Identification of a molecular basis for the juvenile sleep state.

abstract：:Human pluripotent stem cell (hPSC)-derived neural organoids display unprecedented emergent properties. Yet in contrast to the singular neuroepithelial tube from which the entire central nervous system (CNS) develops in vivo, current organoid protocols yield tissues with multiple neuroepithelial units, a.k.a. neural ro...

journal_title：eLife

authors： Knight GT,Lundin BF,Iyer N,Ashton LM,Sethares WA,Willett RM,Ashton RS

更新日期：2018-10-29 00:00:00

abstract：:Making inferences about the computations performed by neuronal circuits from synapse-level connectivity maps is an emerging opportunity in neuroscience. The mushroom body (MB) is well positioned for developing and testing such an approach due to its conserved neuronal architecture, recently completed dense connectome,...

journal_title：eLife

authors： Li F,Lindsey JW,Marin EC,Otto N,Dreher M,Dempsey G,Stark I,Bates AS,Pleijzier MW,Schlegel P,Nern A,Takemura SY,Eckstein N,Yang T,Francis A,Braun A,Parekh R,Costa M,Scheffer LK,Aso Y,Jefferis GS,Abbott LF,Litwi

更新日期：2020-12-14 00:00:00

abstract：:Animals seek out relevant information by moving through a dynamic world, but sensory systems are usually studied under highly constrained and passive conditions that may not probe important dimensions of the neural code. Here, we explored neural coding in the barrel cortex of head-fixed mice that tracked walls with th...

journal_title：eLife

authors： Sofroniew NJ,Vlasov YA,Hires SA,Freeman J,Svoboda K

更新日期：2015-12-23 00:00:00

abstract：:The knirps (kni) locus encodes transcription factors required for induction of the L2 wing vein in Drosophila. Here, we employ diverse CRISPR/Cas9 genome editing tools to generate a series of targeted lesions within the endogenous cis-regulatory module (CRM) required for kni expression in the L2 vein primordium. Pheno...

journal_title：eLife

authors： Xu XS,Gantz VM,Siomava N,Bier E

更新日期：2017-12-23 00:00:00

abstract：:Genomic sequencing has implicated large numbers of genes and de novo mutations as potential disease risk factors. A high throughput in vivo model system is needed to validate gene associations with pathology. We developed a Drosophila-based functional system to screen candidate disease genes identified from Congenital...

journal_title：eLife

authors： Zhu JY,Fu Y,Nettleton M,Richman A,Han Z

更新日期：2017-01-20 00:00:00

abstract：:The investigation of prosocial behavior is of particular interest from an evolutionary perspective. Comparisons of prosociality across non-human animal species have, however, so far largely focused on primates, and their interpretation is hampered by the diversity of paradigms and procedures used. Here, we present the...

journal_title：eLife

authors： Horn L,Bugnyar T,Griesser M,Hengl M,Izawa EI,Oortwijn T,Rössler C,Scheer C,Schiestl M,Suyama M,Taylor AH,Vanhooland LC,von Bayern AM,Zürcher Y,Massen JJ

更新日期：2020-10-20 00:00:00

abstract：:Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans after silicone oil (S...

journal_title：eLife

authors： Zhang J,Li L,Huang H,Fang F,Webber HC,Zhuang P,Liu L,Dalal R,Tang PH,Mahajan VB,Sun Y,Li S,Zhang M,Goldberg JL,Hu Y

更新日期：2019-05-15 00:00:00

abstract：:Perturbation of neuronal activity is key to understanding the brain's functional properties, however, intervention studies typically perturb neurons in a nonspecific manner. Recent optogenetics techniques have enabled patterned perturbations, in which specific patterns of activity can be invoked in identified target n...

journal_title：eLife

authors： Sadeh S,Clopath C

更新日期：2020-02-19 00:00:00

abstract：:Comprehensively elucidating the molecular mechanisms of human immunodeficiency virus type 1 (HIV-1) latency is a priority to achieve a functional cure. As current 'shock' agents failed to efficiently reactivate the latent reservoir, it is important to discover new targets for developing more efficient latency-reversin...

journal_title：eLife

authors： Ma X,Yang T,Luo Y,Wu L,Jiang Y,Song Z,Pan T,Liu B,Liu G,Liu J,Yu F,He Z,Zhang W,Yang J,Liang L,Guan Y,Zhang X,Li L,Cai W,Tang X,Gao S,Deng K,Zhang H

更新日期：2019-01-17 00:00:00

abstract：:The actomyosin ring generates force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organization and the mechanism of contractility is not well understood. We quantified actin filament order in human cells using fluorescence polarization microscopy and found that cleavage furrow ingression...

journal_title：eLife

authors： Spira F,Cuylen-Haering S,Mehta S,Samwer M,Reversat A,Verma A,Oldenbourg R,Sixt M,Gerlich DW

更新日期：2017-11-06 00:00:00

abstract：:Innate lymphoid cells (ILCs) contribute to host defence and tissue repair but can induce immunopathology. Recent work has revealed tissue-specific roles for ILCs; however, the question of how a small population has large effects on immune homeostasis remains unclear. We identify two mechanisms that ILC3s utilise to ex...

journal_title：eLife

authors： Pearson C,Thornton EE,McKenzie B,Schaupp AL,Huskens N,Griseri T,West N,Tung S,Seddon BP,Uhlig HH,Powrie F

更新日期：2016-01-18 00:00:00

abstract：:GPIHBP1, a GPI-anchored protein of capillary endothelial cells, binds lipoprotein lipase (LPL) within the subendothelial spaces and shuttles it to the capillary lumen. GPIHBP1-bound LPL is essential for the margination of triglyceride-rich lipoproteins (TRLs) along capillaries, allowing the lipolytic processing of TRL...

journal_title：eLife

authors： Hu X,Matsumoto K,Jung RS,Weston TA,Heizer PJ,He C,Sandoval NP,Allan CM,Tu Y,Vinters HV,Liau LM,Ellison RM,Morales JE,Baufeld LJ,Bayley NA,He L,Betsholtz C,Beigneux AP,Nathanson DA,Gerhardt H,Young SG,Fong LG,J

更新日期：2019-06-06 00:00:00

abstract：:During sleep, the brain experiences large fluctuations in blood volume and altered coupling between neural and vascular signals. ...

journal_title：eLife

authors： Williams SD,Lewis LD

更新日期：2020-12-23 00:00:00

abstract：:In all living organisms, ribosomes translating membrane proteins are targeted to membrane translocons early in translation, by the ubiquitous signal recognition particle (SRP) system. In eukaryotes, the SRP Alu domain arrests translation elongation of membrane proteins until targeting is complete. Curiously, however, ...

journal_title：eLife

authors： Fluman N,Navon S,Bibi E,Pilpel Y

更新日期：2014-08-18 00:00:00

abstract：:Dephosphorylation of eukaryotic translation initiation factor 2a (eIF2a) restores protein synthesis at the waning of stress responses and requires a PP1 catalytic subunit and a regulatory subunit, PPP1R15A/GADD34 or PPP1R15B/CReP. Surprisingly, PPP1R15-PP1 binary complexes reconstituted in vitro lacked substrate selec...

journal_title：eLife

authors： Chen R,Rato C,Yan Y,Crespillo-Casado A,Clarke HJ,Harding HP,Marciniak SJ,Read RJ,Ron D

更新日期：2015-03-16 00:00:00

abstract：:The transcription factor ASCIZ (ATMIN, ZNF822) has an unusually high number of recognition motifs for the product of its main target gene, the hub protein LC8 (DYNLL1). Using a combination of biophysical methods, structural analysis by NMR and electron microscopy, and cellular transcription assays, we developed a mode...

journal_title：eLife

authors： Clark S,Myers JB,King A,Fiala R,Novacek J,Pearce G,Heierhorst J,Reichow SL,Barbar EJ

更新日期：2018-05-01 00:00:00

abstract：:Signal transduction at vertebrate excitatory synapses involves the rapid activation of AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole propionate) receptors, glutamate-gated ion channels whose four subunits assemble as a dimer-of-dimers. Technical advances in cryo-electron microscopy brought a slew of full-length structu...

journal_title：eLife

authors： Baranovic J,Plested AJ

更新日期：2018-12-06 00:00:00

abstract：:The sensitivity of ocular dominance to regulation by monocular deprivation is the canonical model of plasticity confined to a critical period. However, we have previously shown that visual deprivation through dark exposure (DE) reactivates critical period plasticity in adults. Previous work assumed that the eliminatio...

journal_title：eLife

authors： Murase S,Lantz CL,Quinlan EM

更新日期：2017-09-06 00:00:00

abstract：:How producers of public goods persist in microbial communities is a major question in evolutionary biology. Cooperation is evolutionarily unstable, since cheating strains can reproduce quicker and take over. Spatial structure has been shown to be a robust mechanism for the evolution of cooperation. Here we study how s...

journal_title：eLife

authors： Uppal G,Vural DC

更新日期：2018-05-22 00:00:00

abstract：:Trypanosome parasites are hiding in human skin, a discovery that may undermine efforts to eliminate sleeping sickness by 2020. ...

journal_title：eLife

authors： Casas-Sánchez A,Acosta-Serrano Á

更新日期：2016-10-14 00:00:00

abstract：:The final identity of a differentiated neuron is determined by multiple signaling events, including activity dependent calcium transients. Non-canonical Frizzled2 (Fz2) signaling generates calcium transients that determine neuronal polarity, neuronal migration, and synapse assembly in the developing vertebrate brain. ...

journal_title：eLife

authors： Agrawal T,Hasan G

更新日期：2015-05-08 00:00:00

abstract：:Optogenetic approaches are leading to a better understanding of the forces that determine the plane of cell division. ...

journal_title：eLife

authors： Serra-Marques A,Dumont S

更新日期：2018-07-09 00:00:00

abstract：:Initiatives to improve research communication can benefit from listening to graduate students, postdocs and newly-independent group leaders. ...

journal_title：eLife

authors： Patterson M,Schekman R

更新日期：2018-03-27 00:00:00

abstract：:Appropriate generalization of learned responses to new situations is vital for adaptive behavior. We provide a circuit-level account of generalization in the electrosensory lobe (ELL) of weakly electric mormyrid fish. Much is already known in this system about a form of learning in which motor corollary discharge sign...

journal_title：eLife

authors： Dempsey C,Abbott LF,Sawtell NB

更新日期：2019-03-14 00:00:00

abstract：:Learning more about the cells that enable parasitic worms called schistosomes to reproduce inside snails could lead to new treatments that prevent these parasites from being transmitted to humans. ...

journal_title：eLife

authors： Sánchez Alvarado A

更新日期：2013-07-30 00:00:00

abstract：:Adult-born neurons are continually produced in the dentate gyrus but it is unclear whether synaptic integration of new neurons affects the pre-existing circuit. Here we investigated how manipulating neurogenesis in adult mice alters excitatory synaptic transmission to mature dentate neurons. Enhancing neurogenesis by ...

journal_title：eLife

authors： Adlaf EW,Vaden RJ,Niver AJ,Manuel AF,Onyilo VC,Araujo MT,Dieni CV,Vo HT,King GD,Wadiche JI,Overstreet-Wadiche L

更新日期：2017-01-30 00:00:00

abstract：:Plasma Membrane is the primary structure for adjusting to ever changing conditions. PM sub-compartmentalization in domains is thought to orchestrate signaling. Yet, mechanisms governing membrane organization are mostly uncharacterized. The plant-specific REMORINs are proteins regulating hormonal crosstalk and host inv...

journal_title：eLife

authors： Gronnier J,Crowet JM,Habenstein B,Nasir MN,Bayle V,Hosy E,Platre MP,Gouguet P,Raffaele S,Martinez D,Grelard A,Loquet A,Simon-Plas F,Gerbeau-Pissot P,Der C,Bayer EM,Jaillais Y,Deleu M,Germain V,Lins L,Mongrand S

更新日期：2017-07-31 00:00:00

abstract：:Visual perception and imagery rely on similar representations in the visual cortex. During perception, visual activity is characterized by distinct processing stages, but the temporal dynamics underlying imagery remain unclear. Here, we investigated the dynamics of visual imagery in human participants using magnetoenc...

journal_title：eLife

authors： Dijkstra N,Mostert P,Lange FP,Bosch S,van Gerven MA

更新日期：2018-05-29 00:00:00

abstract：:Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our approach uses short homology of 24-...

journal_title：eLife

authors： Wierson WA,Welker JM,Almeida MP,Mann CM,Webster DA,Torrie ME,Weiss TJ,Kambakam S,Vollbrecht MK,Lan M,McKeighan KC,Levey J,Ming Z,Wehmeier A,Mikelson CS,Haltom JA,Kwan KM,Chien CB,Balciunas D,Ekker SC,Clark KJ,We

更新日期：2020-05-15 00:00:00

abstract：:Toxoplasma gondii contains a limited subset of actin binding proteins. Here we show that the putative actin regulator cyclase-associated protein (CAP) is present in two different isoforms and its deletion leads to significant defects in some but not all actin dependent processes. We observe defects in cell-cell commun...

journal_title：eLife

authors： Hunt A,Russell MRG,Wagener J,Kent R,Carmeille R,Peddie CJ,Collinson L,Heaslip A,Ward GE,Treeck M

更新日期：2019-10-02 00:00:00