Abstract:
:To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm generator in mammals are derived from Dbx1-expressing precursors. Here, we show that selective photonic destruction of Dbx1 preBötC neurons in neonatal mouse slices impairs respiratory rhythm but surprisingly also the magnitude of motor output; respiratory hypoglossal nerve discharge decreased and its frequency steadily diminished until rhythm stopped irreversibly after 85±20 (mean ± SEM) cellular ablations, which corresponds to ∼15% of the estimated population. These results demonstrate that a single canonical interneuron class generates respiratory rhythm and contributes in a premotor capacity, whereas these functions are normally attributed to discrete populations. We also establish quantitative cellular parameters that govern network viability, which may have ramifications for respiratory pathology in disease states.
journal_name
Elifejournal_title
eLifeauthors
Wang X,Hayes JA,Revill AL,Song H,Kottick A,Vann NC,LaMar MD,Picardo MC,Akins VT,Funk GD,Del Negro CAdoi
10.7554/eLife.03427subject
Has Abstractpub_date
2014-07-15 00:00:00pages
e03427issn
2050-084Xjournal_volume
3pub_type
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