where PGCs arise as paired ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54573
更新日期:2020-09-24 00:00:00
abstract::Animals host multi-species microbial communities (microbiomes) whose properties may result from inter-species interactions; however, current understanding of host-microbiome interactions derives mostly from studies in which elucidation of microbe-microbe interactions is difficult. In exploring how Drosophila melanogas...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18855
更新日期:2017-01-09 00:00:00
abstract::Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans after silicone oil (S...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.45881
更新日期:2019-05-15 00:00:00
abstract::LAMP1 and LAMP2 proteins are highly abundant, ubiquitous, mammalian proteins that line the lysosome limiting membrane, and protect it from lysosomal hydrolase action. LAMP2 deficiency causes Danon's disease, an X-linked hypertrophic cardiomyopathy. LAMP2 is needed for chaperone-mediated autophagy, and its expression i...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21635
更新日期:2016-09-24 00:00:00
abstract::Animals seek out relevant information by moving through a dynamic world, but sensory systems are usually studied under highly constrained and passive conditions that may not probe important dimensions of the neural code. Here, we explored neural coding in the barrel cortex of head-fixed mice that tracked walls with th...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12559
更新日期:2015-12-23 00:00:00
abstract::Swi2/Snf2 ATPases remodel substrates such as nucleosomes and transcription complexes to control a wide range of DNA-associated processes, but detailed structural information on the ATP-dependent remodeling reactions is largely absent. The single subunit remodeler Mot1 (modifier of transcription 1) dissociates TATA box...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07432
更新日期:2015-08-10 00:00:00
abstract::Drug treatment of 3D cancer spheroids more accurately reflects in vivo therapeutic responses compared to adherent culture studies. In EGFR-mutated lung adenocarcinoma, EGFR-TKIs show enhanced efficacy in spheroid cultures. Simultaneous inhibition of multiple parallel RTKs further enhances EGFR-TKI effectiveness. We sh...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58204
更新日期:2020-09-08 00:00:00
abstract::How different helicase families with a conserved catalytic 'helicase core' evolved to function on varied RNA and DNA substrates by diverse mechanisms remains unclear. In this study, we used Mss116, a yeast DEAD-box protein that utilizes ATP to locally unwind dsRNA, to investigate helicase specificity and mechanism. Ou...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04630
更新日期:2014-12-12 00:00:00
abstract::Embryonic polarity of invertebrates, amphibians and fish is specified largely by maternal determinants, which fixes cell fates early in development. In contrast, amniote embryos remain plastic and can form multiple individuals until gastrulation. How is their polarity determined? In the chick embryo, the earliest know...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03743
更新日期:2014-12-11 00:00:00
abstract::Our understanding of nonlinear stimulus transformations by neural circuits is hindered by the lack of comprehensive yet interpretable computational modeling frameworks. Here, we propose a data-driven approach based on deep neural networks to directly model arbitrarily nonlinear stimulus-response mappings. Reformulatin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53445
更新日期:2020-06-26 00:00:00
abstract::Experiments on mitochondrial DNA in worms highlight that cheating does not always pay off. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.62222
更新日期:2020-09-22 00:00:00