Analysis of SUMO1-conjugation at synapses.

abstract：:New ages for flowstone, sediments and fossil bones from the Dinaledi Chamber are presented. We combined optically stimulated luminescence dating of sediments with U-Th and palaeomagnetic analyses of flowstones to establish that all sediments containing Homo naledi fossils can be allocated to a single stratigraphic ent...

journal_title：eLife

authors： Dirks PH,Roberts EM,Hilbert-Wolf H,Kramers JD,Hawks J,Dosseto A,Duval M,Elliott M,Evans M,Grün R,Hellstrom J,Herries AI,Joannes-Boyau R,Makhubela TV,Placzek CJ,Robbins J,Spandler C,Wiersma J,Woodhead J,Berger LR

更新日期：2017-05-09 00:00:00

abstract：:The integrity of the nuclear membranes coupled to the selective barrier of nuclear pore complexes (NPCs) are essential for the segregation of nucleoplasm and cytoplasm. Mechanical membrane disruption or perturbation to NPC assembly triggers an ESCRT-dependent surveillance system that seals nuclear pores: how these por...

journal_title：eLife

authors： Thaller DJ,Allegretti M,Borah S,Ronchi P,Beck M,Lusk CP

更新日期：2019-04-03 00:00:00

abstract：:Huntington disease (HD) is a neurological disorder caused by polyglutamine expansions in mutated Huntingtin (mHtt) proteins, rendering them prone to form inclusion bodies (IB). We report that in yeast, such IB formation is a factor-dependent process subjected to age-related decline. A genome-wide, high-content imaging...

journal_title：eLife

authors： Yang J,Hao X,Cao X,Liu B,Nyström T

更新日期：2016-04-01 00:00:00

abstract：:Macrophage activation/polarization to distinct functional states is critically supported by metabolic shifts. How polarizing signals coordinate metabolic and functional reprogramming, and the potential implications for control of macrophage activation, remains poorly understood. Here we show that IL-4 signaling co-opt...

journal_title：eLife

authors： Covarrubias AJ,Aksoylar HI,Yu J,Snyder NW,Worth AJ,Iyer SS,Wang J,Ben-Sahra I,Byles V,Polynne-Stapornkul T,Espinosa EC,Lamming D,Manning BD,Zhang Y,Blair IA,Horng T

更新日期：2016-02-19 00:00:00

abstract：:We determined whether the mutations found in ovarian cancers could be identified in the patients' ovarian cyst fluids. Tumor-specific mutations were detectable in the cyst fluids of 19 of 23 (83%) borderline tumors, 10 of 13 (77%) type I cancers, and 18 of 18 (100%) type II cancers. In contrast, no mutations were foun...

journal_title：eLife

authors： Wang Y,Sundfeldt K,Mateoiu C,Shih IeM,Kurman RJ,Schaefer J,Silliman N,Kinde I,Springer S,Foote M,Kristjansdottir B,James N,Kinzler KW,Papadopoulos N,Diaz LA,Vogelstein B

更新日期：2016-07-15 00:00:00

abstract：:Two mammalian genes, Kcnt1 and Kcnt2, encode pore-forming subunits of Na(+)-dependent K(+) (KNa) channels. Progress in understanding KNa channels has been hampered by the absence of specific tools and methods for rigorous KNa identification in native cells. Here, we report the genetic disruption of both Kcnt1 and Kcnt...

journal_title：eLife

authors： Martinez-Espinosa PL,Wu J,Yang C,Gonzalez-Perez V,Zhou H,Liang H,Xia XM,Lingle CJ

更新日期：2015-11-11 00:00:00

abstract：:During development, tissue repair, and tumor growth, most blood vessel networks are generated through angiogenesis. Vascular endothelial growth factor (VEGF) is a key regulator of this process and currently both VEGF and its receptors, VEGFR1, VEGFR2, and Neuropilin1 (NRP1), are targeted in therapeutic strategies for ...

journal_title：eLife

authors： Gelfand MV,Hagan N,Tata A,Oh WJ,Lacoste B,Kang KT,Kopycinska J,Bischoff J,Wang JH,Gu C

更新日期：2014-09-22 00:00:00

abstract：:Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased in...

journal_title：eLife

authors： Wales P,Schuberth CE,Aufschnaiter R,Fels J,García-Aguilar I,Janning A,Dlugos CP,Schäfer-Herte M,Klingner C,Wälte M,Kuhlmann J,Menis E,Hockaday Kang L,Maier KC,Hou W,Russo A,Higgs HN,Pavenstädt H,Vogl T,Roth J,Qual

更新日期：2016-12-06 00:00:00

abstract：:Most funding agencies rely on peer review to evaluate grant applications and proposals, but research into the use of this process by funding agencies has been limited. Here we explore if two changes to the organization of peer review for proposals submitted to various funding actions by the European Union has an influ...

journal_title：eLife

authors： Pina DG,Buljan I,Hren D,Marušić A

更新日期：2021-01-13 00:00:00

abstract：:Aberrant expression of cancer genes and non-canonical RNA species is a hallmark of cancer. However, the mechanisms driving such atypical gene expression programs are incompletely understood. Here, our transcriptional profiling of a cohort of 50 primary clear cell renal cell carcinoma (ccRCC) samples from The Cancer Ge...

journal_title：eLife

authors： Grosso AR,Leite AP,Carvalho S,Matos MR,Martins FB,Vítor AC,Desterro JM,Carmo-Fonseca M,de Almeida SF

更新日期：2015-11-17 00:00:00

abstract：:Journals are exploring new approaches to peer review in order to reduce bias, increase transparency and respond to author preferences. Funders are also getting involved. ...

journal_title：eLife

authors： Rodgers P

更新日期：2017-09-29 00:00:00

abstract：:Autophagy has been linked to longevity in many species, but the underlying mechanisms are unclear. Using a GFP-tagged and a new tandem-tagged Atg8/LGG-1 reporter, we quantified autophagic vesicles and performed autophagic flux assays in multiple tissues of wild-type Caenorhabditis elegans and long-lived daf-2/insulin/...

journal_title：eLife

authors： Chang JT,Kumsta C,Hellman AB,Adams LM,Hansen M

更新日期：2017-07-04 00:00:00

abstract：:Cell-cell communication in multicellular organisms depends on the dynamic and reversible phosphorylation of protein tyrosine residues. The receptor-linked protein tyrosine phosphatases (RPTPs) receive cues from the extracellular environment and are well placed to influence cell signaling. However, the direct events do...

journal_title：eLife

authors： Fearnley GW,Young KA,Edgar JR,Antrobus R,Hay IM,Liang WC,Martinez-Martin N,Lin W,Deane JE,Sharpe HJ

更新日期：2019-03-29 00:00:00

abstract：:Mycobacterium tuberculosis (Mtb) expresses a broad-spectrum β-lactamase (BlaC) that mediates resistance to one of the highly effective antibacterials, β-lactams. Nonetheless, β-lactams showed mycobactericidal activity in combination with β-lactamase inhibitor, clavulanate (Clav). However, the mechanistic aspects of ho...

journal_title：eLife

authors： Mishra S,Shukla P,Bhaskar A,Anand K,Baloni P,Jha RK,Mohan A,Rajmani RS,Nagaraja V,Chandra N,Singh A

更新日期：2017-05-26 00:00:00

abstract：:Experiments on Drosophila have shown that the histones that are normally bound to lipid droplets inside cells can be released to provide protection against infection. ...

journal_title：eLife

authors： Kolter R

更新日期：2012-11-13 00:00:00

abstract：:Recently, a small-molecule communication mechanism was discovered in a range of Bacillus-infecting bacteriophages, which these temperate phages use to inform their lysis-lysogeny decision. We present a mathematical model of the ecological and evolutionary dynamics of such viral communication, and show that a communica...

journal_title：eLife

authors： Doekes HM,Mulder GA,Hermsen R

更新日期：2021-01-18 00:00:00

abstract：:Coat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, linking them to the outer COPII components that form a cage around the vesicle. Regulated flexibility in coat architect...

journal_title：eLife

authors： Zanetti G,Prinz S,Daum S,Meister A,Schekman R,Bacia K,Briggs JA

更新日期：2013-09-17 00:00:00

abstract：:During land plant evolution, determinate spore-bearing axes (retained in extant bryophytes such as mosses) were progressively transformed into indeterminate branching shoots with specialized reproductive axes that form flowers. The LEAFY transcription factor, which is required for the first zygotic cell division in mo...

journal_title：eLife

authors： Plackett AR,Conway SJ,Hewett Hazelton KD,Rabbinowitsch EH,Langdale JA,Di Stilio VS

更新日期：2018-10-24 00:00:00

abstract：:We present the development and in vivo application of a feedback-based tracking microscope to follow individual mitochondria in sensory neurons of zebrafish larvae with nanometer precision and millisecond temporal resolution. By combining various technical improvements, we tracked individual mitochondria with unpreced...

journal_title：eLife

authors： Wehnekamp F,Plucińska G,Thong R,Misgeld T,Lamb DC

更新日期：2019-06-10 00:00:00

abstract：:The Frank-Starling relation is a fundamental auto-regulatory property of the heart that ensures the volume of blood ejected in each heartbeat is matched to the extent of venous filling. At the cellular level, heart muscle cells generate higher force when stretched, but despite intense efforts the underlying molecular ...

journal_title：eLife

authors： Zhang X,Kampourakis T,Yan Z,Sevrieva I,Irving M,Sun YB

更新日期：2017-02-23 00:00:00

abstract：:More than 1500 fossils from the Rising Star cave system in South Africa have been assigned to a new human species, Homo naledi, which displays a unique combination of primitive and derived traits throughout the skeleton. ...

journal_title：eLife

authors： Stringer C

更新日期：2015-09-10 00:00:00

abstract：:Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease involving cytotoxic conformations of Cu, Zn superoxide dismutase (SOD1). A major challenge in understanding ALS disease pathology has been the identification and atomic-level characterization of these conformers. Here, we use a combination ...

journal_title：eLife

authors： Sekhar A,Rumfeldt JA,Broom HR,Doyle CM,Bouvignies G,Meiering EM,Kay LE

更新日期：2015-06-23 00:00:00

abstract：:It is now clear that microglia and macrophages are present in brain tumors, but whether or how they affect initiation and development of tumors is not known. Exploiting the advantages of the zebrafish (Danio rerio) model, we showed that macrophages and microglia respond immediately upon oncogene activation in the brai...

journal_title：eLife

authors： Chia K,Mazzolini J,Mione M,Sieger D

更新日期：2018-02-21 00:00:00

abstract：:The faithful segregation of eukaryotic chromosomes in mitosis requires that the genome be duplicated completely prior to anaphase. However, cells with large genomes sometimes fail to complete replication during interphase and instead enter mitosis with regions of incompletely replicated DNA. These regions are processe...

journal_title：eLife

authors： Sonneville R,Bhowmick R,Hoffmann S,Mailand N,Hickson ID,Labib K

更新日期：2019-09-23 00:00:00

abstract：:Accurate chromosome segregation relies on bioriented amphitelic attachments of chromosomes to microtubules of the mitotic spindle, in which sister chromatids are connected to opposite spindle poles. BUB-1 is a protein of the Spindle Assembly Checkpoint (SAC) that coordinates chromosome attachment with anaphase onset. ...

journal_title：eLife

authors： Edwards F,Maton G,Gareil N,Canman JC,Dumont J

更新日期：2018-12-14 00:00:00

abstract：:The axolotl can regenerate multiple organs, including the brain. It remains, however, unclear whether neuronal diversity, intricate tissue architecture, and axonal connectivity can be regenerated; yet, this is critical for recovery of function and a central aim of cell replacement strategies in the mammalian central n...

journal_title：eLife

authors： Amamoto R,Huerta VG,Takahashi E,Dai G,Grant AK,Fu Z,Arlotta P

更新日期：2016-05-09 00:00:00

abstract：:Hsp90 is a homodimeric ATP-dependent molecular chaperone that remodels its substrate 'client' proteins, facilitating their folding and activating them for biological function. Despite decades of research, the mechanism connecting ATP hydrolysis and chaperone function remains elusive. Particularly puzzling has been the...

journal_title：eLife

authors： Elnatan D,Betegon M,Liu Y,Ramelot T,Kennedy MA,Agard DA

更新日期：2017-07-25 00:00:00

abstract：:Uromodulin is the most abundant protein in the urine. It is exclusively produced by renal epithelial cells and it plays key roles in kidney function and disease. Uromodulin mainly exerts its function as an extracellular matrix whose assembly depends on a conserved, specific proteolytic cleavage leading to conformation...

journal_title：eLife

authors： Brunati M,Perucca S,Han L,Cattaneo A,Consolato F,Andolfo A,Schaeffer C,Olinger E,Peng J,Santambrogio S,Perrier R,Li S,Bokhove M,Bachi A,Hummler E,Devuyst O,Wu Q,Jovine L,Rampoldi L

更新日期：2015-12-17 00:00:00

abstract：: ...

journal_title：eLife

authors： Zhou FY,Ruiz-Puig C,Owen RP,White MJ,Rittscher J,Lu X

更新日期：2019-07-02 00:00:00

abstract：:The phylogenetic placement of the morphologically simple placozoans is crucial to understanding the evolution of complex animal traits. Here, we examine the influence of adding new genomes from placozoans to a large dataset designed to study the deepest splits in the animal phylogeny. Using site-heterogeneous substitu...

journal_title：eLife

authors： Laumer CE,Gruber-Vodicka H,Hadfield MG,Pearse VB,Riesgo A,Marioni JC,Giribet G

更新日期：2018-10-30 00:00:00