Abstract:
:Accurate chromosome segregation relies on bioriented amphitelic attachments of chromosomes to microtubules of the mitotic spindle, in which sister chromatids are connected to opposite spindle poles. BUB-1 is a protein of the Spindle Assembly Checkpoint (SAC) that coordinates chromosome attachment with anaphase onset. BUB-1 is also required for accurate sister chromatid segregation independently of its SAC function, but the underlying mechanism remains unclear. Here we show that, in Caenorhabditis elegans embryos, BUB-1 accelerates the establishment of non-merotelic end-on kinetochore-microtubule attachments by recruiting the RZZ complex and its downstream partner dynein-dynactin at the kinetochore. In parallel, BUB-1 limits attachment maturation by the SKA complex. This activity opposes kinetochore-microtubule attachment stabilisation promoted by CLS-2CLASP-dependent kinetochore-microtubule assembly. BUB-1 is therefore a SAC component that coordinates the function of multiple downstream kinetochore-associated proteins to ensure accurate chromosome segregation.
journal_name
Elifejournal_title
eLifeauthors
Edwards F,Maton G,Gareil N,Canman JC,Dumont Jdoi
10.7554/eLife.40690subject
Has Abstractpub_date
2018-12-14 00:00:00issn
2050-084Xpii
40690journal_volume
7pub_type
杂志文章相关文献
eLife文献大全abstract::WNK1, a kinase that controls kidney salt homeostasis, also regulates adhesion and migration in CD4+ T cells. Wnk1 is highly expressed in thymocytes, and since migration is important for thymocyte maturation, we investigated a role for WNK1 in mouse thymocyte development. We find that WNK1 is required for the transitio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56934
更新日期:2020-10-14 00:00:00
abstract::TFIID-a complex of TATA-binding protein (TBP) and TBP-associated factors (TAFs)-is a central component of the Pol II promoter recognition apparatus. Recent studies have revealed significant downregulation of TFIID subunits in terminally differentiated myocytes, hepatocytes and adipocytes. Here, we report that TBP prot...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.08536
更新日期:2015-09-22 00:00:00
abstract::Voltage-gated sodium channels are targets for a range of pharmaceutical drugs developed for the treatment of neurological diseases. Cannabidiol (CBD), the non-psychoactive compound isolated from cannabis plants, was recently approved for treatment of two types of epilepsy associated with sodium channel mutations. This...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.58593
更新日期:2020-10-22 00:00:00
abstract::Drosophila melanogaster males perform a series of courtship behaviors that, when successful, result in copulation with a female. For over a century, mutations in the yellow gene, named for its effects on pigmentation, have been known to reduce male mating success. Prior work has suggested that yellow influences mating...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49388
更新日期:2019-10-15 00:00:00
abstract::We recently reported that the C2AB portion of Synaptotagmin 1 (Syt1) could self-assemble into Ca(2+)-sensitive ring-like oligomers on membranes, which could potentially regulate neurotransmitter release. Here we report that analogous ring-like oligomers assemble from the C2AB domains of other Syt isoforms (Syt2, Syt7,...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17262
更新日期:2016-07-19 00:00:00
abstract::Habit formation is a behavioral adaptation that automates routine actions. Habitual behavior correlates with broad reconfigurations of dorsolateral striatal (DLS) circuit properties that increase gain and shift pathway timing. The mechanism(s) for these circuit adaptations are unknown and could be responsible for habi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.26231
更新日期:2017-09-05 00:00:00
abstract::Serotonin is implicated in mood and affective disorders. However, growing evidence suggests that a core endogenous role is to promote flexible adaptation to changes in the causal structure of the environment, through behavioral inhibition and enhanced plasticity. We used long-term photometric recordings in mice to stu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.20552
更新日期:2017-03-21 00:00:00
abstract::Islet amyloidosis by IAPP contributes to pancreatic β-cell death in diabetes, but the nature of toxic IAPP species remains elusive. Using concurrent time-resolved biophysical and biological measurements, we define the toxic species produced during IAPP amyloid formation and link their properties to induction of rat IN...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12977
更新日期:2016-05-23 00:00:00
abstract::Dorsal/ventral (DV) patterning of the sea urchin embryo relies on a ventrally-localized organizer expressing Nodal, a pivotal regulator of the DV gene regulatory network. However, the inceptive mechanisms imposing the symmetry-breaking are incompletely understood. In Paracentrotus lividus, the Hbox12 homeodomain-conta...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.04664
更新日期:2014-12-02 00:00:00
abstract::Although several sleep-regulating neuronal populations have been identified, little is known about how they interact with each other to control sleep/wake states. We previously identified neuropeptide VF (NPVF) and the hypothalamic neurons that produce it as a sleep-promoting system (Lee et al., 2017). Here we show us...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54491
更新日期:2020-12-18 00:00:00
abstract::The controversy surrounding 'gain-of-function' experiments on high-consequence avian influenza viruses has highlighted the role of ferret transmission experiments in studying the transmission potential of novel influenza strains. However, the mapping between influenza transmission in ferrets and in humans is unsubstan...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07969
更新日期:2015-09-02 00:00:00
abstract::Over the past century, soybean oil (SBO) consumption in the United States increased dramatically. The main SBO fatty acid, linoleic acid (18:2), inhibits in vitro the growth of lactobacilli, beneficial members of the small intestinal microbiota. Human-associated lactobacilli have declined in prevalence in Western micr...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.32581
更新日期:2018-03-27 00:00:00
abstract::The mesiotemporal lobe (MTL) is implicated in many cognitive processes, is compromised in numerous brain disorders, and exhibits a gradual cytoarchitectural transition from six-layered parahippocampal isocortex to three-layered hippocampal allocortex. Leveraging an ultra-high-resolution histological reconstruction of ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60673
更新日期:2020-11-04 00:00:00
abstract::Actin filaments assemble inside the nucleus in response to multiple cellular perturbations, including heat shock, protein misfolding, integrin engagement, and serum stimulation. We find that DNA damage also generates nuclear actin filaments-detectable by phalloidin and live-cell actin probes-with three characteristic ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.07735
更新日期:2015-08-19 00:00:00
abstract::Epicardial cells on the heart's surface give rise to coronary artery smooth muscle cells (caSMCs) located deep in the myocardium. However, the differentiation steps between epicardial cells and caSMCs are unknown as are the final maturation signals at coronary arteries. Here, we use clonal analysis and lineage tracing...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10036
更新日期:2015-10-19 00:00:00
abstract::Dendrites shape information flow in neurons. Yet, there is little consensus on the level of spatial complexity at which they operate. Through carefully chosen parameter fits, solvable in the least-squares sense, we obtain accurate reduced compartmental models at any level of complexity. We show that (back-propagating)...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60936
更新日期:2021-01-26 00:00:00
abstract::Some DNA viruses overcome plant defenses by producing a suppressor protein that blocks the silencing of viral genes. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11509
更新日期:2015-10-16 00:00:00
abstract::The design of synthetic optogenetic tools that allow precise spatiotemporal control of biological processes previously inaccessible to optogenetic control has developed rapidly over the last years. Rational design of such tools requires detailed knowledge of allosteric light signaling in natural photoreceptors. To und...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11860
更新日期:2016-01-12 00:00:00
abstract::Psychological theories of suicide suggest that certain traits may reduce aversion to physical threat and increase the probability of transitioning from suicidal ideation to action. Here, we investigated whether blunted sensitivity to bodily signals is associated with suicidal action by comparing individuals with a his...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51593
更新日期:2020-04-07 00:00:00
abstract::Systemic oxygen restriction (SOR) is prevalent in numerous clinical conditions, including chronic obstructive pulmonary disease (COPD), and is associated with increased susceptibility to viral infections. However, the influence of SOR on T cell immunity remains uncharacterized. Here we show the detrimental effect of h...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56612
更新日期:2020-11-23 00:00:00
abstract::Binary fission has been well studied in rod-shaped bacteria, but the mechanisms underlying cell division in spherical bacteria are poorly understood. Rod-shaped bacteria harbor regulatory proteins that place and remodel the division machinery during cytokinesis. In the spherical human pathogen Staphylococcus aureus, w...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.38856
更新日期:2018-10-02 00:00:00
abstract::Temperature-sensitive transient receptor potential (TRP) ion channels are members of the large tetrameric cation channels superfamily but are considered to be uniquely sensitive to heat, which has been presumed to be due to the existence of an unidentified temperature-sensing domain. Here we report that the homologous...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.03255
更新日期:2014-07-16 00:00:00
abstract::Our understanding of the antigen presentation pathway has recently been enhanced with the identification that the tapasin-related protein TAPBPR is a second major histocompatibility complex (MHC) class I-specific chaperone. We sought to determine whether, like tapasin, TAPBPR can also influence MHC class I peptide sel...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.09617
更新日期:2015-10-06 00:00:00
abstract::Zebra finches communicate with each other in ways that are more complex than previously thought. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11665
更新日期:2015-10-21 00:00:00
abstract::Engineered molecules based on human cytokines have potential uses in research and medicine. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.27198
更新日期:2017-05-12 00:00:00
abstract::Some cortical neurons receive highly selective thalamocortical (TC) input, but others do not. Here, we examine connectivity of single thalamic neurons (lateral geniculate nucleus, LGN) onto putative fast-spike inhibitory interneurons in layer 4 of rabbit visual cortex. We show that three 'rules' regulate this connecti...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.60102
更新日期:2020-12-08 00:00:00
abstract::A major challenge in neuroscience is to longitudinally monitor whole brain activity across multiple spatial scales in the same animal. Functional UltraSound (fUS) is an emerging technology that offers images of cerebral blood volume over large brain portions. Here we show for the first time its capability to resolve t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.35028
更新日期:2018-06-28 00:00:00
abstract::Cell growth and/or proliferation may require the reprogramming of metabolic pathways, whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates towards anabolic pathways. Herein, we identify a novel role for TRIM32 in the maintenance of glycolytic flux mediated by biochemical interactio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52358
更新日期:2020-03-30 00:00:00
abstract::Animals and humans replay neural patterns encoding trajectories through their environment, both whilst they solve decision-making tasks and during rest. Both on-task and off-task replay are believed to contribute to flexible decision making, though how their relative contributions differ remains unclear. We investigat...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.56911
更新日期:2020-06-17 00:00:00
abstract::The actomyosin ring generates force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organization and the mechanism of contractility is not well understood. We quantified actin filament order in human cells using fluorescence polarization microscopy and found that cleavage furrow ingression...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.30867
更新日期:2017-11-06 00:00:00