sRNA-mediated activation of gene expression by inhibition of 5'-3' exonucleolytic mRNA degradation.

abstract：:Trypanosome parasites are hiding in human skin, a discovery that may undermine efforts to eliminate sleeping sickness by 2020. ...

journal_title：eLife

authors： Casas-Sánchez A,Acosta-Serrano Á

更新日期：2016-10-14 00:00:00

abstract：:Cardiac conduction defects decrease life expectancy in myotonic dystrophy type 1 (DM1), a CTG repeat disorder involving misbalance between two RNA binding factors, MBNL1 and CELF1. However, how DM1 condition translates into conduction disorders remains poorly understood. Here we simulated MBNL1 and CELF1 misbalance in...

journal_title：eLife

authors： Auxerre-Plantié E,Nakamori M,Renaud Y,Huguet A,Choquet C,Dondi C,Miquerol L,Takahashi MP,Gourdon G,Junion G,Jagla T,Zmojdzian M,Jagla K

更新日期：2019-12-12 00:00:00

abstract：:Bioluminescence is a powerful biological signal that scientists have repurposed as a reporter for gene expression in plants and animals. However, there are downsides associated with the need to provide a substrate to these reporters, including its high cost and non-uniform tissue penetration. In this work we reconstit...

journal_title：eLife

authors： Khakhar A,Starker CG,Chamness JC,Lee N,Stokke S,Wang C,Swanson R,Rizvi F,Imaizumi T,Voytas DF

更新日期：2020-03-25 00:00:00

abstract：:The emergence of subpopulations that perform distinct metabolic roles has been observed in populations of genetically identical bacteria. ...

journal_title：eLife

authors： Dal Co A,Brannon C,Ackermann M

更新日期：2018-06-29 00:00:00

abstract：: ...

journal_title：eLife

authors： Zhou FY,Ruiz-Puig C,Owen RP,White MJ,Rittscher J,Lu X

更新日期：2019-07-02 00:00:00

abstract：:Oligomeric assemblies of intraflagellar transport (IFT) particles build cilia through sequential recruitment and transport of ciliary cargo proteins within cilia. Here we present the 1.8 Å resolution crystal structure of the Chlamydomonas IFT-B protein IFT80, which reveals the architecture of two N-terminal β-propelle...

journal_title：eLife

authors： Taschner M,Lorentzen A,Mourão A,Collins T,Freke GM,Moulding D,Basquin J,Jenkins D,Lorentzen E

更新日期：2018-04-16 00:00:00

abstract：:Surveillance strategies based on whole genome sequencing could help with the early identification and detection of new forms of drug-resistant gonorrhoea. ...

journal_title：eLife

authors： Medland N

更新日期：2020-06-30 00:00:00

abstract：:During embryogenesis, chromatin accessibility profiles control lineage-specific gene expression by modulating transcription, thus impacting multipotent progenitor states and subsequent fate choices. Subsets of cardiac and pharyngeal/head muscles share a common origin in the cardiopharyngeal mesoderm, but the chromatin...

journal_title：eLife

authors： Racioppi C,Wiechecki KA,Christiaen L

更新日期：2019-11-20 00:00:00

abstract：:We collated contact tracing data from COVID-19 clusters in Singapore and Tianjin, China and estimated the extent of pre-symptomatic transmission by estimating incubation periods and serial intervals. The mean incubation periods accounting for intermediate cases were 4.91 days (95%CI 4.35, 5.69) and 7.54 (95%CI 6.76, 8...

journal_title：eLife

authors： Tindale LC,Stockdale JE,Coombe M,Garlock ES,Lau WYV,Saraswat M,Zhang L,Chen D,Wallinga J,Colijn C

更新日期：2020-06-22 00:00:00

abstract：:Mature microRNAs (miRNAs) are processed from primary transcripts (pri-miRNAs), and their expression is controlled at transcriptional and post-transcriptional levels. However, how regulation at multiple levels achieves precise control remains elusive. Using published and new datasets, we profile a time course of mature...

journal_title：eLife

authors： Zhou L,Lim MYT,Kaur P,Saj A,Bortolamiol-Becet D,Gopal V,Tolwinski N,Tucker-Kellogg G,Okamura K

更新日期：2018-07-19 00:00:00

abstract：:WW domain tandem-containing proteins such as KIBRA, YAP, and MAGI play critical roles in cell growth and polarity via binding to and positioning target proteins in specific subcellular regions. An immense disparity exists between promiscuity of WW domain-mediated target bindings and specific roles of WW domain protein...

journal_title：eLife

authors： Lin Z,Yang Z,Xie R,Ji Z,Guan K,Zhang M

更新日期：2019-09-05 00:00:00

abstract：:Bacterial flagella mediate host-microbe interactions through tissue tropism during colonization, as well as by activating immune responses. The flagellar shaft of some bacteria, including several human pathogens, is encased in a membranous sheath of unknown function. While it has been hypothesized that the sheath may ...

journal_title：eLife

authors： Brennan CA,Hunt JR,Kremer N,Krasity BC,Apicella MA,McFall-Ngai MJ,Ruby EG

更新日期：2014-03-04 00:00:00

abstract：:Bacterial cells are encased in a peptidoglycan (PG) exoskeleton that protects them from osmotic lysis and specifies their distinct shapes. Cell wall hydrolases are required to enlarge this covalently closed macromolecule during growth, but how these autolytic enzymes are regulated remains poorly understood. Bacillus s...

journal_title：eLife

authors： Dobihal GS,Brunet YR,Flores-Kim J,Rudner DZ

更新日期：2019-12-06 00:00:00

abstract：:Mechanical force is a determinant of Notch signalling but the mechanism of force detection and its coupling to Notch are unclear. We propose a role for Piezo1 channels, which are mechanically-activated non-selective cation channels. In cultured microvascular endothelial cells, Piezo1 channel activation by either shear...

journal_title：eLife

authors： Caolo V,Debant M,Endesh N,Futers TS,Lichtenstein L,Bartoli F,Parsonage G,Jones EA,Beech DJ

更新日期：2020-06-02 00:00:00

abstract：:Variation in the presence or absence of transposable elements (TEs) is a major source of genetic variation between individuals. Here, we identified 23,095 TE presence/absence variants between 216 Arabidopsis accessions. Most TE variants were rare, and we find these rare variants associated with local extremes of gene ...

journal_title：eLife

authors： Stuart T,Eichten SR,Cahn J,Karpievitch YV,Borevitz JO,Lister R

更新日期：2016-12-02 00:00:00

abstract：:In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood. To understand the time-dependent scaling dynamics we treated cultured rat hippocampal cells wi...

journal_title：eLife

authors： Schanzenbächer CT,Langer JD,Schuman EM

更新日期：2018-02-15 00:00:00

abstract：:GPIHBP1, a GPI-anchored protein of capillary endothelial cells, binds lipoprotein lipase (LPL) within the subendothelial spaces and shuttles it to the capillary lumen. GPIHBP1-bound LPL is essential for the margination of triglyceride-rich lipoproteins (TRLs) along capillaries, allowing the lipolytic processing of TRL...

journal_title：eLife

authors： Hu X,Matsumoto K,Jung RS,Weston TA,Heizer PJ,He C,Sandoval NP,Allan CM,Tu Y,Vinters HV,Liau LM,Ellison RM,Morales JE,Baufeld LJ,Bayley NA,He L,Betsholtz C,Beigneux AP,Nathanson DA,Gerhardt H,Young SG,Fong LG,J

更新日期：2019-06-06 00:00:00

abstract：:Down syndrome (DS) results in various degrees of cognitive deficits. In DS mouse models, recovery of behavioral and neurophysiological deficits using GABAAR antagonists led to hypothesize an excessive activity of inhibitory circuits in this condition. Nonetheless, whether over-inhibition is present in DS and whether t...

journal_title：eLife

authors： Zorrilla de San Martin J,Donato C,Peixoto J,Aguirre A,Choudhary V,De Stasi AM,Lourenço J,Potier MC,Bacci A

更新日期：2020-08-12 00:00:00

abstract：:Establishing a link between the nonsense-mediated decay pathway and a gene associated with programmed cell death could explain why this pathway is essential in most, but not all, eukaryotes. ...

journal_title：eLife

authors： Saveanu C,Jacquier A

更新日期：2016-04-27 00:00:00

abstract：:The actomyosin ring generates force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organization and the mechanism of contractility is not well understood. We quantified actin filament order in human cells using fluorescence polarization microscopy and found that cleavage furrow ingression...

journal_title：eLife

authors： Spira F,Cuylen-Haering S,Mehta S,Samwer M,Reversat A,Verma A,Oldenbourg R,Sixt M,Gerlich DW

更新日期：2017-11-06 00:00:00

abstract：:Autoinducers are small signaling molecules that mediate intercellular communication in microbial populations and trigger coordinated gene expression via 'quorum sensing'. Elucidating the mechanisms that control autoinducer production is, thus, pertinent to understanding collective microbial behavior, such as virulence...

journal_title：eLife

authors： Bauer M,Knebel J,Lechner M,Pickl P,Frey E

更新日期：2017-07-25 00:00:00

abstract：:Neurons coding for head-direction are crucial for spatial navigation. Here we explored the cellular basis of head-direction coding in the rat dorsal presubiculum (PreS). We found that layer2 is composed of two principal cell populations (calbindin-positive and calbindin-negative neurons) which targeted the contralater...

journal_title：eLife

authors： Preston-Ferrer P,Coletta S,Frey M,Burgalossi A

更新日期：2016-06-10 00:00:00

abstract：:In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches ...

journal_title：eLife

authors： Yin Y,Dominska M,Yim E,Petes TD

更新日期：2017-07-17 00:00:00

abstract：:The immediate evolutionary space accessible to HIV is largely determined by how single amino acid mutations affect fitness. These mutational effects can shift as the virus evolves. However, the prevalence of such shifts in mutational effects remains unclear. Here, we quantify the effects on viral growth of all amino a...

journal_title：eLife

authors： Haddox HK,Dingens AS,Hilton SK,Overbaugh J,Bloom JD

更新日期：2018-03-28 00:00:00

abstract：:Mitogen-activated protein kinases (MAPK) such as p38 and the c-Jun N-terminal kinases (JNKs) are activated during the cellular response to stress signals. Their activity is regulated by the MAPK-phosphatase 1 (DUSP1), a key component of the anti-inflammatory response. Stress kinases are well-described elements of the ...

journal_title：eLife

authors： Celaya AM,Sánchez-Pérez I,Bermúdez-Muñoz JM,Rodríguez-de la Rosa L,Pintado-Berninches L,Perona R,Murillo-Cuesta S,Varela-Nieto I

更新日期：2019-04-02 00:00:00

abstract：:The circadian transcriptional repressors cryptochrome 1 (Cry1) and 2 (Cry2) evolved from photolyases, bacterial light-activated DNA repair enzymes. In this study, we report that while they have lost DNA repair activity, Cry1/2 adapted to protect genomic integrity by responding to DNA damage through posttranslational m...

journal_title：eLife

authors： Papp SJ,Huber AL,Jordan SD,Kriebs A,Nguyen M,Moresco JJ,Yates JR,Lamia KA

更新日期：2015-03-10 00:00:00

abstract：:Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric compon...

journal_title：eLife

authors： Spletter ML,Barz C,Yeroslaviz A,Zhang X,Lemke SB,Bonnard A,Brunner E,Cardone G,Basler K,Habermann BH,Schnorrer F

更新日期：2018-05-30 00:00:00

abstract：:Extracellular vesicles (EVs) encompass a variety of vesicles secreted into the extracellular space. EVs have been implicated in promoting tumor metastasis, but the molecular composition of tumor-derived EV sub-types and the mechanisms by which molecules are sorted into EVs remain mostly unknown. We report the separati...

journal_title：eLife

authors： Temoche-Diaz MM,Shurtleff MJ,Nottingham RM,Yao J,Fadadu RP,Lambowitz AM,Schekman R

更新日期：2019-08-22 00:00:00

abstract：:Many organisms lose the capacity to regenerate damaged tissues as they mature. Damaged Drosophila imaginal discs regenerate efficiently early in the third larval instar (L3) but progressively lose this ability. This correlates with reduced damage-responsive expression of multiple genes, including the WNT genes wingles...

journal_title：eLife

authors： Harris RE,Setiawan L,Saul J,Hariharan IK

更新日期：2016-02-03 00:00:00

abstract：:Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased in...

journal_title：eLife

authors： Wales P,Schuberth CE,Aufschnaiter R,Fels J,García-Aguilar I,Janning A,Dlugos CP,Schäfer-Herte M,Klingner C,Wälte M,Kuhlmann J,Menis E,Hockaday Kang L,Maier KC,Hou W,Russo A,Higgs HN,Pavenstädt H,Vogl T,Roth J,Qual

更新日期：2016-12-06 00:00:00