Genomics against gonorrhoea.

abstract：:Recent transcriptional profiling technologies are uncovering previously-undefined cell populations and molecular markers at an unprecedented pace. While single cell RNA (scRNA) sequencing is an attractive approach for unbiased transcriptional profiling of all cell types, a complementary method to isolate and sequence ...

journal_title：eLife

authors： Amamoto R,Garcia MD,West ER,Choi J,Lapan SW,Lane EA,Perrimon N,Cepko CL

更新日期：2019-12-09 00:00:00

abstract：:The dynamic tyrosination-detyrosination cycle of α-tubulin regulates microtubule functions. Perturbation of this cycle impairs mitosis, neural physiology, and cardiomyocyte contraction. The carboxypeptidases vasohibins 1 and 2 (VASH1 and VASH2), in complex with the small vasohibin-binding protein (SVBP), mediate α-tub...

journal_title：eLife

authors： Li F,Li Y,Ye X,Gao H,Shi Z,Luo X,Rice LM,Yu H

更新日期：2020-08-10 00:00:00

abstract：:In plants the dorsoventral boundary of leaves defines an axis of symmetry through the centre of the organ separating the top (dorsal) and bottom (ventral) tissues. Although the positioning of this boundary is critical for leaf morphogenesis, how the boundary is established and how it influences development remains unc...

journal_title：eLife

authors： Caggiano MP,Yu X,Bhatia N,Larsson A,Ram H,Ohno CK,Sappl P,Meyerowitz EM,Jönsson H,Heisler MG

更新日期：2017-09-12 00:00:00

abstract：:In vivo, the primary molecular mechanotransductive events mechanically initiating cell differentiation remain unknown. Here we find the molecular stretching of the highly conserved Y654-β-catenin-D665-E-cadherin binding site as mechanically induced by tissue strain. It triggers the increase of accessibility of the Y65...

journal_title：eLife

authors： Röper JC,Mitrossilis D,Stirnemann G,Waharte F,Brito I,Fernandez-Sanchez ME,Baaden M,Salamero J,Farge E

更新日期：2018-07-19 00:00:00

abstract：:In many daily tasks, we make multiple decisions before reaching a goal. In order to learn such sequences of decisions, a mechanism to link earlier actions to later reward is necessary. Reinforcement learning (RL) theory suggests two classes of algorithms solving this credit assignment problem: In classic temporal-diff...

journal_title：eLife

authors： Lehmann MP,Xu HA,Liakoni V,Herzog MH,Gerstner W,Preuschoff K

更新日期：2019-11-11 00:00:00

abstract：:Mu-opioid receptors (MORs) are crucial for analgesia by both exogenous and endogenous opioids. However, the distinct mechanisms underlying these two types of opioid analgesia remain largely unknown. Here, we demonstrate that analgesic effects of exogenous and endogenous opioids on inflammatory pain are mediated by MOR...

journal_title：eLife

authors： Zhang XY,Dou YN,Yuan L,Li Q,Zhu YJ,Wang M,Sun YG

更新日期：2020-06-10 00:00:00

abstract：:Most evolutionary thinking is based on the notion of fitness and related ideas such as fitness landscapes and evolutionary optima. Nevertheless, it is often unclear what fitness actually is, and its meaning often depends on the context. Here we argue that fitness should not be a basal ingredient in verbal or mathemati...

journal_title：eLife

authors： Doebeli M,Ispolatov Y,Simon B

更新日期：2017-02-15 00:00:00

abstract：:Systemic metabolic reprogramming induced by infection exerts profound, pathogen-specific effects on infection outcome. Here, we detail the host immune and metabolic response during sickness and recovery in a mouse model of malaria. We describe extensive alterations in metabolism during acute infection, and identify in...

journal_title：eLife

authors： Lissner MM,Cumnock K,Davis NM,Vilches-Moure JG,Basak P,Navarrete DJ,Allen JA,Schneider D

更新日期：2020-10-06 00:00:00

abstract：:Genomic rearrangements associated with speciation often result in variation in chromosome number among closely related species. Malassezia species show variable karyotypes ranging between six and nine chromosomes. Here, we experimentally identified all eight centromeres in M. sympodialis as 3-5-kb long kinetochore-bou...

journal_title：eLife

authors： Sankaranarayanan SR,Ianiri G,Coelho MA,Reza MH,Thimmappa BC,Ganguly P,Vadnala RN,Sun S,Siddharthan R,Tellgren-Roth C,Dawson TL Jnr,Heitman J,Sanyal K

更新日期：2020-01-20 00:00:00

abstract：:A fluorescent imaging technique called fastFISH has been used to track the various steps involved in the transcription of a single DNA molecule. ...

journal_title：eLife

authors： Monnet J,Strick TR

更新日期：2014-01-01 00:00:00

abstract：:How does attentional modulation of neural activity enhance performance? Here we use a deep convolutional neural network as a large-scale model of the visual system to address this question. We model the feature similarity gain model of attention, in which attentional modulation is applied according to neural stimulus ...

journal_title：eLife

authors： Lindsay GW,Miller KD

更新日期：2018-10-01 00:00:00

abstract：:The loss of previously adaptive traits is typically linked to relaxation in selection, yet the molecular steps leading to such repeated losses are rarely known. Molecular studies of loss have tended to focus on gene sequences alone, but overlooking other aspects of protein expression might underestimate phenotypic div...

journal_title：eLife

authors： Sadier A,Davies KT,Yohe LR,Yun K,Donat P,Hedrick BP,Dumont ER,Dávalos LM,Rossiter SJ,Sears KE

更新日期：2018-12-18 00:00:00

abstract：:Gap junctions are widely distributed in the brains across species and play essential roles in neural information processing. However, the role of gap junctions in insect cognition remains poorly understood. Using a flight simulator paradigm and genetic tools, we found that gap junctions are present in Drosophila Kenyo...

journal_title：eLife

authors： Liu Q,Yang X,Tian J,Gao Z,Wang M,Li Y,Guo A

更新日期：2016-05-24 00:00:00

abstract：:Cell-cell communication in multicellular organisms depends on the dynamic and reversible phosphorylation of protein tyrosine residues. The receptor-linked protein tyrosine phosphatases (RPTPs) receive cues from the extracellular environment and are well placed to influence cell signaling. However, the direct events do...

journal_title：eLife

authors： Fearnley GW,Young KA,Edgar JR,Antrobus R,Hay IM,Liang WC,Martinez-Martin N,Lin W,Deane JE,Sharpe HJ

更新日期：2019-03-29 00:00:00

abstract：:MFN2 encodes mitofusin 2, a membrane-bound mediator of mitochondrial membrane fusion and inter-organelle communication. MFN2 mutations cause axonal neuropathy, with associated lipodystrophy only occasionally noted, however homozygosity for the p.Arg707Trp mutation was recently associated with upper body adipose overgr...

journal_title：eLife

authors： Rocha N,Bulger DA,Frontini A,Titheradge H,Gribsholt SB,Knox R,Page M,Harris J,Payne F,Adams C,Sleigh A,Crawford J,Gjesing AP,Bork-Jensen J,Pedersen O,Barroso I,Hansen T,Cox H,Reilly M,Rossor A,Brown RJ,Taylor SI

更新日期：2017-04-19 00:00:00

abstract：:Animals seek out relevant information by moving through a dynamic world, but sensory systems are usually studied under highly constrained and passive conditions that may not probe important dimensions of the neural code. Here, we explored neural coding in the barrel cortex of head-fixed mice that tracked walls with th...

journal_title：eLife

authors： Sofroniew NJ,Vlasov YA,Hires SA,Freeman J,Svoboda K

更新日期：2015-12-23 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) have been shown to act as important cell biological regulators including cell fate decisions but are often ignored in human genetics. Combining differential lncRNA expression during neuronal lineage induction with copy number variation morbidity maps of a cohort of children with autism sp...

journal_title：eLife

authors： Ang CE,Ma Q,Wapinski OL,Fan S,Flynn RA,Lee QY,Coe B,Onoguchi M,Olmos VH,Do BT,Dukes-Rimsky L,Xu J,Tanabe K,Wang L,Elling U,Penninger JM,Zhao Y,Qu K,Eichler EE,Srivastava A,Wernig M,Chang HY

更新日期：2019-01-10 00:00:00

abstract：:Methyl-coenzyme M reductase (MCR), found in strictly anaerobic methanogenic and methanotrophic archaea, catalyzes the reversible production and consumption of the potent greenhouse gas methane. The α subunit of MCR (McrA) contains several unusual post-translational modifications, including a rare thioamidation of glyc...

journal_title：eLife

authors： Nayak DD,Mahanta N,Mitchell DA,Metcalf WW

更新日期：2017-09-07 00:00:00

abstract：:A strain of budding yeast that contains one large chromosome reveals how the telomere capping complex CST maintains linear but not circular chromosomes. ...

journal_title：eLife

authors： Nugent C,Sugimoto K

更新日期：2020-08-05 00:00:00

abstract：:The transcription factor TCF7L1 is an embryonic stem cell signature gene that is upregulated in multiple aggressive cancer types, but its role in skin tumorigenesis has not yet been defined. Here we document TCF7L1 upregulation in skin squamous cell carcinoma (SCC) and demonstrate that TCF7L1 overexpression increases ...

journal_title：eLife

authors： Ku AT,Shaver TM,Rao AS,Howard JM,Rodriguez CN,Miao Q,Garcia G,Le D,Yang D,Borowiak M,Cohen DN,Chitsazzadeh V,Diwan AH,Tsai KY,Nguyen H

更新日期：2017-05-03 00:00:00

abstract：:The dendritic cell receptor Clec9A facilitates processing of dead cell-derived antigens for cross-presentation and the induction of effective CD8+ T cell immune responses. Here, we show that this process is regulated by E3 ubiquitin ligase RNF41 and define a new ubiquitin-mediated mechanism for regulation of Clec9A, r...

journal_title：eLife

authors： Tullett KM,Tan PS,Park HY,Schittenhelm RB,Michael N,Li R,Policheni AN,Gruber E,Huang C,Fulcher AJ,Danne JC,Czabotar PE,Wakim LM,Mintern JD,Ramm G,Radford KJ,Caminschi I,O'Keeffe M,Villadangos JA,Wright MD,Blewitt

更新日期：2020-12-02 00:00:00

abstract：:Human-based modelling and simulations are becoming ubiquitous in biomedical science due to their ability to augment experimental and clinical investigations. Cardiac electrophysiology is one of the most advanced areas, with cardiac modelling and simulation being considered for virtual testing of pharmacological therap...

journal_title：eLife

authors： Tomek J,Bueno-Orovio A,Passini E,Zhou X,Minchole A,Britton O,Bartolucci C,Severi S,Shrier A,Virag L,Varro A,Rodriguez B

更新日期：2019-12-24 00:00:00

abstract：:Hippo signalling integrates diverse stimuli related to epithelial architecture to regulate tissue growth and cell fate decisions. The Hippo kinase cascade represses the growth-promoting transcription co-activator Yorkie. The FERM protein Expanded is one of the main upstream Hippo signalling regulators in Drosophila as...

journal_title：eLife

authors： Fulford AD,Holder MV,Frith D,Snijders AP,Tapon N,Ribeiro PS

更新日期：2019-09-30 00:00:00

abstract：:A well-established cascade of transcription factor (TF) activity orchestrates adipogenesis in response to chemical cues, yet how cell-intrinsic determinants of differentiation such as cell shape and/or seeding density inform this transcriptional program remain enigmatic. Here, we uncover a novel mechanism licensing tr...

journal_title：eLife

authors： Cohen DM,Won KJ,Nguyen N,Lazar MA,Chen CS,Steger DJ

更新日期：2015-06-25 00:00:00

abstract：:Cell surface reception of Sonic hedgehog (Shh) must ensure that the graded morphogenic signal is interpreted accordingly in neighboring cells to specify tissue patterns during development. Here, we report endocytic sorting signals for the receptor Patched1 (Ptch1), comprising two 'PPXY' motifs, that direct it to degra...

journal_title：eLife

authors： Yue S,Tang LY,Tang Y,Tang Y,Shen QH,Ding J,Chen Y,Zhang Z,Yu TT,Zhang YE,Cheng SY

更新日期：2014-06-12 00:00:00

abstract：:Recurrent somatic mutations of H3F3A in aggressive pediatric high-grade gliomas generate K27M or G34R/V mutant histone H3.3. H3.3-G34R/V mutants are common in tumors with mutations in p53 and ATRX, an H3.3-specific chromatin remodeler. To gain insight into the role of H3-G34R, we generated fission yeast that express o...

journal_title：eLife

authors： Yadav RK,Jablonowski CM,Fernandez AG,Lowe BR,Henry RA,Finkelstein D,Barnum KJ,Pidoux AL,Kuo YM,Huang J,O'Connell MJ,Andrews AJ,Onar-Thomas A,Allshire RC,Partridge JF

更新日期：2017-07-18 00:00:00

abstract：:The choroid, which provides vascular supply to the outer retina, demonstrates progressive degeneration in aging and age-related macular degeneration (AMD). However mechanisms that maintain or compromise choroidal homeostasis are obscure. We discovered that the ablation of choroidal macrophages via CSF1R blockade was a...

journal_title：eLife

authors： Yang X,Zhao L,Campos MM,Abu-Asab M,Ortolan D,Hotaling N,Bharti K,Wong WT

更新日期：2020-04-01 00:00:00

abstract：:Selective autophagy is mediated by cargo receptors that link the cargo to the isolation membrane via interactions with Atg8 proteins. Atg8 proteins are localized to the membrane in an ubiquitin-like conjugation reaction, but how this conjugation is coupled to the presence of the cargo is unclear. Here we show that the...

journal_title：eLife

authors： Fracchiolla D,Sawa-Makarska J,Zens B,Ruiter A,Zaffagnini G,Brezovich A,Romanov J,Runggatscher K,Kraft C,Zagrovic B,Martens S

更新日期：2016-11-23 00:00:00

abstract：:We previously showed in Drosophila melanogaster embryos that low-affinity Ultrabithorax (Ubx)-responsive shavenbaby (svb) enhancers drive expression using localized transcriptional environments and that active svb enhancers on different chromosomes tended to colocalize (Tsai et al., 2017). Here, we test the hypothesis...

journal_title：eLife

authors： Tsai A,Alves MR,Crocker J

更新日期：2019-07-11 00:00:00

abstract：:The sensitivity of ocular dominance to regulation by monocular deprivation is the canonical model of plasticity confined to a critical period. However, we have previously shown that visual deprivation through dark exposure (DE) reactivates critical period plasticity in adults. Previous work assumed that the eliminatio...

journal_title：eLife

authors： Murase S,Lantz CL,Quinlan EM

更新日期：2017-09-06 00:00:00