Abstract:
:In order to assess the contribution of a central clock in the hypothalamic suprachiasmatic nucleus (SCN) to circadian behavior and the organization of peripheral clocks, we generated forebrain/SCN-specific Bmal1 knockout mice by using floxed Bmal1 and pan-neuronal Cre lines. The forebrain knockout mice showed >90% deletion of BMAL1 in the SCN and exhibited an immediate and complete loss of circadian behavior in constant conditions. Circadian rhythms in peripheral tissues persisted but became desynchronized and damped in constant darkness. The loss of synchrony was rescued by light/dark cycles and partially by restricted feeding (only in the liver and kidney but not in the other tissues) in a distinct manner. These results suggest that the forebrain/SCN is essential for internal temporal order of robust circadian programs in peripheral clocks, and that individual peripheral clocks are affected differently by light and feeding in the absence of a functional oscillator in the forebrain.
journal_name
Elifejournal_title
eLifeauthors
Izumo M,Pejchal M,Schook AC,Lange RP,Walisser JA,Sato TR,Wang X,Bradfield CA,Takahashi JSdoi
10.7554/eLife.04617subject
Has Abstractpub_date
2014-12-19 00:00:00issn
2050-084Xjournal_volume
3pub_type
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