Comment on 'The distribution of antibiotic use and its association with antibiotic resistance'.

abstract：:Disturbance from whale-watching can cause significant behavioural changes with fitness consequences for targeted whale populations. However, the sensory stimuli triggering these responses are unknown, preventing effective mitigation. Here, we test the hypothesis that vessel noise level is a driver of disturbance, usin...

journal_title：eLife

authors： Sprogis KR,Videsen S,Madsen PT

更新日期：2020-06-16 00:00:00

abstract：:A strain of budding yeast that contains one large chromosome reveals how the telomere capping complex CST maintains linear but not circular chromosomes. ...

journal_title：eLife

authors： Nugent C,Sugimoto K

更新日期：2020-08-05 00:00:00

abstract：:Macrophages derive from multiple sources of hematopoietic progenitors. Most macrophages require colony-stimulating factor 1 receptor (CSF1R), but some macrophages persist in the absence of CSF1R. Here, we analyzed mpeg1:GFP-expressing macrophages in csf1r-deficient zebrafish and report that embryonic macrophages emerg...

journal_title：eLife

authors： Kuil LE,Oosterhof N,Ferrero G,Mikulášová T,Hason M,Dekker J,Rovira M,van der Linde HC,van Strien PM,de Pater E,Schaaf G,Bindels EM,Wittamer V,van Ham TJ

更新日期：2020-05-05 00:00:00

abstract：:Cytoplasmic dyneins are motor proteins in the AAA+ superfamily that transport cellular cargos toward microtubule minus-ends. Recently, ciliobrevins were reported as selective cell-permeable inhibitors of cytoplasmic dyneins. As is often true for first-in-class inhibitors, the use of ciliobrevins has in part been limit...

journal_title：eLife

authors： Steinman JB,Santarossa CC,Miller RM,Yu LS,Serpinskaya AS,Furukawa H,Morimoto S,Tanaka Y,Nishitani M,Asano M,Zalyte R,Ondrus AE,Johnson AG,Ye F,Nachury MV,Fukase Y,Aso K,Foley MA,Gelfand VI,Chen JK,Carter AP,Kapo

更新日期：2017-05-19 00:00:00

abstract：:Meiotic crossovers result from homology-directed repair of DNA double-strand breaks (DSBs). Unlike yeast and plants, where DSBs are generated near gene promoters, in many vertebrates DSBs are enriched at hotspots determined by the DNA binding activity of the rapidly evolving zinc finger array of PRDM9 (PR domain zinc ...

journal_title：eLife

authors： Mahgoub M,Paiano J,Bruno M,Wu W,Pathuri S,Zhang X,Ralls S,Cheng X,Nussenzweig A,Macfarlan TS

更新日期：2020-04-30 00:00:00

abstract：:Synaptic adhesion molecules regulate synapse development and function. However, whether and how presynaptic adhesion molecules regulate postsynaptic NMDAR function remains largely unclear. Presynaptic LAR family receptor tyrosine phosphatases (LAR-RPTPs) regulate synapse development through mechanisms that include tra...

journal_title：eLife

authors： Kim K,Shin W,Kang M,Lee S,Kim D,Kang R,Jung Y,Cho Y,Yang E,Kim H,Bae YC,Kim E

更新日期：2020-03-06 00:00:00

abstract：:Myosins play essential roles in the development and function of auditory organs and multiple myosin genes are associated with hereditary forms of deafness. Using a forward genetic screen in Drosophila, we identified an E3 ligase, Ubr3, as an essential gene for auditory organ development. Ubr3 negatively regulates the ...

journal_title：eLife

authors： Li T,Giagtzoglou N,Eberl DF,Jaiswal SN,Cai T,Godt D,Groves AK,Bellen HJ

更新日期：2016-06-22 00:00:00

abstract：:Across species, sleep in young animals is critical for normal brain maturation. The molecular determinants of early life sleep remain unknown. Through an RNAi-based screen, we identified a gene, pdm3, required for sleep maturation in Drosophila. Pdm3, a transcription factor, coordinates an early developmental program ...

journal_title：eLife

authors： Chakravarti Dilley L,Szuperak M,Gong NN,Williams CE,Saldana RL,Garbe DS,Syed MH,Jain R,Kayser MS

更新日期：2020-03-23 00:00:00

abstract：:The number of de novo mutations (DNMs) found in an offspring's genome increases with both paternal and maternal age. But does the rate of mutation accumulation in human gametes differ across families? Using sequencing data from 33 large, three-generation CEPH families, we observed significant variability in parental a...

journal_title：eLife

authors： Sasani TA,Pedersen BS,Gao Z,Baird L,Przeworski M,Jorde LB,Quinlan AR

更新日期：2019-09-24 00:00:00

abstract：:A key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a chromatin donor template instead of a naked DNA donor template. The use of chromatin, which is the natural form of DNA in the nucleus, ...

journal_title：eLife

authors： Cruz-Becerra G,Kadonaga JT

更新日期：2020-04-28 00:00:00

abstract：:The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucleosome, constrains t...

journal_title：eLife

authors： Isaac RS,Jiang F,Doudna JA,Lim WA,Narlikar GJ,Almeida R

更新日期：2016-04-28 00:00:00

abstract：:Stem cells are maintained in a specialized microenvironment called niche but the nature of stem cell niche remains poorly defined in many systems. Here we demonstrate that intestinal epithelium-derived BMP serves as a niche signal for intestinal stem cell (ISC) self-renewal in Drosophila adult midgut. We find that BMP...

journal_title：eLife

authors： Tian A,Jiang J

更新日期：2014-03-11 00:00:00

abstract：:Trypanosome parasites are hiding in human skin, a discovery that may undermine efforts to eliminate sleeping sickness by 2020. ...

journal_title：eLife

authors： Casas-Sánchez A,Acosta-Serrano Á

更新日期：2016-10-14 00:00:00

abstract：:Signaling molecules activate distinct patterns of gene expression to coordinate embryogenesis, but how spatiotemporal expression diversity is generated is an open question. In zebrafish, a BMP signaling gradient patterns the dorsal-ventral axis. We systematically identified target genes responding to BMP and found tha...

journal_title：eLife

authors： Rogers KW,ElGamacy M,Jordan BM,Müller P

更新日期：2020-11-11 00:00:00

abstract：:TMEM16A is a ligand-gated anion channel that is activated by intracellular Ca2+. This channel comprises two independent pores and closely apposed Ca2+ binding sites that are contained within each subunit of a homodimeric protein. Previously we characterized the influence of positively charged pore-lining residues on a...

journal_title：eLife

authors： Lam AK,Dutzler R

更新日期：2018-10-12 00:00:00

abstract：:LAMP1 and LAMP2 proteins are highly abundant, ubiquitous, mammalian proteins that line the lysosome limiting membrane, and protect it from lysosomal hydrolase action. LAMP2 deficiency causes Danon's disease, an X-linked hypertrophic cardiomyopathy. LAMP2 is needed for chaperone-mediated autophagy, and its expression i...

journal_title：eLife

authors： Li J,Pfeffer SR

更新日期：2016-09-24 00:00:00

abstract：:Human lung adenocarcinoma exhibits a propensity for de-differentiation, complicating diagnosis and treatment, and predicting poorer patient survival. In genetically engineered mouse models of lung cancer, expression of the BRAFV600E oncoprotein kinase initiates the growth of benign tumors retaining characteristics of ...

journal_title：eLife

authors： van Veen JE,Scherzer M,Boshuizen J,Chu M,Liu A,Landman A,Green S,Trejo C,McMahon M

更新日期：2019-08-27 00:00:00

abstract：:Experience-dependent modifications of synaptic connections are thought to change patterns of network activities and stimulus tuning with learning. However, only a few studies explored how synaptic plasticity shapes the response dynamics of cortical circuits. Here, we investigated the mechanism underlying sharpening of...

journal_title：eLife

authors： Lim S

更新日期：2019-08-08 00:00:00

abstract：: ...

journal_title：eLife

authors： MacKay CE,Leo MD,Fernández-Peña C,Hasan R,Yin W,Mata-Daboin A,Bulley S,Gammons J,Mancarella S,Jaggar JH

更新日期：2020-06-30 00:00:00

abstract：:The nematode Caenorhabditis elegans possesses a simple embryonic nervous system with few enough neurons that the growth of each cell could be followed to provide a systems-level view of development. However, studies of single cell development have largely been conducted in fixed or pre-twitching live embryos, because ...

journal_title：eLife

authors： Christensen RP,Bokinsky A,Santella A,Wu Y,Marquina-Solis J,Guo M,Kovacevic I,Kumar A,Winter PW,Tashakkori N,McCreedy E,Liu H,McAuliffe M,Mohler W,Colón-Ramos DA,Bao Z,Shroff H

更新日期：2015-12-03 00:00:00

abstract：:Researchers have identified two genes-follistatin and activin-as having an important role in the ability of certain flatworms to identify wounds that require the production of new tissue. ...

journal_title：eLife

authors： Sánchez Alvarado A

更新日期：2013-09-10 00:00:00

abstract：:Binary fission has been well studied in rod-shaped bacteria, but the mechanisms underlying cell division in spherical bacteria are poorly understood. Rod-shaped bacteria harbor regulatory proteins that place and remodel the division machinery during cytokinesis. In the spherical human pathogen Staphylococcus aureus, w...

journal_title：eLife

authors： Eswara PJ,Brzozowski RS,Viola MG,Graham G,Spanoudis C,Trebino C,Jha J,Aubee JI,Thompson KM,Camberg JL,Ramamurthi KS

更新日期：2018-10-02 00:00:00

abstract：:Many organisms lose the capacity to regenerate damaged tissues as they mature. Damaged Drosophila imaginal discs regenerate efficiently early in the third larval instar (L3) but progressively lose this ability. This correlates with reduced damage-responsive expression of multiple genes, including the WNT genes wingles...

journal_title：eLife

authors： Harris RE,Setiawan L,Saul J,Hariharan IK

更新日期：2016-02-03 00:00:00

abstract：:The fitness effects of synonymous mutations, nucleotide changes that do not alter the encoded amino acid, have often been assumed to be neutral, but a growing body of evidence suggests otherwise. We used site-directed mutagenesis coupled with direct measures of competitive fitness to estimate the distribution of fitne...

journal_title：eLife

authors： Lebeuf-Taylor E,McCloskey N,Bailey SF,Hinz A,Kassen R

更新日期：2019-07-19 00:00:00

abstract：:Oncogenic human papillomaviruses (HPV) are small DNA viruses that infect keratinocytes. After HPV binding to cell surface receptors, a cascade of molecular interactions mediates the infectious cellular internalization of virus particles. Aside from the virus itself, important molecular players involved in virus entry ...

journal_title：eLife

authors： Mikuličić S,Finke J,Boukhallouk F,Wüstenhagen E,Sons D,Homsi Y,Reiss K,Lang T,Florin L

更新日期：2019-05-20 00:00:00

abstract：:MyD88 is the main adaptor molecule for TLR and IL-1R family members. Here, we demonstrated that T-cell intrinsic MyD88 signaling is required for proliferation, protection from apoptosis and expression of activation/memory genes during infection with the intracellular parasite Trypanosoma cruzi, as evidenced by transcr...

journal_title：eLife

authors： Oliveira AC,Gomes-Neto JF,Barbosa CD,Granato A,Reis BS,Santos BM,Fucs R,Canto FB,Nakaya HI,Nóbrega A,Bellio M

更新日期：2017-09-12 00:00:00

abstract：:The COVID-19 pandemic has resulted in school closures and distancing requirements that have disrupted both work and family life for many. Concerns exist that these disruptions caused by the pandemic may not have influenced men and women researchers equally. Many medical journals have published papers on the pandemic, ...

journal_title：eLife

authors： Andersen JP,Nielsen MW,Simone NL,Lewiss RE,Jagsi R

更新日期：2020-06-15 00:00:00

abstract：:Interaction between disease-microbiome associations and ageing has not been explored in detail. Here, using age/region-matched sub-sets, we analysed the gut microbiome differences across five major diseases in a multi-cohort dataset constituting more than 2500 individuals from 20 to 89 years old. We show that disease-...

journal_title：eLife

authors： Ghosh TS,Das M,Jeffery IB,O'Toole PW

更新日期：2020-03-11 00:00:00

abstract：:The prefrontal cortex (PFC)'s functions are thought to include working memory, as its activity can reflect information that must be temporarily maintained to realize the current goal. We designed a flexible spatial working memory task that required rats to navigate - after distractions and a delay - to multiple possib...

journal_title：eLife

authors： Böhm C,Lee AK

更新日期：2020-12-24 00:00:00

abstract：:Fruit fly neuroblasts can either self-renew, rest or take on a specialized form, depending on the levels of a protein called Prospero. ...

journal_title：eLife

authors： Pollarolo G,Gonzalez C

更新日期：2014-10-29 00:00:00