Assembling the Tat protein translocase.

abstract：:A hallmark of CRISPR-Cas immunity systems is the CRISPR array, a genomic locus consisting of short, repeated sequences ('repeats') interspersed with short, variable sequences ('spacers'). CRISPR arrays are transcribed and processed into individual CRISPR RNAs that each include a single spacer, and direct Cas proteins ...

journal_title：eLife

authors： Stringer AM,Baniulyte G,Lasek-Nesselquist E,Seed KD,Wade JT

更新日期：2020-10-30 00:00:00

abstract：:Two different cell signals often affect transcription of the same gene. In such cases, it is natural to ask how the combined transcriptional response compares to the individual responses. The most commonly used mechanistic models predict additive or multiplicative combined responses, but a systematic genome-wide evalu...

journal_title：eLife

authors： Sanford EM,Emert BL,Coté A,Raj A

更新日期：2020-12-07 00:00:00

abstract：:Two mammalian genes, Kcnt1 and Kcnt2, encode pore-forming subunits of Na(+)-dependent K(+) (KNa) channels. Progress in understanding KNa channels has been hampered by the absence of specific tools and methods for rigorous KNa identification in native cells. Here, we report the genetic disruption of both Kcnt1 and Kcnt...

journal_title：eLife

authors： Martinez-Espinosa PL,Wu J,Yang C,Gonzalez-Perez V,Zhou H,Liang H,Xia XM,Lingle CJ

更新日期：2015-11-11 00:00:00

abstract：:Cell surface reception of Sonic hedgehog (Shh) must ensure that the graded morphogenic signal is interpreted accordingly in neighboring cells to specify tissue patterns during development. Here, we report endocytic sorting signals for the receptor Patched1 (Ptch1), comprising two 'PPXY' motifs, that direct it to degra...

journal_title：eLife

authors： Yue S,Tang LY,Tang Y,Tang Y,Shen QH,Ding J,Chen Y,Zhang Z,Yu TT,Zhang YE,Cheng SY

更新日期：2014-06-12 00:00:00

abstract：:GPIHBP1, a GPI-anchored protein of capillary endothelial cells, binds lipoprotein lipase (LPL) within the subendothelial spaces and shuttles it to the capillary lumen. GPIHBP1-bound LPL is essential for the margination of triglyceride-rich lipoproteins (TRLs) along capillaries, allowing the lipolytic processing of TRL...

journal_title：eLife

authors： Hu X,Matsumoto K,Jung RS,Weston TA,Heizer PJ,He C,Sandoval NP,Allan CM,Tu Y,Vinters HV,Liau LM,Ellison RM,Morales JE,Baufeld LJ,Bayley NA,He L,Betsholtz C,Beigneux AP,Nathanson DA,Gerhardt H,Young SG,Fong LG,J

更新日期：2019-06-06 00:00:00

abstract：:Understanding how virus capsids assemble around their nucleic acid (NA) genomes could promote efforts to block viral propagation or to reengineer capsids for gene therapy applications. We develop a coarse-grained model of capsid proteins and NAs with which we investigate assembly dynamics and thermodynamics. In contra...

journal_title：eLife

authors： Perlmutter JD,Qiao C,Hagan MF

更新日期：2013-06-14 00:00:00

abstract：:Unrepaired DNA damage during embryonic development can be potentially inherited by a large population of cells. However, the quality control mechanisms that minimize the contribution of damaged cells to developing embryos remain poorly understood. Here, we uncovered an ATR- and CHK1-mediated transcriptional response t...

journal_title：eLife

authors： Atashpaz S,Samadi Shams S,Gonzalez JM,Sebestyén E,Arghavanifard N,Gnocchi A,Albers E,Minardi S,Faga G,Soffientini P,Allievi E,Cancila V,Bachi A,Fernández-Capetillo Ó,Tripodo C,Ferrari F,López-Contreras AJ,Costanzo V

更新日期：2020-03-12 00:00:00

abstract：:An 'interactome' screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interacto...

journal_title：eLife

authors： Li H,Watson A,Olechwier A,Anaya M,Sorooshyari SK,Harnett DP,Lee HP,Vielmetter J,Fares MA,Garcia KC,Özkan E,Labrador JP,Zinn K

更新日期：2017-08-15 00:00:00

abstract：:Nucleolar protein interacting with the FHA domain of pKi-67 (NIFK) is a Ki-67-interacting protein. However, its precise function in cancer remains largely uninvestigated. Here we show the clinical significance and metastatic mechanism of NIFK in lung cancer. NIFK expression is clinically associated with poor prognosis...

journal_title：eLife

authors： Lin TC,Su CY,Wu PY,Lai TC,Pan WA,Jan YH,Chang YC,Yeh CT,Chen CL,Ger LP,Chang HT,Yang CJ,Huang MS,Liu YP,Lin YF,Shyy JY,Tsai MD,Hsiao M

更新日期：2016-03-17 00:00:00

abstract：:Analysis of the spatial distribution of endomembrane trafficking is fundamental to understand the mechanisms controlling cellular dynamics, cell homeostasy, and cell interaction with its external environment in normal and pathological situations. We present a semi-parametric framework to quantitatively analyze and vis...

journal_title：eLife

authors： Pécot T,Zengzhen L,Boulanger J,Salamero J,Kervrann C

更新日期：2018-08-09 00:00:00

abstract：:Our understanding of the antigen presentation pathway has recently been enhanced with the identification that the tapasin-related protein TAPBPR is a second major histocompatibility complex (MHC) class I-specific chaperone. We sought to determine whether, like tapasin, TAPBPR can also influence MHC class I peptide sel...

journal_title：eLife

authors： Hermann C,van Hateren A,Trautwein N,Neerincx A,Duriez PJ,Stevanović S,Trowsdale J,Deane JE,Elliott T,Boyle LH

更新日期：2015-10-06 00:00:00

abstract：:While host immune receptors detect pathogen-associated molecular patterns to activate immunity, pathogens attempt to deregulate host immunity through secreted effectors. Fungi employ LysM effectors to prevent recognition of cell wall-derived chitin by host immune receptors, although the mechanism to compete for chitin...

journal_title：eLife

authors： Sánchez-Vallet A,Saleem-Batcha R,Kombrink A,Hansen G,Valkenburg DJ,Thomma BP,Mesters JR

更新日期：2013-07-02 00:00:00

abstract：:Human SEMAPHORIN 5A (SEMA5A) is an autism susceptibility gene; however, its function in brain development is unknown. In this study, we show that mouse Sema5A negatively regulates synaptogenesis in early, developmentally born, hippocampal dentate granule cells (GCs). Sema5A is strongly expressed by GCs and regulates d...

journal_title：eLife

authors： Duan Y,Wang SH,Song J,Mironova Y,Ming GL,Kolodkin AL,Giger RJ

更新日期：2014-10-14 00:00:00

abstract：:Gene copy number alterations, tumor cell stemness, and the development of platinum chemotherapy resistance contribute to high-grade serous ovarian cancer (HGSOC) recurrence. Stem phenotypes involving Wnt-β-catenin, aldehyde dehydrogenase activities, intrinsic platinum resistance, and tumorsphere formation are here ass...

journal_title：eLife

authors： Diaz Osterman CJ,Ozmadenci D,Kleinschmidt EG,Taylor KN,Barrie AM,Jiang S,Bean LM,Sulzmaier FJ,Jean C,Tancioni I,Anderson K,Uryu S,Cordasco EA,Li J,Chen XL,Fu G,Ojalill M,Rappu P,Heino J,Mark AM,Xu G,Fisch KM,K

更新日期：2019-09-03 00:00:00

abstract：:New studies examine how the different sub-structures in the cerebellum are organized to receive information during complex behavioral tasks. ...

journal_title：eLife

authors： Holloway AL,Lerner TN

更新日期：2019-11-14 00:00:00

abstract：:Trypanosome parasites are hiding in human skin, a discovery that may undermine efforts to eliminate sleeping sickness by 2020. ...

journal_title：eLife

authors： Casas-Sánchez A,Acosta-Serrano Á

更新日期：2016-10-14 00:00:00

abstract：:As the equipment needed to perform state-of-the-art research in many areas of biology becomes ever more expensive, Eve Marder worries that researchers in less wealthy institutions might be left behind. ...

journal_title：eLife

authors： Marder E

更新日期：2013-11-19 00:00:00

abstract：:Iron and heme play central roles in the production of red blood cells, but the underlying mechanisms remain incompletely understood. Heme-regulated eIF2α kinase (HRI) controls translation by phosphorylating eIF2α. Here, we investigate the global impact of iron, heme, and HRI on protein translation in vivo in murine pr...

journal_title：eLife

authors： Zhang S,Macias-Garcia A,Ulirsch JC,Velazquez J,Butty VL,Levine SS,Sankaran VG,Chen JJ

更新日期：2019-04-29 00:00:00

abstract：:Comparing the brains of related species faces the challenges of establishing homologies whilst accommodating evolutionary specializations. Here we propose a general framework for understanding similarities and differences between the brains of primates. The approach uses white matter blueprints of the whole cortex bas...

journal_title：eLife

authors： Mars RB,Sotiropoulos SN,Passingham RE,Sallet J,Verhagen L,Khrapitchev AA,Sibson N,Jbabdi S

更新日期：2018-05-11 00:00:00

abstract：:The mammalian auditory sensory epithelium has one of the most stereotyped cellular patterns known in vertebrates. Mechano-sensory hair cells are arranged in precise rows, with one row of inner and three rows of outer hair cells spanning the length of the spiral-shaped sensory epithelium. Aiding such precise cellular p...

journal_title：eLife

authors： Prajapati-DiNubila M,Benito-Gonzalez A,Golden EJ,Zhang S,Doetzlhofer A

更新日期：2019-06-12 00:00:00

abstract：:Experiments on flies suggest that a gain-of-function mechanism in a protein called CSPɑ contributes to the progressive brain disease CLN4. ...

journal_title：eLife

authors： Berryer MH,Kosmaczewski SG,Barrett LE

更新日期：2019-12-06 00:00:00

abstract：:Dopamine and striatal dysfunctions play a key role in the pathophysiology of Parkinson's disease (PD) and Dystonia, but our understanding of the changes in the discharge rate and pattern of striatal projection neurons (SPNs) remains limited. Here, we recorded and examined multi-unit signals from the striatum of PD and...

journal_title：eLife

authors： Valsky D,Heiman Grosberg S,Israel Z,Boraud T,Bergman H,Deffains M

更新日期：2020-08-19 00:00:00

abstract：:Circadian rhythms are biological oscillations that schedule daily changes in physiology. Outside the laboratory, circadian clocks do not generally free-run but are driven by daily cues whose timing varies with the seasons. The principles that determine how circadian clocks align to these external cycles are not well u...

journal_title：eLife

authors： Leypunskiy E,Lin J,Yoo H,Lee U,Dinner AR,Rust MJ

更新日期：2017-07-07 00:00:00

abstract：:Neurons form bona fide synapses with oligodendrocyte precursor cells (OPCs), but the circuit context of these neuron to OPC synapses remains incompletely understood. Using monosynaptically-restricted rabies virus tracing of OPC afferents, we identified extensive afferent synaptic inputs to OPCs residing in secondary m...

journal_title：eLife

authors： Mount CW,Yalçın B,Cunliffe-Koehler K,Sundaresh S,Monje M

更新日期：2019-10-18 00:00:00

abstract：:Modern neuroscience research often requires the coordination of multiple processes such as stimulus generation, real-time experimental control, as well as behavioral and neural measurements. The technical demands required to simultaneously manage these processes with high temporal fidelity is a barrier that limits the...

journal_title：eLife

authors： Kim B,Kenchappa SC,Sunkara A,Chang TY,Thompson L,Doudlah R,Rosenberg A

更新日期：2019-02-07 00:00:00

abstract：:Interaction between disease-microbiome associations and ageing has not been explored in detail. Here, using age/region-matched sub-sets, we analysed the gut microbiome differences across five major diseases in a multi-cohort dataset constituting more than 2500 individuals from 20 to 89 years old. We show that disease-...

journal_title：eLife

authors： Ghosh TS,Das M,Jeffery IB,O'Toole PW

更新日期：2020-03-11 00:00:00

abstract：:Using knowledge of the structure of the world to infer value is at the heart of model-based reasoning and relies on a circuit that includes the orbitofrontal cortex (OFC). Some accounts link this to the representation of biological significance or value by neurons in OFC, while other models focus on the representation...

journal_title：eLife

authors： Sadacca BF,Wied HM,Lopatina N,Saini GK,Nemirovsky D,Schoenbaum G

更新日期：2018-03-07 00:00:00

abstract：:Proteins can self-organize into spatial patterns via non-linear dynamic interactions on cellular membranes. Modelling and simulations have shown that small GTPases can generate patterns by coupling guanine nucleotide exchange factors (GEF) to effectors, generating a positive feedback of GTPase activation and membrane ...

journal_title：eLife

authors： Cezanne A,Lauer J,Solomatina A,Sbalzarini IF,Zerial M

更新日期：2020-06-08 00:00:00

abstract：:Accurate protein localization is crucial to generate and maintain organization in all cells. Achieving accuracy is challenging, as the molecular signals that dictate a protein's cellular destination are often promiscuous. A salient example is the targeting of an essential class of tail-anchored (TA) proteins, whose so...

journal_title：eLife

authors： Rao M,Okreglak V,Chio US,Cho H,Walter P,Shan SO

更新日期：2016-12-07 00:00:00

abstract：:The Cdc45/Mcm2-7/GINS (CMG) helicase separates DNA strands during replication in eukaryotes. How the CMG is assembled and engages DNA substrates remains unclear. Using electron microscopy, we have determined the structure of the CMG in the presence of ATPγS and a DNA duplex bearing a 3' single-stranded tail. The struc...

journal_title：eLife

authors： Costa A,Renault L,Swuec P,Petojevic T,Pesavento JJ,Ilves I,MacLellan-Gibson K,Fleck RA,Botchan MR,Berger JM

更新日期：2014-08-12 00:00:00