Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments.

abstract：:Negative feedback regulation, that is the ability of a gene to repress its own synthesis, is the most abundant regulatory motif known to biology. Frequently reported for transcriptional regulators, negative feedback control relies on binding of a transcription factor to its own promoter. Here, we report a novel mechan...

journal_title：eLife

authors： Hoyos M,Huber M,Förstner KU,Papenfort K

更新日期：2020-08-03 00:00:00

abstract：:While the dynamin GTPase Drp1 plays a critical role during mitochondrial fission, mechanisms controlling its recruitment to fission sites are unclear. A current assumption is that cytosolic Drp1 is recruited directly to fission sites immediately prior to fission. Using live-cell microscopy, we find evidence for a diff...

journal_title：eLife

authors： Ji WK,Hatch AL,Merrill RA,Strack S,Higgs HN

更新日期：2015-11-26 00:00:00

abstract：:To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm genera...

journal_title：eLife

authors： Wang X,Hayes JA,Revill AL,Song H,Kottick A,Vann NC,LaMar MD,Picardo MC,Akins VT,Funk GD,Del Negro CA

更新日期：2014-07-15 00:00:00

abstract：:Polarity is a shared feature of most cells. In epithelia, apical-basal polarity often coexists, and sometimes intersects with planar cell polarity (PCP), which orients cells in the epithelial plane. From a limited set of core building blocks (e.g. the Par complexes for apical-basal polarity and the Frizzled/Dishevelle...

journal_title：eLife

authors： Banerjee JJ,Aerne BL,Holder MV,Hauri S,Gstaiger M,Tapon N

更新日期：2017-06-30 00:00:00

abstract：:Recent advances in single-cell transcriptomics techniques have opened the door to the study of gene regulatory networks (GRNs) at the single-cell level. Here, we studied the GRNs controlling the emergence of hematopoietic stem and progenitor cells from mouse embryonic endothelium using a combination of single-cell tra...

journal_title：eLife

authors： Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

更新日期：2018-03-20 00:00:00

abstract：:Transposable elements (TEs), the movement of which can damage the genome, are epigenetically silenced in eukaryotes. Intriguingly, TEs are activated in the sperm companion cell - vegetative cell (VC) - of the flowering plant Arabidopsis thaliana. However, the extent and mechanism of this activation are unknown. Here w...

journal_title：eLife

authors： He S,Vickers M,Zhang J,Feng X

更新日期：2019-05-28 00:00:00

abstract：:The roles of long non-coding RNAs (lncRNAs) in regulating cancer and stem cells are being increasingly appreciated. Its diverse mechanisms provide the regulatory network with a bigger repertoire to increase complexity. Here we report a novel LncRNA, Lnc34a, that is enriched in colon cancer stem cells (CCSCs) and initi...

journal_title：eLife

authors： Wang L,Bu P,Ai Y,Srinivasan T,Chen HJ,Xiang K,Lipkin SM,Shen X

更新日期：2016-04-14 00:00:00

abstract：:Proteins can self-organize into spatial patterns via non-linear dynamic interactions on cellular membranes. Modelling and simulations have shown that small GTPases can generate patterns by coupling guanine nucleotide exchange factors (GEF) to effectors, generating a positive feedback of GTPase activation and membrane ...

journal_title：eLife

authors： Cezanne A,Lauer J,Solomatina A,Sbalzarini IF,Zerial M

更新日期：2020-06-08 00:00:00

abstract：:Experience-dependent modifications of synaptic connections are thought to change patterns of network activities and stimulus tuning with learning. However, only a few studies explored how synaptic plasticity shapes the response dynamics of cortical circuits. Here, we investigated the mechanism underlying sharpening of...

journal_title：eLife

authors： Lim S

更新日期：2019-08-08 00:00:00

abstract：:The human face represents a combined set of highly heritable phenotypes, but knowledge on its genetic architecture remains limited, despite the relevance for various fields. A series of genome-wide association studies on 78 facial shape phenotypes quantified from 3-dimensional facial images of 10,115 Europeans identif...

journal_title：eLife

authors： Xiong Z,Dankova G,Howe LJ,Lee MK,Hysi PG,de Jong MA,Zhu G,Adhikari K,Li D,Li Y,Pan B,Feingold E,Marazita ML,Shaffer JR,McAloney K,Xu SH,Jin L,Wang S,de Vrij FM,Lendemeijer B,Richmond S,Zhurov A,Lewis S,Sharp

更新日期：2019-11-26 00:00:00

abstract：:The calcium release-activated calcium channel Orai regulates Ca2+ entry into non-excitable cells and is required for proper immune function. While the channel typically opens following Ca2+ release from the endoplasmic reticulum, certain pathologic mutations render the channel constitutively open. Previously, using on...

journal_title：eLife

authors： Hou X,Outhwaite IR,Pedi L,Long SB

更新日期：2020-11-30 00:00:00

abstract：:Aedes aegypti transmit pathogenic arboviruses while the mosquito itself tolerates the infection. We examine a piRNA-based immunity that relies on the acquisition of viral derived cDNA (vDNA) and how this pathway discriminates between self and non-self. The piRNAs derived from these vDNAs are essential for virus contro...

journal_title：eLife

authors： Tassetto M,Kunitomi M,Whitfield ZJ,Dolan PT,Sánchez-Vargas I,Garcia-Knight M,Ribiero I,Chen T,Olson KE,Andino R

更新日期：2019-10-17 00:00:00

abstract：:Repression of genes by Polycomb requires that PRC2 modifies their chromatin by trimethylating lysine 27 on histone H3 (H3K27me3). At transcriptionally active genes, di- and tri-methylated H3K36 inhibit PRC2. Here, the cryo-EM structure of PRC2 on dinucleosomes reveals how binding of its catalytic subunit EZH2 to nucle...

journal_title：eLife

authors： Finogenova K,Bonnet J,Poepsel S,Schäfer IB,Finkl K,Schmid K,Litz C,Strauss M,Benda C,Müller J

更新日期：2020-11-19 00:00:00

abstract：:Evolution persistently undermines vector control programs through insecticide resistance. Here we propose a novel strategy which instead exploits evolution to generate and sustain new control tools. Effective spatial repellents are needed to keep vectors out of houses. Our approach generates such new repellents by com...

journal_title：eLife

authors： Lynch PA,Boots M

更新日期：2016-10-25 00:00:00

abstract：:We studied the role of the synaptic ribbon for sound encoding at the synapses between inner hair cells (IHCs) and spiral ganglion neurons (SGNs) in mice lacking RIBEYE (RBEKO/KO). Electron and immunofluorescence microscopy revealed a lack of synaptic ribbons and an assembly of several small active zones (AZs) at each ...

journal_title：eLife

authors： Jean P,Lopez de la Morena D,Michanski S,Jaime Tobón LM,Chakrabarti R,Picher MM,Neef J,Jung S,Gültas M,Maxeiner S,Neef A,Wichmann C,Strenzke N,Grabner C,Moser T

更新日期：2018-01-12 00:00:00

abstract：:C-type inactivation of potassium channels fine-tunes the electrical signaling in excitable cells through an internal timing mechanism that is mediated by a hydrogen bond network in the channels' selectively filter. Previously, we used nonsense suppression to highlight the role of the conserved Trp434-Asp447 indole hyd...

journal_title：eLife

authors： Lueck JD,Mackey AL,Infield DT,Galpin JD,Li J,Roux B,Ahern CA

更新日期：2016-10-06 00:00:00

abstract：:In Drosophila larvae, Class IV sensory neurons respond to noxious thermal stimuli and provoke heat avoidance behavior. Previously, we showed that the activated neurons displayed characteristic fluctuations of firing rates, which consisted of repetitive high-frequency spike trains and subsequent pause periods, and we p...

journal_title：eLife

authors： Onodera K,Baba S,Murakami A,Uemura T,Usui T

更新日期：2017-10-16 00:00:00

abstract：:Synaptic adhesion molecules regulate synapse development and function. However, whether and how presynaptic adhesion molecules regulate postsynaptic NMDAR function remains largely unclear. Presynaptic LAR family receptor tyrosine phosphatases (LAR-RPTPs) regulate synapse development through mechanisms that include tra...

journal_title：eLife

authors： Kim K,Shin W,Kang M,Lee S,Kim D,Kang R,Jung Y,Cho Y,Yang E,Kim H,Bae YC,Kim E

更新日期：2020-03-06 00:00:00

abstract：:Pore-blocking toxins inhibit voltage-dependent K(+) channels (Kv channels) by plugging the ion-conduction pathway. We have solved the crystal structure of paddle chimera, a Kv channel in complex with charybdotoxin (CTX), a pore-blocking toxin. The toxin binds to the extracellular pore entryway without producing discer...

journal_title：eLife

authors： Banerjee A,Lee A,Campbell E,Mackinnon R

更新日期：2013-05-21 00:00:00

abstract：:Asymmetric disassembly of the synaptonemal complex (SC) is crucial for proper meiotic chromosome segregation. However, the signaling mechanisms that directly regulate this process are poorly understood. Here we show that the mammalian Rho GEF homolog, ECT-2, functions through the conserved RAS/ERK MAP kinase signaling...

journal_title：eLife

authors： Nadarajan S,Mohideen F,Tzur YB,Ferrandiz N,Crawley O,Montoya A,Faull P,Snijders AP,Cutillas PR,Jambhekar A,Blower MD,Martinez-Perez E,Harper JW,Colaiacovo MP

更新日期：2016-02-27 00:00:00

abstract：:Seasonal influenza virus A/H3N2 is a major cause of death globally. Vaccination remains the most effective preventative. Rapid mutation of hemagglutinin allows viruses to escape adaptive immunity. This antigenic drift necessitates regular vaccine updates. Effective vaccine strains need to represent H3N2 populations ci...

journal_title：eLife

authors： Huddleston J,Barnes JR,Rowe T,Xu X,Kondor R,Wentworth DE,Whittaker L,Ermetal B,Daniels RS,McCauley JW,Fujisaki S,Nakamura K,Kishida N,Watanabe S,Hasegawa H,Barr I,Subbarao K,Barrat-Charlaix P,Neher RA,Bedford T

更新日期：2020-09-02 00:00:00

abstract：:It is now clear that microglia and macrophages are present in brain tumors, but whether or how they affect initiation and development of tumors is not known. Exploiting the advantages of the zebrafish (Danio rerio) model, we showed that macrophages and microglia respond immediately upon oncogene activation in the brai...

journal_title：eLife

authors： Chia K,Mazzolini J,Mione M,Sieger D

更新日期：2018-02-21 00:00:00

abstract：:A major challenge in neuroscience is to longitudinally monitor whole brain activity across multiple spatial scales in the same animal. Functional UltraSound (fUS) is an emerging technology that offers images of cerebral blood volume over large brain portions. Here we show for the first time its capability to resolve t...

journal_title：eLife

authors： Bimbard C,Demene C,Girard C,Radtke-Schuller S,Shamma S,Tanter M,Boubenec Y

更新日期：2018-06-28 00:00:00

abstract：:Many postdoctoral researchers apply for faculty positions knowing relatively little about the hiring process or what is needed to secure a job offer. To address this lack of knowledge about the hiring process we conducted a survey of applicants for faculty positions: the survey ran between May 2018 and May 2019, and r...

journal_title：eLife

authors： Fernandes JD,Sarabipour S,Smith CT,Niemi NM,Jadavji NM,Kozik AJ,Holehouse AS,Pejaver V,Symmons O,Bisson Filho AW,Haage A

更新日期：2020-06-12 00:00:00

abstract：:Generating recombinant monoclonal antibodies (R-mAbs) from mAb-producing hybridomas offers numerous advantages that increase the effectiveness, reproducibility, and transparent reporting of research. We report here the generation of a novel resource in the form of a library of recombinant R-mAbs validated for neurosci...

journal_title：eLife

authors： Andrews NP,Boeckman JX,Manning CF,Nguyen JT,Bechtold H,Dumitras C,Gong B,Nguyen K,van der List D,Murray KD,Engebrecht J,Trimmer JS

更新日期：2019-01-22 00:00:00

abstract：:Aging is an intricate phenomenon associated with the gradual loss of physiological functions, and both nutrient sensing and proteostasis control lifespan. Although multiple approaches have facilitated the identification of candidate genes that govern longevity, the molecular mechanisms that link aging pathways are sti...

journal_title：eLife

authors： Chen YC,Jiang PH,Chen HM,Chen CH,Wang YT,Chen YJ,Yu CJ,Teng SC

更新日期：2018-12-05 00:00:00

abstract：:Mature microRNAs (miRNAs) are processed from primary transcripts (pri-miRNAs), and their expression is controlled at transcriptional and post-transcriptional levels. However, how regulation at multiple levels achieves precise control remains elusive. Using published and new datasets, we profile a time course of mature...

journal_title：eLife

authors： Zhou L,Lim MYT,Kaur P,Saj A,Bortolamiol-Becet D,Gopal V,Tolwinski N,Tucker-Kellogg G,Okamura K

更新日期：2018-07-19 00:00:00

abstract：:Negative regulators of adult neurogenesis are of particular interest as targets to enhance neuronal repair, but few have yet been identified. Planarians can regenerate their entire CNS using pluripotent adult stem cells, and this process is robustly regulated to ensure that new neurons are produced in proper abundance...

journal_title：eLife

authors： Karge A,Bonar NA,Wood S,Petersen CP

更新日期：2020-01-20 00:00:00

abstract：:Early life adversity (ELA) is associated with increased risk for stress-related disorders later in life. The link between ELA and risk for psychopathology is well established but the developmental mechanisms remain unclear. Using a mouse model of resource insecurity, limited bedding (LB), we tested the effects of LB o...

journal_title：eLife

authors： Manzano Nieves G,Bravo M,Baskoylu S,Bath KG

更新日期：2020-07-21 00:00:00

abstract：:Phosphorylation of eIF2α controls translation initiation by restricting the levels of active eIF2-GTP/Met-tRNAi ternary complexes (TC). This modulates the expression of all eukaryotic mRNAs and contributes to the cellular integrated stress response. Key to controlling the activity of eIF2 are translation factors eIF2B...

journal_title：eLife

authors： Jennings MD,Kershaw CJ,Adomavicius T,Pavitt GD

更新日期：2017-03-18 00:00:00