Microsecond sub-domain motions and the folding and misfolding of the mouse prion protein.

abstract：:The ubiquitous presence of inhibitory interneurons in the thalamus of primates contrasts with the sparsity of interneurons reported in mice. Here, we identify a larger than expected complexity and distribution of interneurons across the mouse thalamus, where all thalamic interneurons can be traced back to two developm...

journal_title：eLife

authors： Jager P,Moore G,Calpin P,Durmishi X,Salgarella I,Menage L,Kita Y,Wang Y,Kim DW,Blackshaw S,Schultz SR,Brickley S,Shimogori T,Delogu A

更新日期：2021-02-01 00:00:00

abstract：:Cohesin tethers together regions of DNA, thereby mediating higher order chromatin organization that is critical for sister chromatid cohesion, DNA repair and transcriptional regulation. Cohesin contains a heterodimeric ATP-binding Cassette (ABC) ATPase comprised of Smc1 and Smc3 ATPase active sites. These ATPases are ...

journal_title：eLife

authors： Çamdere G,Guacci V,Stricklin J,Koshland D

更新日期：2015-11-19 00:00:00

abstract：:Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans after silicone oil (S...

journal_title：eLife

authors： Zhang J,Li L,Huang H,Fang F,Webber HC,Zhuang P,Liu L,Dalal R,Tang PH,Mahajan VB,Sun Y,Li S,Zhang M,Goldberg JL,Hu Y

更新日期：2019-05-15 00:00:00

abstract：:TOR complex 1 (TORC1) is an evolutionarily conserved protein kinase complex that promotes cellular macromolecular synthesis and suppresses autophagy. Amino-acid-induced activation of mammalian TORC1 is initiated by its recruitment to the RagA/B-RagC/D GTPase heterodimer, which is anchored to lysosomal membranes throug...

journal_title：eLife

authors： Chia KH,Fukuda T,Sofyantoro F,Matsuda T,Amai T,Shiozaki K

更新日期：2017-12-04 00:00:00

abstract：:Group B Streptococcus (GBS) is the leading cause of invasive bacterial neonatal infections. Late-onset diseases (LOD) occur between 7 and 89 days of life and are largely due to the CC17 GBS hypervirulent clone. We studied the impact of estradiol (E2) and progesterone (P4), which impregnate the fetus during pregnancy, ...

journal_title：eLife

authors： Hays C,Touak G,Bouaboud A,Fouet A,Guignot J,Poyart C,Tazi A

更新日期：2019-11-11 00:00:00

abstract：:Inhibition plays a powerful role in regulating network excitation and plasticity; however, the activity of defined interneuron types during spatial exploration remain poorly understood. Using two-photon calcium imaging, we recorded hippocampal CA1 somatostatin- and parvalbumin-expressing interneurons as mice performed...

journal_title：eLife

authors： Arriaga M,Han EB

更新日期：2019-10-08 00:00:00

abstract：:Experiments in which monkeys have to select or estimate the location of a target are revealing more about the role of the dorsal premotor cortex in decision making. ...

journal_title：eLife

authors： Stuphorn V

更新日期：2016-07-15 00:00:00

abstract：:Sudden changes in the level of a coenzyme called NADPH might be the underlying cause of aging in cells. ...

journal_title：eLife

authors： Barral Y

更新日期：2013-02-05 00:00:00

abstract：:Cell surface reception of Sonic hedgehog (Shh) must ensure that the graded morphogenic signal is interpreted accordingly in neighboring cells to specify tissue patterns during development. Here, we report endocytic sorting signals for the receptor Patched1 (Ptch1), comprising two 'PPXY' motifs, that direct it to degra...

journal_title：eLife

authors： Yue S,Tang LY,Tang Y,Tang Y,Shen QH,Ding J,Chen Y,Zhang Z,Yu TT,Zhang YE,Cheng SY

更新日期：2014-06-12 00:00:00

abstract：:Many marine animals, ranging from corals to fishes, synchronise reproduction to lunar cycles. In the annelid Platynereis dumerilii, this timing is orchestrated by an endogenous monthly (circalunar) clock entrained by moonlight. Whereas daily (circadian) clocks cause extensive transcriptomic and proteomic changes, the ...

journal_title：eLife

authors： Schenk S,Bannister SC,Sedlazeck FJ,Anrather D,Minh BQ,Bileck A,Hartl M,von Haeseler A,Gerner C,Raible F,Tessmar-Raible K

更新日期：2019-02-15 00:00:00

abstract：:An 'interactome' screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interacto...

journal_title：eLife

authors： Li H,Watson A,Olechwier A,Anaya M,Sorooshyari SK,Harnett DP,Lee HP,Vielmetter J,Fares MA,Garcia KC,Özkan E,Labrador JP,Zinn K

更新日期：2017-08-15 00:00:00

abstract：:Mutant KRAS colorectal cancer (CRC) cells release protein-laden exosomes that can alter the tumor microenvironment. To test whether exosomal RNAs also contribute to changes in gene expression in recipient cells, and whether mutant KRAS might regulate the composition of secreted microRNAs (miRNAs), we compared small RN...

journal_title：eLife

authors： Cha DJ,Franklin JL,Dou Y,Liu Q,Higginbotham JN,Demory Beckler M,Weaver AM,Vickers K,Prasad N,Levy S,Zhang B,Coffey RJ,Patton JG

更新日期：2015-07-01 00:00:00

abstract：:Peptidoglycan (PG) is the main component of bacterial cell walls and the target for many antibiotics. PG biosynthesis is tightly coordinated with cell wall growth and turnover, and many of these control activities depend upon PASTA-domain containing eukaryotic-like serine/threonine protein kinases (PASTA-eSTK) that se...

journal_title：eLife

authors： Wamp S,Rutter ZJ,Rismondo J,Jennings CE,Möller L,Lewis RJ,Halbedel S

更新日期：2020-05-29 00:00:00

abstract：:Certain neuron types fire spontaneously at high rates, an ability that is crucial for their function in brain circuits. The spontaneously active GABAergic neurons of the substantia nigra pars reticulata (SNr), a major output of the basal ganglia, provide tonic inhibition of downstream brain areas. A depolarizing 'leak...

journal_title：eLife

authors： Lutas A,Lahmann C,Soumillon M,Yellen G

更新日期：2016-05-13 00:00:00

abstract：:Junín virus (JUNV) is one of five New World mammarenaviruses (NWMs) that causes fatal hemorrhagic disease in humans and is the etiological agent of Argentine hemorrhagic fever (AHF). The pathogenesis underlying AHF is poorly understood; however, a prolonged, elevated interferon-α (IFN-α) response is associated with a ...

journal_title：eLife

authors： Hickerson BT,Sefing EJ,Bailey KW,Van Wettere AJ,Penichet ML,Gowen BB

更新日期：2020-05-26 00:00:00

abstract：:Humans and other animals base important decisions on estimates of number, and intraparietal cortex is thought to provide a crucial substrate of this ability. However, it remains debated whether an independent neuronal processing mechanism underlies this 'number sense', or whether number is instead judged indirectly on...

journal_title：eLife

authors： Castaldi E,Piazza M,Dehaene S,Vignaud A,Eger E

更新日期：2019-07-24 00:00:00

abstract：:Rhabdomyosarcoma (RMS) is a pediatric malignacy of muscle with myogenic regulatory transcription factors MYOD and MYF5 being expressed in this disease. Consensus in the field has been that expression of these factors likely reflects the target cell of transformation rather than being required for continued tumor growt...

journal_title：eLife

authors： Tenente IM,Hayes MN,Ignatius MS,McCarthy K,Yohe M,Sindiri S,Gryder B,Oliveira ML,Ramakrishnan A,Tang Q,Chen EY,Petur Nielsen G,Khan J,Langenau DM

更新日期：2017-01-12 00:00:00

abstract：:The sequential activation of neurons has been observed in various areas of the brain, but in no case is the underlying network structure well understood. Here we examined the circuit anatomy of zebra finch HVC, a cortical region that generates sequences underlying the temporal progression of the song. We combined seri...

journal_title：eLife

authors： Kornfeld J,Benezra SE,Narayanan RT,Svara F,Egger R,Oberlaender M,Denk W,Long MA

更新日期：2017-03-27 00:00:00

abstract：:WW domain tandem-containing proteins such as KIBRA, YAP, and MAGI play critical roles in cell growth and polarity via binding to and positioning target proteins in specific subcellular regions. An immense disparity exists between promiscuity of WW domain-mediated target bindings and specific roles of WW domain protein...

journal_title：eLife

authors： Lin Z,Yang Z,Xie R,Ji Z,Guan K,Zhang M

更新日期：2019-09-05 00:00:00

abstract：:Bacillus subtilis can measure the activity of the enzymes that remodel the cell wall to ensure that the levels of activity are 'just right'. ...

journal_title：eLife

authors： Kim IM,Szurmant H

更新日期：2020-01-24 00:00:00

abstract：:Acquisition of pluripotency is driven largely at the transcriptional level by activators OCT4, SOX2, and NANOG that must in turn cooperate with diverse coactivators to execute stem cell-specific gene expression programs. Using a biochemically defined in vitro transcription system that mediates OCT4/SOX2 and coactivato...

journal_title：eLife

authors： Fong YW,Ho JJ,Inouye C,Tjian R

更新日期：2014-11-19 00:00:00

abstract：:Ageing is characterised by cellular senescence, leading to imbalanced tissue maintenance, cell death and compromised organ function. This is first observed in the thymus, the primary lymphoid organ that generates and selects T cells. However, the molecular and cellular mechanisms underpinning these ageing processes re...

journal_title：eLife

authors： Baran-Gale J,Morgan MD,Maio S,Dhalla F,Calvo-Asensio I,Deadman ME,Handel AE,Maynard A,Chen S,Green F,Sit RV,Neff NF,Darmanis S,Tan W,May AP,Marioni JC,Ponting CP,Holländer GA

更新日期：2020-08-25 00:00:00

abstract：:Mood and anxiety disorders are the most prevalent psychiatric conditions and are exacerbated by stress. Recent studies have suggested cyclooxygenase-2 (COX-2) inhibition could represent a novel treatment approach or augmentation strategy for affective disorders including anxiety disorders and major depression. We show...

journal_title：eLife

authors： Gamble-George JC,Baldi R,Halladay L,Kocharian A,Hartley N,Silva CG,Roberts H,Haymer A,Marnett LJ,Holmes A,Patel S

更新日期：2016-05-10 00:00:00

abstract：:Mammalian HMG-CoA reductase (HMGCR), the rate-limiting enzyme of the cholesterol biosynthetic pathway and the therapeutic target of statins, is post-transcriptionally regulated by sterol-accelerated degradation. Under cholesterol-replete conditions, HMGCR is ubiquitinated and degraded, but the identity of the E3 ubiqu...

journal_title：eLife

authors： Menzies SA,Volkmar N,van den Boomen DJ,Timms RT,Dickson AS,Nathan JA,Lehner PJ

更新日期：2018-12-13 00:00:00

abstract：:A fluorescent imaging technique called fastFISH has been used to track the various steps involved in the transcription of a single DNA molecule. ...

journal_title：eLife

authors： Monnet J,Strick TR

更新日期：2014-01-01 00:00:00

abstract：:Pregnancy complications are poorly represented in the archeological record, despite their importance in contemporary and ancient societies. While excavating a Byzantine cemetery in Troy, we discovered calcified abscesses among a woman's remains. Scanning electron microscopy of the tissue revealed 'ghost cells', result...

journal_title：eLife

authors： Devault AM,Mortimer TD,Kitchen A,Kiesewetter H,Enk JM,Golding GB,Southon J,Kuch M,Duggan AT,Aylward W,Gardner SN,Allen JE,King AM,Wright G,Kuroda M,Kato K,Briggs DE,Fornaciari G,Holmes EC,Poinar HN,Pepperell CS

更新日期：2017-01-10 00:00:00

abstract：:Even when we are at rest, our spinal cords show spontaneous, yet well organised, fluctuations of activity that might reflect sensory and motor networks. ...

journal_title：eLife

authors： Eippert F,Tracey I

更新日期：2014-08-05 00:00:00

abstract：:Entry and extrusion of cations are essential processes in living cells. In alkaliphilic prokaryotes, high external pH activates voltage-gated sodium channels (Nav), which allows Na(+) to enter and be used as substrate for cation/proton antiporters responsible for cytoplasmic pH homeostasis. Here, we describe a new mem...

journal_title：eLife

authors： DeCaen PG,Takahashi Y,Krulwich TA,Ito M,Clapham DE

更新日期：2014-11-11 00:00:00

abstract：:A major puzzle in biology is how mammalian sperm maintain the correct swimming direction during various phases of the sexual reproduction process. Whilst chemotaxis may dominate near the ovum, it is unclear which cues guide spermatozoa on their long journey towards the egg. Hypothesized mechanisms range from peristalt...

journal_title：eLife

authors： Kantsler V,Dunkel J,Blayney M,Goldstein RE

更新日期：2014-05-27 00:00:00

abstract：:Termination of messenger RNA translation in Bacteria and Archaea is initiated by release factors (RFs) 1 or 2 recognizing a stop codon in the ribosomal A site and releasing the peptide from the P-site transfer RNA. After release, RF-dissociation is facilitated by the G-protein RF3. Structures of ribosomal complexes wi...

journal_title：eLife

authors： Pallesen J,Hashem Y,Korkmaz G,Koripella RK,Huang C,Ehrenberg M,Sanyal S,Frank J

更新日期：2013-06-04 00:00:00