Regulation of localization and function of the transcriptional co-activator YAP by angiomotin.


:The Hippo-YAP pathway is a central regulator of cell contact inhibition, proliferation and death. There are conflicting reports regarding the role of Angiomotin (Amot) in regulating this pathway. While some studies suggest a YAP-inhibitory function other studies indicate Amot is required for YAP activity. Here, we describe an Amot-dependent complex comprised of Amot, YAP and Merlin. The phosphorylation of Amot at Serine 176 shifts localization of this complex to the plasma membrane, where it associates with the tight-junction proteins Pals1/PATJ and E-cadherin. Conversely, hypophosphorylated Amot shifts localization of the complex to the nucleus, where it facilitates the association of YAP and TEAD, induces transcriptional activation of YAP target genes and promotes YAP-dependent cell proliferation. We propose that phosphorylation of AmotS176 is a critical post-translational modification that suppresses YAP's ability to promote cell proliferation and tumorigenesis by altering the subcellular localization of an essential YAP co-factor.






Moleirinho S,Hoxha S,Mandati V,Curtale G,Troutman S,Ehmer U,Kissil JL




Has Abstract


2017-05-03 00:00:00








  • Engineering induction of singular neural rosette emergence within hPSC-derived tissues.

    abstract::Human pluripotent stem cell (hPSC)-derived neural organoids display unprecedented emergent properties. Yet in contrast to the singular neuroepithelial tube from which the entire central nervous system (CNS) develops in vivo, current organoid protocols yield tissues with multiple neuroepithelial units, a.k.a. neural ro...


    pub_type: 杂志文章


    authors: Knight GT,Lundin BF,Iyer N,Ashton LM,Sethares WA,Willett RM,Ashton RS

    更新日期:2018-10-29 00:00:00

  • An unexpected role for the yeast nucleotide exchange factor Sil1 as a reductant acting on the molecular chaperone BiP.

    abstract::Unfavorable redox conditions in the endoplasmic reticulum (ER) can decrease the capacity for protein secretion, altering vital cell functions. While systems to manage reductive stress are well-established, how cells cope with an overly oxidizing ER remains largely undefined. In previous work (Wang et al., 2014), we de...


    pub_type: 杂志文章


    authors: Siegenthaler KD,Pareja KA,Wang J,Sevier CS

    更新日期:2017-03-03 00:00:00

  • Structural basis of nucleic-acid recognition and double-strand unwinding by the essential neuronal protein Pur-alpha.

    abstract::The neuronal DNA-/RNA-binding protein Pur-alpha is a transcription regulator and core factor for mRNA localization. Pur-alpha-deficient mice die after birth with pleiotropic neuronal defects. Here, we report the crystal structure of the DNA-/RNA-binding domain of Pur-alpha in complex with ssDNA. It reveals base-specif...


    pub_type: 杂志文章


    authors: Weber J,Bao H,Hartlmüller C,Wang Z,Windhager A,Janowski R,Madl T,Jin P,Niessing D

    更新日期:2016-01-08 00:00:00

  • KRAS-dependent sorting of miRNA to exosomes.

    abstract::Mutant KRAS colorectal cancer (CRC) cells release protein-laden exosomes that can alter the tumor microenvironment. To test whether exosomal RNAs also contribute to changes in gene expression in recipient cells, and whether mutant KRAS might regulate the composition of secreted microRNAs (miRNAs), we compared small RN...


    pub_type: 杂志文章


    authors: Cha DJ,Franklin JL,Dou Y,Liu Q,Higginbotham JN,Demory Beckler M,Weaver AM,Vickers K,Prasad N,Levy S,Zhang B,Coffey RJ,Patton JG

    更新日期:2015-07-01 00:00:00

  • Inhibition of protein synthesis in M1 of monkeys disrupts performance of sequential movements guided by memory.

    abstract::The production of action sequences is a fundamental aspect of motor skills. To examine whether primary motor cortex (M1) is involved in maintenance of sequential movements, we trained two monkeys (Cebus apella) to perform two sequential reaching tasks. In one task, sequential movements were instructed by visual cues, ...


    pub_type: 杂志文章


    authors: Ohbayashi M

    更新日期:2020-02-10 00:00:00

  • Large-scale remodeling of a repressed exon ribonucleoprotein to an exon definition complex active for splicing.

    abstract::Polypyrimidine-tract binding protein PTBP1 can repress splicing during the exon definition phase of spliceosome assembly, but the assembly steps leading to an exon definition complex (EDC) and how PTBP1 might modulate them are not clear. We found that PTBP1 binding in the flanking introns allowed normal U2AF and U1 sn...


    pub_type: 杂志文章


    authors: Wongpalee SP,Vashisht A,Sharma S,Chui D,Wohlschlegel JA,Black DL

    更新日期:2016-11-24 00:00:00

  • Cascade of neural processing orchestrates cognitive control in human frontal cortex.

    abstract::Rapid and flexible interpretation of conflicting sensory inputs in the context of current goals is a critical component of cognitive control that is orchestrated by frontal cortex. The relative roles of distinct subregions within frontal cortex are poorly understood. To examine the dynamics underlying cognitive contro...


    pub_type: 杂志文章


    authors: Tang H,Yu HY,Chou CC,Crone NE,Madsen JR,Anderson WS,Kreiman G

    更新日期:2016-02-18 00:00:00

  • Embryonic transcription factor expression in mice predicts medial amygdala neuronal identity and sex-specific responses to innate behavioral cues.

    abstract::The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...


    pub_type: 杂志文章


    authors: Lischinsky JE,Sokolowski K,Li P,Esumi S,Kamal Y,Goodrich M,Oboti L,Hammond TR,Krishnamoorthy M,Feldman D,Huntsman M,Liu J,Corbin JG

    更新日期:2017-03-13 00:00:00

  • Molecular architecture of the 90S small subunit pre-ribosome.

    abstract::Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle...


    pub_type: 杂志文章


    authors: Sun Q,Zhu X,Qi J,An W,Lan P,Tan D,Chen R,Wang B,Zheng S,Zhang C,Chen X,Zhang W,Chen J,Dong MQ,Ye K

    更新日期:2017-02-28 00:00:00

  • Efficacy of β-lactam/β-lactamase inhibitor combination is linked to WhiB4-mediated changes in redox physiology of Mycobacterium tuberculosis.

    abstract::Mycobacterium tuberculosis (Mtb) expresses a broad-spectrum β-lactamase (BlaC) that mediates resistance to one of the highly effective antibacterials, β-lactams. Nonetheless, β-lactams showed mycobactericidal activity in combination with β-lactamase inhibitor, clavulanate (Clav). However, the mechanistic aspects of ho...


    pub_type: 杂志文章


    authors: Mishra S,Shukla P,Bhaskar A,Anand K,Baloni P,Jha RK,Mohan A,Rajmani RS,Nagaraja V,Chandra N,Singh A

    更新日期:2017-05-26 00:00:00

  • Tuning site-specific dynamics to drive allosteric activation in a pneumococcal zinc uptake regulator.

    abstract::MarR (multiple antibiotic resistance repressor) family proteins are bacterial repressors that regulate transcription in response to a wide range of chemical signals. Although specific features of MarR family function have been described, the role of atomic motions in MarRs remains unexplored thus limiting insights int...


    pub_type: 杂志文章


    authors: Capdevila DA,Huerta F,Edmonds KA,Le MT,Wu H,Giedroc DP

    更新日期:2018-10-17 00:00:00

  • Tracking the popularity and outcomes of all bioRxiv preprints.

    abstract::The growth of preprints in the life sciences has been reported widely and is driving policy changes for journals and funders, but little quantitative information has been published about preprint usage. Here, we report how we collected and analyzed data on all 37,648 preprints uploaded to, the largest biol...


    pub_type: 杂志文章


    authors: Abdill RJ,Blekhman R

    更新日期:2019-04-24 00:00:00

  • Distinct responses to reduplicated chromosomes require distinct Mad2 responses.

    abstract::Duplicating chromosomes once each cell cycle produces sister chromatid pairs, which separate accurately at anaphase. In contrast, reduplicating chromosomes without separation frequently produces polytene chromosomes, a barrier to accurate mitosis. Chromosome reduplication occurs in many contexts, including: polytene t...


    pub_type: 杂志文章


    authors: Stormo BM,Fox DT

    更新日期:2016-05-09 00:00:00

  • Human biallelic MFN2 mutations induce mitochondrial dysfunction, upper body adipose hyperplasia, and suppression of leptin expression.

    abstract::MFN2 encodes mitofusin 2, a membrane-bound mediator of mitochondrial membrane fusion and inter-organelle communication. MFN2 mutations cause axonal neuropathy, with associated lipodystrophy only occasionally noted, however homozygosity for the p.Arg707Trp mutation was recently associated with upper body adipose overgr...


    pub_type: 杂志文章


    authors: Rocha N,Bulger DA,Frontini A,Titheradge H,Gribsholt SB,Knox R,Page M,Harris J,Payne F,Adams C,Sleigh A,Crawford J,Gjesing AP,Bork-Jensen J,Pedersen O,Barroso I,Hansen T,Cox H,Reilly M,Rossor A,Brown RJ,Taylor SI

    更新日期:2017-04-19 00:00:00

  • Mechanical inhibition of isolated Vo from V/A-ATPase for proton conductance.

    abstract::V-ATPase is an energy converting enzyme, coupling ATP hydrolysis/synthesis in the hydrophilic V1 domain, with proton flow through the Vo membrane domain, via rotation of the central rotor complex relative to the surrounding stator apparatus. Upon dissociation from the V1 domain, the Vo domain of the eukaryotic V-ATPas...


    pub_type: 杂志文章


    authors: Kishikawa JI,Nakanishi A,Furuta A,Kato T,Namba K,Tamakoshi M,Mitsuoka K,Yokoyama K

    更新日期:2020-07-08 00:00:00

  • Coronary arterial development is regulated by a Dll4-Jag1-EphrinB2 signaling cascade.

    abstract::Coronaries are essential for myocardial growth and heart function. Notch is crucial for mouse embryonic angiogenesis, but its role in coronary development remains uncertain. We show Jag1, Dll4 and activated Notch1 receptor expression in sinus venosus (SV) endocardium. Endocardial Jag1 removal blocks SV capillary sprou...


    pub_type: 杂志文章


    authors: Travisano SI,Oliveira VL,Prados B,Grego-Bessa J,Piñeiro-Sabarís R,Bou V,Gómez MJ,Sánchez-Cabo F,MacGrogan D,de la Pompa JL

    更新日期:2019-12-04 00:00:00

  • Decisions, decisions.

    abstract::Journals are exploring new approaches to peer review in order to reduce bias, increase transparency and respond to author preferences. Funders are also getting involved. ...


    pub_type: 杂志文章


    authors: Rodgers P

    更新日期:2017-09-29 00:00:00

  • The novel lncRNA lnc-NR2F1 is pro-neurogenic and mutated in human neurodevelopmental disorders.

    abstract::Long noncoding RNAs (lncRNAs) have been shown to act as important cell biological regulators including cell fate decisions but are often ignored in human genetics. Combining differential lncRNA expression during neuronal lineage induction with copy number variation morbidity maps of a cohort of children with autism sp...


    pub_type: 杂志文章


    authors: Ang CE,Ma Q,Wapinski OL,Fan S,Flynn RA,Lee QY,Coe B,Onoguchi M,Olmos VH,Do BT,Dukes-Rimsky L,Xu J,Tanabe K,Wang L,Elling U,Penninger JM,Zhao Y,Qu K,Eichler EE,Srivastava A,Wernig M,Chang HY

    更新日期:2019-01-10 00:00:00

  • Excitatory and inhibitory synapse reorganization immediately after critical sensory experience in a vocal learner.

    abstract::Excitatory and inhibitory synapses are the brain's most abundant synapse types. However, little is known about their formation during critical periods of motor skill learning, when sensory experience defines a motor target that animals strive to imitate. In songbirds, we find that exposure to tutor song leads to elimi...


    pub_type: 杂志文章


    authors: Huang Z,Khaled HG,Kirschmann M,Gobes SM,Hahnloser RH

    更新日期:2018-10-25 00:00:00

  • Knockout of Slo2.2 enhances itch, abolishes KNa current, and increases action potential firing frequency in DRG neurons.

    abstract::Two mammalian genes, Kcnt1 and Kcnt2, encode pore-forming subunits of Na(+)-dependent K(+) (KNa) channels. Progress in understanding KNa channels has been hampered by the absence of specific tools and methods for rigorous KNa identification in native cells. Here, we report the genetic disruption of both Kcnt1 and Kcnt...


    pub_type: 杂志文章


    authors: Martinez-Espinosa PL,Wu J,Yang C,Gonzalez-Perez V,Zhou H,Liang H,Xia XM,Lingle CJ

    更新日期:2015-11-11 00:00:00

  • Ring-like oligomers of Synaptotagmins and related C2 domain proteins.

    abstract::We recently reported that the C2AB portion of Synaptotagmin 1 (Syt1) could self-assemble into Ca(2+)-sensitive ring-like oligomers on membranes, which could potentially regulate neurotransmitter release. Here we report that analogous ring-like oligomers assemble from the C2AB domains of other Syt isoforms (Syt2, Syt7,...


    pub_type: 杂志文章


    authors: Zanetti MN,Bello OD,Wang J,Coleman J,Cai Y,Sindelar CV,Rothman JE,Krishnakumar SS

    更新日期:2016-07-19 00:00:00

  • Odd-paired is a pioneer-like factor that coordinates with Zelda to control gene expression in embryos.

    abstract::Pioneer factors such as Zelda (Zld) help initiate zygotic transcription in Drosophila early embryos, but whether other factors support this dynamic process is unclear. Odd-paired (Opa), a zinc-finger transcription factor expressed at cellularization, controls the transition of genes from pair-rule to segmental pattern...


    pub_type: 评论,杂志文章


    authors: Koromila T,Gao F,Iwasaki Y,He P,Pachter L,Gergen JP,Stathopoulos A

    更新日期:2020-07-23 00:00:00

  • A pain-mediated neural signal induces relapse in murine autoimmune encephalomyelitis, a multiple sclerosis model.

    abstract::Although pain is a common symptom of various diseases and disorders, its contribution to disease pathogenesis is not well understood. Here we show using murine experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis (MS), that pain induces EAE relapse. Mechanistic analysis showed that pain indu...


    pub_type: 杂志文章


    authors: Arima Y,Kamimura D,Atsumi T,Harada M,Kawamoto T,Nishikawa N,Stofkova A,Ohki T,Higuchi K,Morimoto Y,Wieghofer P,Okada Y,Mori Y,Sakoda S,Saika S,Yoshioka Y,Komuro I,Yamashita T,Hirano T,Prinz M,Murakami M

    更新日期:2015-08-11 00:00:00

  • Light reintroduction after dark exposure reactivates plasticity in adults via perisynaptic activation of MMP-9.

    abstract::The sensitivity of ocular dominance to regulation by monocular deprivation is the canonical model of plasticity confined to a critical period. However, we have previously shown that visual deprivation through dark exposure (DE) reactivates critical period plasticity in adults. Previous work assumed that the eliminatio...


    pub_type: 杂志文章


    authors: Murase S,Lantz CL,Quinlan EM

    更新日期:2017-09-06 00:00:00

  • A robust activity marking system for exploring active neuronal ensembles.

    abstract::Understanding how the brain captures transient experience and converts it into long lasting changes in neural circuits requires the identification and investigation of the specific ensembles of neurons that are responsible for the encoding of each experience. We have developed a Robust Activity Marking (RAM) system th...


    pub_type: 杂志文章


    authors: Sørensen AT,Cooper YA,Baratta MV,Weng FJ,Zhang Y,Ramamoorthi K,Fropf R,LaVerriere E,Xue J,Young A,Schneider C,Gøtzsche CR,Hemberg M,Yin JC,Maier SF,Lin Y

    更新日期:2016-09-23 00:00:00

  • Structural basis for the activation of PLC-γ isozymes by phosphorylation and cancer-associated mutations.

    abstract::Direct activation of the human phospholipase C-γ isozymes (PLC-γ1, -γ2) by tyrosine phosphorylation is fundamental to the control of diverse biological processes, including chemotaxis, platelet aggregation, and adaptive immunity. In turn, aberrant activation of PLC-γ1 and PLC-γ2 is implicated in inflammation, autoimmu...


    pub_type: 杂志文章


    authors: Hajicek N,Keith NC,Siraliev-Perez E,Temple BR,Huang W,Zhang Q,Harden TK,Sondek J

    更新日期:2019-12-31 00:00:00

  • Down regulation of vestibular balance stabilizing mechanisms to enable transition between motor states.

    abstract::The neural control of transition between posture and movement encompasses the regulation of reflex-stabilizing mechanisms to enable motion. Optimal feedback theory suggests that such transitions require the disengagement of one motor control policy before the implementation of another. To test this possibility, we inv...


    pub_type: 杂志文章


    authors: Tisserand R,Dakin CJ,Van der Loos MH,Croft EA,Inglis TJ,Blouin JS

    更新日期:2018-07-10 00:00:00

  • Fungal effector Ecp6 outcompetes host immune receptor for chitin binding through intrachain LysM dimerization.

    abstract::While host immune receptors detect pathogen-associated molecular patterns to activate immunity, pathogens attempt to deregulate host immunity through secreted effectors. Fungi employ LysM effectors to prevent recognition of cell wall-derived chitin by host immune receptors, although the mechanism to compete for chitin...


    pub_type: 杂志文章


    authors: Sánchez-Vallet A,Saleem-Batcha R,Kombrink A,Hansen G,Valkenburg DJ,Thomma BP,Mesters JR

    更新日期:2013-07-02 00:00:00

  • Cell-cycle-gated feedback control mediates desensitization to interferon stimulation.

    abstract::Cells use molecular circuits to interpret and respond to extracellular cues, such as hormones and cytokines, which are often released in a temporally varying fashion. In this study, we combine microfluidics, time-lapse microscopy, and computational modeling to investigate how the type I interferon (IFN)-responsive reg...


    pub_type: 杂志文章


    authors: Mudla A,Jiang Y,Arimoto KI,Xu B,Rajesh A,Ryan AP,Wang W,Daugherty MD,Zhang DE,Hao N

    更新日期:2020-09-18 00:00:00

  • Building a functional connectome of the Drosophila central complex.

    abstract::The central complex is a highly conserved insect brain region composed of morphologically stereotyped neurons that arborize in distinctively shaped substructures. The region is implicated in a wide range of behaviors and several modeling studies have explored its circuit computations. Most studies have relied on assum...


    pub_type: 杂志文章


    authors: Franconville R,Beron C,Jayaraman V

    更新日期:2018-08-20 00:00:00