Deprivation-related and use-dependent plasticity go hand in hand.

abstract：:Macrophages are highly plastic cells with critical roles in immunity, cancer, and tissue homeostasis, but how these distinct cellular fates are triggered by environmental cues is poorly understood. To uncover how primary murine macrophages respond to bacterial pathogens, we globally assessed changes in post-translatio...

journal_title：eLife

authors： Budzik JM,Swaney DL,Jimenez-Morales D,Johnson JR,Garelis NE,Repasy T,Roberts AW,Popov LM,Parry TJ,Pratt D,Ideker T,Krogan NJ,Cox JS

更新日期：2020-01-17 00:00:00

abstract：:Certain neuron types fire spontaneously at high rates, an ability that is crucial for their function in brain circuits. The spontaneously active GABAergic neurons of the substantia nigra pars reticulata (SNr), a major output of the basal ganglia, provide tonic inhibition of downstream brain areas. A depolarizing 'leak...

journal_title：eLife

authors： Lutas A,Lahmann C,Soumillon M,Yellen G

更新日期：2016-05-13 00:00:00

abstract：:Individuals vary in their responses to stroke and trauma, hampering predictions of outcomes. One reason might be that neural circuits contain hidden variability that becomes relevant only when those individuals are challenged by injury. We found that in the mollusc, Tritonia diomedea, subtle differences between animal...

journal_title：eLife

authors： Sakurai A,Tamvacakis AN,Katz PS

更新日期：2014-06-11 00:00:00

abstract：:Although individuals of many species inexorably age, a number of observations established that the rate of aging is modulated in response to a variety of mild stresses. Here, we investigated how heat stress promotes longevity in yeast. We show that upon growth at higher temperature, yeast cells relax the retention of ...

journal_title：eLife

authors： Baldi S,Bolognesi A,Meinema AC,Barral Y

更新日期：2017-12-28 00:00:00

abstract：:mTOR inhibition is beneficial in neurodegenerative disease models and its effects are often attributable to the modulation of autophagy and anti-apoptosis. Here, we report a neglected but important bioenergetic effect of mTOR inhibition in neurons. mTOR inhibition by rapamycin significantly preserves neuronal ATP leve...

journal_title：eLife

authors： Zheng X,Boyer L,Jin M,Kim Y,Fan W,Bardy C,Berggren T,Evans RM,Gage FH,Hunter T

更新日期：2016-03-23 00:00:00

abstract：:Developing neurons of the peripheral nervous system reach their targets via cues that support directional growth, a process known as axon guidance. In investigating how sympathetic axons reach the heart in mice, we discovered that a combination of guidance cues are employed in sequence to refine axon outgrowth, a proc...

journal_title：eLife

authors： Poltavski DM,Colombier P,Hu J,Duron A,Black BL,Makita T

更新日期：2019-02-08 00:00:00

abstract：:The final identity of a differentiated neuron is determined by multiple signaling events, including activity dependent calcium transients. Non-canonical Frizzled2 (Fz2) signaling generates calcium transients that determine neuronal polarity, neuronal migration, and synapse assembly in the developing vertebrate brain. ...

journal_title：eLife

authors： Agrawal T,Hasan G

更新日期：2015-05-08 00:00:00

abstract：:Hawaiian isolates of the nematode species Caenorhabditis elegans have long been known to harbor genetic diversity greater than the rest of the worldwide population, but this observation was supported by only a small number of wild strains. To better characterize the niche and genetic diversity of Hawaiian C. elegans a...

journal_title：eLife

authors： Crombie TA,Zdraljevic S,Cook DE,Tanny RE,Brady SC,Wang Y,Evans KS,Hahnel S,Lee D,Rodriguez BC,Zhang G,van der Zwagg J,Kiontke K,Andersen EC

更新日期：2019-12-03 00:00:00

abstract：:The transcription factor ASCIZ (ATMIN, ZNF822) has an unusually high number of recognition motifs for the product of its main target gene, the hub protein LC8 (DYNLL1). Using a combination of biophysical methods, structural analysis by NMR and electron microscopy, and cellular transcription assays, we developed a mode...

journal_title：eLife

authors： Clark S,Myers JB,King A,Fiala R,Novacek J,Pearce G,Heierhorst J,Reichow SL,Barbar EJ

更新日期：2018-05-01 00:00:00

abstract：:Microtubules are nucleated from specific locations at precise times in the cell cycle. However, the factors that constitute these microtubule nucleation pathways and their mode of action still need to be identified. Using purified Xenopus laevis proteins we biochemically reconstitute branching microtubule nucleation, ...

journal_title：eLife

authors： Alfaro-Aco R,Thawani A,Petry S

更新日期：2020-01-14 00:00:00

abstract：:Embryonic polarity of invertebrates, amphibians and fish is specified largely by maternal determinants, which fixes cell fates early in development. In contrast, amniote embryos remain plastic and can form multiple individuals until gastrulation. How is their polarity determined? In the chick embryo, the earliest know...

journal_title：eLife

authors： Torlopp A,Khan MA,Oliveira NM,Lekk I,Soto-Jiménez LM,Sosinsky A,Stern CD

更新日期：2014-12-11 00:00:00

abstract：:TMEM16A is a ligand-gated anion channel that is activated by intracellular Ca2+. This channel comprises two independent pores and closely apposed Ca2+ binding sites that are contained within each subunit of a homodimeric protein. Previously we characterized the influence of positively charged pore-lining residues on a...

journal_title：eLife

authors： Lam AK,Dutzler R

更新日期：2018-10-12 00:00:00

abstract：:In most animals, the brain controls the body via a set of descending neurons (DNs) that traverse the neck. DN activity activates, maintains or modulates locomotion and other behaviors. Individual DNs have been well-studied in species from insects to primates, but little is known about overall connectivity patterns acr...

journal_title：eLife

authors： Namiki S,Dickinson MH,Wong AM,Korff W,Card GM

更新日期：2018-06-26 00:00:00

abstract：:High-altitude environments require that animals meet the metabolic O2 demands for locomotion and thermogenesis in O2-thin air, but the degree to which convergent metabolic changes have arisen across independent high-altitude lineages or the speed at which such changes arise is unclear. We examined seven high-altitude ...

journal_title：eLife

authors： Dawson NJ,Alza L,Nandal G,Scott GR,McCracken KG

更新日期：2020-07-30 00:00:00

abstract：:Pervasive expression of circular RNA is a recently discovered feature of eukaryotic gene expression programs, yet its function remains largely unknown. The presumed biogenesis of these RNAs involves a non-canonical 'backsplicing' event. Recent studies in mammalian cell culture posit that backsplicing is facilitated by...

journal_title：eLife

authors： Barrett SP,Wang PL,Salzman J

更新日期：2015-06-09 00:00:00

abstract：:Exhaustion of stem cells is a hallmark of aging. In the Drosophila testis, dedifferentiated germline stem cells (GSCs) derived from spermatogonia increase during lifespan, leading to the model that dedifferentiation counteracts the decline of GSCs in aged males. To test this, we blocked dedifferentiation by mis-expres...

journal_title：eLife

authors： Herrera SC,Bach EA

更新日期：2018-07-09 00:00:00

abstract：:Protein aggregation appears to originate from partially unfolded conformations that are sampled through stochastic fluctuations of the native protein. It has been a challenge to characterize these fluctuations, under native like conditions. Here, the conformational dynamics of the full-length (23-231) mouse prion prot...

journal_title：eLife

authors： Goluguri RR,Sen S,Udgaonkar J

更新日期：2019-04-26 00:00:00

abstract：:Rapid and flexible interpretation of conflicting sensory inputs in the context of current goals is a critical component of cognitive control that is orchestrated by frontal cortex. The relative roles of distinct subregions within frontal cortex are poorly understood. To examine the dynamics underlying cognitive contro...

journal_title：eLife

authors： Tang H,Yu HY,Chou CC,Crone NE,Madsen JR,Anderson WS,Kreiman G

更新日期：2016-02-18 00:00:00

abstract：:The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...

journal_title：eLife

authors： Lischinsky JE,Sokolowski K,Li P,Esumi S,Kamal Y,Goodrich M,Oboti L,Hammond TR,Krishnamoorthy M,Feldman D,Huntsman M,Liu J,Corbin JG

更新日期：2017-03-13 00:00:00

abstract：:The Maternal Embryonic Leucine Zipper Kinase (MELK) has been identified as a promising therapeutic target in multiple cancer types. MELK over-expression is associated with aggressive disease, and MELK has been implicated in numerous cancer-related processes, including chemotherapy resistance, stem cell renewal, and tu...

journal_title：eLife

authors： Giuliano CJ,Lin A,Smith JC,Palladino AC,Sheltzer JM

更新日期：2018-02-08 00:00:00

abstract：:Gustatory pheromones play an essential role in shaping the behavior of many organisms. However, little is known about the processing of taste pheromones in higher order brain centers. Here, we describe a male-specific gustatory circuit in Drosophila that underlies the detection of the anti-aphrodisiac pheromone (3R,11...

journal_title：eLife

authors： Shankar S,Chua JY,Tan KJ,Calvert ME,Weng R,Ng WC,Mori K,Yew JY

更新日期：2015-06-17 00:00:00

abstract：:Plasma cells (PCs) are essential for protection from infection, and at the origin of incurable cancers. Current studies do not circumvent the limitations of removing PCs from their microenvironment and confound formation and maintenance. Also, the investigation of PC population dynamics has mostly relied on nucleotide...

journal_title：eLife

authors： Xu AQ,Barbosa RR,Calado DP

更新日期：2020-11-02 00:00:00

abstract：:Contrary to dogma, evolutionarily young and dynamic genes can encode essential functions. We find that evolutionarily dynamic ZAD-ZNF genes, which encode the most abundant class of insect transcription factors, are more likely to encode essential functions in Drosophila melanogaster than ancient, conserved ZAD-ZNF gen...

journal_title：eLife

authors： Kasinathan B,Colmenares SU 3rd,McConnell H,Young JM,Karpen GH,Malik HS

更新日期：2020-11-10 00:00:00

abstract：:Ion channel models are the building blocks of computational neuron models. Their biological fidelity is therefore crucial for the interpretation of simulations. However, the number of published models, and the lack of standardization, make the comparison of ion channel models with one another and with experimental dat...

journal_title：eLife

authors： Podlaski WF,Seeholzer A,Groschner LN,Miesenböck G,Ranjan R,Vogels TP

更新日期：2017-03-06 00:00:00

abstract：:Ten countries have reported pfhrp2/pfhrp3 gene deletions since the first observation of pfhrp2-deleted parasites in 2012. In a previous study (Watson et al., 2017), we characterised the drivers selecting for pfhrp2/3 deletions and mapped the regions in Africa with the greatest selection pressure. In February 2018, the...

journal_title：eLife

authors： Watson OJ,Verity R,Ghani AC,Garske T,Cunningham J,Tshefu A,Mwandagalirwa MK,Meshnick SR,Parr JB,Slater HC

更新日期：2019-05-02 00:00:00

abstract：:Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric compon...

journal_title：eLife

authors： Spletter ML,Barz C,Yeroslaviz A,Zhang X,Lemke SB,Bonnard A,Brunner E,Cardone G,Basler K,Habermann BH,Schnorrer F

更新日期：2018-05-30 00:00:00

abstract：:The contribution of insular cortex to speech production remains unclear and controversial given diverse findings from functional neuroimaging and lesional data. To create a precise spatiotemporal map of insular activity, we performed a series of experiments: single-word articulations of varying complexity, non-speech ...

journal_title：eLife

authors： Woolnough O,Forseth KJ,Rollo PS,Tandon N

更新日期：2019-12-19 00:00:00

abstract：:Organisms possessing genetic codes with unassigned codons raise the question of how cellular machinery resolves such codons and how this could impact horizontal gene transfer. Here, we use a genomically recoded Escherichia coli to examine how organisms address translation at unassigned UAG codons, which obstruct propa...

journal_title：eLife

authors： Ma NJ,Hemez CF,Barber KW,Rinehart J,Isaacs FJ

更新日期：2018-10-30 00:00:00

abstract：:Temperature-sensitive transient receptor potential (TRP) ion channels are members of the large tetrameric cation channels superfamily but are considered to be uniquely sensitive to heat, which has been presumed to be due to the existence of an unidentified temperature-sensing domain. Here we report that the homologous...

journal_title：eLife

authors： Yang F,Zheng J

更新日期：2014-07-16 00:00:00

abstract：:Cyanobacteria were responsible for the oxygenation of the ancient atmosphere; however, the evolution of this phylum is enigmatic, as relatives have not been characterized. Here we use whole genome reconstruction of human fecal and subsurface aquifer metagenomic samples to obtain complete genomes for members of a new c...

journal_title：eLife

authors： Di Rienzi SC,Sharon I,Wrighton KC,Koren O,Hug LA,Thomas BC,Goodrich JK,Bell JT,Spector TD,Banfield JF,Ley RE

更新日期：2013-10-01 00:00:00