Abstract:
:Dietary restriction increases the longevity of many organisms, but the cell signaling and organellar mechanisms underlying this capability are unclear. We demonstrate that to permit long-term survival in response to sudden glucose depletion, yeast cells activate lipid-droplet (LD) consumption through micro-lipophagy (µ-lipophagy), in which fat is metabolized as an alternative energy source. AMP-activated protein kinase (AMPK) activation triggered this pathway, which required Atg14p. More gradual glucose starvation, amino acid deprivation or rapamycin did not trigger µ-lipophagy and failed to provide the needed substitute energy source for long-term survival. During acute glucose restriction, activated AMPK was stabilized from degradation and interacted with Atg14p. This prompted Atg14p redistribution from ER exit sites onto liquid-ordered vacuole membrane domains, initiating µ-lipophagy. Our findings that activated AMPK and Atg14p are required to orchestrate µ-lipophagy for energy production in starved cells is relevant for studies on aging and evolutionary survival strategies of different organisms.
journal_name
Elifejournal_title
eLifeauthors
Seo AY,Lau PW,Feliciano D,Sengupta P,Gros MAL,Cinquin B,Larabell CA,Lippincott-Schwartz Jdoi
10.7554/eLife.21690subject
Has Abstractpub_date
2017-04-10 00:00:00issn
2050-084Xjournal_volume
6pub_type
杂志文章相关文献
eLife文献大全abstract::In addition to their role in desensitization and internalization of G protein-coupled receptors (GPCRs), β-arrestins are essential scaffolds linking GPCRs to Erk1/2 signaling. However, their role in GPCR-operated Erk1/2 activation differs between GPCRs and the underlying mechanism remains poorly characterized. Here, w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23777
更新日期:2017-02-07 00:00:00
abstract::The twin-arginine protein translocation system (Tat) transports folded proteins across the bacterial cytoplasmic membrane and the thylakoid membranes of plant chloroplasts. The Tat transporter is assembled from multiple copies of the membrane proteins TatA, TatB, and TatC. We combine sequence co-evolution analysis, mo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20718
更新日期:2016-12-03 00:00:00
abstract::As the equipment needed to perform state-of-the-art research in many areas of biology becomes ever more expensive, Eve Marder worries that researchers in less wealthy institutions might be left behind. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01515
更新日期:2013-11-19 00:00:00
abstract::Innate lymphoid cells (ILCs) are recently identified lymphocytes that limit infection and promote tissue repair at mucosal surfaces. However, the pathways underlying ILC development remain unclear. Here we show that the transcription factor NFIL3 directs the development of a committed bone marrow precursor that differ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04406
更新日期:2014-10-13 00:00:00
abstract::Animals seek out relevant information by moving through a dynamic world, but sensory systems are usually studied under highly constrained and passive conditions that may not probe important dimensions of the neural code. Here, we explored neural coding in the barrel cortex of head-fixed mice that tracked walls with th...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12559
更新日期:2015-12-23 00:00:00
abstract::Motile ciliopathies are characterized by specific defects in cilia beating that result in chronic airway disease, subfertility, ectopic pregnancy, and hydrocephalus. While many patients harbor mutations in the dynein motors that drive cilia beating, the disease also results from mutations in so-called dynein axonemal ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.38497
更新日期:2018-12-18 00:00:00
abstract::Single-molecule experiments reveal the dynamics of transcription through a nucleosome with single-base-pair accuracy. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.50761
更新日期:2019-09-13 00:00:00
abstract::Using a clever combination of genetic and biochemical tools researchers have shown that a transcription factor called USF1 has a central role in determining how mutations of the Clock gene manifest themselves in the behaviour of different mouse strains. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.00659
更新日期:2013-04-09 00:00:00
abstract::An analysis of worldwide human genetic variation reveals the footprints of ancient changes in genomic mutation processes. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.27605
更新日期:2017-05-17 00:00:00
abstract::hIAPP fibrils are associated with Type-II Diabetes, but the link of hIAPP structure to islet cell death remains elusive. Here we observe that hIAPP fibrils are cytotoxic to cultured pancreatic β-cells, leading us to determine the structure and cytotoxicity of protein segments composing the amyloid spine of hIAPP. Usin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19273
更新日期:2017-01-03 00:00:00
abstract::Multiple lines of evidence suggest that functionally intact cerebello-hippocampal interactions are required for appropriate spatial processing. However, how the cerebellum anatomically and physiologically engages with the hippocampus to sustain such communication remains unknown. Using rabies virus as a retrograde tra...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41896
更新日期:2019-06-17 00:00:00
abstract::Eukaryotic kinetochores connect spindlemicrotubules to chromosomal centromeres. A group of proteins called the Ctf19 complex (Ctf19c) in yeast and the constitutive centromere associated network (CCAN) in other organisms creates the foundation of a kinetochore. The Ctf19c/CCAN influences the timing of kinetochore assem...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44239
更新日期:2019-02-14 00:00:00
abstract::Although activation/deactivation of specific brain regions has been shown to be predictive of successful memory encoding, the relationship between time-varying large-scale brain networks and fluctuations of memory encoding performance remains unclear. Here, we investigated time-varying functional connectivity patterns...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.32696
更新日期:2018-06-18 00:00:00
abstract::Gustatory pheromones play an essential role in shaping the behavior of many organisms. However, little is known about the processing of taste pheromones in higher order brain centers. Here, we describe a male-specific gustatory circuit in Drosophila that underlies the detection of the anti-aphrodisiac pheromone (3R,11...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.06914
更新日期:2015-06-17 00:00:00
abstract::Despite tremendous body form diversity in nature, bilaterian animals share common sets of developmental genes that display conserved expression patterns in the embryo. Among them are the Hox genes, which define different identities along the anterior-posterior axis. Hox proteins exert their function by interaction wit...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01939
更新日期:2014-03-18 00:00:00
abstract::A mathematical model shows how the shape of early multicellular organisms may have helped cells evolve specialized roles. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.63328
更新日期:2020-11-03 00:00:00
abstract::Manipulating gene function cell type-specifically is a common experimental goal in Drosophila research and has been central to studies of neural development, circuit computation, and behavior. However, current cell type-specific gene disruption techniques in flies often reduce gene activity incompletely or rely on cel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.22279
更新日期:2017-02-17 00:00:00
abstract::Hawaiian isolates of the nematode species Caenorhabditis elegans have long been known to harbor genetic diversity greater than the rest of the worldwide population, but this observation was supported by only a small number of wild strains. To better characterize the niche and genetic diversity of Hawaiian C. elegans a...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50465
更新日期:2019-12-03 00:00:00
abstract::The new open-access journal eLife has launched, making its first content available in PubMed Central. In addition to publishing science of the highest quality, the journal aims to improve both the peer-review process and the presentation of new research results. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00270
更新日期:2012-10-15 00:00:00
abstract::The orbitofrontal cortex (OFC) is critical for updating reward-directed behaviours flexibly when outcomes are devalued or when task contingencies are reversed. Failure to update behaviour in outcome devaluation and reversal learning procedures are considered canonical deficits following OFC lesions in non-human primat...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.37357
更新日期:2018-07-25 00:00:00
abstract::Hair follicle (HF) development is orchestrated by coordinated signals from adjacent epithelial and mesenchymal cells. In humans this process only occurs during embryogenesis and viable strategies to induce new HFs in adult skin are lacking. Here, we reveal that activation of Hedgehog (Hh) signaling in adjacent epithel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46756
更新日期:2020-03-17 00:00:00
abstract::Protein aggregation appears to originate from partially unfolded conformations that are sampled through stochastic fluctuations of the native protein. It has been a challenge to characterize these fluctuations, under native like conditions. Here, the conformational dynamics of the full-length (23-231) mouse prion prot...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44766
更新日期:2019-04-26 00:00:00
abstract::Although the wiring of the cerebellar cortex appears to be uniform, the neurons in this region of the brain behave more differently from each other than previously thought. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.03285
更新日期:2014-06-10 00:00:00
abstract::Layer (L) 2/3 pyramidal neurons in the primary somatosensory cortex (S1) are sparsely active, spontaneously and during sensory stimulation. Long-range inputs from higher areas may gate L2/3 activity. We investigated their in vivo impact by expressing channelrhodopsin in three main sources of feedback to rat S1: primar...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44158
更新日期:2019-02-11 00:00:00
abstract::It is now clear that microglia and macrophages are present in brain tumors, but whether or how they affect initiation and development of tumors is not known. Exploiting the advantages of the zebrafish (Danio rerio) model, we showed that macrophages and microglia respond immediately upon oncogene activation in the brai...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.31918
更新日期:2018-02-21 00:00:00
abstract::During development, tissue repair, and tumor growth, most blood vessel networks are generated through angiogenesis. Vascular endothelial growth factor (VEGF) is a key regulator of this process and currently both VEGF and its receptors, VEGFR1, VEGFR2, and Neuropilin1 (NRP1), are targeted in therapeutic strategies for ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03720
更新日期:2014-09-22 00:00:00
abstract::TCF/LEF factors are ancient context-dependent enhancer-binding proteins that are activated by β-catenin following Wnt signaling. They control embryonic development and adult stem cell compartments, and their dysregulation often causes cancer. β-catenin-dependent transcription relies on the NPF motif of Pygo proteins. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.09073
更新日期:2015-08-27 00:00:00
abstract::Bone strength is determined by its dense cortical shell, generated by unknown mechanisms. Here we use the Dmp1Cre:Socs3f/f mouse, with delayed cortical bone consolidation, to characterise cortical maturation and identify control signals. We show that cortical maturation requires a reduction in cortical porosity, and a...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56666
更新日期:2020-05-27 00:00:00
abstract::Glucocorticoid receptor (GR) is crucial for signaling mediated by stress-induced high levels of glucocorticoids. The lateral nucleus of the amygdala (LA) is a key structure underlying auditory-cued fear conditioning. Here, we demonstrate that genetic disruption of GR in the LA (LAGRKO) resulted in an auditory-cued fea...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.34135
更新日期:2018-06-26 00:00:00
abstract::Nucleolar protein interacting with the FHA domain of pKi-67 (NIFK) is a Ki-67-interacting protein. However, its precise function in cancer remains largely uninvestigated. Here we show the clinical significance and metastatic mechanism of NIFK in lung cancer. NIFK expression is clinically associated with poor prognosis...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11288
更新日期:2016-03-17 00:00:00