A transcriptomics resource reveals a transcriptional transition during ordered sarcomere morphogenesis in flight muscle.

abstract：:Although activation/deactivation of specific brain regions has been shown to be predictive of successful memory encoding, the relationship between time-varying large-scale brain networks and fluctuations of memory encoding performance remains unclear. Here, we investigated time-varying functional connectivity patterns...

journal_title：eLife

authors： Keerativittayayut R,Aoki R,Sarabi MT,Jimura K,Nakahara K

更新日期：2018-06-18 00:00:00

abstract：:Regulation of food intake is fundamental to energy homeostasis in animals. The contribution of non-nutritive and metabolic signals in regulating feeding is unclear. Here we show that enteric neurons play a major role in regulating feeding through specialized mechanosensory ion channels in Drosophila. Modulating activi...

journal_title：eLife

authors： Olds WH,Xu T

更新日期：2014-10-06 00:00:00

abstract：:AKT is implicated in neurological disorders. AKT has three isoforms, AKT1/AKT2/AKT3, with brain cell type-specific expression that may differentially influence behavior. Therefore, we examined single Akt isoform, conditional brain-specific Akt1, and double Akt1/3 mutant mice in behaviors relevant to neuropsychiatric d...

journal_title：eLife

authors： Wong H,Levenga J,LaPlante L,Keller B,Cooper-Sansone A,Borski C,Milstead R,Ehringer M,Hoeffer C

更新日期：2020-12-16 00:00:00

abstract：:Over 80% of multiple-tested siRNAs and shRNAs targeting CD95 or CD95 ligand (CD95L) induce a form of cell death characterized by simultaneous activation of multiple cell death pathways preferentially killing transformed and cancer stem cells. We now show these si/shRNAs kill cancer cells through canonical RNAi by targ...

journal_title：eLife

authors： Putzbach W,Gao QQ,Patel M,van Dongen S,Haluck-Kangas A,Sarshad AA,Bartom ET,Kim KA,Scholtens DM,Hafner M,Zhao JC,Murmann AE,Peter ME

更新日期：2017-10-24 00:00:00

abstract：:Chemosensory neurons extract information about chemical cues from the environment. How is the activity in these sensory neurons transformed into behavior? Using Caenorhabditis elegans, we map a novel sensory neuron circuit motif that encodes odor concentration. Primary neurons, AWC(ON) and AWA, directly detect the foo...

journal_title：eLife

authors： Leinwand SG,Yang CJ,Bazopoulou D,Chronis N,Srinivasan J,Chalasani SH

更新日期：2015-09-22 00:00:00

abstract：:The expression of E-Cadherin, a protein best known for its role in cell adhesion, regulates the onset of embryonic differentiation. ...

journal_title：eLife

authors： Sancho M,Rodríguez TA

更新日期：2013-12-17 00:00:00

abstract：:Human patients carrying PAPP-A2 inactivating mutations have low bone mineral density. The underlying mechanisms for this reduced calcification are poorly understood. Using a zebrafish model, we report that Papp-aa regulates bone calcification by promoting Ca2+-transporting epithelial cell (ionocyte) quiescence-prolife...

journal_title：eLife

authors： Liu C,Li S,Noer PR,Kjaer-Sorensen K,Juhl AK,Goldstein A,Ke C,Oxvig C,Duan C

更新日期：2020-04-16 00:00:00

abstract：:Post-transcriptional control by small regulatory RNA (sRNA) is critical for rapid adaptive processes. sRNAs can directly modulate mRNA degradation in Proteobacteria without interfering with translation. However, Firmicutes have a fundamentally different set of ribonucleases for mRNA degradation and whether sRNAs can r...

journal_title：eLife

authors： Durand S,Braun F,Helfer AC,Romby P,Condon C

更新日期：2017-04-24 00:00:00

abstract：:Loss of polarity correlates with progression of epithelial cancers, but how plasma membrane misorganization drives oncogenic transcriptional events remains unclear. The polarity regulators of the Drosophila Scribble (Scrib) module are potent tumor suppressors and provide a model for mechanistic investigation. RNA prof...

journal_title：eLife

authors： Bunker BD,Nellimoottil TT,Boileau RM,Classen AK,Bilder D

更新日期：2015-02-26 00:00:00

abstract：:Interaction between disease-microbiome associations and ageing has not been explored in detail. Here, using age/region-matched sub-sets, we analysed the gut microbiome differences across five major diseases in a multi-cohort dataset constituting more than 2500 individuals from 20 to 89 years old. We show that disease-...

journal_title：eLife

authors： Ghosh TS,Das M,Jeffery IB,O'Toole PW

更新日期：2020-03-11 00:00:00

abstract：:Anterograde transport of late endosomes or lysosomes (LE/Lys) is crucial for proper axon growth. However, the role of energetic nutrients has been poorly explored. Malonyl-CoA is a precursor of fatty acids, and its intracellular levels highly fluctuate depending on glucose availability or the energy sensor AMP-activat...

journal_title：eLife

authors： Palomo-Guerrero M,Fadó R,Casas M,Pérez-Montero M,Baena M,Helmer PO,Domínguez JL,Roig A,Serra D,Hayen H,Stenmark H,Raiborg C,Casals N

更新日期：2019-12-23 00:00:00

abstract：:The IFNλ family of interferons controls the spread of viruses in the upper respiratory tract and transmission between mice. ...

journal_title：eLife

authors： Wack A

更新日期：2018-05-24 00:00:00

abstract：:Postmitotic tissues are incapable of replacing damaged cells through proliferation, but need to rely on buffering mechanisms to prevent tissue disintegration. By constitutively activating the Ras/MAPK-pathway via RasV12-overexpression in the postmitotic salivary glands of Drosophila larvae, we overrode the glands adap...

journal_title：eLife

authors： Krautz R,Khalili D,Theopold U

更新日期：2020-12-30 00:00:00

abstract：:Engineered molecules based on human cytokines have potential uses in research and medicine. ...

journal_title：eLife

authors： Villarino A,O'Shea JJ

更新日期：2017-05-12 00:00:00

abstract：:The organization and biophysical properties of the cytosol implicitly govern molecular interactions within cells. However, little is known about mechanisms by which cells regulate cytosolic properties and intracellular diffusion rates. Here, we demonstrate that the intracellular environment of budding yeast undertakes...

journal_title：eLife

authors： Joyner RP,Tang JH,Helenius J,Dultz E,Brune C,Holt LJ,Huet S,Müller DJ,Weis K

更新日期：2016-03-22 00:00:00

abstract：:Two recently discovered transcription factors stop cells from dividing when plants face extreme heat and DNA damage. ...

journal_title：eLife

authors： Eekhout T,De Veylder L

更新日期：2019-04-09 00:00:00

abstract：:The multi-domain splicing factor RBM5 regulates the balance between antagonistic isoforms of the apoptosis-control genes FAS/CD95, Caspase-2 and AID. An OCRE (OCtamer REpeat of aromatic residues) domain found in RBM5 is important for alternative splicing regulation and mediates interactions with components of the U4/U...

journal_title：eLife

authors： Mourão A,Bonnal S,Soni K,Warner L,Bordonné R,Valcárcel J,Sattler M

更新日期：2016-11-29 00:00:00

abstract：:CRISPR-based homing gene drives have sparked both enthusiasm and deep concerns due to their potential for genetically altering entire species. This raises the question about our ability to prevent the unintended spread of such drives from the laboratory into a natural population. Here, we experimentally demonstrate th...

journal_title：eLife

authors： Champer J,Chung J,Lee YL,Liu C,Yang E,Wen Z,Clark AG,Messer PW

更新日期：2019-01-22 00:00:00

abstract：:The canonical action of the p85α regulatory subunit of phosphatidylinositol 3-kinase (PI3K) is to associate with the p110α catalytic subunit to allow stimuli-dependent activation of the PI3K pathway. We elucidate a p110α-independent role of homodimerized p85α in the positive regulation of PTEN stability and activity. ...

journal_title：eLife

authors： Cheung LW,Walkiewicz KW,Besong TM,Guo H,Hawke DH,Arold ST,Mills GB

更新日期：2015-07-29 00:00:00

abstract：:Using a clever combination of genetic and biochemical tools researchers have shown that a transcription factor called USF1 has a central role in determining how mutations of the Clock gene manifest themselves in the behaviour of different mouse strains. ...

journal_title：eLife

authors： Demarque M,Schibler U

更新日期：2013-04-09 00:00:00

abstract：:V-ATPase is an energy converting enzyme, coupling ATP hydrolysis/synthesis in the hydrophilic V1 domain, with proton flow through the Vo membrane domain, via rotation of the central rotor complex relative to the surrounding stator apparatus. Upon dissociation from the V1 domain, the Vo domain of the eukaryotic V-ATPas...

journal_title：eLife

authors： Kishikawa JI,Nakanishi A,Furuta A,Kato T,Namba K,Tamakoshi M,Mitsuoka K,Yokoyama K

更新日期：2020-07-08 00:00:00

abstract：:T follicular helper cells (Tfh) are critical for the longevity and quality of antibody-mediated protection against infection. Yet few signaling pathways have been identified to be unique solely to Tfh development. ROQUIN is a post-transcriptional repressor of T cells, acting through its ROQ domain to destabilize mRNA ...

journal_title：eLife

authors： Ramiscal RR,Parish IA,Lee-Young RS,Babon JJ,Blagih J,Pratama A,Martin J,Hawley N,Cappello JY,Nieto PF,Ellyard JI,Kershaw NJ,Sweet RA,Goodnow CC,Jones RG,Febbraio MA,Vinuesa CG,Athanasopoulos V

更新日期：2015-10-23 00:00:00

abstract：:Plasma Membrane is the primary structure for adjusting to ever changing conditions. PM sub-compartmentalization in domains is thought to orchestrate signaling. Yet, mechanisms governing membrane organization are mostly uncharacterized. The plant-specific REMORINs are proteins regulating hormonal crosstalk and host inv...

journal_title：eLife

authors： Gronnier J,Crowet JM,Habenstein B,Nasir MN,Bayle V,Hosy E,Platre MP,Gouguet P,Raffaele S,Martinez D,Grelard A,Loquet A,Simon-Plas F,Gerbeau-Pissot P,Der C,Bayer EM,Jaillais Y,Deleu M,Germain V,Lins L,Mongrand S

更新日期：2017-07-31 00:00:00

abstract：:Conventional Ly6Chi monocytes have developmental plasticity for a spectrum of differentiated phagocytes. Here we show, using conditional deletion strategies in a mouse model of Toll-like receptor (TLR) 7-induced inflammation, that the spectrum of developmental cell fates of Ly6Chi monocytes, and the resultant inflamma...

journal_title：eLife

authors： Gamrekelashvili J,Kapanadze T,Sablotny S,Ratiu C,Dastagir K,Lochner M,Karbach S,Wenzel P,Sitnow A,Fleig S,Sparwasser T,Kalinke U,Holzmann B,Haller H,Limbourg FP

更新日期：2020-07-29 00:00:00

abstract：:Hox genes are essential regulators of embryonic development. Their step-wise transcriptional activation follows their genomic topology and the various states of activation are subsequently memorized into domains of progressively overlapping gene products. We have analyzed the 3D chromatin organization of Hox clusters ...

journal_title：eLife

authors： Noordermeer D,Leleu M,Schorderet P,Joye E,Chabaud F,Duboule D

更新日期：2014-04-29 00:00:00

abstract：:Humans employ different strategies when making decisions. Previous research has reported reduced reliance on model-based strategies with aging, but it remains unclear whether this is due to cognitive or motivational factors. Moreover, it is not clear how aging affects the metacontrol of decision making, that is the dy...

journal_title：eLife

authors： Bolenz F,Kool W,Reiter AM,Eppinger B

更新日期：2019-08-09 00:00:00

abstract：:A hallmark of CRISPR-Cas immunity systems is the CRISPR array, a genomic locus consisting of short, repeated sequences ('repeats') interspersed with short, variable sequences ('spacers'). CRISPR arrays are transcribed and processed into individual CRISPR RNAs that each include a single spacer, and direct Cas proteins ...

journal_title：eLife

authors： Stringer AM,Baniulyte G,Lasek-Nesselquist E,Seed KD,Wade JT

更新日期：2020-10-30 00:00:00

abstract：:Engaging with vocal sounds is critical for children's social-emotional learning, and children with autism spectrum disorder (ASD) often 'tune out' voices in their environment. Little is known regarding the neurobiological basis of voice processing and its link to social impairments in ASD. Here, we perform the first c...

journal_title：eLife

authors： Abrams DA,Padmanabhan A,Chen T,Odriozola P,Baker AE,Kochalka J,Phillips JM,Menon V

更新日期：2019-02-26 00:00:00

abstract：:During outbreaks of high-consequence pathogens, airport screening programs have been deployed to curtail geographic spread of infection. The effectiveness of screening depends on several factors, including pathogen natural history and epidemiology, human behavior, and characteristics of the source epidemic. We develop...

journal_title：eLife

authors： Gostic KM,Kucharski AJ,Lloyd-Smith JO

更新日期：2015-02-19 00:00:00

abstract：:Mycobacterium tuberculosis (Mtb) expresses a broad-spectrum β-lactamase (BlaC) that mediates resistance to one of the highly effective antibacterials, β-lactams. Nonetheless, β-lactams showed mycobactericidal activity in combination with β-lactamase inhibitor, clavulanate (Clav). However, the mechanistic aspects of ho...

journal_title：eLife

authors： Mishra S,Shukla P,Bhaskar A,Anand K,Baloni P,Jha RK,Mohan A,Rajmani RS,Nagaraja V,Chandra N,Singh A

更新日期：2017-05-26 00:00:00