Large, three-generation human families reveal post-zygotic mosaicism and variability in germline mutation accumulation.

abstract：:Yes. ...

journal_title：eLife

authors： Goldstein RE

更新日期：2018-07-23 00:00:00

abstract：:Hair follicle (HF) development is orchestrated by coordinated signals from adjacent epithelial and mesenchymal cells. In humans this process only occurs during embryogenesis and viable strategies to induce new HFs in adult skin are lacking. Here, we reveal that activation of Hedgehog (Hh) signaling in adjacent epithel...

journal_title：eLife

authors： Sun X,Are A,Annusver K,Sivan U,Jacob T,Dalessandri T,Joost S,Füllgrabe A,Gerling M,Kasper M

更新日期：2020-03-17 00:00:00

abstract：:Group B Streptococcus (GBS) is the leading cause of invasive bacterial neonatal infections. Late-onset diseases (LOD) occur between 7 and 89 days of life and are largely due to the CC17 GBS hypervirulent clone. We studied the impact of estradiol (E2) and progesterone (P4), which impregnate the fetus during pregnancy, ...

journal_title：eLife

authors： Hays C,Touak G,Bouaboud A,Fouet A,Guignot J,Poyart C,Tazi A

更新日期：2019-11-11 00:00:00

abstract：:Cell-cell communication in multicellular organisms depends on the dynamic and reversible phosphorylation of protein tyrosine residues. The receptor-linked protein tyrosine phosphatases (RPTPs) receive cues from the extracellular environment and are well placed to influence cell signaling. However, the direct events do...

journal_title：eLife

authors： Fearnley GW,Young KA,Edgar JR,Antrobus R,Hay IM,Liang WC,Martinez-Martin N,Lin W,Deane JE,Sharpe HJ

更新日期：2019-03-29 00:00:00

abstract：:Actin dynamics generate forces to deform the membrane and overcome the cell's high turgor pressure during clathrin-mediated endocytosis (CME) in yeast, but precise molecular details are still unresolved. Our previous models predicted that actin filaments of the endocytic meshwork continually polymerize and disassemble...

journal_title：eLife

authors： Lacy MM,Baddeley D,Berro J

更新日期：2019-12-19 00:00:00

abstract：:Prp3 is an essential U4/U6 di-snRNP-associated protein whose functions and molecular mechanisms in pre-mRNA splicing are presently poorly understood. We show by structural and biochemical analyses that Prp3 contains a bipartite U4/U6 di-snRNA-binding region comprising an expanded ferredoxin-like fold, which recognizes...

journal_title：eLife

authors： Liu S,Mozaffari-Jovin S,Wollenhaupt J,Santos KF,Theuser M,Dunin-Horkawicz S,Fabrizio P,Bujnicki JM,Lührmann R,Wahl MC

更新日期：2015-07-10 00:00:00

abstract：:Mutations in PROP1 are the most common cause of hypopituitarism in humans; therefore, unraveling its mechanism of action is highly relevant from a therapeutic perspective. Our current understanding of the role of PROP1 in the pituitary gland is limited to the repression and activation of the pituitary transcription fa...

journal_title：eLife

authors： Pérez Millán MI,Brinkmeier ML,Mortensen AH,Camper SA

更新日期：2016-06-28 00:00:00

abstract：:We recently reported that the C2AB portion of Synaptotagmin 1 (Syt1) could self-assemble into Ca(2+)-sensitive ring-like oligomers on membranes, which could potentially regulate neurotransmitter release. Here we report that analogous ring-like oligomers assemble from the C2AB domains of other Syt isoforms (Syt2, Syt7,...

journal_title：eLife

authors： Zanetti MN,Bello OD,Wang J,Coleman J,Cai Y,Sindelar CV,Rothman JE,Krishnakumar SS

更新日期：2016-07-19 00:00:00

abstract：:The COVID-19 pandemic is a harsh reminder of the fact that, whether in a single human host or a wave of infection across continents, viral dynamics is often a story about the numbers. In this article we provide a one-stop, curated graphical source for the key numbers (based mostly on the peer-reviewed literature) abou...

journal_title：eLife

authors： Bar-On YM,Flamholz A,Phillips R,Milo R

更新日期：2020-04-02 00:00:00

abstract：:Atopic Dermatitis (AD) is a T cell-mediated chronic skin disease and is associated with altered skin barrier integrity. Infants with mutations in genes involved in tissue barrier fitness are predisposed towards inflammatory diseases, but most do not develop or sustain the diseases, suggesting that there exist regulato...

journal_title：eLife

authors： Spidale NA,Malhotra N,Frascoli M,Sylvia K,Miu B,Freeman C,Stadinski BD,Huseby E,Kang J

更新日期：2020-02-17 00:00:00

abstract：:Microbial fitness screens are a key technique in functional genomics. We present an all-in-one solution, pyphe, for automating and improving data analysis pipelines associated with large-scale fitness screens, including image acquisition and quantification, data normalisation, and statistical analysis. Pyphe is versat...

journal_title：eLife

authors： Kamrad S,Rodríguez-López M,Cotobal C,Correia-Melo C,Ralser M,Bähler J

更新日期：2020-06-16 00:00:00

abstract：:Coronaries are essential for myocardial growth and heart function. Notch is crucial for mouse embryonic angiogenesis, but its role in coronary development remains uncertain. We show Jag1, Dll4 and activated Notch1 receptor expression in sinus venosus (SV) endocardium. Endocardial Jag1 removal blocks SV capillary sprou...

journal_title：eLife

authors： Travisano SI,Oliveira VL,Prados B,Grego-Bessa J,Piñeiro-Sabarís R,Bou V,Gómez MJ,Sánchez-Cabo F,MacGrogan D,de la Pompa JL

更新日期：2019-12-04 00:00:00

abstract：:The brain needs to predict how the body reacts to motor commands, but how a network of spiking neurons can learn non-linear body dynamics using local, online and stable learning rules is unclear. Here, we present a supervised learning scheme for the feedforward and recurrent connections in a network of heterogeneous s...

journal_title：eLife

authors： Gilra A,Gerstner W

更新日期：2017-11-27 00:00:00

abstract：:Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and rea...

journal_title：eLife

authors： Rodrigo Albors A,Tazaki A,Rost F,Nowoshilow S,Chara O,Tanaka EM

更新日期：2015-11-14 00:00:00

abstract：:Analysis of the spatial distribution of endomembrane trafficking is fundamental to understand the mechanisms controlling cellular dynamics, cell homeostasy, and cell interaction with its external environment in normal and pathological situations. We present a semi-parametric framework to quantitatively analyze and vis...

journal_title：eLife

authors： Pécot T,Zengzhen L,Boulanger J,Salamero J,Kervrann C

更新日期：2018-08-09 00:00:00

abstract：:Synaptic positions underlie precise circuit connectivity. Synaptic positions can be established during embryogenesis and sustained during growth. The mechanisms that sustain synaptic specificity during allometric growth are largely unknown. We performed forward genetic screens in C. elegans for regulators of this proc...

journal_title：eLife

authors： Fan J,Ji T,Wang K,Huang J,Wang M,Manning L,Dong X,Shi Y,Zhang X,Shao Z,Colón-Ramos DA

更新日期：2020-04-07 00:00:00

abstract：:Rising and more variable global temperatures pose a challenge for biodiversity, with reproduction and fertility being especially sensitive to heat. Here, we assessed the potential for thermal adaptation in sperm and egg function using Tribolium flour beetles, a warm-temperate-tropical insect model. Following temperatu...

journal_title：eLife

authors： Vasudeva R,Sutter A,Sales K,Dickinson ME,Lumley AJ,Gage MJ

更新日期：2019-10-01 00:00:00

abstract：:The gut microbiota of patients recently diagnosed with rheumatoid arthritis is enriched in microbes belonging to the Prevotella genus. ...

journal_title：eLife

authors： Mathis D

更新日期：2013-11-05 00:00:00

abstract：:Dephosphorylation of eukaryotic translation initiation factor 2a (eIF2a) restores protein synthesis at the waning of stress responses and requires a PP1 catalytic subunit and a regulatory subunit, PPP1R15A/GADD34 or PPP1R15B/CReP. Surprisingly, PPP1R15-PP1 binary complexes reconstituted in vitro lacked substrate selec...

journal_title：eLife

authors： Chen R,Rato C,Yan Y,Crespillo-Casado A,Clarke HJ,Harding HP,Marciniak SJ,Read RJ,Ron D

更新日期：2015-03-16 00:00:00

abstract：:Events in early life contribute to subsequent risk of asthma; however, the causes and trajectories of childhood wheeze are heterogeneous and do not always result in asthma. Similarly, not all atopic individuals develop wheeze, and vice versa. The reasons for these differences are unclear. Using unsupervised model-base...

journal_title：eLife

authors： Tang HH,Teo SM,Belgrave DC,Evans MD,Jackson DJ,Brozynska M,Kusel MM,Johnston SL,Gern JE,Lemanske RF,Simpson A,Custovic A,Sly PD,Holt PG,Holt KE,Inouye M

更新日期：2018-10-15 00:00:00

abstract：:High-altitude environments require that animals meet the metabolic O2 demands for locomotion and thermogenesis in O2-thin air, but the degree to which convergent metabolic changes have arisen across independent high-altitude lineages or the speed at which such changes arise is unclear. We examined seven high-altitude ...

journal_title：eLife

authors： Dawson NJ,Alza L,Nandal G,Scott GR,McCracken KG

更新日期：2020-07-30 00:00:00

abstract：:A new source of progenitor cells can now be used to study hidden aspects of human lung development and pediatric lung disease. ...

journal_title：eLife

authors： Waghray A,Rajagopal J

更新日期：2017-08-14 00:00:00

abstract：:During sleep, the brain experiences large fluctuations in blood volume and altered coupling between neural and vascular signals. ...

journal_title：eLife

authors： Williams SD,Lewis LD

更新日期：2020-12-23 00:00:00

abstract：:Calcineurin is responsible for mediating a wide variety of cellular processes in response to dynamic calcium (Ca(2+)) signals, yet the precise mechanisms involved in the spatiotemporal control of calcineurin signaling are poorly understood. Here, we use genetically encoded fluorescent biosensors to directly probe the ...

journal_title：eLife

authors： Mehta S,Aye-Han NN,Ganesan A,Oldach L,Gorshkov K,Zhang J

更新日期：2014-07-23 00:00:00

abstract：:The orbitofrontal cortex (OFC) is critical for updating reward-directed behaviours flexibly when outcomes are devalued or when task contingencies are reversed. Failure to update behaviour in outcome devaluation and reversal learning procedures are considered canonical deficits following OFC lesions in non-human primat...

journal_title：eLife

authors： Panayi MC,Killcross S

更新日期：2018-07-25 00:00:00

abstract：:In order to assess the contribution of a central clock in the hypothalamic suprachiasmatic nucleus (SCN) to circadian behavior and the organization of peripheral clocks, we generated forebrain/SCN-specific Bmal1 knockout mice by using floxed Bmal1 and pan-neuronal Cre lines. The forebrain knockout mice showed >90% del...

journal_title：eLife

authors： Izumo M,Pejchal M,Schook AC,Lange RP,Walisser JA,Sato TR,Wang X,Bradfield CA,Takahashi JS

更新日期：2014-12-19 00:00:00

abstract：:We reveal a novel mechanism that explains how preparatory activity can evolve in motor-related cortical areas without prematurely inducing movement. The smooth eye movement region of the frontal eye fields (FEFSEM) is a critical node in the neural circuit controlling smooth pursuit eye movement. Preparatory activity e...

journal_title：eLife

authors： Darlington TR,Lisberger SG

更新日期：2020-02-21 00:00:00

abstract：:When a plant cell divides, four related proteins control the trafficking of vesicles and ensure that cargo that is normally recycled to the plasma membrane is instead re-routed to the plane of cell division. ...

journal_title：eLife

authors： Robatzek S

更新日期：2014-04-08 00:00:00

abstract：:Canonical transient receptor potential channels (TRPC) are involved in receptor-operated and/or store-operated Ca2+ signaling. Inhibition of TRPCs by small molecules was shown to be promising in treating renal diseases. In cells, the channels are regulated by calmodulin (CaM). Molecular details of both CaM and drug bi...

journal_title：eLife

authors： Vinayagam D,Quentin D,Yu-Strzelczyk J,Sitsel O,Merino F,Stabrin M,Hofnagel O,Yu M,Ledeboer MW,Nagel G,Malojcic G,Raunser S

更新日期：2020-11-25 00:00:00

abstract：:Goal-directed behavior involves distributed neuronal circuits in the mammalian brain, including diverse regions of neocortex. However, the cellular basis of long-range cortico-cortical signaling during goal-directed behavior is poorly understood. Here, we recorded membrane potential of excitatory layer 2/3 pyramidal n...

journal_title：eLife

authors： Yamashita T,Petersen CCh

更新日期：2016-06-21 00:00:00