Abstract:
:Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.
journal_name
Elifejournal_title
eLifeauthors
Lleras Forero L,Narayanan R,Huitema LF,VanBergen M,Apschner A,Peterson-Maduro J,Logister I,Valentin G,Morelli LG,Oates AC,Schulte-Merker Sdoi
10.7554/eLife.33843subject
Has Abstractpub_date
2018-04-06 00:00:00issn
2050-084Xpii
33843journal_volume
7pub_type
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