The leak channel NALCN controls tonic firing and glycolytic sensitivity of substantia nigra pars reticulata neurons.

abstract：:Mammalian two-pore-channels (TPC1, 2; TPCN1, TPCN2) are ubiquitously- expressed, PI(3,5)P2-activated, Na+-selective channels in the endosomes and lysosomes that regulate luminal pH homeostasis, membrane trafficking, and Ebola viral infection. Whereas the channel activity of TPC1 is strongly dependent on membrane volta...

journal_title：eLife

authors： Zhang X,Chen W,Li P,Calvo R,Southall N,Hu X,Bryant-Genevier M,Feng X,Geng Q,Gao C,Yang M,Tang K,Ferrer M,Marugan JJ,Xu H

更新日期：2019-12-11 00:00:00

abstract：:Tissue mechanics is important for development; however, the spatio-temporal dynamics of in vivo tissue stiffness is still poorly understood. We here developed tiv-AFM, combining time-lapse in vivo atomic force microscopy with upright fluorescence imaging of embryonic tissue, to show that during development local tissu...

journal_title：eLife

authors： Thompson AJ,Pillai EK,Dimov IB,Foster SK,Holt CE,Franze K

更新日期：2019-01-15 00:00:00

abstract：:Optogenetic approaches are leading to a better understanding of the forces that determine the plane of cell division. ...

journal_title：eLife

authors： Serra-Marques A,Dumont S

更新日期：2018-07-09 00:00:00

abstract：:AKT is implicated in neurological disorders. AKT has three isoforms, AKT1/AKT2/AKT3, with brain cell type-specific expression that may differentially influence behavior. Therefore, we examined single Akt isoform, conditional brain-specific Akt1, and double Akt1/3 mutant mice in behaviors relevant to neuropsychiatric d...

journal_title：eLife

authors： Wong H,Levenga J,LaPlante L,Keller B,Cooper-Sansone A,Borski C,Milstead R,Ehringer M,Hoeffer C

更新日期：2020-12-16 00:00:00

abstract：:Cell-type-specific 3D organization of the genome is unrecognizable during mitosis. It remains unclear how essential positional information is transmitted through cell division such that a daughter cell recapitulates the spatial genome organization of the parent. Lamina-associated domains (LADs) are regions of repressi...

journal_title：eLife

authors： Poleshko A,Smith CL,Nguyen SC,Sivaramakrishnan P,Wong KG,Murray JI,Lakadamyali M,Joyce EF,Jain R,Epstein JA

更新日期：2019-10-01 00:00:00

abstract：:The Sae2/CtIP protein is required for efficient processing of DNA double-strand breaks that initiate homologous recombination in eukaryotic cells. Sae2/CtIP is also important for survival of single-stranded Top1-induced lesions and CtIP is known to associate directly with transcription-associated complexes in mammalia...

journal_title：eLife

authors： Makharashvili N,Arora S,Yin Y,Fu Q,Wen X,Lee JH,Kao CH,Leung JW,Miller KM,Paull TT

更新日期：2018-12-07 00:00:00

abstract：:Msp1 is a conserved AAA ATPase in budding yeast localized to mitochondria where it prevents accumulation of mistargeted tail-anchored (TA) proteins, including the peroxisomal TA protein Pex15. Msp1 also resides on peroxisomes but it remains unknown how native TA proteins on mitochondria and peroxisomes evade Msp1 surv...

journal_title：eLife

authors： Weir NR,Kamber RA,Martenson JS,Denic V

更新日期：2017-09-14 00:00:00

abstract：:The insular cortex (IC) plays key roles in emotional and regulatory brain functions and is affected across psychiatric diseases. However, the brain-wide connections of the mouse IC have not been comprehensively mapped. Here, we traced the whole-brain inputs and outputs of the mouse IC across its rostro-caudal extent. ...

journal_title：eLife

authors： Gehrlach DA,Weiand C,Gaitanos TN,Cho E,Klein AS,Hennrich AA,Conzelmann KK,Gogolla N

更新日期：2020-09-17 00:00:00

abstract：:The ellipsoid body (EB) in the Drosophila brain is a central complex (CX) substructure that harbors circumferentially laminated ring (R) neuron axons and mediates multifaceted sensory integration and motor coordination functions. However, what regulates R axon lamination and how lamination affects R neuron function re...

journal_title：eLife

authors： Xie X,Tabuchi M,Brown MP,Mitchell SP,Wu MN,Kolodkin AL

更新日期：2017-06-20 00:00:00

abstract：:How does the skeleton detect and adapt to changes in the mechanical load it has to carry? ...

journal_title：eLife

authors： Haelterman N,Lim J

更新日期：2019-10-07 00:00:00

abstract：:Anterograde transport of late endosomes or lysosomes (LE/Lys) is crucial for proper axon growth. However, the role of energetic nutrients has been poorly explored. Malonyl-CoA is a precursor of fatty acids, and its intracellular levels highly fluctuate depending on glucose availability or the energy sensor AMP-activat...

journal_title：eLife

authors： Palomo-Guerrero M,Fadó R,Casas M,Pérez-Montero M,Baena M,Helmer PO,Domínguez JL,Roig A,Serra D,Hayen H,Stenmark H,Raiborg C,Casals N

更新日期：2019-12-23 00:00:00

abstract：:The NuRD complex is a multi-protein transcriptional corepressor that couples histone deacetylase and ATP-dependent chromatin remodelling activities. The complex regulates the higher-order structure of chromatin, and has important roles in the regulation of gene expression, DNA damage repair and cell differentiation. H...

journal_title：eLife

authors： Millard CJ,Varma N,Saleh A,Morris K,Watson PJ,Bottrill AR,Fairall L,Smith CJ,Schwabe JW

更新日期：2016-04-21 00:00:00

abstract：:Uropathogenic E. coli (UPEC), which cause urinary tract infections (UTI), utilize type 1 pili, a chaperone usher pathway (CUP) pilus, to cause UTI and colonize the gut. The pilus rod, comprised of repeating FimA subunits, provides a structural scaffold for displaying the tip adhesin, FimH. We solved the 4.2 Å resoluti...

journal_title：eLife

authors： Spaulding CN,Schreiber HL 4th,Zheng W,Dodson KW,Hazen JE,Conover MS,Wang F,Svenmarker P,Luna-Rico A,Francetic O,Andersson M,Hultgren S,Egelman EH

更新日期：2018-01-18 00:00:00

abstract：:The diaphragm muscle is essential for breathing in mammals. Its asymmetric elevation during contraction correlates with morphological features suggestive of inherent left-right (L/R) asymmetry. Whether this asymmetry is due to L versus R differences in the muscle or in the phrenic nerve activity is unknown. Here, we h...

journal_title：eLife

authors： Charoy C,Dinvaut S,Chaix Y,Morlé L,Sanyas I,Bozon M,Kindbeiter K,Durand B,Skidmore JM,De Groef L,Seki M,Moons L,Ruhrberg C,Martin JF,Martin DM,Falk J,Castellani V

更新日期：2017-06-22 00:00:00

abstract：:Mapping polyclonal serum responses is critical to rational vaccine design. However, most high-resolution mapping approaches involve isolating and characterizing individual antibodies, which incompletely defines the polyclonal response. Here we use two complementary approaches to directly map the specificities of the n...

journal_title：eLife

authors： Dingens AS,Pratap P,Malone KD,Hilton SK,Ketas T,Cottrell CA,Overbaugh JM,Moore JP,Klasse PJ,Ward AB,Bloom JD

更新日期：2021-01-13 00:00:00

abstract：:DNA is a remarkably precise medium for copying and storing biological information. This high fidelity results from the action of hundreds of genes involved in replication, proofreading, and damage repair. Evolutionary theory suggests that in such a system, selection has limited ability to remove genetic variants that ...

journal_title：eLife

authors： Harris K,Pritchard JK

更新日期：2017-04-25 00:00:00

abstract：:Transparent reporting is essential for the critical evaluation of studies. However, the reporting of statistical methods for studies in the biomedical sciences is often limited. This systematic review examines the quality of reporting for two statistical tests, t-tests and ANOVA, for papers published in a selection of...

journal_title：eLife

authors： Weissgerber TL,Garcia-Valencia O,Garovic VD,Milic NM,Winham SJ

更新日期：2018-12-21 00:00:00

abstract：:Synaptic adhesion molecules regulate synapse development and function. However, whether and how presynaptic adhesion molecules regulate postsynaptic NMDAR function remains largely unclear. Presynaptic LAR family receptor tyrosine phosphatases (LAR-RPTPs) regulate synapse development through mechanisms that include tra...

journal_title：eLife

authors： Kim K,Shin W,Kang M,Lee S,Kim D,Kang R,Jung Y,Cho Y,Yang E,Kim H,Bae YC,Kim E

更新日期：2020-03-06 00:00:00

abstract：:A polymorphism in the autophagy gene Atg16l1 is associated with susceptibility to inflammatory bowel disease (IBD); however, it remains unclear how autophagy contributes to intestinal immune homeostasis. Here, we demonstrate that autophagy is essential for maintenance of balanced CD4(+) T cell responses in the intesti...

journal_title：eLife

authors： Kabat AM,Harrison OJ,Riffelmacher T,Moghaddam AE,Pearson CF,Laing A,Abeler-Dörner L,Forman SP,Grencis RK,Sattentau Q,Simon AK,Pott J,Maloy KJ

更新日期：2016-02-24 00:00:00

abstract：:While traditional microbiological freshwater tests focus on the detection of specific bacterial indicator species, including pathogens, direct tracing of all aquatic DNA through metagenomics poses a profound alternative. Yet, in situ metagenomic water surveys face substantial challenges in cost and logistics. Here, we...

journal_title：eLife

authors： Urban L,Holzer A,Baronas JJ,Hall MB,Braeuninger-Weimer P,Scherm MJ,Kunz DJ,Perera SN,Martin-Herranz DE,Tipper ET,Salter SJ,Stammnitz MR

更新日期：2021-01-19 00:00:00

abstract：:Bacterial flagella mediate host-microbe interactions through tissue tropism during colonization, as well as by activating immune responses. The flagellar shaft of some bacteria, including several human pathogens, is encased in a membranous sheath of unknown function. While it has been hypothesized that the sheath may ...

journal_title：eLife

authors： Brennan CA,Hunt JR,Kremer N,Krasity BC,Apicella MA,McFall-Ngai MJ,Ruby EG

更新日期：2014-03-04 00:00:00

abstract：:Here, we describe the case of a COVID-19 patient who developed recurring ventilator-associated pneumonia caused by Pseudomonas aeruginosa that acquired increasing levels of antimicrobial resistance (AMR) in response to treatment. Metagenomic analysis revealed the AMR genotype, while immunological analysis revealed mas...

journal_title：eLife

authors： Gregorova M,Morse D,Brignoli T,Steventon J,Hamilton F,Albur M,Arnold D,Thomas M,Halliday A,Baum H,Rice C,Avison MB,Davidson AD,Santopaolo M,Oliver E,Goenka A,Finn A,Wooldridge L,Amulic B,Boyton RJ,Altmann DM,But

更新日期：2020-12-17 00:00:00

abstract：:We are writing to comment on the recent study by Olesen et al., 2018 on antibiotic use and antibiotic resistance. ...

journal_title：eLife

authors： Pouwels KB,Butler CC,Robotham JV

更新日期：2019-05-03 00:00:00

abstract：:The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the...

journal_title：eLife

authors： Phelps M,Coss C,Wang H,Cook M,Reproducibility Project: Cancer Biology.,Reproducibility Project Cancer Biology.

更新日期：2016-03-01 00:00:00

abstract：:Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmenta...

journal_title：eLife

authors： Lleras Forero L,Narayanan R,Huitema LF,VanBergen M,Apschner A,Peterson-Maduro J,Logister I,Valentin G,Morelli LG,Oates AC,Schulte-Merker S

更新日期：2018-04-06 00:00:00

abstract：:In response to cell death signals, an active apoptosome is assembled from Apaf-1 and procaspase-9 (pc-9). Here we report a near atomic structure of the active human apoptosome determined by cryo-electron microscopy. The resulting model gives insights into cytochrome c binding, nucleotide exchange and conformational ch...

journal_title：eLife

authors： Cheng TC,Hong C,Akey IV,Yuan S,Akey CW

更新日期：2016-10-04 00:00:00

abstract：:A major challenge in neuroscience is to longitudinally monitor whole brain activity across multiple spatial scales in the same animal. Functional UltraSound (fUS) is an emerging technology that offers images of cerebral blood volume over large brain portions. Here we show for the first time its capability to resolve t...

journal_title：eLife

authors： Bimbard C,Demene C,Girard C,Radtke-Schuller S,Shamma S,Tanter M,Boubenec Y

更新日期：2018-06-28 00:00:00

abstract：:Secretory (S) Immunoglobulin (Ig) A is the predominant mucosal antibody, which binds pathogens and commensal microbes. SIgA is a polymeric antibody, typically containing two copies of IgA that assemble with one joining-chain (JC) to form dimeric (d) IgA that is bound by the polymeric Ig-receptor ectodomain, called sec...

journal_title：eLife

authors： Kumar Bharathkar S,Parker BW,Malyutin AG,Haloi N,Huey-Tubman KE,Tajkhorshid E,Stadtmueller BM

更新日期：2020-10-27 00:00:00

abstract：:TFIID-a complex of TATA-binding protein (TBP) and TBP-associated factors (TAFs)-is a central component of the Pol II promoter recognition apparatus. Recent studies have revealed significant downregulation of TFIID subunits in terminally differentiated myocytes, hepatocytes and adipocytes. Here, we report that TBP prot...

journal_title：eLife

authors： Li L,Martinez SS,Hu W,Liu Z,Tjian R

更新日期：2015-09-22 00:00:00

abstract：:Cytoplasmic dyneins are motor proteins in the AAA+ superfamily that transport cellular cargos toward microtubule minus-ends. Recently, ciliobrevins were reported as selective cell-permeable inhibitors of cytoplasmic dyneins. As is often true for first-in-class inhibitors, the use of ciliobrevins has in part been limit...

journal_title：eLife

authors： Steinman JB,Santarossa CC,Miller RM,Yu LS,Serpinskaya AS,Furukawa H,Morimoto S,Tanaka Y,Nishitani M,Asano M,Zalyte R,Ondrus AE,Johnson AG,Ye F,Nachury MV,Fukase Y,Aso K,Foley MA,Gelfand VI,Chen JK,Carter AP,Kapo

更新日期：2017-05-19 00:00:00