Blue light-induced LOV domain dimerization enhances the affinity of Aureochrome 1a for its target DNA sequence.

Abstract:

:The design of synthetic optogenetic tools that allow precise spatiotemporal control of biological processes previously inaccessible to optogenetic control has developed rapidly over the last years. Rational design of such tools requires detailed knowledge of allosteric light signaling in natural photoreceptors. To understand allosteric communication between sensor and effector domains, characterization of all relevant signaling states is required. Here, we describe the mechanism of light-dependent DNA binding of the light-oxygen-voltage (LOV) transcription factor Aureochrome 1a from Phaeodactylum tricornutum (PtAu1a) and present crystal structures of a dark state LOV monomer and a fully light-adapted LOV dimer. In combination with hydrogen/deuterium-exchange, solution scattering data and DNA-binding experiments, our studies reveal a light-sensitive interaction between the LOV and basic region leucine zipper DNA-binding domain that together with LOV dimerization results in modulation of the DNA affinity of PtAu1a. We discuss the implications of these results for the design of synthetic LOV-based photosensors with application in optogenetics.

journal_name

Elife

journal_title

eLife

authors

Heintz U,Schlichting I

doi

10.7554/eLife.11860

subject

Has Abstract

pub_date

2016-01-12 00:00:00

pages

e11860

issn

2050-084X

journal_volume

5

pub_type

杂志文章

相关文献

eLife文献大全
  • Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning.

    abstract::Vertebrate embryonic patterning depends on signaling from Nodal, a TGFβ superfamily member. There are three Nodal orthologs in zebrafish; southpaw directs left-right asymmetries, while squint and cyclops function earlier to pattern mesendoderm. TGFβ member Vg1 is implicated in mesoderm formation but the role of the ze...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.28635

    authors: Pelliccia JL,Jindal GA,Burdine RD

    更新日期:2017-11-15 00:00:00

  • Transcriptional rewiring over evolutionary timescales changes quantitative and qualitative properties of gene expression.

    abstract::Evolutionary changes in transcription networks are an important source of diversity across species, yet the quantitative consequences of network evolution have rarely been studied. Here we consider the transcriptional 'rewiring' of the three GAL genes that encode the enzymes needed for cells to convert galactose to gl...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.18981

    authors: Dalal CK,Zuleta IA,Mitchell KF,Andes DR,El-Samad H,Johnson AD

    更新日期:2016-09-10 00:00:00

  • The spinal cord is never at rest.

    abstract::Even when we are at rest, our spinal cords show spontaneous, yet well organised, fluctuations of activity that might reflect sensory and motor networks. ...

    journal_title:eLife

    pub_type: 评论,杂志文章

    doi:10.7554/eLife.03811

    authors: Eippert F,Tracey I

    更新日期:2014-08-05 00:00:00

  • Experimental evaluation of the importance of colonization history in early-life gut microbiota assembly.

    abstract::The factors that govern assembly of the gut microbiota are insufficiently understood. Here, we test the hypothesis that inter-individual microbiota variation can arise solely from differences in the order and timing by which the gut is colonized early in life. Experiments in which mice were inoculated in sequence eith...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.36521

    authors: Martínez I,Maldonado-Gomez MX,Gomes-Neto JC,Kittana H,Ding H,Schmaltz R,Joglekar P,Cardona RJ,Marsteller NL,Kembel SW,Benson AK,Peterson DA,Ramer-Tait AE,Walter J

    更新日期:2018-09-18 00:00:00

  • Microstructural organization of human insula is linked to its macrofunctional circuitry and predicts cognitive control.

    abstract::The human insular cortex is a heterogeneous brain structure which plays an integrative role in guiding behavior. The cytoarchitectonic organization of the human insula has been investigated over the last century using postmortem brains but there has been little progress in noninvasive in vivo mapping of its microstruc...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.53470

    authors: Menon V,Gallardo G,Pinsk MA,Nguyen VD,Li JR,Cai W,Wassermann D

    更新日期:2020-06-04 00:00:00

  • TORC2-Gad8-dependent myosin phosphorylation modulates regulation by calcium.

    abstract::Cells respond to changes in their environment through signaling networks that modulate cytoskeleton and membrane organization to coordinate cell-cycle progression, polarized cell growth and multicellular development. Here, we define a novel regulatory mechanism by which the motor activity and function of the fission y...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.51150

    authors: Baker K,Gyamfi IA,Mashanov GI,Molloy JE,Geeves MA,Mulvihill DP

    更新日期:2019-09-30 00:00:00

  • Visualization of stem cell activity in pancreatic cancer expansion by direct lineage tracing with live imaging.

    abstract::Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease. Although rigorous efforts identified the presence of 'cancer stem cells (CSCs)' in PDAC and molecular markers for them, stem cell dynamics in vivo have not been clearly demonstrated. Here we focused on Doublecortin-like kinase 1 (Dclk1), known as a CSC ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.55117

    authors: Maruno T,Fukuda A,Goto N,Tsuda M,Ikuta K,Hiramatsu Y,Ogawa S,Nakanishi Y,Yamaga Y,Yoshioka T,Takaori K,Uemoto S,Saur D,Chiba T,Seno H

    更新日期:2021-01-04 00:00:00

  • Akt-mTORC1 signaling regulates Acly to integrate metabolic input to control of macrophage activation.

    abstract::Macrophage activation/polarization to distinct functional states is critically supported by metabolic shifts. How polarizing signals coordinate metabolic and functional reprogramming, and the potential implications for control of macrophage activation, remains poorly understood. Here we show that IL-4 signaling co-opt...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.11612

    authors: Covarrubias AJ,Aksoylar HI,Yu J,Snyder NW,Worth AJ,Iyer SS,Wang J,Ben-Sahra I,Byles V,Polynne-Stapornkul T,Espinosa EC,Lamming D,Manning BD,Zhang Y,Blair IA,Horng T

    更新日期:2016-02-19 00:00:00

  • Dual midbrain and forebrain origins of thalamic inhibitory interneurons.

    abstract::The ubiquitous presence of inhibitory interneurons in the thalamus of primates contrasts with the sparsity of interneurons reported in mice. Here, we identify a larger than expected complexity and distribution of interneurons across the mouse thalamus, where all thalamic interneurons can be traced back to two developm...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.59272

    authors: Jager P,Moore G,Calpin P,Durmishi X,Salgarella I,Menage L,Kita Y,Wang Y,Kim DW,Blackshaw S,Schultz SR,Brickley S,Shimogori T,Delogu A

    更新日期:2021-02-01 00:00:00

  • RNA polymerase II stalling at pre-mRNA splice sites is enforced by ubiquitination of the catalytic subunit.

    abstract::Numerous links exist between co-transcriptional RNA processing and the transcribing RNAPII. In particular, pre-mRNA splicing was reported to be associated with slowed RNAPII elongation. Here, we identify a site of ubiquitination (K1246) in the catalytic subunit of RNAPII close to the DNA entry path. Ubiquitination was...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.27082

    authors: Milligan L,Sayou C,Tuck A,Auchynnikava T,Reid JE,Alexander R,Alves FL,Allshire R,Spanos C,Rappsilber J,Beggs JD,Kudla G,Tollervey D

    更新日期:2017-10-13 00:00:00

  • Embryonic and postnatal neurogenesis produce functionally distinct subclasses of dopaminergic neuron.

    abstract::Most neurogenesis in the mammalian brain is completed embryonically, but in certain areas the production of neurons continues throughout postnatal life. The functional properties of mature postnatally generated neurons often match those of their embryonically produced counterparts. However, we show here that in the ol...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.32373

    authors: Galliano E,Franzoni E,Breton M,Chand AN,Byrne DJ,Murthy VN,Grubb MS

    更新日期:2018-04-20 00:00:00

  • 5-HT2a receptor in mPFC influences context-guided reconsolidation of object memory in perirhinal cortex.

    abstract::Context-dependent memories may guide adaptive behavior relaying in previous experience while updating stored information through reconsolidation. Retrieval can be triggered by partial and shared cues. When the cue is presented, the most relevant memory should be updated. In a contextual version of the object recogniti...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.33746

    authors: Morici JF,Miranda M,Gallo FT,Zanoni B,Bekinschtein P,Weisstaub NV

    更新日期:2018-05-02 00:00:00

  • Neuropilin-1 functions as a VEGFR2 co-receptor to guide developmental angiogenesis independent of ligand binding.

    abstract::During development, tissue repair, and tumor growth, most blood vessel networks are generated through angiogenesis. Vascular endothelial growth factor (VEGF) is a key regulator of this process and currently both VEGF and its receptors, VEGFR1, VEGFR2, and Neuropilin1 (NRP1), are targeted in therapeutic strategies for ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.03720

    authors: Gelfand MV,Hagan N,Tata A,Oh WJ,Lacoste B,Kang KT,Kopycinska J,Bischoff J,Wang JH,Gu C

    更新日期:2014-09-22 00:00:00

  • Learning about loss.

    abstract::Researchers have identified two genes-follistatin and activin-as having an important role in the ability of certain flatworms to identify wounds that require the production of new tissue. ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.00533

    authors: Sánchez Alvarado A

    更新日期:2013-09-10 00:00:00

  • A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction.

    abstract::Two-dimensional (2D) human skeletal muscle fiber cultures are ill-equipped to support the contractile properties of maturing muscle fibers. This limits their application to the study of adult human neuromuscular junction (NMJ) development, a process requiring maturation of muscle fibers in the presence of motor neuron...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.44530

    authors: Afshar Bakooshli M,Lippmann ES,Mulcahy B,Iyer N,Nguyen CT,Tung K,Stewart BA,van den Dorpel H,Fuehrmann T,Shoichet M,Bigot A,Pegoraro E,Ahn H,Ginsberg H,Zhen M,Ashton RS,Gilbert PM

    更新日期:2019-05-14 00:00:00

  • Rescue of cognitive function following fractionated brain irradiation in a novel preclinical glioma model.

    abstract::More than half of long-term brain tumor survivors develop irreversible cognitive decline that severely affect their quality of life. However, there is no pre-clinical model that allows long-term assessment of cognition, and there is no treatment which ameliorates cognitive deficits in patients. Here, we report a novel...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.38865

    authors: Feng X,Liu S,Chen D,Rosi S,Gupta N

    更新日期:2018-11-13 00:00:00

  • The human gut and groundwater harbor non-photosynthetic bacteria belonging to a new candidate phylum sibling to Cyanobacteria.

    abstract::Cyanobacteria were responsible for the oxygenation of the ancient atmosphere; however, the evolution of this phylum is enigmatic, as relatives have not been characterized. Here we use whole genome reconstruction of human fecal and subsurface aquifer metagenomic samples to obtain complete genomes for members of a new c...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.01102

    authors: Di Rienzi SC,Sharon I,Wrighton KC,Koren O,Hug LA,Thomas BC,Goodrich JK,Bell JT,Spector TD,Banfield JF,Ley RE

    更新日期:2013-10-01 00:00:00

  • Evidence for transmission of COVID-19 prior to symptom onset.

    abstract::We collated contact tracing data from COVID-19 clusters in Singapore and Tianjin, China and estimated the extent of pre-symptomatic transmission by estimating incubation periods and serial intervals. The mean incubation periods accounting for intermediate cases were 4.91 days (95%CI 4.35, 5.69) and 7.54 (95%CI 6.76, 8...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.57149

    authors: Tindale LC,Stockdale JE,Coombe M,Garlock ES,Lau WYV,Saraswat M,Zhang L,Chen D,Wallinga J,Colijn C

    更新日期:2020-06-22 00:00:00

  • mPFC spindle cycles organize sparse thalamic activation and recently active CA1 cells during non-REM sleep.

    abstract::Sleep oscillations in the neocortex and hippocampus are critical for the integration of new memories into stable generalized representations in neocortex. However, the role of the thalamus in this process is poorly understood. To determine the thalamic contribution to non-REM oscillations (sharp-wave ripples, SWRs; sl...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.48881

    authors: Varela C,Wilson MA

    更新日期:2020-06-11 00:00:00

  • The life cycle of Drosophila orphan genes.

    abstract::Orphans are genes restricted to a single phylogenetic lineage and emerge at high rates. While this predicts an accumulation of genes, the gene number has remained remarkably constant through evolution. This paradox has not yet been resolved. Because orphan genes have been mainly analyzed over long evolutionary time sc...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.01311

    authors: Palmieri N,Kosiol C,Schlötterer C

    更新日期:2014-02-19 00:00:00

  • The cerebellum shows its stripes.

    abstract::New studies examine how the different sub-structures in the cerebellum are organized to receive information during complex behavioral tasks. ...

    journal_title:eLife

    pub_type: 评论,杂志文章

    doi:10.7554/eLife.52631

    authors: Holloway AL,Lerner TN

    更新日期:2019-11-14 00:00:00

  • The zinc-finger transcription factor Hindsight regulates ovulation competency of Drosophila follicles.

    abstract::Follicle rupture, the final step in ovulation, utilizes conserved molecular mechanisms including matrix metalloproteinases (Mmps), steroid signaling, and adrenergic signaling. It is still unknown how follicles become competent for follicle rupture/ovulation. Here, we identify a zinc-finger transcription factor Hindsig...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.29887

    authors: Deady LD,Li W,Sun J

    更新日期:2017-12-19 00:00:00

  • The extraembryonic serosa is a frontier epithelium providing the insect egg with a full-range innate immune response.

    abstract::Drosophila larvae and adults possess a potent innate immune response, but the response of Drosophila eggs is poor. In contrast to Drosophila, eggs of the beetle Tribolium are protected by a serosa, an extraembryonic epithelium that is present in all insects except higher flies. In this study, we test a possible immune...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.04111

    authors: Jacobs CG,Spaink HP,van der Zee M

    更新日期:2014-12-09 00:00:00

  • Local and global influences on protein turnover in neurons and glia.

    abstract::Regulation of protein turnover allows cells to react to their environment and maintain homeostasis. Proteins can show different turnover rates in different tissue, but little is known about protein turnover in different brain cell types. We used dynamic SILAC to determine half-lives of over 5100 proteins in rat primar...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.34202

    authors: Dörrbaum AR,Kochen L,Langer JD,Schuman EM

    更新日期:2018-06-19 00:00:00

  • Performing photosynthesis without β-carotene.

    abstract::Research on mutant tobacco plants shows that a pigment called β-carotene is not necessary for photosynthesis. ...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.63584

    authors: Telfer A

    更新日期:2020-11-03 00:00:00

  • Spatially resolved RNA-sequencing of the embryonic heart identifies a role for Wnt/β-catenin signaling in autonomic control of heart rate.

    abstract::Development of specialized cells and structures in the heart is regulated by spatially -restricted molecular pathways. Disruptions in these pathways can cause severe congenital cardiac malformations or functional defects. To better understand these pathways and how they regulate cardiac development we used tomo-seq, c...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.31515

    authors: Burkhard SB,Bakkers J

    更新日期:2018-02-05 00:00:00

  • Problems on the back of an envelope.

    abstract::Claims that magnetic fields can be used to manipulate biological systems contradict some basic laws of physics. ...

    journal_title:eLife

    pub_type: 评论,杂志文章

    doi:10.7554/eLife.19569

    authors: Anikeeva P,Jasanoff A

    更新日期:2016-09-08 00:00:00

  • A receptor and neuron that activate a circuit limiting sucrose consumption.

    abstract::The neural control of sugar consumption is critical for normal metabolism. In contrast to sugar-sensing taste neurons that promote consumption, we identify a taste neuron that limits sucrose consumption in Drosophila. Silencing of the neuron increases sucrose feeding; optogenetic activation decreases it. The feeding i...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.24992

    authors: Joseph RM,Sun JS,Tam E,Carlson JR

    更新日期:2017-03-23 00:00:00

  • Long-term potentiation is independent of the C-tail of the GluA1 AMPA receptor subunit.

    abstract::We tested the proposal that the C-terminal domain (CTD) of the AMPAR subunit GluA1 is required for LTP. We found that a knock-in mouse lacking the CTD of GluA1 expresses normal LTP and spatial memory, assayed by the Morris water maze. Our results support a model in which LTP generates synaptic slots, which capture pas...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.58042

    authors: Díaz-Alonso J,Morishita W,Incontro S,Simms J,Holtzman J,Gill M,Mucke L,Malenka RC,Nicoll RA

    更新日期:2020-08-24 00:00:00

  • Generalization of learned responses in the mormyrid electrosensory lobe.

    abstract::Appropriate generalization of learned responses to new situations is vital for adaptive behavior. We provide a circuit-level account of generalization in the electrosensory lobe (ELL) of weakly electric mormyrid fish. Much is already known in this system about a form of learning in which motor corollary discharge sign...

    journal_title:eLife

    pub_type: 杂志文章

    doi:10.7554/eLife.44032

    authors: Dempsey C,Abbott LF,Sawtell NB

    更新日期:2019-03-14 00:00:00