Abstract:
:Over the past century, soybean oil (SBO) consumption in the United States increased dramatically. The main SBO fatty acid, linoleic acid (18:2), inhibits in vitro the growth of lactobacilli, beneficial members of the small intestinal microbiota. Human-associated lactobacilli have declined in prevalence in Western microbiomes, but how dietary changes may have impacted their ecology is unclear. Here, we compared the in vitro and in vivo effects of 18:2 on Lactobacillus reuteri and L. johnsonii. Directed evolution in vitro in both species led to strong 18:2 resistance with mutations in genes for lipid biosynthesis, acid stress, and the cell membrane or wall. Small-intestinal Lactobacillus populations in mice were unaffected by chronic and acute 18:2 exposure, yet harbored both 18:2- sensitive and resistant strains. This work shows that extant small intestinal lactobacilli are protected from toxic dietary components via the gut environment as well as their own capacity to evolve resistance.
journal_name
Elifejournal_title
eLifeauthors
Di Rienzi SC,Jacobson J,Kennedy EA,Bell ME,Shi Q,Waters JL,Lawrence P,Brenna JT,Britton RA,Walter J,Ley REdoi
10.7554/eLife.32581subject
Has Abstractpub_date
2018-03-27 00:00:00issn
2050-084Xpii
32581journal_volume
7pub_type
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