Abstract:
:Stem cells are maintained in a specialized microenvironment called niche but the nature of stem cell niche remains poorly defined in many systems. Here we demonstrate that intestinal epithelium-derived BMP serves as a niche signal for intestinal stem cell (ISC) self-renewal in Drosophila adult midgut. We find that BMP signaling is asymmetric between ISC and its differentiated daughter cell. Two BMP ligands, Dpp and Gbb, are produced by enterocytes and act in conjunction to promote ISC self-renewal by antagonizing Notch signaling. Furthermore, the basement membrane-associated type IV collagens regulate ISC self-renewal by confining higher BMP signaling to ISCs. The employment of gut epithelia as a niche for stem cell self-renewal may provide a mechanism for direct communication between the niche and the environment, allowing niche signal production and stem cell number to be fine-tuned in response to various physiological and pathological stimuli. DOI: http://dx.doi.org/10.7554/eLife.01857.001.
journal_name
Elifejournal_title
eLifeauthors
Tian A,Jiang Jdoi
10.7554/eLife.01857subject
Has Abstractpub_date
2014-03-11 00:00:00pages
e01857issn
2050-084Xjournal_volume
3pub_type
杂志文章相关文献
eLife文献大全abstract::We determined differential gene expression in response to high glucose in lymphoblastoid cell lines derived from matched individuals with type 1 diabetes with and without retinopathy. Those genes exhibiting the largest difference in glucose response were assessed for association with diabetic retinopathy in a genome-w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59980
更新日期:2020-11-09 00:00:00
abstract::Microtubules control different aspects of cell polarization. In cells with a radial microtubule system, a pivotal role in setting up asymmetry is attributed to the relative positioning of the centrosome and the nucleus. Here, we show that centrosome loss had no effect on the ability of endothelial cells to polarize an...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.33864
更新日期:2018-03-16 00:00:00
abstract::Previous research showed that spontaneous neuronal activity presents sloppiness: the collective behavior is strongly determined by a small number of parameter combinations, defined as 'stiff' dimensions, while it is insensitive to many others ('sloppy' dimensions). Here, we analyzed neural population activity from the...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53268
更新日期:2020-03-17 00:00:00
abstract::In eukaryotes, intra-chromosomal recombination generates DNA circles, but little is known about how cells react to them. In yeast, partitioning of such circles to the mother cell at mitosis ensures their loss from the population but promotes replicative ageing. Nevertheless, the mechanisms of partitioning are debated....
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03790
更新日期:2014-11-17 00:00:00
abstract::In recent years, functional genomics approaches combining genetic information with bulk RNA-sequencing data have identified the downstream expression effects of disease-associated genetic risk factors through so-called expression quantitative trait locus (eQTL) analysis. Single-cell RNA-sequencing creates enormous opp...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52155
更新日期:2020-03-09 00:00:00
abstract::Amyloid plaques, consisting of deposited beta-amyloid (Aβ), are a neuropathological hallmark of Alzheimer's Disease (AD). Cerebral vessels play a major role in AD, as Aβ is cleared from the brain by pathways involving the cerebrovasculature, most AD patients have cerebrovascular amyloid (cerebral amyloid angiopathy (C...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.29595
更新日期:2017-10-10 00:00:00
abstract::Asymmetric disassembly of the synaptonemal complex (SC) is crucial for proper meiotic chromosome segregation. However, the signaling mechanisms that directly regulate this process are poorly understood. Here we show that the mammalian Rho GEF homolog, ECT-2, functions through the conserved RAS/ERK MAP kinase signaling...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12039
更新日期:2016-02-27 00:00:00
abstract::In hypoxic stress conditions, glycolysis enzymes assemble into singular cytoplasmic granules called glycolytic (G) bodies. G body formation in yeast correlates with increased glucose consumption and cell survival. However, the physical properties and organizing principles that define G body formation are unclear. We d...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.48480
更新日期:2020-04-16 00:00:00
abstract::We generated induced excitatory neurons (iNeurons, iNs) from chimpanzee, bonobo, and human stem cells by expressing the transcription factor neurogenin-2 (NGN2). Single-cell RNA sequencing showed that genes involved in dendrite and synapse development are expressed earlier during iNs maturation in the chimpanzee and b...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.59323
更新日期:2021-01-20 00:00:00
abstract::Organisms possessing genetic codes with unassigned codons raise the question of how cellular machinery resolves such codons and how this could impact horizontal gene transfer. Here, we use a genomically recoded Escherichia coli to examine how organisms address translation at unassigned UAG codons, which obstruct propa...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.34878
更新日期:2018-10-30 00:00:00
abstract::The knirps (kni) locus encodes transcription factors required for induction of the L2 wing vein in Drosophila. Here, we employ diverse CRISPR/Cas9 genome editing tools to generate a series of targeted lesions within the endogenous cis-regulatory module (CRM) required for kni expression in the L2 vein primordium. Pheno...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30281
更新日期:2017-12-23 00:00:00
abstract::Although individuals of many species inexorably age, a number of observations established that the rate of aging is modulated in response to a variety of mild stresses. Here, we investigated how heat stress promotes longevity in yeast. We show that upon growth at higher temperature, yeast cells relax the retention of ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.28329
更新日期:2017-12-28 00:00:00
abstract::Linking interindividual differences in psychological phenotype to variations in brain structure is an old dream for psychology and a crucial question for cognitive neurosciences. Yet, replicability of the previously-reported 'structural brain behavior' (SBB)-associations has been questioned, recently. Here, we conduct...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.43464
更新日期:2019-03-13 00:00:00
abstract::Knowledge of the host factors required for norovirus replication has been hindered by the challenges associated with culturing human noroviruses. We have combined proteomic analysis of the viral translation and replication complexes with a CRISPR screen, to identify host factors required for norovirus infection. The c...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46681
更新日期:2019-08-12 00:00:00
abstract::Daily rhythms of food anticipatory activity (FAA) are regulated independently of the suprachiasmatic nucleus, which mediates entrainment of rhythms to light, but the neural circuits that establish FAA remain elusive. In this study, we show that mice lacking the dopamine D1 receptor (D1R KO mice) manifest greatly reduc...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03781
更新日期:2014-09-12 00:00:00
abstract::Meiotic drive is the preferential transmission of a particular allele during sexual reproduction. The phenomenon is observed as spore killing in multiple fungi. In natural populations of Podospora anserina, seven spore killer types (Psks) have been identified through classical genetic analyses. Here we show that the S...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46454
更新日期:2019-07-26 00:00:00
abstract::Although several sleep-regulating neuronal populations have been identified, little is known about how they interact with each other to control sleep/wake states. We previously identified neuropeptide VF (NPVF) and the hypothalamic neurons that produce it as a sleep-promoting system (Lee et al., 2017). Here we show us...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54491
更新日期:2020-12-18 00:00:00
abstract::In humans, large conductance voltage- and calcium-dependent potassium (BK) channels are regulated allosterically by transmembrane voltage and intracellular Ca2+. Divalent cation binding sites reside within the gating ring formed by two Regulator of Conductance of Potassium (RCK) domains per subunit. Using patch-clamp ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40664
更新日期:2018-12-11 00:00:00
abstract::The spinal cord has the capacity to coordinate motor activities such as locomotion. Following spinal transection, functional activity can be regained, to a degree, following motor training. To identify microcircuits involved in this recovery, we studied a population of mouse spinal interneurons known to receive direct...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.21715
更新日期:2016-12-15 00:00:00
abstract::The basal ganglia are known to be involved in the planning, execution and control of gripping force and movement vigour. Here we aim to define the nature of the basal ganglia control signal for force and to decode gripping force based on local field potential (LFP) activities recorded from the subthalamic nucleus (STN...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.19089
更新日期:2016-11-18 00:00:00
abstract::The pressure for every research article to tell a clear story often leads researchers in the life sciences to exclude experiments that 'did not work' when they write up their results. However, this practice can lead to reporting bias if the decisions about which experiments to exclude are taken after data have been co...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56626
更新日期:2020-05-22 00:00:00
abstract::Allosteric HIV-1 integrase (IN) inhibitors (ALLINIs) are a promising new class of antiretroviral agents that disrupt proper viral maturation by inducing hyper-multimerization of IN. Here we show that lead pyridine-based ALLINI KF116 exhibits striking selectivity for IN tetramers versus lower order protein oligomers. I...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46344
更新日期:2019-05-23 00:00:00
abstract::The TAM receptor tyrosine kinases Tyro3, Axl, and Mer regulate key features of cellular physiology, yet the differential activities of the TAM ligands Gas6 and Protein S are poorly understood. We have used biochemical and genetic analyses to delineate the rules for TAM receptor-ligand engagement and find that the TAMs...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03385
更新日期:2014-09-29 00:00:00
abstract::Two recently discovered transcription factors stop cells from dividing when plants face extreme heat and DNA damage. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.46781
更新日期:2019-04-09 00:00:00
abstract::In addition to their role in desensitization and internalization of G protein-coupled receptors (GPCRs), β-arrestins are essential scaffolds linking GPCRs to Erk1/2 signaling. However, their role in GPCR-operated Erk1/2 activation differs between GPCRs and the underlying mechanism remains poorly characterized. Here, w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23777
更新日期:2017-02-07 00:00:00
abstract::The human face represents a combined set of highly heritable phenotypes, but knowledge on its genetic architecture remains limited, despite the relevance for various fields. A series of genome-wide association studies on 78 facial shape phenotypes quantified from 3-dimensional facial images of 10,115 Europeans identif...
journal_title:eLife
pub_type: 杂志文章,meta分析
doi:10.7554/eLife.49898
更新日期:2019-11-26 00:00:00
abstract::While traditional microbiological freshwater tests focus on the detection of specific bacterial indicator species, including pathogens, direct tracing of all aquatic DNA through metagenomics poses a profound alternative. Yet, in situ metagenomic water surveys face substantial challenges in cost and logistics. Here, we...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.61504
更新日期:2021-01-19 00:00:00
abstract::Visual perception and imagery rely on similar representations in the visual cortex. During perception, visual activity is characterized by distinct processing stages, but the temporal dynamics underlying imagery remain unclear. Here, we investigated the dynamics of visual imagery in human participants using magnetoenc...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.33904
更新日期:2018-05-29 00:00:00
abstract::Mitochondria support synaptic transmission through production of ATP, sequestration of calcium, synthesis of glutamate, and other vital functions. Surprisingly, less than 50% of hippocampal CA1 presynaptic boutons contain mitochondria, raising the question of whether synapses without mitochondria can sustain changes i...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15275
更新日期:2016-12-19 00:00:00
abstract::Mitochondria play important roles in cellular processes and disease, yet little is known about how the transcriptional regime of the mitochondrial genome varies across individuals and tissues. By analyzing >11,000 RNA-sequencing libraries across 36 tissue/cell types, we find considerable variation in mitochondrial-enc...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.41927
更新日期:2019-02-18 00:00:00