Gene autoregulation by 3' UTR-derived bacterial small RNAs.

abstract：:How different helicase families with a conserved catalytic 'helicase core' evolved to function on varied RNA and DNA substrates by diverse mechanisms remains unclear. In this study, we used Mss116, a yeast DEAD-box protein that utilizes ATP to locally unwind dsRNA, to investigate helicase specificity and mechanism. Ou...

journal_title：eLife

authors： Mallam AL,Sidote DJ,Lambowitz AM

更新日期：2014-12-12 00:00:00

abstract：:Unfavorable redox conditions in the endoplasmic reticulum (ER) can decrease the capacity for protein secretion, altering vital cell functions. While systems to manage reductive stress are well-established, how cells cope with an overly oxidizing ER remains largely undefined. In previous work (Wang et al., 2014), we de...

journal_title：eLife

authors： Siegenthaler KD,Pareja KA,Wang J,Sevier CS

更新日期：2017-03-03 00:00:00

abstract：:Cell division is essential to expand, shape, and replenish epithelia. In the adult small intestine, cells from a common progenitor intermix with other lineages, whereas cell progeny in many other epithelia form contiguous patches. The mechanisms that generate these distinct patterns of progeny are poorly understood. U...

journal_title：eLife

authors： McKinley KL,Stuurman N,Royer LA,Schartner C,Castillo-Azofeifa D,Delling M,Klein OD,Vale RD

更新日期：2018-06-13 00:00:00

abstract：:Clustering of receptors associated with immunoreceptor tyrosine-based activation motifs (ITAMs) initiates the macrophage antimicrobial response. ITAM receptors engage Src-family tyrosine kinases (SFKs) to initiate phagocytosis and macrophage activation. Macrophages also encounter nonpathogenic molecules that cluster r...

journal_title：eLife

authors： Freedman TS,Tan YX,Skrzypczynska KM,Manz BN,Sjaastad FV,Goodridge HS,Lowell CA,Weiss A

更新日期：2015-10-30 00:00:00

abstract：:Cells respond to changes in their environment through signaling networks that modulate cytoskeleton and membrane organization to coordinate cell-cycle progression, polarized cell growth and multicellular development. Here, we define a novel regulatory mechanism by which the motor activity and function of the fission y...

journal_title：eLife

authors： Baker K,Gyamfi IA,Mashanov GI,Molloy JE,Geeves MA,Mulvihill DP

更新日期：2019-09-30 00:00:00

abstract：:Early life adversity (ELA) is associated with increased risk for stress-related disorders later in life. The link between ELA and risk for psychopathology is well established but the developmental mechanisms remain unclear. Using a mouse model of resource insecurity, limited bedding (LB), we tested the effects of LB o...

journal_title：eLife

authors： Manzano Nieves G,Bravo M,Baskoylu S,Bath KG

更新日期：2020-07-21 00:00:00

abstract：:In 2015, as part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Kandela et al., 2015) that described how we intended to replicate selected experiments from the paper "BET bromodomain inhibition as a therapeutic strategy to target c-Myc" (Delmore et al., 2011). Here we report the resu...

journal_title：eLife

authors： Aird F,Kandela I,Mantis C,Reproducibility Project: Cancer Biology.

更新日期：2017-01-19 00:00:00

abstract：:The medial subnucleus of the amygdala (MeA) plays a central role in processing sensory cues required for innate behaviors. However, whether there is a link between developmental programs and the emergence of inborn behaviors remains unknown. Our previous studies revealed that the telencephalic preoptic area (POA) embr...

journal_title：eLife

authors： Lischinsky JE,Sokolowski K,Li P,Esumi S,Kamal Y,Goodrich M,Oboti L,Hammond TR,Krishnamoorthy M,Feldman D,Huntsman M,Liu J,Corbin JG

更新日期：2017-03-13 00:00:00

abstract：:Meiotic drive is the preferential transmission of a particular allele during sexual reproduction. The phenomenon is observed as spore killing in multiple fungi. In natural populations of Podospora anserina, seven spore killer types (Psks) have been identified through classical genetic analyses. Here we show that the S...

journal_title：eLife

authors： Vogan AA,Ament-Velásquez SL,Granger-Farbos A,Svedberg J,Bastiaans E,Debets AJ,Coustou V,Yvanne H,Clavé C,Saupe SJ,Johannesson H

更新日期：2019-07-26 00:00:00

abstract：:Cerebral cortical architecture at birth encodes regionally differential dendritic arborization and synaptic formation. It underlies behavioral emergence of 2-year-olds. Brain changes in 0-2 years are most dynamic across the lifespan. Effective prediction of future behavior with brain microstructure at birth will revea...

journal_title：eLife

authors： Ouyang M,Peng Q,Jeon T,Heyne R,Chalak L,Huang H

更新日期：2020-12-22 00:00:00

abstract：:MyD88 is the main adaptor molecule for TLR and IL-1R family members. Here, we demonstrated that T-cell intrinsic MyD88 signaling is required for proliferation, protection from apoptosis and expression of activation/memory genes during infection with the intracellular parasite Trypanosoma cruzi, as evidenced by transcr...

journal_title：eLife

authors： Oliveira AC,Gomes-Neto JF,Barbosa CD,Granato A,Reis BS,Santos BM,Fucs R,Canto FB,Nakaya HI,Nóbrega A,Bellio M

更新日期：2017-09-12 00:00:00

abstract：:Recent advances in single-cell transcriptomics techniques have opened the door to the study of gene regulatory networks (GRNs) at the single-cell level. Here, we studied the GRNs controlling the emergence of hematopoietic stem and progenitor cells from mouse embryonic endothelium using a combination of single-cell tra...

journal_title：eLife

authors： Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

更新日期：2018-03-20 00:00:00

abstract：:Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissue...

journal_title：eLife

authors： Nagarkar-Jaiswal S,Lee PT,Campbell ME,Chen K,Anguiano-Zarate S,Gutierrez MC,Busby T,Lin WW,He Y,Schulze KL,Booth BW,Evans-Holm M,Venken KJ,Levis RW,Spradling AC,Hoskins RA,Bellen HJ

更新日期：2015-03-31 00:00:00

abstract：:Eukaryotic kinetochores connect spindlemicrotubules to chromosomal centromeres. A group of proteins called the Ctf19 complex (Ctf19c) in yeast and the constitutive centromere associated network (CCAN) in other organisms creates the foundation of a kinetochore. The Ctf19c/CCAN influences the timing of kinetochore assem...

journal_title：eLife

authors： Hinshaw SM,Harrison SC

更新日期：2019-02-14 00:00:00

abstract：:The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the...

journal_title：eLife

authors： Evans B,Griner E,Reproducibility Project: Cancer Biology.

更新日期：2015-07-31 00:00:00

abstract：:Understanding how fungi specialize on their plant host is crucial for developing sustainable disease control. A traditional, centuries-old rice agro-system of the Yuanyang terraces was used as a model to show that virulence effectors of the rice blast fungus Magnaporthe oryzaeh play a key role in its specialization on...

journal_title：eLife

authors： Liao J,Huang H,Meusnier I,Adreit H,Ducasse A,Bonnot F,Pan L,He X,Kroj T,Fournier E,Tharreau D,Gladieux P,Morel JB

更新日期：2016-12-23 00:00:00

abstract：:The clathrin adaptor complex AP2 is thought to be an obligate heterotetramer. We identify null mutations in the α subunit of AP2 in the nematode Caenorhabditis elegans. α-adaptin mutants are viable and the remaining μ2/β hemicomplex retains some function. Conversely, in μ2 mutants, the alpha/sigma2 hemicomplex is loca...

journal_title：eLife

authors： Gu M,Liu Q,Watanabe S,Sun L,Hollopeter G,Grant BD,Jorgensen EM

更新日期：2013-03-05 00:00:00

abstract：:Signaling molecules activate distinct patterns of gene expression to coordinate embryogenesis, but how spatiotemporal expression diversity is generated is an open question. In zebrafish, a BMP signaling gradient patterns the dorsal-ventral axis. We systematically identified target genes responding to BMP and found tha...

journal_title：eLife

authors： Rogers KW,ElGamacy M,Jordan BM,Müller P

更新日期：2020-11-11 00:00:00

abstract：:Progression through the mitotic cell cycle requires periodic regulation of gene function at the levels of transcription, translation, protein-protein interactions, post-translational modification and degradation. However, the role of alternative splicing (AS) in the temporal control of cell cycle is not well understoo...

journal_title：eLife

authors： Dominguez D,Tsai YH,Weatheritt R,Wang Y,Blencowe BJ,Wang Z

更新日期：2016-03-25 00:00:00

abstract：:Perforin-2 (MPEG1) is a pore-forming, antibacterial protein with broad-spectrum activity. Perforin-2 is expressed constitutively in phagocytes and inducibly in parenchymal, tissue-forming cells. In vitro, Perforin-2 prevents the intracellular replication and proliferation of bacterial pathogens in these cells. Perfori...

journal_title：eLife

authors： McCormack RM,de Armas LR,Shiratsuchi M,Fiorentino DG,Olsson ML,Lichtenheld MG,Morales A,Lyapichev K,Gonzalez LE,Strbo N,Sukumar N,Stojadinovic O,Plano GV,Munson GP,Tomic-Canic M,Kirsner RS,Russell DG,Podack ER

更新日期：2015-09-24 00:00:00

abstract：:The voltage-gated K+ channel Kv2.1 serves a major structural role in the soma and proximal dendrites of mammalian brain neurons, tethering the plasma membrane (PM) to endoplasmic reticulum (ER). Although Kv2.1 clustering at neuronal ER-PM junctions (EPJs) is tightly regulated and highly conserved, its function remains...

journal_title：eLife

authors： Vierra NC,Kirmiz M,van der List D,Santana LF,Trimmer JS

更新日期：2019-10-30 00:00:00

abstract：:Serotonin (5-HT) is associated with mood and motivation but the function of endogenous 5-HT remains controversial. Here, we studied the impact of phasic optogenetic activation of 5-HT neurons in mice over time scales from seconds to weeks. We found that activating dorsal raphe nucleus (DRN) 5-HT neurons induced a stro...

journal_title：eLife

authors： Correia PA,Lottem E,Banerjee D,Machado AS,Carey MR,Mainen ZF

更新日期：2017-02-14 00:00:00

abstract：:Studies of cell fate focus on specification, but little is known about maintenance of the differentiated state. In this study, we find that the mouse tendon cell fate requires continuous maintenance in vivo and identify an essential role for TGFβ signaling in maintenance of the tendon cell fate. To examine the role of...

journal_title：eLife

authors： Tan GK,Pryce BA,Stabio A,Brigande JV,Wang C,Xia Z,Tufa SF,Keene DR,Schweitzer R

更新日期：2020-01-21 00:00:00

abstract：:Glutaminase (GLS) isoenzymes GLS1 and GLS2 are key enzymes for glutamine metabolism. Interestingly, GLS1 and GLS2 display contrasting functions in tumorigenesis with elusive mechanism; GLS1 promotes tumorigenesis, whereas GLS2 exhibits a tumor-suppressive function. In this study, we found that GLS2 but not GLS1 binds ...

journal_title：eLife

authors： Zhang C,Liu J,Zhao Y,Yue X,Zhu Y,Wang X,Wu H,Blanco F,Li S,Bhanot G,Haffty BG,Hu W,Feng Z

更新日期：2016-01-11 00:00:00

abstract：:Faithful chromosome segregation in all eukaryotes relies on centromeres, the chromosomal sites that recruit kinetochore proteins and mediate spindle attachment during cell division. The centromeric histone H3 variant, CenH3, is the defining chromatin component of centromeres in most eukaryotes, including animals, fung...

journal_title：eLife

authors： Drinnenberg IA,deYoung D,Henikoff S,Malik HS

更新日期：2014-09-23 00:00:00

abstract：:The incubation period for typhoid, polio, measles, leukemia and many other diseases follows a right-skewed, approximately lognormal distribution. Although this pattern was discovered more than sixty years ago, it remains an open question to explain its ubiquity. Here, we propose an explanation based on evolutionary dy...

journal_title：eLife

authors： Ottino-Loffler B,Scott JG,Strogatz SH

更新日期：2017-12-21 00:00:00

abstract：:Tuberculosis disproportionately affects the Canadian Inuit. To address this, it is imperative we understand transmission dynamics in this population. We investigate whether 'deep' sequencing can provide additional resolution compared to standard sequencing, using a well-characterized outbreak from the Arctic (2011-201...

journal_title：eLife

authors： Lee RS,Proulx JF,McIntosh F,Behr MA,Hanage WP

更新日期：2020-02-04 00:00:00

abstract：:Cell surface reception of Sonic hedgehog (Shh) must ensure that the graded morphogenic signal is interpreted accordingly in neighboring cells to specify tissue patterns during development. Here, we report endocytic sorting signals for the receptor Patched1 (Ptch1), comprising two 'PPXY' motifs, that direct it to degra...

journal_title：eLife

authors： Yue S,Tang LY,Tang Y,Tang Y,Shen QH,Ding J,Chen Y,Zhang Z,Yu TT,Zhang YE,Cheng SY

更新日期：2014-06-12 00:00:00

abstract：:How producers of public goods persist in microbial communities is a major question in evolutionary biology. Cooperation is evolutionarily unstable, since cheating strains can reproduce quicker and take over. Spatial structure has been shown to be a robust mechanism for the evolution of cooperation. Here we study how s...

journal_title：eLife

authors： Uppal G,Vural DC

更新日期：2018-05-22 00:00:00

abstract：:Pervasive expression of circular RNA is a recently discovered feature of eukaryotic gene expression programs, yet its function remains largely unknown. The presumed biogenesis of these RNAs involves a non-canonical 'backsplicing' event. Recent studies in mammalian cell culture posit that backsplicing is facilitated by...

journal_title：eLife

authors： Barrett SP,Wang PL,Salzman J

更新日期：2015-06-09 00:00:00