Abstract:
:AMPARs control fast synaptic communication between neurons and their function relies on auxiliary subunits, which importantly modulate channel properties. Although it has been suggested that AMPARs can bind to TARPs with variable stoichiometry, little is known about the effect that this stoichiometry exerts on certain AMPAR properties. Here we have found that AMPARs show a clear stoichiometry-dependent modulation by the prototypical TARP γ2 although the receptor still needs to be fully saturated with γ2 to show some typical TARP-induced characteristics (i.e. an increase in channel conductance). We also uncovered important differences in the stoichiometric modulation between calcium-permeable and calcium-impermeable AMPARs. Moreover, in heteromeric AMPARs, γ2 positioning in the complex is important to exert certain TARP-dependent features. Finally, by comparing data from recombinant receptors with endogenous AMPAR currents from mouse cerebellar granule cells, we have determined a likely presence of two γ2 molecules at somatic receptors in this cell type.
journal_name
Elifejournal_title
eLifeauthors
Miguez-Cabello F,Sánchez-Fernández N,Yefimenko N,Gasull X,Gratacòs-Batlle E,Soto Ddoi
10.7554/eLife.53946subject
Has Abstractpub_date
2020-05-26 00:00:00issn
2050-084Xpii
53946journal_volume
9pub_type
杂志文章相关文献
eLife文献大全abstract::Pregnancy complications are poorly represented in the archeological record, despite their importance in contemporary and ancient societies. While excavating a Byzantine cemetery in Troy, we discovered calcified abscesses among a woman's remains. Scanning electron microscopy of the tissue revealed 'ghost cells', result...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20983
更新日期:2017-01-10 00:00:00
abstract::Using a clever combination of genetic and biochemical tools researchers have shown that a transcription factor called USF1 has a central role in determining how mutations of the Clock gene manifest themselves in the behaviour of different mouse strains. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.00659
更新日期:2013-04-09 00:00:00
abstract::DNA is a remarkably precise medium for copying and storing biological information. This high fidelity results from the action of hundreds of genes involved in replication, proofreading, and damage repair. Evolutionary theory suggests that in such a system, selection has limited ability to remove genetic variants that ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.24284
更新日期:2017-04-25 00:00:00
abstract::The nematode Caenorhabditis elegans possesses a simple embryonic nervous system with few enough neurons that the growth of each cell could be followed to provide a systems-level view of development. However, studies of single cell development have largely been conducted in fixed or pre-twitching live embryos, because ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10070
更新日期:2015-12-03 00:00:00
abstract::Why does a first pregnancy after age 35 increase the risk of breast cancer, and what can be done to combat this? ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.01926
更新日期:2013-12-31 00:00:00
abstract::The equilibrium between proliferation and quiescence of myogenic progenitor and stem cells is tightly regulated to ensure appropriate skeletal muscle growth and repair. The non-receptor tyrosine phosphatase Ptpn11 (Shp2) is an important transducer of growth factor and cytokine signals. Here we combined complex genetic...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21552
更新日期:2017-05-02 00:00:00
abstract::Mitochondrial dysfunction is associated with activation of the integrated stress response (ISR) but the underlying triggers remain unclear. We systematically combined acute mitochondrial inhibitors with genetic tools for compartment-specific NADH oxidation to trace mechanisms linking different forms of mitochondrial d...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49178
更新日期:2020-05-28 00:00:00
abstract::Understanding the relationships between different properties of data, such as whether a genome or connectome has information about disease status, is increasingly important. While existing approaches can test whether two properties are related, they may require unfeasibly large sample sizes and often are not interpret...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41690
更新日期:2019-01-15 00:00:00
abstract::Proteins can self-organize into spatial patterns via non-linear dynamic interactions on cellular membranes. Modelling and simulations have shown that small GTPases can generate patterns by coupling guanine nucleotide exchange factors (GEF) to effectors, generating a positive feedback of GTPase activation and membrane ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54434
更新日期:2020-06-08 00:00:00
abstract::India comprises much of the persisting global childhood measles mortality. India implemented a mass second-dose measles immunization campaign in 2010. We used interrupted time series and multilevel regression to quantify the campaign's impact on measles mortality using the nationally representative Million Death Study...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43290
更新日期:2019-03-05 00:00:00
abstract::Macrophage activation/polarization to distinct functional states is critically supported by metabolic shifts. How polarizing signals coordinate metabolic and functional reprogramming, and the potential implications for control of macrophage activation, remains poorly understood. Here we show that IL-4 signaling co-opt...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11612
更新日期:2016-02-19 00:00:00
abstract::The basolateral amygdala (BLA) is critical for associating initially neutral cues with appetitive and aversive stimuli and receives dense neuromodulatory acetylcholine (ACh) projections. We measured BLA ACh signaling and activity of neurons expressing CaMKIIα (a marker for glutamatergic principal cells) in mice during...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.57335
更新日期:2020-09-18 00:00:00
abstract::Animals can effortlessly adapt their behavior by generalizing from past aversive experiences, allowing to avoid harm in novel situations. We studied how visual information was sampled by eye-movements during this process called fear generalization, using faces organized along a circular two-dimensional perceptual cont...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44111
更新日期:2019-10-22 00:00:00
abstract::Transposable elements (TEs), the movement of which can damage the genome, are epigenetically silenced in eukaryotes. Intriguingly, TEs are activated in the sperm companion cell - vegetative cell (VC) - of the flowering plant Arabidopsis thaliana. However, the extent and mechanism of this activation are unknown. Here w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.42530
更新日期:2019-05-28 00:00:00
abstract::The straight-tusked elephants Palaeoloxodon spp. were widespread across Eurasia during the Pleistocene. Phylogenetic reconstructions using morphological traits have grouped them with Asian elephants (Elephas maximus), and many paleontologists place Palaeoloxodon within Elephas. Here, we report the recovery of full mit...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25413
更新日期:2017-06-06 00:00:00
abstract::Sex-specific synaptic connectivity is beginning to emerge as a remarkable, but little explored feature of animal brains. We describe here a novel mechanism that promotes sexually dimorphic neuronal function and synaptic connectivity in the nervous system of the nematode Caenorhabditis elegans. We demonstrate that a ph...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59614
更新日期:2020-10-06 00:00:00
abstract::Recent work draws attention to community-community encounters ('coalescence') as likely an important factor shaping natural ecosystems. This work builds on MacArthur's classic model of competitive coexistence to investigate such community-level competition in a minimal theoretical setting. It is shown that the ability...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15747
更新日期:2016-06-16 00:00:00
abstract::Appropriate generalization of learned responses to new situations is vital for adaptive behavior. We provide a circuit-level account of generalization in the electrosensory lobe (ELL) of weakly electric mormyrid fish. Much is already known in this system about a form of learning in which motor corollary discharge sign...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44032
更新日期:2019-03-14 00:00:00
abstract::Exhaustion of stem cells is a hallmark of aging. In the Drosophila testis, dedifferentiated germline stem cells (GSCs) derived from spermatogonia increase during lifespan, leading to the model that dedifferentiation counteracts the decline of GSCs in aged males. To test this, we blocked dedifferentiation by mis-expres...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.36095
更新日期:2018-07-09 00:00:00
abstract::During embryogenesis, chromatin accessibility profiles control lineage-specific gene expression by modulating transcription, thus impacting multipotent progenitor states and subsequent fate choices. Subsets of cardiac and pharyngeal/head muscles share a common origin in the cardiopharyngeal mesoderm, but the chromatin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49921
更新日期:2019-11-20 00:00:00
abstract::Synaptic adhesion molecules regulate synapse development and function. However, whether and how presynaptic adhesion molecules regulate postsynaptic NMDAR function remains largely unclear. Presynaptic LAR family receptor tyrosine phosphatases (LAR-RPTPs) regulate synapse development through mechanisms that include tra...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54224
更新日期:2020-03-06 00:00:00
abstract::Mature microRNAs (miRNAs) are processed from primary transcripts (pri-miRNAs), and their expression is controlled at transcriptional and post-transcriptional levels. However, how regulation at multiple levels achieves precise control remains elusive. Using published and new datasets, we profile a time course of mature...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.38389
更新日期:2018-07-19 00:00:00
abstract::Nucleolar protein interacting with the FHA domain of pKi-67 (NIFK) is a Ki-67-interacting protein. However, its precise function in cancer remains largely uninvestigated. Here we show the clinical significance and metastatic mechanism of NIFK in lung cancer. NIFK expression is clinically associated with poor prognosis...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11288
更新日期:2016-03-17 00:00:00
abstract::R-loops are features of chromatin consisting of a strand of DNA hybridized to RNA, as well as the expelled complementary DNA strand. R-loops are enriched at promoters where they have recently been shown to have important roles in modifying gene expression. However, the location of promoter-associated R-loops and the g...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.28306
更新日期:2017-10-26 00:00:00
abstract::Sudden changes in the level of a coenzyme called NADPH might be the underlying cause of aging in cells. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.00515
更新日期:2013-02-05 00:00:00
abstract::Developing neurons of the peripheral nervous system reach their targets via cues that support directional growth, a process known as axon guidance. In investigating how sympathetic axons reach the heart in mice, we discovered that a combination of guidance cues are employed in sequence to refine axon outgrowth, a proc...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.42528
更新日期:2019-02-08 00:00:00
abstract::The Deleted in Colorectal Carcinoma (Dcc) receptor plays a critical role in optic nerve development. Whilst Dcc is expressed postnatally in the eye, its function remains unknown as Dcc knockouts die at birth. To circumvent this drawback, we generated an eye-specific Dcc mutant. To study the organization of the retina ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51275
更新日期:2020-02-25 00:00:00
abstract::Two mammalian genes, Kcnt1 and Kcnt2, encode pore-forming subunits of Na(+)-dependent K(+) (KNa) channels. Progress in understanding KNa channels has been hampered by the absence of specific tools and methods for rigorous KNa identification in native cells. Here, we report the genetic disruption of both Kcnt1 and Kcnt...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10013
更新日期:2015-11-11 00:00:00
abstract:: ...
journal_title:eLife
pub_type: 杂志文章,已发布勘误
doi:10.7554/eLife.60401
更新日期:2020-06-30 00:00:00
abstract::During development, tissue repair, and tumor growth, most blood vessel networks are generated through angiogenesis. Vascular endothelial growth factor (VEGF) is a key regulator of this process and currently both VEGF and its receptors, VEGFR1, VEGFR2, and Neuropilin1 (NRP1), are targeted in therapeutic strategies for ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03720
更新日期:2014-09-22 00:00:00