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eLife文献大全abstract::Plasmodium sporozoites, the mosquito-transmitted forms of the malaria parasite, first infect the liver for an initial round of replication before the emergence of pathogenic blood stages. Sporozoites represent attractive targets for antimalarial preventive strategies, yet the mechanisms of parasite entry into hepatocy...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.25903
更新日期:2017-05-16 00:00:00
abstract::To initiate DNA replication, cells first load an MCM helicase double hexamer at origins in a reaction requiring ORC, Cdc6, and Cdt1, also called pre-replicative complex (pre-RC) assembly. The essential mechanistic role of Cdc6 ATP hydrolysis in this reaction is still incompletely understood. Here, we show that althoug...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.05795
更新日期:2015-08-25 00:00:00
abstract::Engineered molecules based on human cytokines have potential uses in research and medicine. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.27198
更新日期:2017-05-12 00:00:00
abstract::SynGAP is a postsynaptic density (PSD) protein that binds to PDZ domains of the scaffold protein PSD-95. We previously reported that heterozygous deletion of Syngap1 in mice is correlated with increased steady-state levels of other key PSD proteins that bind PSD-95, although the level of PSD-95 remains constant (Walku...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52656
更新日期:2020-01-15 00:00:00
abstract::Human pluripotent stem cell (hPSC)-derived neural organoids display unprecedented emergent properties. Yet in contrast to the singular neuroepithelial tube from which the entire central nervous system (CNS) develops in vivo, current organoid protocols yield tissues with multiple neuroepithelial units, a.k.a. neural ro...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.37549
更新日期:2018-10-29 00:00:00
abstract::Most evolutionary thinking is based on the notion of fitness and related ideas such as fitness landscapes and evolutionary optima. Nevertheless, it is often unclear what fitness actually is, and its meaning often depends on the context. Here we argue that fitness should not be a basal ingredient in verbal or mathemati...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.23804
更新日期:2017-02-15 00:00:00
abstract::A well-established cascade of transcription factor (TF) activity orchestrates adipogenesis in response to chemical cues, yet how cell-intrinsic determinants of differentiation such as cell shape and/or seeding density inform this transcriptional program remain enigmatic. Here, we uncover a novel mechanism licensing tr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.06821
更新日期:2015-06-25 00:00:00
abstract::Hair follicle (HF) development is orchestrated by coordinated signals from adjacent epithelial and mesenchymal cells. In humans this process only occurs during embryogenesis and viable strategies to induce new HFs in adult skin are lacking. Here, we reveal that activation of Hedgehog (Hh) signaling in adjacent epithel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46756
更新日期:2020-03-17 00:00:00
abstract::The sensitivity of ocular dominance to regulation by monocular deprivation is the canonical model of plasticity confined to a critical period. However, we have previously shown that visual deprivation through dark exposure (DE) reactivates critical period plasticity in adults. Previous work assumed that the eliminatio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.27345
更新日期:2017-09-06 00:00:00
abstract::In the fruit fly optic lobe, T4 and T5 cells represent the first direction-selective neurons, with T4 cells responding selectively to moving brightness increments (ON) and T5 cells to brightness decrements (OFF). Both T4 and T5 cells comprise four subtypes with directional tuning to one of the four cardinal directions...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.29044
更新日期:2017-08-22 00:00:00
abstract::Replication-dependent (RD) core histone mRNA produced during S-phase is the only known metazoan protein-coding mRNA presenting a 3' stem-loop instead of the otherwise universal polyA tail. A metallo β-lactamase (MBL) fold enzyme, cleavage and polyadenylation specificity factor 73 (CPSF73), is proposed to be the sole e...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.39865
更新日期:2018-12-03 00:00:00
abstract::We present the development and in vivo application of a feedback-based tracking microscope to follow individual mitochondria in sensory neurons of zebrafish larvae with nanometer precision and millisecond temporal resolution. By combining various technical improvements, we tracked individual mitochondria with unpreced...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.46059
更新日期:2019-06-10 00:00:00
abstract::Heterochromatin exerts a heritable form of eukaryotic gene repression and contributes to chromosome segregation fidelity and genome stability. However, to date there has been no quantitative evaluation of the stability of heterochromatic gene repression. We designed a genetic strategy to capture transient losses of ge...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.05007
更新日期:2015-01-12 00:00:00
abstract::Proprioception, the sense of body position, movement, and associated forces, remains poorly understood, despite its critical role in movement. Most studies of area 2, a proprioceptive area of somatosensory cortex, have simply compared neurons' activities to the movement of the hand through space. Using motion tracking...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.48198
更新日期:2020-01-23 00:00:00
abstract::Glucocorticoid receptors (GR) have diverse functions relevant to maintenance of homeostasis and adaptation to environmental challenges. Understanding the importance of tissue-specific GR function in physiology and behavior has been hampered by near-ubiquitous localization in brain and body. Here we use CRISPR/Cas9 gen...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.44672
更新日期:2019-07-22 00:00:00
abstract::Over the past century, soybean oil (SBO) consumption in the United States increased dramatically. The main SBO fatty acid, linoleic acid (18:2), inhibits in vitro the growth of lactobacilli, beneficial members of the small intestinal microbiota. Human-associated lactobacilli have declined in prevalence in Western micr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.32581
更新日期:2018-03-27 00:00:00
abstract::Comprehensively elucidating the molecular mechanisms of human immunodeficiency virus type 1 (HIV-1) latency is a priority to achieve a functional cure. As current 'shock' agents failed to efficiently reactivate the latent reservoir, it is important to discover new targets for developing more efficient latency-reversin...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.42426
更新日期:2019-01-17 00:00:00
abstract::In endoplasmic reticulum-associated protein degradation (ERAD), membrane proteins are ubiquitinated, extracted from the membrane, and degraded by the proteasome. The cytosolic ATPase Cdc48 drives extraction by pulling on polyubiquitinated substrates. How hydrophobic transmembrane (TM) segments are moved from the phosp...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56945
更新日期:2020-06-26 00:00:00
abstract::Perturbation of neuronal activity is key to understanding the brain's functional properties, however, intervention studies typically perturb neurons in a nonspecific manner. Recent optogenetics techniques have enabled patterned perturbations, in which specific patterns of activity can be invoked in identified target n...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52757
更新日期:2020-02-19 00:00:00
abstract::Nervous systems distinguish between self- and other-generated movements by monitoring discrepancies between planned and performed actions. To do so, corollary discharges are conveyed to sensory areas and gate expected reafference. Such gating is observed in neonatal rats during wake-related movements. In contrast, twi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.18749
更新日期:2016-08-03 00:00:00
abstract::The characteristics of goal-directed actions tend to resemble those of previously executed actions, but it is unclear whether such effects depend strictly on action history, or also reflect context-dependent processes related to predictive motor planning. Here we manipulated the time available to initiate movements af...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26713
更新日期:2017-10-23 00:00:00
abstract::Comparing the brains of related species faces the challenges of establishing homologies whilst accommodating evolutionary specializations. Here we propose a general framework for understanding similarities and differences between the brains of primates. The approach uses white matter blueprints of the whole cortex bas...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.35237
更新日期:2018-05-11 00:00:00
abstract::Pervasive expression of circular RNA is a recently discovered feature of eukaryotic gene expression programs, yet its function remains largely unknown. The presumed biogenesis of these RNAs involves a non-canonical 'backsplicing' event. Recent studies in mammalian cell culture posit that backsplicing is facilitated by...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07540
更新日期:2015-06-09 00:00:00
abstract::Innate lymphoid cells (ILCs) contribute to host defence and tissue repair but can induce immunopathology. Recent work has revealed tissue-specific roles for ILCs; however, the question of how a small population has large effects on immune homeostasis remains unclear. We identify two mechanisms that ILC3s utilise to ex...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10066
更新日期:2016-01-18 00:00:00
abstract::During translation elongation, the ribosome ratchets along its mRNA template, incorporating each new amino acid and translocating from one codon to the next. The elongation cycle requires dramatic structural rearrangements of the ribosome. We show here that deep sequencing of ribosome-protected mRNA fragments reveals ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.01257
更新日期:2014-05-09 00:00:00
abstract::Signal transduction at vertebrate excitatory synapses involves the rapid activation of AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole propionate) receptors, glutamate-gated ion channels whose four subunits assemble as a dimer-of-dimers. Technical advances in cryo-electron microscopy brought a slew of full-length structu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.40548
更新日期:2018-12-06 00:00:00
abstract::Context-dependent memories may guide adaptive behavior relaying in previous experience while updating stored information through reconsolidation. Retrieval can be triggered by partial and shared cues. When the cue is presented, the most relevant memory should be updated. In a contextual version of the object recogniti...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.33746
更新日期:2018-05-02 00:00:00
abstract::Multi-modal MRI techniques have identified biomarkers that could help to predict whether someone will develop epilepsy. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.29853
更新日期:2017-07-26 00:00:00
abstract::The TAM receptor tyrosine kinases Tyro3, Axl, and Mer regulate key features of cellular physiology, yet the differential activities of the TAM ligands Gas6 and Protein S are poorly understood. We have used biochemical and genetic analyses to delineate the rules for TAM receptor-ligand engagement and find that the TAMs...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03385
更新日期:2014-09-29 00:00:00
abstract::The incubation period for typhoid, polio, measles, leukemia and many other diseases follows a right-skewed, approximately lognormal distribution. Although this pattern was discovered more than sixty years ago, it remains an open question to explain its ubiquity. Here, we propose an explanation based on evolutionary dy...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30212
更新日期:2017-12-21 00:00:00